Determination of Antimicrobial, Antioxidant and Antibiofilm Activity of Some Alyssum L. Species in Anatolian Flora

Vedat Tozyılmaz; Ali Savaş Bülbül; Yusuf Ceylan

doi:10.18016/ksutarimdoga.vi.814167

Research Article

Determination of Antimicrobial, Antioxidant and Antibiofilm Activity of Some Alyssum L. Species in Anatolian Flora

Year 2021, Volume: 24 Issue: 4, 715 - 724, 31.08.2021

Vedat Tozyılmaz , Ali Savaş Bülbül , Yusuf Ceylan

https://doi.org/10.18016/ksutarimdoga.vi.814167

Cited By: 10

Abstract

The Alyssum L. genus which is spread in the Eastern Mediterranean is known by the public as ‘Rabid weed’ or ‘Kevke’. Many of these species are seen as a way to prevent disasters such as erosion, both due to drought resistance and low soil selectivity. Also Alyssum known as ‘Tooth weed’, it has been used to reduce tooth sore, bladder stones and spasm. In this study, in order to examine antibacterial and antifungal activities, extracts obtained from three naturally growing plants (Alyssum caricum T.R.Dudley and Hub.-Mor., Alyssum discolor T.R.Dudley and Hub.-Mor. and Alyssum sibiricum Willd.) in Turkey were examined on seventeen bacteria and two fungal strains by disc diffusion method. In addition, antimicrobial activity was supported by the Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC) method. DPPH (2,2-diphenyl-1-picrilhydrazyl) method was used to investigate the antioxidant activities of plant extracts, and crystal violet binding method was used to prevent biofilm formation. As a result, A. caricum ethanol extract showed the best effects in antimicrobial activities. None of plants were effective against fungi. A. sibiricum shows the best activity in antibiofilm activity and A. caricum was showed the best result in antioxidant activity.

Keywords

Alyssum discolor, Alyssum discolor, Alyssum discolor, Alyssum discolor, Alyssum caricum, Alyssum caricum, Alyssum caricum, Alyssum caricum, Alyssum sibiricum, Alyssum sibiricum, Alyssum sibiricum, Alyssum sibiricum, Antimicrobial activity, Antioxidant

Supporting Institution

Bartın Üniversitesi, BAP Koordinatörlüğü

Project Number

2017-FEN-CY-015

Thanks

This study was supported by Bartın University Scientific Research Office and the project number is 2017-FEN-CY-015 within the scope of the master's thesis research. We thank Prof Dr Handan UCUN ÖZEL for giving permission to use his laboratory at Bartın University Central Research Laboratory Research and Application Center.

References

Akagün G 2009. Alabaş (Brassica oleracea var. gongylodes) bitkisinin antioksidan aktivitesinin incelenmesi. Trakya Üniversitesi Fen Bilimleri Enstitüsü, Yüksek lisans tezi,106 sy.
Al-Shehbaz IA, Mutlu B, Dönmez AA 2007. The Brassicaceae (Cruciferae) of Turkey, updated. Turkish Journal of Botany, 31: 327-336.
Altuner EM, Çeter T, Gür M, Güney K, Kıran B, Akwieten HE, Soulman SI 2018. Chemical composition and antimicrobial activities of cold-pressed oils obtained from nettle, radish and pomegranate seeds. Kastamonu University Journal of Forestry Faculty, 18(3): 236-247.
Altuner EM, Çetin B 2018. Antimicrobial activity of Isothecium alopecuroides and potential effect of some climate elements on the activity of this bryophyte sample. Kastamonu University Journal of Forestry Faculty, 18(2): 126-137.
Atalan E, Bülbül AS, Ceylan Y 2020. Cephalarıa Syrıaca (L.): Investigation of antimicrobial, antibiofılm, antioxidant potential and seed morphology. Fresenius Environmental Bulletin, 29(5): 3641-3649.
Avato P, Argentieri MP 2015. Brassicaceae: A rich source of health improving phytochemicals. Phytochemistry Reviews, 14(6): 1019–1033.
Babaoğlu S, Bani B, Açık L, Adıgüzel N 2006. Taxonomic relations among some Turkish serpentine endemic Alyssum (Brassicaceae). Fifth International Balkan Botanical Congress, 20-26 June 2006, Sofia.
Benli M, Bingol U, Geven F, Guney K, Yigit N 2007. An Investigation on the antimicrobial activity of some endemic plant species from Turkey. African Journal of Biotechnology, 7(1).
Blois MS 1958. Antioxidant Determinations by the Use of a Stable Free Radical, Nature, 181(4617): 1199-1200.
Bülbül AS, Atalan E, Ulgen H, Ceylan KB 2018. The effect of kombucha fermentation on chestnut cancer factor (Cryphonectria parasitica (Murrill) E.M Barr). Kastamonu University, Journal of Forestry Faculty, 18(3): 304-313.
Bülbül AS, Ceylan Y, Armağan M 2018. Investigation of antibacterial and antifungal properties of Acanthophyllum acerosum and Acanthophyllum microcephalum. Research Journal of Biology Sciences. 11(2): 14-17.
Cartea ME, Francisco M, Soengas P, Velasco P 2011. Phenolic compounds in Brassica vegetables. Molecules 16: 251-280.
Ceyhan N 2008. Klinikte biyofilmlerin önlenmesi için antibiyofilm stratejileri, Infekt, 22: 227-240.
Couvreur TLP, Franzke A, Al-Shehbaz IA, Bakker FT, Koch MA, Mummenhoff K 2010. Molecular phylogenetics, temporal diversification and principles of evolution in the mustard family (Brassicaceae), Molecular Biology and Evolution, 27: 55-71.
Dağcı EK, Dığrak M 2005. Bazı meyve ekstraktlarının antibakteriyal ve antifungal aktiviteleri. KSU. J. Sci. and Eng, 8: 1-8.
Duthie GG, Duthie SJ, Kyle JAM 2000. Plant polyphenols in cancer and heart disease: implications as nutritional antioxidants. Nutrition Research Reviews. 13: 79–106.
Erdogan AE, Everest A 2012. Antimikrobiyal ajan olarak bitki bileşenleri. Türk Bilimsel Derlemeler Dergisi, 6(2): 27-32.
Erdogrul ÖT 2002. Antibacterial activities of some plant extracts used in folk medicine. Pharmaceutical Biology, 40(4), 269-273.
Franklin MJ, Chang C, Akiyama T, Bothner B 2015. New technologies for studying biofilms. Microbiology spectrum, 3(4).
Gülçin İ 2006. Antioxidant activity of caffeic acid (3, 4-dihydroxycinnamic acid). Toxicology, 217(2-3): 213-220.
Huang D, Ou B, Prior RL 2005. The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry, 53: 1841-1856.
Iwu GMW, Duncan AB, Okuuji CO 1999. New Antimicrobials of Plant Orijin. ASHS Pres, Alexandria, 457-462 Kokare CR, Chakraborty S, Khobade AN, Mahadik KR 2009. Biofilms: Importance and Applications. Indian J Biotechnology, 8: 159-168.
Kumar K, Gupta SM, Arya MC, Nasim M 2017. In vitro antimicrobial and antioxidant activity of camelina seed extracts as potential source of bioactive compounds. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 87(2): 521-526.
March JC, Bentley WE 2004. Quorum sensing and bacterial cross-talk in biotechnology. Curr Opin Biotech, 15(5): 495–502.
Martinez-Sanchez A, Gil-Izquierdo A, Gil MI, Ferreres F 2008. A comparative study of flavonoid compounds, vitamin C, and antioxidant properties of baby leaf Brassicaceae species. Journal of Agricultural and Food Chemistry, 56(7): 2330-2340.
Mutlu B, Karakuş Ş 2012. A new species of Ornithogalum (Hyacinthaceae) from East Anatolia, Turkey, Turkish Journal of Biology, 36: 125-133.
Oliveira A, Cataneli Pereira V, Pinheiro L, Moraes Riboli DF, Ribeiro de Souza BMK, Cunha M de L 2016. Antimicrobial resistance profile of planktonic and biofilm cells of Staphylococcus aureus and coagulase-negative staphylococci. International Journal of Molecular Sciences. 17: E1423, 118: 133-140.
Pandey KB, Rizvi SI 2009. Plant polyphenols as dietary antioxidants in human health and disease. Oxidative Medicine and Cellular Longevity. 2: 270-278.
Shinji M 1993. Research on antibiotic screening in japan over the last decade: a producing microorganism approach. Actinomycetol. 7: 100-106.
Taga ME, Bassler BL 2003. Chemical Communication Among Bacteria. Proc. Natl. Acad. Sci. USA, 100(2): 14549–14554.
Tozyılmaz V, Bulbul AS 2018. Antibacterial effects of Alyssum L. against some gram-positive and gram-negative bacteria, International Symposium Ecology, 19-23 June 2018, Kastamonu.
Tozyılmaz V, Bulbul AS, Ceylan Y, Armagan M 2020. Antibacterial, antifungal, antibiofilm and antioxidant activities of some endemic plants in Anatolian flora. Fresenius Environmental Bulletin, 29(6): 4338-4346.
Ulgen H, Bulbul AS, Ceylan KB 2020. Investigation of antimicrobial, antibiofilm, antioxidant potential and seed morphology of Camelına Satıva L. Crantz. Fresenius Environmental Bulletin, 29(7): 5121-5129.
Yaldiz G, Kulak M 2014. Assessment on adaptation of some selected medicinal and aromatic plants to the northern parts of Turkey: Agricultural and chemical property based evaluation. Medicinal and Aromatic Plant Research Journal, 2(3): 50-56.

Anadolu Florasına Ait Bazı Alyssum L. Türlerinin Antimikrobiyal, Antioksidan ve Antibiyofilm Aktivitesinin Belirlenmesi

Year 2021, Volume: 24 Issue: 4, 715 - 724, 31.08.2021

Vedat Tozyılmaz , Ali Savaş Bülbül , Yusuf Ceylan

https://doi.org/10.18016/ksutarimdoga.vi.814167

Cited By: 10

Abstract

Doğu Akdeniz’de yayılış gösteren Alyssum L. cinsi, halk tarafından ‘Kuduz otu’ veya ‘Kevke’ olarak bilinir. Alyssum türlerinin çoğu kuraklığa karşı dayanıklı olması ve toprak seçimlerinin olmaması sebebi ile erozyon gibi felaketleri önlemede önemlidir. Ayrıca Alyssum diş otu olarak bilinmekte olup diş ağrılarında, spazm ve mesane taşlarını düşürmede kullanılan bir bitkidir. Bu çalışmada, antibakteriyel ve antifungal aktiviteleri incelemek için ülkemizde doğal olarak yetişen üç bitkiden (Alyssum caricum T.R.Dudley ve Hub.-Mor., Alyssum discolor T.R.Dudley ve Hub.-Mor. ve Alyssum sibiricum Willd.) elde edilen ekstraktlar, disk difüzyon yöntemi ile on yedi bakteri ve iki mantar suşu üzerinde incelenmiştir. Ayrıca, antimikrobiyal aktivite Minimum İnhibisyon Konsantrasyonu (MIC) ve Minimum Bakterisidal Konsantrasyonu (MBC) yöntemi ile desteklenmiştir. Bitki ekstraktlarının antioksidan aktivitelerini araştırmak için DPPH (2,2-difenil-1-pikrilhidrazil) yöntemi, biyofilm oluşumunu önlemek için ise kristal viyole bağlama yöntemi kullanılmıştır. Sonuç olarak, antimikrobiyal çalışmasında en iyi sonucu A. caricum etanol ekstraktı gösterdi. Bitkilerin hiçbiri funguslara karşı etki gösteremedi. Antibiyofilm çalışmasında en iyi sonucu A. sibiricum gösterirken antioksidan çalışmasında ise en iyi aktiviteyi A. caricum ekstraktı gösterdiği tespit edildi.

Keywords

Alyssum discolor, Alyssum caricum, Alyssum sibiricum, Antimikrobiyal aktivite, Antioksidant

Project Number

2017-FEN-CY-015

References

Akagün G 2009. Alabaş (Brassica oleracea var. gongylodes) bitkisinin antioksidan aktivitesinin incelenmesi. Trakya Üniversitesi Fen Bilimleri Enstitüsü, Yüksek lisans tezi,106 sy.
Al-Shehbaz IA, Mutlu B, Dönmez AA 2007. The Brassicaceae (Cruciferae) of Turkey, updated. Turkish Journal of Botany, 31: 327-336.
Altuner EM, Çeter T, Gür M, Güney K, Kıran B, Akwieten HE, Soulman SI 2018. Chemical composition and antimicrobial activities of cold-pressed oils obtained from nettle, radish and pomegranate seeds. Kastamonu University Journal of Forestry Faculty, 18(3): 236-247.
Altuner EM, Çetin B 2018. Antimicrobial activity of Isothecium alopecuroides and potential effect of some climate elements on the activity of this bryophyte sample. Kastamonu University Journal of Forestry Faculty, 18(2): 126-137.
Atalan E, Bülbül AS, Ceylan Y 2020. Cephalarıa Syrıaca (L.): Investigation of antimicrobial, antibiofılm, antioxidant potential and seed morphology. Fresenius Environmental Bulletin, 29(5): 3641-3649.
Avato P, Argentieri MP 2015. Brassicaceae: A rich source of health improving phytochemicals. Phytochemistry Reviews, 14(6): 1019–1033.
Babaoğlu S, Bani B, Açık L, Adıgüzel N 2006. Taxonomic relations among some Turkish serpentine endemic Alyssum (Brassicaceae). Fifth International Balkan Botanical Congress, 20-26 June 2006, Sofia.
Benli M, Bingol U, Geven F, Guney K, Yigit N 2007. An Investigation on the antimicrobial activity of some endemic plant species from Turkey. African Journal of Biotechnology, 7(1).
Blois MS 1958. Antioxidant Determinations by the Use of a Stable Free Radical, Nature, 181(4617): 1199-1200.
Bülbül AS, Atalan E, Ulgen H, Ceylan KB 2018. The effect of kombucha fermentation on chestnut cancer factor (Cryphonectria parasitica (Murrill) E.M Barr). Kastamonu University, Journal of Forestry Faculty, 18(3): 304-313.
Bülbül AS, Ceylan Y, Armağan M 2018. Investigation of antibacterial and antifungal properties of Acanthophyllum acerosum and Acanthophyllum microcephalum. Research Journal of Biology Sciences. 11(2): 14-17.
Cartea ME, Francisco M, Soengas P, Velasco P 2011. Phenolic compounds in Brassica vegetables. Molecules 16: 251-280.
Ceyhan N 2008. Klinikte biyofilmlerin önlenmesi için antibiyofilm stratejileri, Infekt, 22: 227-240.
Couvreur TLP, Franzke A, Al-Shehbaz IA, Bakker FT, Koch MA, Mummenhoff K 2010. Molecular phylogenetics, temporal diversification and principles of evolution in the mustard family (Brassicaceae), Molecular Biology and Evolution, 27: 55-71.
Dağcı EK, Dığrak M 2005. Bazı meyve ekstraktlarının antibakteriyal ve antifungal aktiviteleri. KSU. J. Sci. and Eng, 8: 1-8.
Duthie GG, Duthie SJ, Kyle JAM 2000. Plant polyphenols in cancer and heart disease: implications as nutritional antioxidants. Nutrition Research Reviews. 13: 79–106.
Erdogan AE, Everest A 2012. Antimikrobiyal ajan olarak bitki bileşenleri. Türk Bilimsel Derlemeler Dergisi, 6(2): 27-32.
Erdogrul ÖT 2002. Antibacterial activities of some plant extracts used in folk medicine. Pharmaceutical Biology, 40(4), 269-273.
Franklin MJ, Chang C, Akiyama T, Bothner B 2015. New technologies for studying biofilms. Microbiology spectrum, 3(4).
Gülçin İ 2006. Antioxidant activity of caffeic acid (3, 4-dihydroxycinnamic acid). Toxicology, 217(2-3): 213-220.
Huang D, Ou B, Prior RL 2005. The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry, 53: 1841-1856.
Iwu GMW, Duncan AB, Okuuji CO 1999. New Antimicrobials of Plant Orijin. ASHS Pres, Alexandria, 457-462 Kokare CR, Chakraborty S, Khobade AN, Mahadik KR 2009. Biofilms: Importance and Applications. Indian J Biotechnology, 8: 159-168.
Kumar K, Gupta SM, Arya MC, Nasim M 2017. In vitro antimicrobial and antioxidant activity of camelina seed extracts as potential source of bioactive compounds. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 87(2): 521-526.
March JC, Bentley WE 2004. Quorum sensing and bacterial cross-talk in biotechnology. Curr Opin Biotech, 15(5): 495–502.
Martinez-Sanchez A, Gil-Izquierdo A, Gil MI, Ferreres F 2008. A comparative study of flavonoid compounds, vitamin C, and antioxidant properties of baby leaf Brassicaceae species. Journal of Agricultural and Food Chemistry, 56(7): 2330-2340.
Mutlu B, Karakuş Ş 2012. A new species of Ornithogalum (Hyacinthaceae) from East Anatolia, Turkey, Turkish Journal of Biology, 36: 125-133.
Oliveira A, Cataneli Pereira V, Pinheiro L, Moraes Riboli DF, Ribeiro de Souza BMK, Cunha M de L 2016. Antimicrobial resistance profile of planktonic and biofilm cells of Staphylococcus aureus and coagulase-negative staphylococci. International Journal of Molecular Sciences. 17: E1423, 118: 133-140.
Pandey KB, Rizvi SI 2009. Plant polyphenols as dietary antioxidants in human health and disease. Oxidative Medicine and Cellular Longevity. 2: 270-278.
Shinji M 1993. Research on antibiotic screening in japan over the last decade: a producing microorganism approach. Actinomycetol. 7: 100-106.
Taga ME, Bassler BL 2003. Chemical Communication Among Bacteria. Proc. Natl. Acad. Sci. USA, 100(2): 14549–14554.
Tozyılmaz V, Bulbul AS 2018. Antibacterial effects of Alyssum L. against some gram-positive and gram-negative bacteria, International Symposium Ecology, 19-23 June 2018, Kastamonu.
Tozyılmaz V, Bulbul AS, Ceylan Y, Armagan M 2020. Antibacterial, antifungal, antibiofilm and antioxidant activities of some endemic plants in Anatolian flora. Fresenius Environmental Bulletin, 29(6): 4338-4346.
Ulgen H, Bulbul AS, Ceylan KB 2020. Investigation of antimicrobial, antibiofilm, antioxidant potential and seed morphology of Camelına Satıva L. Crantz. Fresenius Environmental Bulletin, 29(7): 5121-5129.
Yaldiz G, Kulak M 2014. Assessment on adaptation of some selected medicinal and aromatic plants to the northern parts of Turkey: Agricultural and chemical property based evaluation. Medicinal and Aromatic Plant Research Journal, 2(3): 50-56.

There are 34 citations in total.

Details

Primary Language	English
Subjects	Structural Biology
Journal Section	RESEARCH ARTICLE
Authors	Vedat Tozyılmaz 0000-0003-0799-0089 Ali Savaş Bülbül 0000-0002-2200-7348 Yusuf Ceylan 0000-0001-8186-7252
Project Number	2017-FEN-CY-015
Publication Date	August 31, 2021
Submission Date	October 21, 2020
Acceptance Date	December 3, 2020
Published in Issue	Year 2021Volume: 24 Issue: 4

Cite

APA	Tozyılmaz, V., Bülbül, A. S., & Ceylan, Y. (2021). Determination of Antimicrobial, Antioxidant and Antibiofilm Activity of Some Alyssum L. Species in Anatolian Flora. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(4), 715-724. https://doi.org/10.18016/ksutarimdoga.vi.814167