Determination of Antimicrobial, Antioxidant and Antibiofilm Activity of Some Alyssum L. Species in Anatolian Flora

Vedat Tozyılmaz; Ali Savaş Bülbül; Yusuf Ceylan

doi:10.18016/ksutarimdoga.vi.814167

TR EN

Anadolu Florasına Ait Bazı Alyssum L. Türlerinin Antimikrobiyal, Antioksidan ve Antibiyofilm Aktivitesinin Belirlenmesi

Öz

Doğu Akdeniz’de yayılış gösteren Alyssum L. cinsi, halk tarafından ‘Kuduz otu’ veya ‘Kevke’ olarak bilinir. Alyssum türlerinin çoğu kuraklığa karşı dayanıklı olması ve toprak seçimlerinin olmaması sebebi ile erozyon gibi felaketleri önlemede önemlidir. Ayrıca Alyssum diş otu olarak bilinmekte olup diş ağrılarında, spazm ve mesane taşlarını düşürmede kullanılan bir bitkidir. Bu çalışmada, antibakteriyel ve antifungal aktiviteleri incelemek için ülkemizde doğal olarak yetişen üç bitkiden (Alyssum caricum T.R.Dudley ve Hub.-Mor., Alyssum discolor T.R.Dudley ve Hub.-Mor. ve Alyssum sibiricum Willd.) elde edilen ekstraktlar, disk difüzyon yöntemi ile on yedi bakteri ve iki mantar suşu üzerinde incelenmiştir. Ayrıca, antimikrobiyal aktivite Minimum İnhibisyon Konsantrasyonu (MIC) ve Minimum Bakterisidal Konsantrasyonu (MBC) yöntemi ile desteklenmiştir. Bitki ekstraktlarının antioksidan aktivitelerini araştırmak için DPPH (2,2-difenil-1-pikrilhidrazil) yöntemi, biyofilm oluşumunu önlemek için ise kristal viyole bağlama yöntemi kullanılmıştır. Sonuç olarak, antimikrobiyal çalışmasında en iyi sonucu A. caricum etanol ekstraktı gösterdi. Bitkilerin hiçbiri funguslara karşı etki gösteremedi. Antibiyofilm çalışmasında en iyi sonucu A. sibiricum gösterirken antioksidan çalışmasında ise en iyi aktiviteyi A. caricum ekstraktı gösterdiği tespit edildi.

Anahtar Kelimeler

Determination of Antimicrobial, Antioxidant and Antibiofilm Activity of Some Alyssum L. Species in Anatolian Flora

Abstract

The Alyssum L. genus which is spread in the Eastern Mediterranean is known by the public as ‘Rabid weed’ or ‘Kevke’. Many of these species are seen as a way to prevent disasters such as erosion, both due to drought resistance and low soil selectivity. Also Alyssum known as ‘Tooth weed’, it has been used to reduce tooth sore, bladder stones and spasm. In this study, in order to examine antibacterial and antifungal activities, extracts obtained from three naturally growing plants (Alyssum caricum T.R.Dudley and Hub.-Mor., Alyssum discolor T.R.Dudley and Hub.-Mor. and Alyssum sibiricum Willd.) in Turkey were examined on seventeen bacteria and two fungal strains by disc diffusion method. In addition, antimicrobial activity was supported by the Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC) method. DPPH (2,2-diphenyl-1-picrilhydrazyl) method was used to investigate the antioxidant activities of plant extracts, and crystal violet binding method was used to prevent biofilm formation. As a result, A. caricum ethanol extract showed the best effects in antimicrobial activities. None of plants were effective against fungi. A. sibiricum shows the best activity in antibiofilm activity and A. caricum was showed the best result in antioxidant activity.

Keywords

Destekleyen Kurum

Bartın Üniversitesi, BAP Koordinatörlüğü

Proje Numarası

2017-FEN-CY-015

Teşekkür

This study was supported by Bartın University Scientific Research Office and the project number is 2017-FEN-CY-015 within the scope of the master's thesis research. We thank Prof Dr Handan UCUN ÖZEL for giving permission to use his laboratory at Bartın University Central Research Laboratory Research and Application Center.

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Vedat Tozyılmaz
0000-0003-0799-0089
Türkiye

Ali Savaş Bülbül ^*
0000-0002-2200-7348
Türkiye

Yusuf Ceylan
0000-0001-8186-7252
Türkiye

Yayımlanma Tarihi

31 Ağustos 2021

Gönderilme Tarihi

21 Ekim 2020

Kabul Tarihi

3 Aralık 2020

Yayımlandığı Sayı

Yıl 2021 Cilt: 24 Sayı: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.814167

IZ

https://izlik.org/JA29RU62LW

Kaynak Göster

RIS / Bibtex

APA

Tozyılmaz, V., Bülbül, A. S., & Ceylan, Y. (2021). Determination of Antimicrobial, Antioxidant and Antibiofilm Activity of Some Alyssum L. Species in Anatolian Flora. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 24(4), 715-724. https://doi.org/10.18016/ksutarimdoga.vi.814167

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Determination of Antimicrobial, Antioxidant and Antibiofilm Activity of Some Alyssum L. Species in Anatolian Flora

Anadolu Florasına Ait Bazı Alyssum L. Türlerinin Antimikrobiyal, Antioksidan ve Antibiyofilm Aktivitesinin Belirlenmesi

Öz

Anahtar Kelimeler

Determination of Antimicrobial, Antioxidant and Antibiofilm Activity of Some Alyssum L. Species in Anatolian Flora

Abstract

Keywords

Destekleyen Kurum

Proje Numarası

Teşekkür

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

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The Relationship Between Antimicrobial Activities and Mineral Contents of Narrow Endemic Gypsophytes and Their Chemical Contents

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Micromorphological considerations on Alyssum nezaketiae Aytaç & H. Duman (Brassicaceae), endemic to gypsum habitats from Turkey: An electron microscopic study

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e-ISSN: 2619-9149