Research Article
Determination of the Effect of Tebuconazole Applications on Cucumber (Cucumis sativus L.) Seedling via Morphological and Molecular Methods
Abstract
Aim of this study was to determine the effect of tebuconazole applications on cucumber seedling via morphological and molecular methods. In order to determine the most appropriate dose of different concentrations (25, 50, 75, 100, 125 and 150 ppm) of tebuconazole affecting the seedling quality and preventing possible genotoxic effects, retrotransposon motility at all doses was determined. Polymorphism rates and changes in GTS values were also determined. At the applied doses, polymorphism values were found as 0%, 5.55%, 16.66%, 38.88%, 55.55% and 61.11%, respectively. The GTS value was decreased from 100% to approximately 38.89% based on the applied doses. Statistical evaluations indicated that different dose applications resulted significant changes in the seedling characteristics. The seedling and stem heights were suppressed by 57.5% and 55.9%, respectively compared to the controls. The stem diameter increased 9.95% following the application. Overall, 50, 75 and 150 ppm doses were determined to achieve an increase in the dry matter and leaf chlorophyll content. In this study, applications of Tebuconazole at different doses controled the seedling height in cucumber and affected specific quality characteristics of the seedlings.
Keywords
Thanks
Thank you for the contributions of the Erzincan Horticultural Research Institute.
References
- Akdemir S 2018. Marul (Lactuca sativa L.) fide kalitesi ve bitki gelişimi üzerine paclobutrazol ve prohexadione-calcium uygulamalarının etkileri., Kırşehir Ahi Evran Üniversitesi, Fen Bilimleri Enstitüsü, Tarımsal Biyoteknoloji Anabilim Dalı, Yüksek Lisans Tezi, 103 Sy, Kırşehir.
- Baninasab B 2009. Amelioration of chilling stress by paclobutrazol in watermelon seedlings. Scientia Horticulturae, 121(2): 144-148.
- Bennetzen JL 2000. Transposable elements contributions to plant gene and genome evolution. Plant Molecular Biology, 42: 251-269. https://doi.org/10.1023/A:1006344508454
- Berova M, Zlatev Z 2000. Physiological response and yield of paclobutrazol treated tomato plants (Lycopersicon esculentum Mill.). Plant Growth Regulation, 30(2): 117-123.
- Brigard JP, Harkess RL, Baldwin BS 2006. Tomato early seedling height control using a paclobutrazol seed soak. Horticultural Science, 41(3): 768-772.
- Cadkova E, Komarek M, Kaliszova R, Vanek A, Balikova, M 2013. Tebuconazole sorption in contrasting soil types. Soil and Sediment Contamination: An International Journal, 22(4): 404-414.
- Çopur H, Nebahat S 2011. Sera Hıyar Fidesi Üretiminde Paclobutrazol ve Bakır Sülfat Uygulamalarının Fide Büyümesi Üzerine Etkileri. Çukurova Üniversitesi Ziraat Fakültesi Dergisi, 27(1): 1-12.
- Demir İ, Balkaya A, Yılmaz K, Onus AN, Uyanık M, Kaycıoğlu M, Bozkurt B 2010. Sebzelerde tohumluk ve fide üretimi. TMMOB Ziraat Mühendisleri Odası, VII. Türkiye Ziraat Mühendisliği Teknik Kongresi, 11-15 Ocak 2010, Ankara.
- Federoff N 2000. Transposons and Genome Evolutions in Plants. Proceedings of the National Academy of Sciences, 97 (13): 7002-7007.
- Garner LC, Björkman T 1996. Mechanical conditioning for controlling excessive elongation in tomato transplants: sensitivity to dose, frequency, and timing of brushing. Journal of the American Society for Horticultural Science,121(5): 894-900.
- Geboloğlu N, Durukan A, Sağlam N, Doksöz S, Şahin S, Yılmaz E 2015. Patlıcanda Fide Gelişimi ve Fide Kalitesi ile Paclobutrazol Uygulamaları Arasındaki İlişkiler. International Journal of Agricultural and Natural Sciences (IJANS) E-ISSN: 2651-3617, 8(1): 62-66.
- Geboloğlu N, Kum AD, Şahin S, Boncukçu SD, Sağlam N 2016. Paklobutrazolun Marulda Fide Boyu ve Kalite Özelliklerine Etkisi. International Journal of Agricultural and Natural Sciences (IJANS) E- ISSN: 2651-3617, 9(2): 26-29.
- Głowacka B 2004. The effect of blue light on the height and habit of the tomato Lycopersicon esculentum Mill.) transplant. Folia Horticulturae, 16(2): 3-10.
- Hamad-Mecbur H, Yilmaz S, Temel A, Sahin K, Gozukirmizi N 2014. Effects of epirubicin on barley seedlings. Toxicology Indiana Health, 30: 52-59.
- Johjima T, Latimer JG, Wakita H 1992. Brushing influences transplant growth and subsequent yield of four cultivars of tomato and their hybrid lines. Journal of the American Society for Horticultural Science, 117(3): 384- 388.
- Kılıç O, Çopur U, Göktay S (1991) Meyve ve Sebze İşleme Teknolojisi Uygulama Kılavuzu. Uludağ Üniversitesi Ziraat Fakültesi Ders Notları:7, s:143.
- Melton RR, Dufault RJ 1991. Tomato seedling growth, earliness, yield, and quality following pretransplant nutritional conditioning and low temperatures. Journal of the American Society for Horticultural Science, 116(3): 421-425.
- Mohsin SM, Hasanuzzaman M, Bhuyan MHM, Parvin K, Fujita M 2019. Exogenous tebuconazole and trifloxystrobin regulates reactive oxygen species metabolism toward mitigating salt-induced damages in cucumber seedling. Plants, 8(10): 428.
- Moraes PJ, Grossi JAS, de Araújo Tinoco S, da Silva DJH, Cecon PR, Barbosa JG 2005. Ornamental tomato growth and fruiting response to paclobutrazol. Acta Horticulturae, 683: 327.
- Rogach VV, Voytenko LV, Shcherbatiuk MM, Kosakivska IV, Rogach TI, 2020. Morphogenesis, pigment content, phytohormones and productivity of eggplants under the action of gibberellin and tebuconazole. Regulatory Mechanisms in Biosystems, 11(1): 116-122.
- Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW 1984. Ribosomal DNAsepacer-length polymorphism in barley: mendelian inheritance, chromosomal location, and population Dynamics. Proceedings of the National Academy Sciences, 81: 8014-8019.
- Sönmez İ 2017. Atık mantar kompostunun domates fidelerinin gelişimi ve besin içerikleri üzerine olan etkilerinin belirlenmesi. Mediterranean Agricultural Sciences, 30(1): 59-63.
- Sunar S, Bulut H 2019. Buğday (Triticum aestivum L.) Tohumlarında Büyüme Düzenleyicisi 2,4-D Isooctylester Herbisitinin Meydana Getirdiği Retrotranspozon Hareketliliğinin Moleküler Yöntem ile Değerlendirilmesi. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 12(2): 585-594.
- Temel A, Gözükirmizi N 2013. Analysis of retrotransposition and DNA methylation in barley callus culture. Acta Biologica Hungarica, 64 (1): 86-95.
- Teto AA, Laubscher CP, Ndakidemi PA, Matimati I 2016. Paclobutrazol retards vegetative growth in hydroponically-cultured Leonotis leonurus (L.) R. Br. Lamiaceae for a multipurpose flowering potted plant. South African Journal of Botany, 106: 67-70.
- Wessler SR 2009. Eukaryotic transposable elements: teaching old genomes new tricks’’, http://www.plantbio. uga. edu/ wesslerlab/The_Implicit_Genome.pdf, Son Erişim Tarihi: 10.03.2017.
- Wicker T, Sabot F, Hua-Van A, Bennetzen J, Capy P, Chalhoub B, Flavell A, Leroy P, Michele M, Olivier P, E Paux, Phillip S, Alan HS 2007. A Unified Classification System for Eukaryotic Transposable Elements. Nature Genetics, 8: 973-982.
- Yigider E, Taspinar MS, Sigmaz B, Aydin M, Agar G 2016. Humic acids protective activity against manganese induced LTR (long terminal repeat) retrotransposon polymorphism and genomic instability effects in Zea mays. Plant Gene, 6: 13-17.
- Zandstra JW, Squire RC, Watt GJ 2007. Managing transplant size and advancing field maturity of fresh tomatoes and peppers. In Ontario vegetable crop research: University of Guelph Ridgetown Campus, pp. 1-16.
Tebuconazole Uygulamalarının Hıyar (Cucumis sativus L.) Fidelerindeki Etkisinin Morfolojik ve Moleküler Yöntemler ile Belirlenmesi
Abstract
Çalışmada, Tebuconazole uygulamalarının hıyar (Cucumis sativus L.) fidelerindeki etkisinin morfolojik ve moleküler yöntemler ile belirlenmesi amaçlanmıştır. Yapraktan sprey şeklinde uygulanan Tebuconazole’ün 25, 50, 75, 100, 125 ve 150 ppm konsantrasyonları denenmiştir. Fide kalitesine olumlu etki eden en uygun dozun belirlenmesi ve muhtemel genotoksik etkilerin engellenmesi için tüm dozlardaki retrotranspozon hareketliliği tespit edilmiştir. Ayrıca oluşan polimorfizm oranları ve GTS değerlerindeki değişim belirlenmiştir. Uygulanan dozlarda sırasıyla %0, %5.55, %16.66, %38.88, %55.55 ve %61.11 oranında polimorfizm elde edilmiştir. GTS değerinin ise doza bağlı olarak %100 seviyesinden %38.89 seviyesine gerilediği görülmüştür. Yapılan istatiksel değerlendirmelere göre, farklı doz uygulamalarının fide özelliklerinde önemli derecede değişiklik meydana getirdiği tespit edilmiştir. Elde edilen verilere göre, kontrol uygulaması ile karşılaştırıldığında, fide boyu ve gövde boyunda sırası ile %57,5 ve %55,9’luk bir azalma sağlanmıştır. Gövde çapında ise uygulama sonrası %9.95’lik bir artış olmuştur. 50, 75 ve 150 ppm’lik dozların gövde kuru madde oranında artış sağladığı belirlenmiştir. Ayrıca, doz uygulaması yaprak klorofil miktarında bir artış sağlamıştır. Bu çalışmada, farklı dozlarda Tebuconazole uygulamalarının, hıyarda fide boyunu kontrol altına aldığı ve bazı fide kalite özelliklerine olumlu yönde etki ettiği belirlenmiştir.
Keywords
References
- Akdemir S 2018. Marul (Lactuca sativa L.) fide kalitesi ve bitki gelişimi üzerine paclobutrazol ve prohexadione-calcium uygulamalarının etkileri., Kırşehir Ahi Evran Üniversitesi, Fen Bilimleri Enstitüsü, Tarımsal Biyoteknoloji Anabilim Dalı, Yüksek Lisans Tezi, 103 Sy, Kırşehir.
- Baninasab B 2009. Amelioration of chilling stress by paclobutrazol in watermelon seedlings. Scientia Horticulturae, 121(2): 144-148.
- Bennetzen JL 2000. Transposable elements contributions to plant gene and genome evolution. Plant Molecular Biology, 42: 251-269. https://doi.org/10.1023/A:1006344508454
- Berova M, Zlatev Z 2000. Physiological response and yield of paclobutrazol treated tomato plants (Lycopersicon esculentum Mill.). Plant Growth Regulation, 30(2): 117-123.
- Brigard JP, Harkess RL, Baldwin BS 2006. Tomato early seedling height control using a paclobutrazol seed soak. Horticultural Science, 41(3): 768-772.
- Cadkova E, Komarek M, Kaliszova R, Vanek A, Balikova, M 2013. Tebuconazole sorption in contrasting soil types. Soil and Sediment Contamination: An International Journal, 22(4): 404-414.
- Çopur H, Nebahat S 2011. Sera Hıyar Fidesi Üretiminde Paclobutrazol ve Bakır Sülfat Uygulamalarının Fide Büyümesi Üzerine Etkileri. Çukurova Üniversitesi Ziraat Fakültesi Dergisi, 27(1): 1-12.
- Demir İ, Balkaya A, Yılmaz K, Onus AN, Uyanık M, Kaycıoğlu M, Bozkurt B 2010. Sebzelerde tohumluk ve fide üretimi. TMMOB Ziraat Mühendisleri Odası, VII. Türkiye Ziraat Mühendisliği Teknik Kongresi, 11-15 Ocak 2010, Ankara.
- Federoff N 2000. Transposons and Genome Evolutions in Plants. Proceedings of the National Academy of Sciences, 97 (13): 7002-7007.
- Garner LC, Björkman T 1996. Mechanical conditioning for controlling excessive elongation in tomato transplants: sensitivity to dose, frequency, and timing of brushing. Journal of the American Society for Horticultural Science,121(5): 894-900.
- Geboloğlu N, Durukan A, Sağlam N, Doksöz S, Şahin S, Yılmaz E 2015. Patlıcanda Fide Gelişimi ve Fide Kalitesi ile Paclobutrazol Uygulamaları Arasındaki İlişkiler. International Journal of Agricultural and Natural Sciences (IJANS) E-ISSN: 2651-3617, 8(1): 62-66.
- Geboloğlu N, Kum AD, Şahin S, Boncukçu SD, Sağlam N 2016. Paklobutrazolun Marulda Fide Boyu ve Kalite Özelliklerine Etkisi. International Journal of Agricultural and Natural Sciences (IJANS) E- ISSN: 2651-3617, 9(2): 26-29.
- Głowacka B 2004. The effect of blue light on the height and habit of the tomato Lycopersicon esculentum Mill.) transplant. Folia Horticulturae, 16(2): 3-10.
- Hamad-Mecbur H, Yilmaz S, Temel A, Sahin K, Gozukirmizi N 2014. Effects of epirubicin on barley seedlings. Toxicology Indiana Health, 30: 52-59.
- Johjima T, Latimer JG, Wakita H 1992. Brushing influences transplant growth and subsequent yield of four cultivars of tomato and their hybrid lines. Journal of the American Society for Horticultural Science, 117(3): 384- 388.
- Kılıç O, Çopur U, Göktay S (1991) Meyve ve Sebze İşleme Teknolojisi Uygulama Kılavuzu. Uludağ Üniversitesi Ziraat Fakültesi Ders Notları:7, s:143.
- Melton RR, Dufault RJ 1991. Tomato seedling growth, earliness, yield, and quality following pretransplant nutritional conditioning and low temperatures. Journal of the American Society for Horticultural Science, 116(3): 421-425.
- Mohsin SM, Hasanuzzaman M, Bhuyan MHM, Parvin K, Fujita M 2019. Exogenous tebuconazole and trifloxystrobin regulates reactive oxygen species metabolism toward mitigating salt-induced damages in cucumber seedling. Plants, 8(10): 428.
- Moraes PJ, Grossi JAS, de Araújo Tinoco S, da Silva DJH, Cecon PR, Barbosa JG 2005. Ornamental tomato growth and fruiting response to paclobutrazol. Acta Horticulturae, 683: 327.
- Rogach VV, Voytenko LV, Shcherbatiuk MM, Kosakivska IV, Rogach TI, 2020. Morphogenesis, pigment content, phytohormones and productivity of eggplants under the action of gibberellin and tebuconazole. Regulatory Mechanisms in Biosystems, 11(1): 116-122.
- Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW 1984. Ribosomal DNAsepacer-length polymorphism in barley: mendelian inheritance, chromosomal location, and population Dynamics. Proceedings of the National Academy Sciences, 81: 8014-8019.
- Sönmez İ 2017. Atık mantar kompostunun domates fidelerinin gelişimi ve besin içerikleri üzerine olan etkilerinin belirlenmesi. Mediterranean Agricultural Sciences, 30(1): 59-63.
- Sunar S, Bulut H 2019. Buğday (Triticum aestivum L.) Tohumlarında Büyüme Düzenleyicisi 2,4-D Isooctylester Herbisitinin Meydana Getirdiği Retrotranspozon Hareketliliğinin Moleküler Yöntem ile Değerlendirilmesi. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 12(2): 585-594.
- Temel A, Gözükirmizi N 2013. Analysis of retrotransposition and DNA methylation in barley callus culture. Acta Biologica Hungarica, 64 (1): 86-95.
- Teto AA, Laubscher CP, Ndakidemi PA, Matimati I 2016. Paclobutrazol retards vegetative growth in hydroponically-cultured Leonotis leonurus (L.) R. Br. Lamiaceae for a multipurpose flowering potted plant. South African Journal of Botany, 106: 67-70.
- Wessler SR 2009. Eukaryotic transposable elements: teaching old genomes new tricks’’, http://www.plantbio. uga. edu/ wesslerlab/The_Implicit_Genome.pdf, Son Erişim Tarihi: 10.03.2017.
- Wicker T, Sabot F, Hua-Van A, Bennetzen J, Capy P, Chalhoub B, Flavell A, Leroy P, Michele M, Olivier P, E Paux, Phillip S, Alan HS 2007. A Unified Classification System for Eukaryotic Transposable Elements. Nature Genetics, 8: 973-982.
- Yigider E, Taspinar MS, Sigmaz B, Aydin M, Agar G 2016. Humic acids protective activity against manganese induced LTR (long terminal repeat) retrotransposon polymorphism and genomic instability effects in Zea mays. Plant Gene, 6: 13-17.
- Zandstra JW, Squire RC, Watt GJ 2007. Managing transplant size and advancing field maturity of fresh tomatoes and peppers. In Ontario vegetable crop research: University of Guelph Ridgetown Campus, pp. 1-16.
There are 29 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Structural Biology, Agricultural, Veterinary and Food Sciences |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Publication Date | October 31, 2021 |
| Submission Date | June 18, 2020 |
| Acceptance Date | January 25, 2021 |
| Published in Issue | Year 2021Volume: 24 Issue: 5 |
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Examination of morphological and molecular changes in tomato (Solanum lycopersicum L.) seedlings with the application of tebuconazole
Acta Scientiarum Polonorum Hortorum Cultus
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