Effect of Rosa pimpinellifolia Extract on Human Tumor Cells Proliferation

Elif Ayazoglu Demir; Selim Demir; Nihal Türkmen; İbrahim Turan

doi:10.18016/ksutarimdoga.vi.848137

Araştırma Makalesi

Rosa pimpinellifolia Ekstraktının İnsan Tümör Hücrelerinin Çoğalmasına Etkisi

Yıl 2021, Cilt: 24 Sayı: 6, 1170 - 1176, 31.12.2021

Elif Ayazoglu Demir , Selim Demir , Nihal Türkmen , İbrahim Turan

https://doi.org/10.18016/ksutarimdoga.vi.848137

Cited By: 2

Öz

Rosa cinsi Rosaceae familyasının bir üyesi olup, Avrupa, Asya, Orta Doğu ve Kuzey Amerika'da dağılım gösteren 100'den fazla tür içerir. Rosa türleri, soğuk algınlığı, enfeksiyonlar ve çeşitli iltihaplı hastalıkların geleneksel tedavisinde uzun süredir kullanılmaktadır. Çeşitli çalışmalar bu cinsin antioksidan, antidiyabetik, anti-inflamatuar, renoprotektif ve antikanser aktivitelerini bildirmesine rağmen, Rosa pimpinellifolia'nın insan kanser hücreleri üzerindeki sitotoksik etkisini araştıran bir çalışma bulunmamaktadır. Bu çalışmanın amacı, R. pimpinellifolia'nın antioksidan özelliklerini ve insan kolon (WiDr), karaciğer (HepG2) ve akciğer (A549) kanser hücreleri üzerindeki sitotoksik etkisini normal bir fibroblast (BJ) hücre hattına kıyasla değerlendirmektir. Ekstraktın antioksidan özellikleri kolorimetrik yöntemler kullanılarak belirlenirken, sitotoksik etkisi ise MTT testi kullanılarak değerlendirildi. Ekstraktın toplam fenolik içeriği ve indirgeme gücü değerleri g örnek başına sırasıyla 16.4±0.4 mg gallik asit eşdeğeri ve 34.3±2.4 mg troloks eşdeğeri olarak hesaplandı. Ekstrakt BJ hücrelerine kıyasla üç kanser hücresi hattı üzerinde doza bağımlı bir şekilde seçici sitotoksik etki sergilemesine rağmen, en yüksek seçicilik indeks değeri (2.7 kat) WiDr hücre hattı için elde edildi. Sonuç olarak, bu çalışma R. pimpinellifolia'nın kanser hücreleri üzerindeki sitotoksik etkisini gösteren ilk çalışmadır. Bu sitotoksik etkinin moleküler mekanizmasını göstermek ve in vivo çalışmaların yapılması gelecekteki klinik çalışmalar için yol gösterici olabilir.

Anahtar Kelimeler

Aktioksidan aktivite, Hücre kültürü, Kanser, Rosa pimpinellifolia, Sitotoksisite

Kaynakça

Abotaleb M, Liskova A, Kubatka P, Büsselberg D 2020. Therapeutic potential of plant phenolic acids in the treatment of cancer. Biomolecules 10(2): 221.
Aliyazicioglu R, Demir S, Badem M, Sener SO, Korkmaz N, Demir EA, Ozgen U, Karaoglu SA, Aliyazicioglu Y 2017. Antioxidant, antigenotoxic, antimicrobial activities and phytochemical analysis of Dianthus carmelitarum. Rec Nat Prod 11(3): 270-284.
Alnajjar AM, Elsiesy HA 2015. Natural products and hepatocellular carcinoma: A review. Hepatoma Res 1: 119-124.
Alp S, Ercisli S, Jurikova T, Cakir O, Gozlekci S 2016. Bioactive content of Rose hips of different wildly grown Rosa dumalis genotypes. Not Bot Horti Agrobo 44(2): 472-476.
Artun FT, Karagoz A, Ozcan G, Melikoglu G, Anil S, Kultur S, Sutlupinar N 2016. In vitro anticancer and cytotoxic activities of some plant extracts on HeLa and Vero cell lines. J BUON 21(3): 720-725.
Ayazoglu Demir E, Demir S, Aliyazicioglu Y, 2020. In vitro cytotoxic effect of ethanol and dimethyl sulfoxide on various human cell lines. KSU J Agric Nat 23(5): 1119-1124.
Berkoz M, Yilmaz FO, Hunt AO, Yildirim M, Allahverdiyev O, Aslan A 2019. Rosa canina L. ethanolic exract induces the antiproliferative and apoptosis potential in MCF-7 and MDA-MB-468 cell lines. Fresenius Environ Bull 28(5): 3718-3725.
Bhave A, Schulzova V, Chmelarova H, Mrnka L, Hajslova J 2017. Assessment of rosehips based on the content of their biologically active compounds. J Food Drug Anal 25: 681-690.
Cagle P, Idassi O, Carpenter J, Minor R, Goktepe I, Martin P 2012. Effect of rosehip (Rosa canina) extracts on human brain tumor cell proliferation and apoptosis. J Cancer Ther 3: 534-545.
Cosmulescu S, Trandafir I, Nour V 2017. Phenolic acids and flavonoids profiles of extracts from edible wild fruits and their antioxidant properties. Int J Food Prop 20(12): 3124-3134.
Dekker E, Tanis PJ, Vleugels JLA, Kasi PM, Wallace MB 2019. Colorectal cancer. Lancet 394: 1467-1480.
Demir S, Turan I, Demir F, Ayazoglu Demir E, Aliyazicioglu Y 2017. Cytotoxic effect of Laurocerasus officinalis extract on human cancer cell lines. Marmara Pharm J 21: 121-126.
Demir S, Turan I, Aliyazicioglu R, Ozer Yaman S, Aliyazicioglu Y 2018a. Primula vulgaris extract induces cell cycle arrest and apoptosis in human cervix cancer cells. J Pharm Anal 8(5): 307-311.
Demir S, Turan I, Aliyazicioglu Y 2018b. Cytotoxic effect of Rhododendron luteum leaf extract on human cancer cell lines. KSU J Agric Nat 21(6): 950-956.
Demir S, Turan I, Aliyazicioglu Y 2019. Antioxidant properties of Primula vulgaris flower extract and its cytotoxic effect on human cancer cell lines. KSU J Agric Nat 22(1): 78-84.
de Oliveira PF, Damasceno JL, Bertanha CS, Araújo AR, Pauletti PM, Tavares DC 2016. Study of the cytotoxic activity of Styrax camporum extract and its chemical markers, egonol and homoegonol. Cytotechnology 68(4): 1597-1602.
Erguven M, Kultur S, Ozsoy N 2015. Anti-proliferative effect of Rosa agrestis on endometrium cancer cells in vitro. IJFER 1(1): 17-23.
Faezizadeh Z, Gharib A, Godarzee M 2016. Anti-proliferative and apoptotic effects of beta-ionone in human leukemia cell line K562. Zahedan J Res Med Sci 18(6): e7364.
Fattahi S, Jamei R, Hosseini Sarghein S 2012. Antioxidant and antiradical activities of Rosa canina and Rosa pimpinellifolia fruits from West Azerbaijan. Iran J Plant Physiol 2(4): 523-529.
Fujii T, Ikeda K, Saito M, 2011. Inhibitory effect of rose hip (Rosa canina L.) on melanogenesis in mouse melanoma cells and on pigmentation in brown guinea pigs. Biosci Biotechnol Biochem 75: 489-495.
Ghazghazi H, Miguel MG, Hasnaoui B, Sebei H, Ksontini M, Figueiredo AC, Pedro LG, Barroso JG 2010. Phenols, essential oils and carotenoids of Rosa canina from Tunisia and their antioxidant activities. Afr J Biotechnol 9(18): 2709-2716.
Guimarães R, Barros L, Calhelha RC, Carvalho AM, Queiroz MJRP, Ferreira ICFR 2014. Bioactivity of different enriched phenolic extracts of wild fruits from Northeastern Portugal: A comparative study. Plant Foods Hum Nutr 69: 37-42.
Jablonska-Rys E, Zalewska-Korona M, Kalbarczyk J 2009. Antioxidant capacity, ascorbic acid and phenolics content in wild edible fruits. J Fruit Ornam Plant Res 17(2): 115-120.
Jiménez S, Gascón S, Luquin A, Laguna M, Ancin-Azpilicueta C, Rodríguez-Yoldi MJ 2016. Rosa canina extracts have antiproliferative and antioxidant effects on Caco-2 human colon cancer. PLoS One 11(7): e0159136.
Khatib H, Rezaei-Tavirani M, Keshel SH, Azodi MZ, Omidi R, Biglarian M, Sobhi S 2013. Flow cytometry analysis of Rosa damascena effects on gastric cancer cell line (MKN45). Iran J Cancer Prev 6: 30-36.
Khazaei M, Khazaei MR, Pazhouhi M 2020. An overview of therapeutic potentials of Rosa canina: A traditionally valuable herb. WCRJ 7: e1580.
Kilinc K, Demir S, Turan I, Mentese A, Orem A, Sonmez M, Aliyazicioglu Y 2020. Rosa canina extract has antiproliferative and proapoptotic effects on human lung and prostate cancer cells. Nutr Cancer 72(2): 273-282.
Kim HH, Park KH, Kim MH, Oh MH, Kim SR, Park KJ, Heo JH, Lee MW 2013. Antiproliferative effects of native plants on prostate cancer cells. Nat Prod Sci 19(2): 192-200.
Koczka N, Stefanovits-Bányai E, Ombódi A 2018. Total polyphenol content and antioxidant capacity of rosehips of some Rosa species. Medicines 5: 84.
Lall RK, Syed DN, Adhami VM, Khan MI, Mukhtar H 2015. Dietary polyphenols in prevention and treatment of prostate cancer. Int J Mol Sci 16: 3350-3376.
Lee YH, Jung MG, Kang HB, Choi KC, Haam S, Jun W, Kim YJ, Cho HY, Yoon HG 2008. Effect of anti-histone acetyltransferase activity from Rosa rugosa Thunb. (Rosaceae) extracts on androgen receptor-mediated transcriptional regulation. J Ethnopharmacol 118: 412-417.
Liu W, Li SY, Huang XE, Cui JJ, Zhao T, Zhang H 2012. Inhibition of tumor growth in vitro by a combination of extracts from Rosa roxburghii Tratt and Fagopyrum cymosum. Asian Pac J Cancer Prev 13: 2409-2414.
Mármol I, Jiménez-Moreno N, Ancín-Azpilicueta C, Osada J, Cerrada E, Rodríguez-Yoldi MJ 2020. A combination of Rosa Canina extracts and gold complex favors apoptosis of Caco-2 cells by increasing oxidative stress and mitochondrial dysfunction. Antioxidants 9: 17.
Mavi A, Terzi Z, Ozgen U, Yildirim A, Coşkun M 2004. Antioxidant properties of some medicinal plants: Prangos ferulacea (Apiaceae), Sedum sempervivoides (Crassulaceae), Malva neglecta (Malvaceae), Cruciata taurica (Rubiaceae), Rosa pimpinellifolia (Rosaceae), Galium verum subsp. verum (Rubiaceae), Urtica dioica (Urticaceae). Biol Pharm Bull 27(5): 702-705.
Misir S, Aliyazicioglu Y, Demir S, Turan I, Ozer Yaman S, Deger O 2018. Antioxidant properties and protective effect of Turkish propolis on t-BHP-induced oxidative stress in foreskin fibroblast cells. IJPER 52(1): 94-100.
Misir S, Aliyazicioglu Y, Demir S, Turan I, Hepokur C 2020. Effect of Turkish propolis on miRNA expression, cell cycle, and apoptosis in human breast cancer (MCF-7) cells. Nutr Cancer 72(1):133-145.
Moreno MI, Isla MI, Sampietro AR, Vattuone MA 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 71(1-2): 109-114.
Mosmann T 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55-63.
Nađpal JD, Lesjak MM, Šibul FS, Anačkov GT, Četojević-Simin DD, Mimica-Dukić NM, Beara IN 2016. Comparative study of biological activities and phytochemical composition of two rose hips and their preserves: Rosa canina L. and Rosa arvensis Huds. Food Chem 192: 907-914.
Olech M, Nowak R, Los R, Rzymowska J, Malm A, Chrusciel K 2012. Biological activity and composition of teas and tinctures prepared from Rosa rugosa Thunb. Cent Eur J Biol 7(1): 172-182.
Olsson ME, Gustavsson KE, Andersson S, Nilsson A, Duan RD 2004. Inhibition of cancer cell proliferation in vitro by fruit and berry extracts and correlations with antioxidant levels. J Agric Food Chem 52: 7264-7271.
Oyaizu M 1986. Studies on product of browning reaction prepared from glucose amine. Jpn J Nutr 44: 307-315.
Ozkan MT, Aliyazicioglu R, Demir S, Misir S, Turan I, Yildirmis S, Aliyazicioglu Y 2017. Phenolic characterisation and antioxidant activity of Primula vulgaris and its antigenotoxic effect on fibroblast cells. Jundishapur J Nat Pharm Prod 12(1): e40073.
Paunović D, Kalušević A, Petrović T, Urošević T, Djinović D, Nedović V, Popović-Djordjević J 2019. Assessment of chemical and antioxidant properties of fresh and dried rosehip (Rosa canina L.). Not Bot Horti Agrobo 47(1): 108-113.
Radovanovic AM, Cupara SM, Popovic SLJ, Tomovic MT, Slavkovska VN, Jankovic SM 2013. Cytotoxic effect of Potentilla reptans L. rhizome and aerial part extracts. Acta Pol Pharm 70(5): 851-854.
Roman I, Stănilă A, Stănilă S 2013. Bioactive compounds and antioxidant activity of Rosa canina L. biotypes from spontaneous flora of Transylvania. Chem Cent J 7: 73.
Slinkard K, Singleton VL 1977. Total phenol analysis: automation and comparison with manual methods. Am J Enol Viticult 28: 49-55.
Tumbas VT, Canadanovic-Brunet JM, Cetojevic-Simin DD, Cetkovic GS, Dilas SM, Gille L 2012. Effect of rosehip (Rosa canina L.) phytochemicals on stable free radicals and human cancer cells. J Sci Food Agric 92: 1273-1281.
Turan I, Demir S, Aliyazicioglu R, Aliyazicioglu Y 2017. Evaluation of antioxidant and cytotoxic properties of Primula vulgaris leaf extract. KSU J Nat Sci 20(4): 361-367.
Turan I, Demir S, Kilinc K, Yaman SO, Misir S, Kara H, Genc B, Mentese A, Aliyazicioglu Y, Deger O 2018. Cytotoxic effect of Rosa canina extract on human colon cancer cells through repression of telomerase expression. J Pharm Anal 8(6): 394-399.
Tusat M, Mentese A, Demir S, Alver A, Imamoglu M 2017. Medical ozone therapy reduces oxidative stress and testicular damage in an experimental model of testicular torsion in rats. Braz J Urol 43(6):1160-1166.
Wadowska K, Bil-Lula I, Trembecki Ł, Sliwinska-Mosson M 2020. Genetic markers in lung cancer diagnosis: A review. Int J Mol Sci 21: 4569.
Zamiri-Akhlaghi A, Rakhshandeh H, Tayarani-Najaran Z, Mousavi SH 2011. Study of cytotoxic properties of Rosa damascena extract in human cervix carcinoma cell line. Avicenna J Phytomed 1(2): 74-77.
Zarei O, Yaghoobi MM 2019. Cytotoxic and anti-proliferative effects of Rosa beggeriana Schrenk extracts on human liver and breast cancer cells. Avicenna J Phytomed 9(4): 386-395.
Zhamanbayeva GT, Aralbayeva AN, Murzakhmetova MK, Tuleukhanov ST, Danilenko M 2016. Cooperative antiproliferative and differentiation-enhancing activity of medicinal plant extracts in acute myeloid leukemia cells. Biomed Pharmacother 82: 80-89.
Zhou Y, Zheng J, Li Y, Xu DP, Li S, Chen YM, Li HB 2016. Natural polyphenols for prevention and treatment of cancer. Nutrients 8: 515.

Effect of Rosa pimpinellifolia Extract on Human Tumor Cells Proliferation

Yıl 2021, Cilt: 24 Sayı: 6, 1170 - 1176, 31.12.2021

Elif Ayazoglu Demir , Selim Demir , Nihal Türkmen , İbrahim Turan

https://doi.org/10.18016/ksutarimdoga.vi.848137

Cited By: 2

Öz

The genus Rosa belongs to the Rosaceae family and contains more than 100 species which are distributed in Europe, Asia, the Middle East and North America. Rosa species have long been used in traditional treatment of cold, infections and various inflammatory diseases. Although various studies have reported antioxidant, antidiabetic, anti-inflammatory, renoprotective and anticancer activities of this genus, no previous studies have investigated the cytotoxic effect of Rosa pimpinellifolia on human cancer cells. The aim of this study was to evaluate the antioxidant properties of R. pimpinellifolia and its cytotoxic effect against human colon (WiDr), liver (HepG2) and lung (A549) cancer cells compared with a normal fibroblast (BJ) cell line. The antioxidant properties of extract was determined using colorimetric methods, while the cytotoxic effect of extract was evaluated using MTT assay. The total phenolic content and reducing power values of extract were calculated as 16.4±0.4 mg gallic acid equivalent and 34.3±2.4 mg trolox equivalent per g sample, respectively. Although the extract exhibits selective cytotoxic effect on three cancer cell lines in a dose-dependent manner compared to BJ cells, the highest selectivity index value (2.7-fold) was obtained for the WiDr cell line. In conclusion, this is the first study to demonstrate the cytotoxic effect of R. pimpinellifolia on cancer cells. To demonstrate the molecular mechanism of this cytotoxic effect and to carry out in vivo studies may be a guide for future clinical studies.

Anahtar Kelimeler

Antioxidant activity, Cancer, Cell culture, Cytotoxicity, Rosa pimpinellifolia

Kaynakça

Abotaleb M, Liskova A, Kubatka P, Büsselberg D 2020. Therapeutic potential of plant phenolic acids in the treatment of cancer. Biomolecules 10(2): 221.
Aliyazicioglu R, Demir S, Badem M, Sener SO, Korkmaz N, Demir EA, Ozgen U, Karaoglu SA, Aliyazicioglu Y 2017. Antioxidant, antigenotoxic, antimicrobial activities and phytochemical analysis of Dianthus carmelitarum. Rec Nat Prod 11(3): 270-284.
Alnajjar AM, Elsiesy HA 2015. Natural products and hepatocellular carcinoma: A review. Hepatoma Res 1: 119-124.
Alp S, Ercisli S, Jurikova T, Cakir O, Gozlekci S 2016. Bioactive content of Rose hips of different wildly grown Rosa dumalis genotypes. Not Bot Horti Agrobo 44(2): 472-476.
Artun FT, Karagoz A, Ozcan G, Melikoglu G, Anil S, Kultur S, Sutlupinar N 2016. In vitro anticancer and cytotoxic activities of some plant extracts on HeLa and Vero cell lines. J BUON 21(3): 720-725.
Ayazoglu Demir E, Demir S, Aliyazicioglu Y, 2020. In vitro cytotoxic effect of ethanol and dimethyl sulfoxide on various human cell lines. KSU J Agric Nat 23(5): 1119-1124.
Berkoz M, Yilmaz FO, Hunt AO, Yildirim M, Allahverdiyev O, Aslan A 2019. Rosa canina L. ethanolic exract induces the antiproliferative and apoptosis potential in MCF-7 and MDA-MB-468 cell lines. Fresenius Environ Bull 28(5): 3718-3725.
Bhave A, Schulzova V, Chmelarova H, Mrnka L, Hajslova J 2017. Assessment of rosehips based on the content of their biologically active compounds. J Food Drug Anal 25: 681-690.
Cagle P, Idassi O, Carpenter J, Minor R, Goktepe I, Martin P 2012. Effect of rosehip (Rosa canina) extracts on human brain tumor cell proliferation and apoptosis. J Cancer Ther 3: 534-545.
Cosmulescu S, Trandafir I, Nour V 2017. Phenolic acids and flavonoids profiles of extracts from edible wild fruits and their antioxidant properties. Int J Food Prop 20(12): 3124-3134.
Dekker E, Tanis PJ, Vleugels JLA, Kasi PM, Wallace MB 2019. Colorectal cancer. Lancet 394: 1467-1480.
Demir S, Turan I, Demir F, Ayazoglu Demir E, Aliyazicioglu Y 2017. Cytotoxic effect of Laurocerasus officinalis extract on human cancer cell lines. Marmara Pharm J 21: 121-126.
Demir S, Turan I, Aliyazicioglu R, Ozer Yaman S, Aliyazicioglu Y 2018a. Primula vulgaris extract induces cell cycle arrest and apoptosis in human cervix cancer cells. J Pharm Anal 8(5): 307-311.
Demir S, Turan I, Aliyazicioglu Y 2018b. Cytotoxic effect of Rhododendron luteum leaf extract on human cancer cell lines. KSU J Agric Nat 21(6): 950-956.
Demir S, Turan I, Aliyazicioglu Y 2019. Antioxidant properties of Primula vulgaris flower extract and its cytotoxic effect on human cancer cell lines. KSU J Agric Nat 22(1): 78-84.
de Oliveira PF, Damasceno JL, Bertanha CS, Araújo AR, Pauletti PM, Tavares DC 2016. Study of the cytotoxic activity of Styrax camporum extract and its chemical markers, egonol and homoegonol. Cytotechnology 68(4): 1597-1602.
Erguven M, Kultur S, Ozsoy N 2015. Anti-proliferative effect of Rosa agrestis on endometrium cancer cells in vitro. IJFER 1(1): 17-23.
Faezizadeh Z, Gharib A, Godarzee M 2016. Anti-proliferative and apoptotic effects of beta-ionone in human leukemia cell line K562. Zahedan J Res Med Sci 18(6): e7364.
Fattahi S, Jamei R, Hosseini Sarghein S 2012. Antioxidant and antiradical activities of Rosa canina and Rosa pimpinellifolia fruits from West Azerbaijan. Iran J Plant Physiol 2(4): 523-529.
Fujii T, Ikeda K, Saito M, 2011. Inhibitory effect of rose hip (Rosa canina L.) on melanogenesis in mouse melanoma cells and on pigmentation in brown guinea pigs. Biosci Biotechnol Biochem 75: 489-495.
Ghazghazi H, Miguel MG, Hasnaoui B, Sebei H, Ksontini M, Figueiredo AC, Pedro LG, Barroso JG 2010. Phenols, essential oils and carotenoids of Rosa canina from Tunisia and their antioxidant activities. Afr J Biotechnol 9(18): 2709-2716.
Guimarães R, Barros L, Calhelha RC, Carvalho AM, Queiroz MJRP, Ferreira ICFR 2014. Bioactivity of different enriched phenolic extracts of wild fruits from Northeastern Portugal: A comparative study. Plant Foods Hum Nutr 69: 37-42.
Jablonska-Rys E, Zalewska-Korona M, Kalbarczyk J 2009. Antioxidant capacity, ascorbic acid and phenolics content in wild edible fruits. J Fruit Ornam Plant Res 17(2): 115-120.
Jiménez S, Gascón S, Luquin A, Laguna M, Ancin-Azpilicueta C, Rodríguez-Yoldi MJ 2016. Rosa canina extracts have antiproliferative and antioxidant effects on Caco-2 human colon cancer. PLoS One 11(7): e0159136.
Khatib H, Rezaei-Tavirani M, Keshel SH, Azodi MZ, Omidi R, Biglarian M, Sobhi S 2013. Flow cytometry analysis of Rosa damascena effects on gastric cancer cell line (MKN45). Iran J Cancer Prev 6: 30-36.
Khazaei M, Khazaei MR, Pazhouhi M 2020. An overview of therapeutic potentials of Rosa canina: A traditionally valuable herb. WCRJ 7: e1580.
Kilinc K, Demir S, Turan I, Mentese A, Orem A, Sonmez M, Aliyazicioglu Y 2020. Rosa canina extract has antiproliferative and proapoptotic effects on human lung and prostate cancer cells. Nutr Cancer 72(2): 273-282.
Kim HH, Park KH, Kim MH, Oh MH, Kim SR, Park KJ, Heo JH, Lee MW 2013. Antiproliferative effects of native plants on prostate cancer cells. Nat Prod Sci 19(2): 192-200.
Koczka N, Stefanovits-Bányai E, Ombódi A 2018. Total polyphenol content and antioxidant capacity of rosehips of some Rosa species. Medicines 5: 84.
Lall RK, Syed DN, Adhami VM, Khan MI, Mukhtar H 2015. Dietary polyphenols in prevention and treatment of prostate cancer. Int J Mol Sci 16: 3350-3376.
Lee YH, Jung MG, Kang HB, Choi KC, Haam S, Jun W, Kim YJ, Cho HY, Yoon HG 2008. Effect of anti-histone acetyltransferase activity from Rosa rugosa Thunb. (Rosaceae) extracts on androgen receptor-mediated transcriptional regulation. J Ethnopharmacol 118: 412-417.
Liu W, Li SY, Huang XE, Cui JJ, Zhao T, Zhang H 2012. Inhibition of tumor growth in vitro by a combination of extracts from Rosa roxburghii Tratt and Fagopyrum cymosum. Asian Pac J Cancer Prev 13: 2409-2414.
Mármol I, Jiménez-Moreno N, Ancín-Azpilicueta C, Osada J, Cerrada E, Rodríguez-Yoldi MJ 2020. A combination of Rosa Canina extracts and gold complex favors apoptosis of Caco-2 cells by increasing oxidative stress and mitochondrial dysfunction. Antioxidants 9: 17.
Mavi A, Terzi Z, Ozgen U, Yildirim A, Coşkun M 2004. Antioxidant properties of some medicinal plants: Prangos ferulacea (Apiaceae), Sedum sempervivoides (Crassulaceae), Malva neglecta (Malvaceae), Cruciata taurica (Rubiaceae), Rosa pimpinellifolia (Rosaceae), Galium verum subsp. verum (Rubiaceae), Urtica dioica (Urticaceae). Biol Pharm Bull 27(5): 702-705.
Misir S, Aliyazicioglu Y, Demir S, Turan I, Ozer Yaman S, Deger O 2018. Antioxidant properties and protective effect of Turkish propolis on t-BHP-induced oxidative stress in foreskin fibroblast cells. IJPER 52(1): 94-100.
Misir S, Aliyazicioglu Y, Demir S, Turan I, Hepokur C 2020. Effect of Turkish propolis on miRNA expression, cell cycle, and apoptosis in human breast cancer (MCF-7) cells. Nutr Cancer 72(1):133-145.
Moreno MI, Isla MI, Sampietro AR, Vattuone MA 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 71(1-2): 109-114.
Mosmann T 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55-63.
Nađpal JD, Lesjak MM, Šibul FS, Anačkov GT, Četojević-Simin DD, Mimica-Dukić NM, Beara IN 2016. Comparative study of biological activities and phytochemical composition of two rose hips and their preserves: Rosa canina L. and Rosa arvensis Huds. Food Chem 192: 907-914.
Olech M, Nowak R, Los R, Rzymowska J, Malm A, Chrusciel K 2012. Biological activity and composition of teas and tinctures prepared from Rosa rugosa Thunb. Cent Eur J Biol 7(1): 172-182.
Olsson ME, Gustavsson KE, Andersson S, Nilsson A, Duan RD 2004. Inhibition of cancer cell proliferation in vitro by fruit and berry extracts and correlations with antioxidant levels. J Agric Food Chem 52: 7264-7271.
Oyaizu M 1986. Studies on product of browning reaction prepared from glucose amine. Jpn J Nutr 44: 307-315.
Ozkan MT, Aliyazicioglu R, Demir S, Misir S, Turan I, Yildirmis S, Aliyazicioglu Y 2017. Phenolic characterisation and antioxidant activity of Primula vulgaris and its antigenotoxic effect on fibroblast cells. Jundishapur J Nat Pharm Prod 12(1): e40073.
Paunović D, Kalušević A, Petrović T, Urošević T, Djinović D, Nedović V, Popović-Djordjević J 2019. Assessment of chemical and antioxidant properties of fresh and dried rosehip (Rosa canina L.). Not Bot Horti Agrobo 47(1): 108-113.
Radovanovic AM, Cupara SM, Popovic SLJ, Tomovic MT, Slavkovska VN, Jankovic SM 2013. Cytotoxic effect of Potentilla reptans L. rhizome and aerial part extracts. Acta Pol Pharm 70(5): 851-854.
Roman I, Stănilă A, Stănilă S 2013. Bioactive compounds and antioxidant activity of Rosa canina L. biotypes from spontaneous flora of Transylvania. Chem Cent J 7: 73.
Slinkard K, Singleton VL 1977. Total phenol analysis: automation and comparison with manual methods. Am J Enol Viticult 28: 49-55.
Tumbas VT, Canadanovic-Brunet JM, Cetojevic-Simin DD, Cetkovic GS, Dilas SM, Gille L 2012. Effect of rosehip (Rosa canina L.) phytochemicals on stable free radicals and human cancer cells. J Sci Food Agric 92: 1273-1281.
Turan I, Demir S, Aliyazicioglu R, Aliyazicioglu Y 2017. Evaluation of antioxidant and cytotoxic properties of Primula vulgaris leaf extract. KSU J Nat Sci 20(4): 361-367.
Turan I, Demir S, Kilinc K, Yaman SO, Misir S, Kara H, Genc B, Mentese A, Aliyazicioglu Y, Deger O 2018. Cytotoxic effect of Rosa canina extract on human colon cancer cells through repression of telomerase expression. J Pharm Anal 8(6): 394-399.
Tusat M, Mentese A, Demir S, Alver A, Imamoglu M 2017. Medical ozone therapy reduces oxidative stress and testicular damage in an experimental model of testicular torsion in rats. Braz J Urol 43(6):1160-1166.
Wadowska K, Bil-Lula I, Trembecki Ł, Sliwinska-Mosson M 2020. Genetic markers in lung cancer diagnosis: A review. Int J Mol Sci 21: 4569.
Zamiri-Akhlaghi A, Rakhshandeh H, Tayarani-Najaran Z, Mousavi SH 2011. Study of cytotoxic properties of Rosa damascena extract in human cervix carcinoma cell line. Avicenna J Phytomed 1(2): 74-77.
Zarei O, Yaghoobi MM 2019. Cytotoxic and anti-proliferative effects of Rosa beggeriana Schrenk extracts on human liver and breast cancer cells. Avicenna J Phytomed 9(4): 386-395.
Zhamanbayeva GT, Aralbayeva AN, Murzakhmetova MK, Tuleukhanov ST, Danilenko M 2016. Cooperative antiproliferative and differentiation-enhancing activity of medicinal plant extracts in acute myeloid leukemia cells. Biomed Pharmacother 82: 80-89.
Zhou Y, Zheng J, Li Y, Xu DP, Li S, Chen YM, Li HB 2016. Natural polyphenols for prevention and treatment of cancer. Nutrients 8: 515.

Toplam 56 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	İngilizce
Konular	Yapısal Biyoloji
Bölüm	ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar	Elif Ayazoglu Demir 0000-0001-9027-7633 Selim Demir 0000-0002-1863-6280 Nihal Türkmen 0000-0002-8913-8692 İbrahim Turan 0000-0003-3400-5494
Yayımlanma Tarihi	31 Aralık 2021
Gönderilme Tarihi	28 Aralık 2020
Kabul Tarihi	25 Mart 2021
Yayımlandığı Sayı	Yıl 2021Cilt: 24 Sayı: 6

Kaynak Göster

APA	Ayazoglu Demir, E., Demir, S., Türkmen, N., Turan, İ. (2021). Effect of Rosa pimpinellifolia Extract on Human Tumor Cells Proliferation. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 24(6), 1170-1176. https://doi.org/10.18016/ksutarimdoga.vi.848137