Research Article
First report of Bursaphelenchus leoni Baujard, 1980 (Nematoda: Parasitaphelenchidae) from Pine Forests of Turkey
Abstract
Several surveys have been performed in Turkey in order to determine a possible presence of Bursaphelenchus xylophilus (Steiner and Buhrer, 1934), Nickle, 1970. As a result, several Bursaphelenchus species have been reported. During a study aimed to identify potential insect vectors of these Bursaphelenchus species using the trap tree method in the western region of Turkey, one Bursaphelenchus species was isolated from a wood chip sample of a Pinus brutia trap log located in Bergama town of the city of İzmir. According to morphological and molecular studies, the species was determined as Bursaphelenchus leoni Baujard, 1980. Morphological characteristics of the species matched well with the original description made by Baujard in 1980. 18S and 28S rRNA genes of B. leoni were sequenced and a phylogenetic tree was constructed that showed B. leoni was clustered with B. leoni in the NCBI database. This is the first report of B. leoni from Turkey. To determine presence of other known Bursaphelenchus species in Turkey, new studies should be performed.
Keywords
Supporting Institution
Düzce University
Project Number
2011.02.02.076
Thanks
The authors kindly thank to Dr. Helen Braasch for morphological identification and confirmation and to Dr. Qing Yu for isolation of DNA and sequencing and to Dr. Terrel W. Stamps for reading and editing the manuscript.
References
- Abelleira A, Picoaga A, Mansilla JP, Aguin O 2011. Detection of Bursaphelenchus xylophilus, causal agent of pine wilt disease on Pinus pinaster in northwestern Spain. Plant Disease, 95: 776-776.
- Akbulut S, Yuksel B, Cebeci HH, Baysal I, Serin M, Erdem M 2010. Investigation of the pinewood nematode, Bursaphelenchus xylophilus (Nematoda: Parasitaphelenchidae) and other Bursaphelenchus species with their vector insects in pine forests of Turkey and their pathogenicities. Project Report, the Scientific and Technological Council of Turkey, Project no, 107O088, Ankara, pp. 113.
- Akbulut S, Vieira P, Ryss A, Valadas V, Keten A, Mota M 2008a. Bursaphelenchus Fuchs, 1937 (Nematoda: Parasitaphelenchidae) species associated with Pinus species in northern Turkey. Helminthologia, 45: 89-95.
- Akbulut S, Elekcioğlu IH, Keten A 2008b. First record of Bursaphelenchus vallesianus Braasch, Schönfeld, Polomski, and Burgermeister in Turkey. Turkish Journal of Agriculture and Forestry, 32: 273–279.
- Akbulut S, Vieira P, Ryss R, Yuksel B, Keten A, Mota M, Valadas A 2006. Preliminary survey of the pinewood nematode in Turkey. EPPO Bulletin, 36: 538-542.
- Akbulut S, Braasch H, Baysal I, Brandstetter M, Burgermeister W 2007. Description of Bursaphelenchus anamurius sp. n. (Nematoda: Parasitaphelenchidae) from Pinus brutia in Turkey. Nematology, 9: 859–867.
- Ambrogioni L, Caroppo S 1998. Morphology and morphometrics of Italian populations of Bursaphelenchus species. Nematologia Mediterranea, 26: 97-116.
- Ambrogioni L, Cerchiarini G, Irdani T, Tossani N 1994. Indagine preliminare sulla diffusione di Bursaphelenchus spp. (Nematoda) in pinete italiane. Redia, 77: 273-278.
- Atalay I 1995. Effects of climatic changes on the vegetation in the Near East. Bulletin de la Sciete de Geographie Degypte, 68: 157-177.
- Atalay I, Efe R, Oztur M 2014. Ecology and classification of forests in Turkey. Procedia-Social and Behavioral Sciences, 120: 788-805.
- Baujard P 1980. Trois nouvelles especes de Bursaphelenchus (Nematoda: Tylenchida) et remarques sur le genre. Revue de Nematologie, 3: 167-177.
- Braasch H, Philis J 2002. New records of Bursaphelenchus spp. in Cyprus. Nematologia Mediterranea, 30: 55-57.
- Braasch H, Swart A, Tribe G, Burgermeister W 1998. First record of Bursaphelenchus leoni in South Africa and comparison with some other Bursaphelenchus spp. EPPO bulletin, 28: 211-216.
- Braasch H, Metge K, Burgermeister W 1999. Bursaphelenchus species (Nematoda: Parasitaphelenchidae) found in coniferous trees in Germany and their ITS-RFLP patterns. Nachrichtenblatt des Deutschen Pflanzenschutzdienstes, 51: 312-320.
- Braasch H, Burgermeister W, Gu J 2009. Revised intra-generic grouping of Bursaphelenchus Fuchs, 1937 (Nematoda:Aphelenchoididae). J. Nematode Morphol. Sys. 12: 65-88.
- Capella-Gutierrez S, Silla-Martinez JM Gabaldon T 2009. TrimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics, 25: 1972-1973.
- Caroppo S, Ambrogioni L, Cavalli M, Coniglio, D 1998. Occurrence of pinewood nematodes Bursaphelenchus and their possible vectors in Italy. Nematologia Mediterranea, 26: 87-92.
- Darriba DL, Taboada G, Doallo R, Posada D 2012. JModelTet 2: more models, new heuristics and high-performance computing. Nature Methods, 9: 772.
- Dayi M, Calin M, Akbulut S, Gu J, Schröder T, Vieira P, Braasch H 2014. Morphological and molecular characterisation of Bursaphelenchus andrassyi sp. n. (Nematoda: Aphelenchoididae) from Romania and Turkey. Nematology, 6: 207-218.
- D'Errico G, Carletti B, Schröder T, Mota M, Vieira P, Roversi PF 2015. An update on the occurrence of nematodes belonging to the genus Bursaphelenchus in the Mediterranean area. Forestry, 88: 509–520.
- Edgar CR 2004. MUSCLE: multiple sequence aligment with high accuracy and high throughput. Nucleic Acids Research, 32: 1792-1797.
- Escuer M, Arias M, Bello A 2002. Nematodes associated with coniferous woods in Spain. Nematology, 4: 265-265.
- Escuer M, Arias M, Bello A 2004. Occurrence of the genus Bursaphelenchus Fuchs, 1937 (Nematoda: Aphelenchida) in Spanish conifer forests. Nematology, 6: 155-156.
- Futai K 2013. Pine wood nematode, Bursaphelenchus xylophilus. Annual Review Phytopatology, 51: 61-83. Griffith R, Giblin-Davis RM, Koshy P, Sosamma VK 2005. Nematode parasites of coconut and other palms. (Plant Parasitic Nematodes in Subtropical and Tropical Agriculture, CABI Publishing, UK: Ed. Luc M, Sikora R, Bridge J) 493-527.
- Hooper DJ 1986. Extraction of Free Living Stages from Soil, 5-30. in J. F. Southey ed. Laboratory Methods for Work with Plant and Soil Nematodes. London, UK: Her Majesty’s Stationery Office.
- Kanzaki N, Giblin-Davis RM 2018. Diversity and Plant Pathogenicity of Bursaphelenchus and Related Nematodes in Relation to Their Vector Bionomics. Current Forestry, 4: 85-100.
- Li H, Gu J, Fang Y, Guo K, Schonfeld U, Braasch H 2020. Supplementary characterisation of Bursaphelenchus leoni Baujard, 1980 (Tylenchina:Aphelenchoididae) found in Pinus massoniana from Zhejiang Province, China, with proposal of B. borealis Krentchenko, 1980 as a junior synonym. Nematology, 22: 1061-1078.
- Mamiya Y, 1983. Pathology of the pine wilt disease caused by Bursaphelenchus xylophilus. Annual Review of Phytopathology, 21: 201-220.
- Michalopoulos-Skarmoutsos H, Skarmoutsos G, Kalapanida M, Karageorgos A 2004. Surveying and recording of nematodes of the genus Bursaphelenchus in conifer forests in Greece and pathogenicity of the most important species. Proceedings of an International Workshop 20-22 August 2001, Portugal.
- Mota M, Braasch H, Bravo MA, Penas A C , Burgermeister W, Metge K, Sousa E 1999. First report of Bursaphelenchus xylophilus in Portugal and in Europe. Nematology 1: 727-734.
- Page RD 1996. TreeView: an application to display phylogenetic trees on personel computers. Computer Applications in the Bioscience, 12: 357-358.
- Palmisano AM, Ambrogioni L 1994. Nematodi Aphelenchoidoidea associati con Pinus spp. in Italia. Redia, 77: 225-240.
- Philis J 1996. An outlook on the association of Bursaphelenchus leoni with wilting pines in Cyprus. Nematologia Mediterranea, 24: 221-225.
- Philis J, Braasch H 1996. Occurrence of Bursaphelenchus leoni (Nematoda, Aphelenchoididae) in Cyprus and its extraction from pine wood. Nematologia Mediterranea, 24: 119-123.
- Ryss A, Vieira P, Mota M, Kulinich O 2005. A synopsis of the genus Bursaphelenchus Fuchs, 1937 (Aphelenchida: Parasitaphelenchidae) with keys to species. Nematology, 7: 393-458.
- Sanger F, Nicklen S, Coulson AR 1977. DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U S A 74: 5463-7.
- Seinhorst JW 1959. A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica, 4: 67–69.
- Skarmoutsos G, Michalopoulos-Skarmoutsos H, 2000. Pathogenicity of Bursaphelenchus sexdentati, Bursaphelenchus leoni and Bursaphelenchus hellenicus on European pine seedlings. Forest Pathology, 30: 149-156.
- Skarmoutsos G, Skarmoutsos H 1999. First record of Bursaphelenchus nematodes from pine forests in Greece. Plant Diseases, 83: 879.4-879.4.
- Stamatakis A 2014. RAxML Version 8: A tool for Phylogenetic Analysis and Post-Analysis of Large Phylogenies. Bioinformatics, 30: 1312-1313.
- Tomiczek C 2000. A survey for Bursaphelenchus spp. in conifers in Austria and implications to Austria forests. 399. XXI IUFRO World Congress 2000 7-12 August 2000, Kuala Lumpur, Malaysia.
Bursaphelenchus leoni Baujard, 1980 (Tylenchina: Aphelenchoididae)'nin Türkiye'nin Çam Ormanlarından Ilk Kaydı
Abstract
Bursaphelenchus xylophilus (Steiner and Buhrer, 1934), Nickle, 1970’un tespiti için Türkiye’de bir kaç arazi calısmaları yapılmıştır. Sonuç olarak, bir kaç Bursaphelenchus türü rapor edilmiştir. Rapor edilen bu Bursaphelenchus türlerinin Türkiye’nin batı bölgesinde taşıyıcı böceklerinin tuzak ağaçları ile belirlenmesi icin yürütülen bir çalışmada, İzmir’in Bergama ilçesinde Kızılçam kütüklerinden bir Bursaphelenchus türü izole edilmistir. Morfolojik ve moleküler çalısmalara göre, bu tür Bursaphelenchus leoni Baujard, 1980 olarak belirlenmiştir. Bu türün morfolojik özellikleri 1980 yılında Baujard tarafından yapılan orjinal tanımlamayla eşleşmiştir. 18S and 28S rRNA genlerinin dizileyip filogenetik ağaç oluşturulmuş ve bu ağaç izole edilen B. leoni’nin NCBI veribankasındaki B.leoni türleri ile kümelendiğini göstermistir. Bu, B.leoni’nin Türkiye’den ilk kaydıdır. Bilinen diğer Bursaphelenchus türlerinin Türkiye’deki varlıklarının belirlenmesi için yeni çalısmalar yürütülmelidir.
Keywords
Project Number
2011.02.02.076
References
- Abelleira A, Picoaga A, Mansilla JP, Aguin O 2011. Detection of Bursaphelenchus xylophilus, causal agent of pine wilt disease on Pinus pinaster in northwestern Spain. Plant Disease, 95: 776-776.
- Akbulut S, Yuksel B, Cebeci HH, Baysal I, Serin M, Erdem M 2010. Investigation of the pinewood nematode, Bursaphelenchus xylophilus (Nematoda: Parasitaphelenchidae) and other Bursaphelenchus species with their vector insects in pine forests of Turkey and their pathogenicities. Project Report, the Scientific and Technological Council of Turkey, Project no, 107O088, Ankara, pp. 113.
- Akbulut S, Vieira P, Ryss A, Valadas V, Keten A, Mota M 2008a. Bursaphelenchus Fuchs, 1937 (Nematoda: Parasitaphelenchidae) species associated with Pinus species in northern Turkey. Helminthologia, 45: 89-95.
- Akbulut S, Elekcioğlu IH, Keten A 2008b. First record of Bursaphelenchus vallesianus Braasch, Schönfeld, Polomski, and Burgermeister in Turkey. Turkish Journal of Agriculture and Forestry, 32: 273–279.
- Akbulut S, Vieira P, Ryss R, Yuksel B, Keten A, Mota M, Valadas A 2006. Preliminary survey of the pinewood nematode in Turkey. EPPO Bulletin, 36: 538-542.
- Akbulut S, Braasch H, Baysal I, Brandstetter M, Burgermeister W 2007. Description of Bursaphelenchus anamurius sp. n. (Nematoda: Parasitaphelenchidae) from Pinus brutia in Turkey. Nematology, 9: 859–867.
- Ambrogioni L, Caroppo S 1998. Morphology and morphometrics of Italian populations of Bursaphelenchus species. Nematologia Mediterranea, 26: 97-116.
- Ambrogioni L, Cerchiarini G, Irdani T, Tossani N 1994. Indagine preliminare sulla diffusione di Bursaphelenchus spp. (Nematoda) in pinete italiane. Redia, 77: 273-278.
- Atalay I 1995. Effects of climatic changes on the vegetation in the Near East. Bulletin de la Sciete de Geographie Degypte, 68: 157-177.
- Atalay I, Efe R, Oztur M 2014. Ecology and classification of forests in Turkey. Procedia-Social and Behavioral Sciences, 120: 788-805.
- Baujard P 1980. Trois nouvelles especes de Bursaphelenchus (Nematoda: Tylenchida) et remarques sur le genre. Revue de Nematologie, 3: 167-177.
- Braasch H, Philis J 2002. New records of Bursaphelenchus spp. in Cyprus. Nematologia Mediterranea, 30: 55-57.
- Braasch H, Swart A, Tribe G, Burgermeister W 1998. First record of Bursaphelenchus leoni in South Africa and comparison with some other Bursaphelenchus spp. EPPO bulletin, 28: 211-216.
- Braasch H, Metge K, Burgermeister W 1999. Bursaphelenchus species (Nematoda: Parasitaphelenchidae) found in coniferous trees in Germany and their ITS-RFLP patterns. Nachrichtenblatt des Deutschen Pflanzenschutzdienstes, 51: 312-320.
- Braasch H, Burgermeister W, Gu J 2009. Revised intra-generic grouping of Bursaphelenchus Fuchs, 1937 (Nematoda:Aphelenchoididae). J. Nematode Morphol. Sys. 12: 65-88.
- Capella-Gutierrez S, Silla-Martinez JM Gabaldon T 2009. TrimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics, 25: 1972-1973.
- Caroppo S, Ambrogioni L, Cavalli M, Coniglio, D 1998. Occurrence of pinewood nematodes Bursaphelenchus and their possible vectors in Italy. Nematologia Mediterranea, 26: 87-92.
- Darriba DL, Taboada G, Doallo R, Posada D 2012. JModelTet 2: more models, new heuristics and high-performance computing. Nature Methods, 9: 772.
- Dayi M, Calin M, Akbulut S, Gu J, Schröder T, Vieira P, Braasch H 2014. Morphological and molecular characterisation of Bursaphelenchus andrassyi sp. n. (Nematoda: Aphelenchoididae) from Romania and Turkey. Nematology, 6: 207-218.
- D'Errico G, Carletti B, Schröder T, Mota M, Vieira P, Roversi PF 2015. An update on the occurrence of nematodes belonging to the genus Bursaphelenchus in the Mediterranean area. Forestry, 88: 509–520.
- Edgar CR 2004. MUSCLE: multiple sequence aligment with high accuracy and high throughput. Nucleic Acids Research, 32: 1792-1797.
- Escuer M, Arias M, Bello A 2002. Nematodes associated with coniferous woods in Spain. Nematology, 4: 265-265.
- Escuer M, Arias M, Bello A 2004. Occurrence of the genus Bursaphelenchus Fuchs, 1937 (Nematoda: Aphelenchida) in Spanish conifer forests. Nematology, 6: 155-156.
- Futai K 2013. Pine wood nematode, Bursaphelenchus xylophilus. Annual Review Phytopatology, 51: 61-83. Griffith R, Giblin-Davis RM, Koshy P, Sosamma VK 2005. Nematode parasites of coconut and other palms. (Plant Parasitic Nematodes in Subtropical and Tropical Agriculture, CABI Publishing, UK: Ed. Luc M, Sikora R, Bridge J) 493-527.
- Hooper DJ 1986. Extraction of Free Living Stages from Soil, 5-30. in J. F. Southey ed. Laboratory Methods for Work with Plant and Soil Nematodes. London, UK: Her Majesty’s Stationery Office.
- Kanzaki N, Giblin-Davis RM 2018. Diversity and Plant Pathogenicity of Bursaphelenchus and Related Nematodes in Relation to Their Vector Bionomics. Current Forestry, 4: 85-100.
- Li H, Gu J, Fang Y, Guo K, Schonfeld U, Braasch H 2020. Supplementary characterisation of Bursaphelenchus leoni Baujard, 1980 (Tylenchina:Aphelenchoididae) found in Pinus massoniana from Zhejiang Province, China, with proposal of B. borealis Krentchenko, 1980 as a junior synonym. Nematology, 22: 1061-1078.
- Mamiya Y, 1983. Pathology of the pine wilt disease caused by Bursaphelenchus xylophilus. Annual Review of Phytopathology, 21: 201-220.
- Michalopoulos-Skarmoutsos H, Skarmoutsos G, Kalapanida M, Karageorgos A 2004. Surveying and recording of nematodes of the genus Bursaphelenchus in conifer forests in Greece and pathogenicity of the most important species. Proceedings of an International Workshop 20-22 August 2001, Portugal.
- Mota M, Braasch H, Bravo MA, Penas A C , Burgermeister W, Metge K, Sousa E 1999. First report of Bursaphelenchus xylophilus in Portugal and in Europe. Nematology 1: 727-734.
- Page RD 1996. TreeView: an application to display phylogenetic trees on personel computers. Computer Applications in the Bioscience, 12: 357-358.
- Palmisano AM, Ambrogioni L 1994. Nematodi Aphelenchoidoidea associati con Pinus spp. in Italia. Redia, 77: 225-240.
- Philis J 1996. An outlook on the association of Bursaphelenchus leoni with wilting pines in Cyprus. Nematologia Mediterranea, 24: 221-225.
- Philis J, Braasch H 1996. Occurrence of Bursaphelenchus leoni (Nematoda, Aphelenchoididae) in Cyprus and its extraction from pine wood. Nematologia Mediterranea, 24: 119-123.
- Ryss A, Vieira P, Mota M, Kulinich O 2005. A synopsis of the genus Bursaphelenchus Fuchs, 1937 (Aphelenchida: Parasitaphelenchidae) with keys to species. Nematology, 7: 393-458.
- Sanger F, Nicklen S, Coulson AR 1977. DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U S A 74: 5463-7.
- Seinhorst JW 1959. A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica, 4: 67–69.
- Skarmoutsos G, Michalopoulos-Skarmoutsos H, 2000. Pathogenicity of Bursaphelenchus sexdentati, Bursaphelenchus leoni and Bursaphelenchus hellenicus on European pine seedlings. Forest Pathology, 30: 149-156.
- Skarmoutsos G, Skarmoutsos H 1999. First record of Bursaphelenchus nematodes from pine forests in Greece. Plant Diseases, 83: 879.4-879.4.
- Stamatakis A 2014. RAxML Version 8: A tool for Phylogenetic Analysis and Post-Analysis of Large Phylogenies. Bioinformatics, 30: 1312-1313.
- Tomiczek C 2000. A survey for Bursaphelenchus spp. in conifers in Austria and implications to Austria forests. 399. XXI IUFRO World Congress 2000 7-12 August 2000, Kuala Lumpur, Malaysia.
There are 41 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Structural Biology |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Project Number | 2011.02.02.076 |
| Publication Date | August 31, 2022 |
| Submission Date | May 24, 2021 |
| Acceptance Date | September 9, 2021 |
| Published in Issue | Year 2022Volume: 25 Issue: 4 |
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