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Inula aucheriana'nın Antikanser, Antioksidan, Antimikrobiyal ve Enzim İnhibitör Aktiviteleri

Year 2022, Volume: 25 Issue: 5, 946 - 954, 31.10.2022

Gülşen Güçlü , Merve Ergül , Esra Uçar , Nuraniye Eruygur , Mehmet Ataş , Hüseyin Aşkın Akpulat

https://doi.org/10.18016/ksutarimdoga.vi.985837
Cited By: 4

Abstract

Türkiye'de yaygın olarak görülen Inula aucheriana, Asteraceae familyasına ait bir bitkidir. Çeşitli hastalıkların tedavisinde kullanıldığı bilinen bu bitkinin farklı biyolojik özelliklerinin araştırıldığı bu çalışma ile bitkinin farmakolojik potansiyelinin ortaya çıkarılması amaçlanmaktadır. Yöntemler: I.aucheriana'nın %80 etanol ekstraktı hazırlanarak, kimyasal bileşimi (Q-TOF analizi ile), antioksidant özellikleri (spektrofotometrik analizi ile), enzim inhibitör aktiviteleri (Ellman’s yöntemi ile), antimikrobiyal aktivitesi (MIC konsantrasyon değeri ile) ve antikanser aktivitesi (IC50 analizi ile) nitel ve nicel yöntemlerkullanılarak araştırıldı. Bulgular: I.aucheriana'nın %80 etanol ekstraktının güçlü bir antioksidan, antikanser ve enzim inhibitörü olduğu tespit edildi. Kimyasal bileşim analizinde ektraktın ana bileşiği luteolin (%32.55) olarak belirlendi. I. aucheriana ekstraktının AChE ve BChE inhibisyonuna sahip olduğu ortaya konuldu. Ayrıca Inula özütünün, referans ilaç ile karşılaştırıldığında ɑ-glukosidaz açısından inhibitör etkiye sahip olduğu belirlendi. Bununla birlikte oldukça yüksek tirozinaz enzim inhibisyonu gösterdi. Antiproliferatif aktivitesi incelendiğinde, MDA-MB-231 hücre hattında oldukça aktif olduğu bulundu. Sonuç: Bu çalışma, I. aucheriana'nın enzim inhibitör aktivitesi ve antioksidan aktivitesinin ilk araştırmasıdır. Çalışmadan elde edilen verilere göre I. aucheriana'nın etken maddeleri ile daha detaylı çalışmalar yapılabilir ve farklı hastalıkların tedavisi için umut verici sonuçlar alınabilir.

Keywords

Inula aucheriana Antikanser Antioksidan Enzim inhibitörü Tıbbi ve Aromatik Bitkiler

References

  • Al-Fartosy AJM 2011. Antioxidant properties of methanolic extract from Inula graveolens L. Turkish J Agric For. 35(6):591–596.
  • Bai N, Zhou Z, Zhu N, Zhang L, Quan Z, He K, Zheng QY, Ho CT 2005. Antioxidative flavonoids from the flower of Inula britannica. J Food Lipids. 12(2):141–149.
  • Blois MS 1958. Antioxidant determinations by the use of a stable free radical [10]. Nature. 181(4617):1199–1200.
  • Čanadanović-Brunet JM, Đilas SM, Ćemković GS, Tumbas VT, Malešević Z N 2002. ESR studies of antioxidative activity of different elecampane (Inula helenium L) extracts. Acta periodica technologica. (33): 127-134.
  • Clarke G, Ting KN, Wiart C,Fry J 2013. Radical Scavenging, Ferric Reducing Activity Potential and Total Phenolics Content Indicates Redundancy in Use of All Three Assays to Screen for Antioxidant Activity of Extracts of Plants from the Malaysian Rainforest.Antioxidants. 4;2(1):1-10.
  • CLSI 2002. Reference Reference Method for Broth Dilution Antifungal Suscept- ibility Testing of Yeasts, Approved Standard, 2nd ed., NCCLS document M27- A2. CLSI, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087- 1898, USA.
  • CLSI 2012. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, Approved Standard, 9th ed., CLSI document M07-A9. Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087, USA.
  • Cui YQ, Liu YJ, Zhang F 2018. The suppressive effects of Britannin (Bri) on human liver cancer through inducing apoptosis and autophagy via AMPK activation regulated by ROS. Biochem Biophys Res Commun. 497(3):916–923.
  • Diguță C, Cornea CP, Ioniță L, Brîndușe E, Farcaș N, Bobit D, & Matei F 2014. Studies on antimicrobial activity of Inula helenium L Romanian cultivar. Romanian Biotechnological Letters.19(5):9699- 9704.
  • Ekbatan MR, Khoramjouy M, Gholamine B, Faizi M, Sahranavard S 2019. Evaluation of anticonvulsant effect of aqueous and methanolic extracts of seven Inula species. Iran J Pharm Res. 18(Special Issue):208–220.
  • Ellman GL, Courtney KD, Andres V, Featherstone RM 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 7(2):88–95.
  • Eloff JN 1998. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med. 64(8):711–713.
  • Ergül M, Ergül M, Eruygur N, Ataş M, Uçar E 2019. In vitro evaluation of the chemical composition and various biological activities of ficus carica leaf extracts. Turkish J Pharm Sci. 16(4):401–409.
  • Gohari AR, Mosaddegh M, Naghibi F, Eslami-Tehrani B, Pirani A, Hamzeloo-Moghadam M, Read RW 2015. Cytotoxic sesquiterpene lactones from the aerial parts of Inula aucheriana. An Acad Bras Cienc. 87(2):777–785.
  • Gökbulut A, Özhan O, Satilmis B, Batçioglu K, Günal S, Şarer E 2013. Antioxidant and antimicrobial activities, and phenolic compounds of selected inula species from Turkey. Nat Prod Commun. 8(4):475–478.
  • Gupta RK, Patel AK, Shah N, Chaudhary AK, Jha UK, Yadav UC, Gupta PK, Pakuwal U 2014. Oxidative stress and antioxidants in disease and cancer: A review. Asian Pacific J Cancer Prev. 15(11):4405–4409.
  • Hashemi SM, Emami S 2015. Kojic acid-derived tyrosinase inhibitors: synthesis and bioactivity. Pharm Biomed Res. 1(1):1–17.
  • Hepokur C, Budak Y, Karayel HB, Selvi B,Yaylim İ 2019. Investigation of Cytotoxic Effects of Inula viscosa Extract. Cumhuriyet Science Journal.40(3):578-582.
  • Holetz FB, Pessini GL, Sanches NR, Cortez AG, Nakamura CV, Prado B, Filho D 2002. Screening Pl Medicinais 2.Pdf. Mem Inst Oswaldo Cruz. 97(10):1027–1031.
  • Jdey A, Falleh H, Jannet SB, Hammi KM, Dauvergne X, Ksouri R, Magné C 2017. Phytochemical investigation and antioxidant, antibacterial and anti-tyrosinase performances of six medicinal halophytes. South African Journal of Botany.112: 508-514.
  • Kaur R, KatariaD, Chahal KK 2014. Chemistry and biological activity of some alantoloids from inula species-a review. Pharmacophore.5(4): 536-551.
  • Khan AL, Hussain J, Hamayun M, Gilani SA, Ahmad S, Rehman G, Kim YH, Kang SM, Lee IJ 2010. Secondary metabolites from Inula britannica L. and their biological activities. Molecules. 15(3):1562–1577.
  • Kumar D, Gupta N, Ghosh R, Gaonkar RH, Pal BC 2013. α-Glucosidase and α-amylase inhibitory constituent of Carex baccans: Bio-assay guided isolation and quantification by validated RP-HPLC-DAD. J Funct Foods. 5(1):211–218.
  • Kumar D, Kumar H, Vedasiromoni JR, Pal BC 2012. Bio-assay guided isolation of a glucosidase inhibitory constituents from Hibiscus Mutabilis leaves. Phytochem Anal. 23(5):421–425.
  • Lin Y, Shi R, Wang X, Shen HM 2008. Luteolin, a flavonoid with potential for cancer prevention and therapy. Current cancer drug targets.8(7):634-646.
  • Mehndiratta M, Pandey S, Kuntzer T 2014. Acetylcholinesterase inhibitor treatment for myasthenia gravis ( Review ) Summary Of Findings For The Main Comparison. Cochrane database Syst Rev.(10).
  • Moghadam MH, Hajimehdipoor H, Saeidnia S, Atoofi A, Shahrestani R, Read RW, Mosaddegh M 2012. Anti-proliferative activity and apoptotic potential of britannin, a sesquiterpene lactone from Inula aucheriana. Nat Prod Commun. 7(8):979–980.
  • Molan AL, Mahdy AS 2014. Iraqi medicinal plants: Total flavonoid contents, free-radical scavenging and bacterial beta-glucuronidase inhibition activities. IOSR Journal of Dental and Medical Sciences. 13(5): 72-77.
  • Motor S, Ozturk S, Ozcan O, Gurpinar AB, Can Y, Yuksel R, Yenin JZ, Seraslan G, Ozturk OH 2014. Evaluation of total antioxidant status, total oxidant status and oxidative stress index in patients with alopecia areata. Int J Clin Exp Med. 7(4):1089–1093.
  • Orhan I, Şener B, Choudhary MI, Khalid A 2004. Acetylcholinesterase and butyrylcholinesterase inhibitory activity of some Turkish medicinal plants. J Ethnopharmacol. 91(1):57–60.
  • Orhan N, Gökbulut A,Orhan DD 2017. Antioxidant potential and carbohydrate digestive enzyme inhibitory effects of five Inula species and their major compounds. South African journal of botany.111: 86-92.
  • Ozkan E, Karakas FP, Yildirim AB, Tas I, Eker I, Yavuz MZ, Turker AU 2019. Promising medicinal plant Inula viscosa L.: Antiproliferative, antioxidant, antibacterial and phenolic profiles. Progress in Nutrition. 21(3):652-661.
  • Öztürk M, Çetin Ö 2013. Inula tuzgoluensis (Asteraceae), a new species from Central Anatolia, Turkey. Turk J Botany. 37(5):825–835.
  • Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-EvansC 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine. 26(9-10):1231-1237.
  • Trendafilova A, Ivanova V, Rangelov M, Todorova M, Ozek G, Yur S, Ozek T, Aneva I, Veleva R, Moskova-Doumanova V 2020. Caffeoylquinic Acids, Cytotoxic, Antioxidant, Acetylcholinesterase and Tyrosinase Enzyme Inhibitory Activities of Six Inula Species from Bulgaria. Chem Biodivers. 17(4).
  • Virdis P, Migheli R, Galleri G, Fancello S, Cadoni MPL, Pintore G, Petretto GL, Marchesi I, Fiorentino FP, di Francesco A 2020. Antiproliferative and proapoptotic effects of Inula viscosa extract on Burkitt lymphoma cell line. Tumor Biol. 42(2):1–9.
  • Wang Jiquan, Zhang Y, Liu X, Wang Jizhao, Li B, Liu Y, Wang Jiansheng 2019. Alantolactone enhances gemcitabine sensitivity of lung cancer cells through the reactive oxygen species-mediated endoplasmic reticulum stress and Akt/GSK3β pathway. Int J Mol Med. 44(3):1026–1038.
  • Zhao T, Ding KM, Zhang L, Cheng XM, Wang CH, Wang ZT 2013. Acetylcholinesterase and butyrylcholinesterase inhibitory activities of β -carboline and quinoline alkaloids derivatives from the plants of genus peganum. J Chem. 2013.

Anticancer, Antioxidant, Antimicrobial and Enzyme Inhibitory Activities of Inula aucheriana

Year 2022, Volume: 25 Issue: 5, 946 - 954, 31.10.2022

Gülşen Güçlü , Merve Ergül , Esra Uçar , Nuraniye Eruygur , Mehmet Ataş , Hüseyin Aşkın Akpulat

https://doi.org/10.18016/ksutarimdoga.vi.985837
Cited By: 4

Abstract

Inula aucheriana showing widespread in Turkey, it is a plant belonging to the Asteraceae family. It is aimed to reveal the pharmacological potential of this plant with this study, which investigates the different biological properties of this plant, which is known to be used in the treatment of various diseases. Methods: 80% ethanol extract of I.aucheriana was prepared and investigated the chemical composition (Q-TOF analysis), antioxidant (spectrophotometric analysis), enzyme inhibitory activities (Ellman's method), antimicrobial (MIC concentration value), anticancer (IC50 analysis) activities with qualitative and quantitative methods. Results: 80% ethanol extract of I.aucheriana was showed that is a powerful antioxidant, anticancer and enzyme inhibitor. In the chemical composition analysis, the major compound of the exctract was determined as luteolin (32.55%). I. aucheriana extract has been shown to have AChE and BChE inhibition. In addition, when the Inula extract was compared with the reference drug, it was determined that the extract had an inhibitory effect in terms of ɑ-glucosidase. It also showed quite high tyrosinase enzyme inhibition. When its antiproliferative activity was examined, it was found to be quite active in the MDA-MB-231 cell line. Conclusion: This study is the first investigation of the enzyme inhibitory activity and antioxidant activity of I. aucheriana. According to the data obtained from the study, more detailed studies can be conducted with the active ingredients of I. aucheriana, and promising results can be obtained for the treatment of different diseases.

Keywords

Inula aucheriana Anticancer Antioxidant Enzyme inhibitory Medicinal and Aromatic Plants

References

  • Al-Fartosy AJM 2011. Antioxidant properties of methanolic extract from Inula graveolens L. Turkish J Agric For. 35(6):591–596.
  • Bai N, Zhou Z, Zhu N, Zhang L, Quan Z, He K, Zheng QY, Ho CT 2005. Antioxidative flavonoids from the flower of Inula britannica. J Food Lipids. 12(2):141–149.
  • Blois MS 1958. Antioxidant determinations by the use of a stable free radical [10]. Nature. 181(4617):1199–1200.
  • Čanadanović-Brunet JM, Đilas SM, Ćemković GS, Tumbas VT, Malešević Z N 2002. ESR studies of antioxidative activity of different elecampane (Inula helenium L) extracts. Acta periodica technologica. (33): 127-134.
  • Clarke G, Ting KN, Wiart C,Fry J 2013. Radical Scavenging, Ferric Reducing Activity Potential and Total Phenolics Content Indicates Redundancy in Use of All Three Assays to Screen for Antioxidant Activity of Extracts of Plants from the Malaysian Rainforest.Antioxidants. 4;2(1):1-10.
  • CLSI 2002. Reference Reference Method for Broth Dilution Antifungal Suscept- ibility Testing of Yeasts, Approved Standard, 2nd ed., NCCLS document M27- A2. CLSI, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087- 1898, USA.
  • CLSI 2012. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, Approved Standard, 9th ed., CLSI document M07-A9. Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087, USA.
  • Cui YQ, Liu YJ, Zhang F 2018. The suppressive effects of Britannin (Bri) on human liver cancer through inducing apoptosis and autophagy via AMPK activation regulated by ROS. Biochem Biophys Res Commun. 497(3):916–923.
  • Diguță C, Cornea CP, Ioniță L, Brîndușe E, Farcaș N, Bobit D, & Matei F 2014. Studies on antimicrobial activity of Inula helenium L Romanian cultivar. Romanian Biotechnological Letters.19(5):9699- 9704.
  • Ekbatan MR, Khoramjouy M, Gholamine B, Faizi M, Sahranavard S 2019. Evaluation of anticonvulsant effect of aqueous and methanolic extracts of seven Inula species. Iran J Pharm Res. 18(Special Issue):208–220.
  • Ellman GL, Courtney KD, Andres V, Featherstone RM 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 7(2):88–95.
  • Eloff JN 1998. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med. 64(8):711–713.
  • Ergül M, Ergül M, Eruygur N, Ataş M, Uçar E 2019. In vitro evaluation of the chemical composition and various biological activities of ficus carica leaf extracts. Turkish J Pharm Sci. 16(4):401–409.
  • Gohari AR, Mosaddegh M, Naghibi F, Eslami-Tehrani B, Pirani A, Hamzeloo-Moghadam M, Read RW 2015. Cytotoxic sesquiterpene lactones from the aerial parts of Inula aucheriana. An Acad Bras Cienc. 87(2):777–785.
  • Gökbulut A, Özhan O, Satilmis B, Batçioglu K, Günal S, Şarer E 2013. Antioxidant and antimicrobial activities, and phenolic compounds of selected inula species from Turkey. Nat Prod Commun. 8(4):475–478.
  • Gupta RK, Patel AK, Shah N, Chaudhary AK, Jha UK, Yadav UC, Gupta PK, Pakuwal U 2014. Oxidative stress and antioxidants in disease and cancer: A review. Asian Pacific J Cancer Prev. 15(11):4405–4409.
  • Hashemi SM, Emami S 2015. Kojic acid-derived tyrosinase inhibitors: synthesis and bioactivity. Pharm Biomed Res. 1(1):1–17.
  • Hepokur C, Budak Y, Karayel HB, Selvi B,Yaylim İ 2019. Investigation of Cytotoxic Effects of Inula viscosa Extract. Cumhuriyet Science Journal.40(3):578-582.
  • Holetz FB, Pessini GL, Sanches NR, Cortez AG, Nakamura CV, Prado B, Filho D 2002. Screening Pl Medicinais 2.Pdf. Mem Inst Oswaldo Cruz. 97(10):1027–1031.
  • Jdey A, Falleh H, Jannet SB, Hammi KM, Dauvergne X, Ksouri R, Magné C 2017. Phytochemical investigation and antioxidant, antibacterial and anti-tyrosinase performances of six medicinal halophytes. South African Journal of Botany.112: 508-514.
  • Kaur R, KatariaD, Chahal KK 2014. Chemistry and biological activity of some alantoloids from inula species-a review. Pharmacophore.5(4): 536-551.
  • Khan AL, Hussain J, Hamayun M, Gilani SA, Ahmad S, Rehman G, Kim YH, Kang SM, Lee IJ 2010. Secondary metabolites from Inula britannica L. and their biological activities. Molecules. 15(3):1562–1577.
  • Kumar D, Gupta N, Ghosh R, Gaonkar RH, Pal BC 2013. α-Glucosidase and α-amylase inhibitory constituent of Carex baccans: Bio-assay guided isolation and quantification by validated RP-HPLC-DAD. J Funct Foods. 5(1):211–218.
  • Kumar D, Kumar H, Vedasiromoni JR, Pal BC 2012. Bio-assay guided isolation of a glucosidase inhibitory constituents from Hibiscus Mutabilis leaves. Phytochem Anal. 23(5):421–425.
  • Lin Y, Shi R, Wang X, Shen HM 2008. Luteolin, a flavonoid with potential for cancer prevention and therapy. Current cancer drug targets.8(7):634-646.
  • Mehndiratta M, Pandey S, Kuntzer T 2014. Acetylcholinesterase inhibitor treatment for myasthenia gravis ( Review ) Summary Of Findings For The Main Comparison. Cochrane database Syst Rev.(10).
  • Moghadam MH, Hajimehdipoor H, Saeidnia S, Atoofi A, Shahrestani R, Read RW, Mosaddegh M 2012. Anti-proliferative activity and apoptotic potential of britannin, a sesquiterpene lactone from Inula aucheriana. Nat Prod Commun. 7(8):979–980.
  • Molan AL, Mahdy AS 2014. Iraqi medicinal plants: Total flavonoid contents, free-radical scavenging and bacterial beta-glucuronidase inhibition activities. IOSR Journal of Dental and Medical Sciences. 13(5): 72-77.
  • Motor S, Ozturk S, Ozcan O, Gurpinar AB, Can Y, Yuksel R, Yenin JZ, Seraslan G, Ozturk OH 2014. Evaluation of total antioxidant status, total oxidant status and oxidative stress index in patients with alopecia areata. Int J Clin Exp Med. 7(4):1089–1093.
  • Orhan I, Şener B, Choudhary MI, Khalid A 2004. Acetylcholinesterase and butyrylcholinesterase inhibitory activity of some Turkish medicinal plants. J Ethnopharmacol. 91(1):57–60.
  • Orhan N, Gökbulut A,Orhan DD 2017. Antioxidant potential and carbohydrate digestive enzyme inhibitory effects of five Inula species and their major compounds. South African journal of botany.111: 86-92.
  • Ozkan E, Karakas FP, Yildirim AB, Tas I, Eker I, Yavuz MZ, Turker AU 2019. Promising medicinal plant Inula viscosa L.: Antiproliferative, antioxidant, antibacterial and phenolic profiles. Progress in Nutrition. 21(3):652-661.
  • Öztürk M, Çetin Ö 2013. Inula tuzgoluensis (Asteraceae), a new species from Central Anatolia, Turkey. Turk J Botany. 37(5):825–835.
  • Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-EvansC 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine. 26(9-10):1231-1237.
  • Trendafilova A, Ivanova V, Rangelov M, Todorova M, Ozek G, Yur S, Ozek T, Aneva I, Veleva R, Moskova-Doumanova V 2020. Caffeoylquinic Acids, Cytotoxic, Antioxidant, Acetylcholinesterase and Tyrosinase Enzyme Inhibitory Activities of Six Inula Species from Bulgaria. Chem Biodivers. 17(4).
  • Virdis P, Migheli R, Galleri G, Fancello S, Cadoni MPL, Pintore G, Petretto GL, Marchesi I, Fiorentino FP, di Francesco A 2020. Antiproliferative and proapoptotic effects of Inula viscosa extract on Burkitt lymphoma cell line. Tumor Biol. 42(2):1–9.
  • Wang Jiquan, Zhang Y, Liu X, Wang Jizhao, Li B, Liu Y, Wang Jiansheng 2019. Alantolactone enhances gemcitabine sensitivity of lung cancer cells through the reactive oxygen species-mediated endoplasmic reticulum stress and Akt/GSK3β pathway. Int J Mol Med. 44(3):1026–1038.
  • Zhao T, Ding KM, Zhang L, Cheng XM, Wang CH, Wang ZT 2013. Acetylcholinesterase and butyrylcholinesterase inhibitory activities of β -carboline and quinoline alkaloids derivatives from the plants of genus peganum. J Chem. 2013.
There are 38 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section RESEARCH ARTICLE
Authors

Gülşen Güçlü 0000-0002-3599-213X

Merve Ergül 0000-0003-4661-8087

Esra Uçar 0000-0001-6327-4779

Nuraniye Eruygur 0000-0002-4674-7009

Mehmet Ataş 0000-0002-9425-0080

Hüseyin Aşkın Akpulat 0000-0001-8394-2746

Publication Date October 31, 2022
Submission Date August 22, 2021
Acceptance Date October 22, 2021
Published in Issue Year 2022Volume: 25 Issue: 5

Cite

APA Güçlü, G., Ergül, M., Uçar, E., Eruygur, N., et al. (2022). Anticancer, Antioxidant, Antimicrobial and Enzyme Inhibitory Activities of Inula aucheriana. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(5), 946-954. https://doi.org/10.18016/ksutarimdoga.vi.985837

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