Aphis fabae (Hemiptera: Aphididae) Mücadelesinde Laurus nobilis (Lauraceae) Yağlarının Etkinliğinin Belirlenmesi
Abstract
Bu çalışmada bakla (Vicia faba L.) bitkisi üzerinde bulunan Aphis fabae Scopoli ile mücadelede Laourus nobilis L. bitkisinden elde edilen uçucu ve sabit yağların kullanılabilirliği Çukurova Üniversitesi Biyoloji ve Bitki Koruma Bölümleri laboratuvarlarında 2016-2017 yıllarında kurulan denemelerle araştırılmıştır. Sabit yağlar afitler üzerinde oldukça etkili olmasına karşın fitotoksik etkilere sahiptir bu sebeple sonraki çalışmalarda uçucu yağlar tercih edilmiştir. Hekzan ve Gliserol monostreat ile hazırlanan uçucu yağ dozlarından, özellikle 100 µl ml-1 ve üzerindeki dozlar fitotoksiteye neden olmuştur. Sonuçlara göre hekzanın afit ölümlerine neden olduğu anlaşılmış ve çalışmaya hekzan kullanılmadan devam edilmiştir. Gliserol monostreat ile hazırlanan hekzansız uygulamaların etkileri önemli ve anlamlı bulunmuştur. Özellikle 25 ve 50 µl ml-1 dozlarında ölüm oranları ve Abbott testi değerleri yüksek bulunmuştur. Ayrıca gliserin monostreatın kontrol grubunda afit ölümlerine yol açtığı fark edildiğinden çalışmaya Tween80 kullanılarak devam edilmiş ve istatistiksel olarak 25 ve 50 µl ml-1 dozlarının sonuçları önemli bulunmasına karsın 50 µl ml-1 dozunda bitkilerde fitotoksite gözlemlenmiştir. Bu sonuçlara göre Tween80 ile hazırlanan 25 µl ml-1 uçucu yağ dozunun afitlerle mücadelede en uygun doz olduğu sonucuna varılmıştır.
Supporting Institution
Çukurova Üniversitesi Bilimsel Araştırma Projeleri Birimi
Project Number
FBA-2016-6182
Thanks
Laboratuvarını kullanımımıza açıp yazım aşamasında eleştirilerini aldığımız Prof. Dr. Rifat ULUSOY ve bitkisel ekstraksiyon metotları hakkında bilgilerini esirgemeyen Prof. Dr. Saliha Kırıcı' ya teşekkürlerimizi sunarız.
References
- Barla A, Topçu G, Öksüz S, Tümen G, Kingston DG 2007. Identification of cytotoxic sesquiterpenes from Laurus nobilis L. Food chemistry 104(4): 1478-1484.
- De Corato U, Maccioni O, Trupo M, Di Sanzo G 2010. Use of essential oil of Laurus nobilis obtained by means of a supercritical carbon dioxide technique against post harvest spoilage fungi.Crop Protection 29(2): 142-147.
- Fiorini C, Fouraste I, David B, Bessiere JM 1997. Composition of the Flower, Leaf and Stem Essential Oils from Laurus nobilis. Flavour and fragrance journal 12(2): 91-93.
- Güncan A, Durmuşoğlu E 2004. Bitkisel Kökenli Doğal İnsektisitler Üzerine Bir Değerlendirme. Hasad 233: 26-32.
- Jemâa J. MB, Tersim N, Toudert KT, Khouja ML 2012. Insecticidal activities of essential oils from leaves of Laurus nobilis L. from Tunisia, Algeria and Morocco, and comparative chemical composition. Journal of Stored Products Research 48: 97-104.
- IBM Corp (2011) Released 2011. IBM SPSS Statistics for Windows, Version 20.0. Armonk, NY
- Marzouki H, Khaldi, A, ChamLi R, Bouzid S, Piras A, Falconieri D, Marongiu, B 2009. Biological activity evaluation of the oils from Laurus nobilis of Tunisia and Algeria extracted by supercritical carbon dioxide. Natural product research 23(3): 230-237.
- Merghni A, Marzouki H, Hentati H, Aouni M, Mastouri M 2016. Antibacterial and antibiofilm activities of Laurus nobilis L. essential oil against Staphylococcus aureus strains associated with oral infections. Current Research in Translational Medicine 64(1): 29-34.
- Saim N, Meloan CE 1986. Compounds from leaves of bay (Laurus nobilis L.) as repellents for Tribolium castaneum (Herbst) when added to wheat flour. Journal of Stored Products Research 22(3): 141-144.
- Santamarina MP, Roselló J, Giménez S, Blázquez MA 2016. Commercial Laurus nobilis L. and Syzygium aromaticum L. Merr. & Perry essential oils against post-harvest phytopathogenic fungi on rice.LWT-Food Science and Technology65: 325-332.
- Völkl W, Stechmann DH 1998. Parasitism of the black bean aphid (Aphis fabae) by Lysiphlebus fabarum (Hym., Aphidiidae): the influence of host plant and habitat. Journal of Applied Entomology 122(1-5): 201-206.
- Yeo H 2000.Mycoinsecticides for aphid management: a biorational approachUniversity of Nottingham, Doctoral dissertation, 319 p.
Efficacy of the Laurus nobilis oils (Lauraceae) on Controlling of Aphis fabae (Hemiptera: Aphididae)
Abstract
In this study efficacy of essential and fixed oil of Laurus nobilis L., on controlling Aphis fabae Scopoli that grows on Vicia faba L. (Fabaceae) has been studied by experiments set up in the laboratories of Çukurova University Biology and Plant Protection Departments in 2016-2017. Fixed oils had previously been found to be effective on aphids according to death rate and Abbott test values, but they had also phytotoxic effects on plants. Therefore, the essential oils were preferred in the present study. In essential oil solution applications (prepared with hexane and glycerol monostearate), 100 µL mL-1 and overdoses caused significant phytotoxicity and according to the results hexane significantly caused the death of aphids, therefore, the study was continued without the use of hexane. The effects of hexane-free solutions prepared with glycerol monostearate were found statistically differences (p<0,05) between doses (5, 10, 25, 50 µl mL-1). Especially the death rate and Abbott test results were higher at 25 and 50 µL mL-1 doses; however, no statistically significant difference was found 25 and 50 µL mL-1 doses. In addition, it was noticed that glycerol monostearate caused the death of aphids in the control group. Therefore, Tween 80 was used instead of glycerol monostearate in the last applications. In applications that prepared by Tween80, 25 and 50 µL mL-1 doses were found statistically significant (p<0,05) when compared to other doses (5 and 10 µl mL-1)but 50 µL mL-1 dose had phytotoxic effects. According to the results of this study, it is concluded that 25 µL mL-1 essential oil dose prepared by Tween 80 is the most suitable dose on aphid control.
Project Number
FBA-2016-6182
References
- Barla A, Topçu G, Öksüz S, Tümen G, Kingston DG 2007. Identification of cytotoxic sesquiterpenes from Laurus nobilis L. Food chemistry 104(4): 1478-1484.
- De Corato U, Maccioni O, Trupo M, Di Sanzo G 2010. Use of essential oil of Laurus nobilis obtained by means of a supercritical carbon dioxide technique against post harvest spoilage fungi.Crop Protection 29(2): 142-147.
- Fiorini C, Fouraste I, David B, Bessiere JM 1997. Composition of the Flower, Leaf and Stem Essential Oils from Laurus nobilis. Flavour and fragrance journal 12(2): 91-93.
- Güncan A, Durmuşoğlu E 2004. Bitkisel Kökenli Doğal İnsektisitler Üzerine Bir Değerlendirme. Hasad 233: 26-32.
- Jemâa J. MB, Tersim N, Toudert KT, Khouja ML 2012. Insecticidal activities of essential oils from leaves of Laurus nobilis L. from Tunisia, Algeria and Morocco, and comparative chemical composition. Journal of Stored Products Research 48: 97-104.
- IBM Corp (2011) Released 2011. IBM SPSS Statistics for Windows, Version 20.0. Armonk, NY
- Marzouki H, Khaldi, A, ChamLi R, Bouzid S, Piras A, Falconieri D, Marongiu, B 2009. Biological activity evaluation of the oils from Laurus nobilis of Tunisia and Algeria extracted by supercritical carbon dioxide. Natural product research 23(3): 230-237.
- Merghni A, Marzouki H, Hentati H, Aouni M, Mastouri M 2016. Antibacterial and antibiofilm activities of Laurus nobilis L. essential oil against Staphylococcus aureus strains associated with oral infections. Current Research in Translational Medicine 64(1): 29-34.
- Saim N, Meloan CE 1986. Compounds from leaves of bay (Laurus nobilis L.) as repellents for Tribolium castaneum (Herbst) when added to wheat flour. Journal of Stored Products Research 22(3): 141-144.
- Santamarina MP, Roselló J, Giménez S, Blázquez MA 2016. Commercial Laurus nobilis L. and Syzygium aromaticum L. Merr. & Perry essential oils against post-harvest phytopathogenic fungi on rice.LWT-Food Science and Technology65: 325-332.
- Völkl W, Stechmann DH 1998. Parasitism of the black bean aphid (Aphis fabae) by Lysiphlebus fabarum (Hym., Aphidiidae): the influence of host plant and habitat. Journal of Applied Entomology 122(1-5): 201-206.
- Yeo H 2000.Mycoinsecticides for aphid management: a biorational approachUniversity of Nottingham, Doctoral dissertation, 319 p.
Details
| Primary Language | English |
|---|---|
| Subjects | Structural Biology, Agricultural, Veterinary and Food Sciences |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Project Number | FBA-2016-6182 |
| Publication Date | December 30, 2022 |
| Submission Date | January 22, 2021 |
| Acceptance Date | November 11, 2021 |
| Published in Issue | Year 2022Volume: 25 Issue: 6 |
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KSU Journal of Agriculture and Nature
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