Investigating In Vitro Genotoxic Effects of Sweetener Xylitol

Ece Avuloğlu Yılmaz; Deniz Yüzbaşıoğlu; Fatma Ünal

doi:10.18016/ksutarimdoga.vi.993825

EN TR

Investigating In Vitro Genotoxic Effects of Sweetener Xylitol

Abstract

Xylitol (XYL) is a sweetener used as a food additive in the food industry. In the present study, four different genotoxicity assays (chromosomal aberration=CA, sister chromatid exchange=SCE, cytokinesis-block micronucleus cytome=CBMN-Cyt, and comet assays) were conducted to assess the potential genotoxicity of XYL in human lymphocytes. Four concentrations (125, 250, 500, and 1000 μg mL-1) of XYL were applied to lymphocytes obtained from three healthy young donors. The frequency of CA was not significantly affected by 24-h administration of XYL (except 1000 μg mL-1 for the number of CAs). 48 h treatment of XYL increased the frequency of CAs and abnormal cells. However, this increase was significant at only two highest concentrations. XYL significantly increased SCE/cell rate at the two highest concentrations at both treatment times (24 and 48h). Similarly, the frequency of MN, Nuclear buds (NBUDs), and Nucleoplasmic bridges (NPBs) significantly increased by XYL only at the high concentrations. It raised the comet parameters at the two highest concentrations. These observations showed that XYL, only at high concentrations, may have a genotoxic effect on human lymphocytes in vitro. For this reason, it can be concluded that its use at low concentrations may not cause DNA or chromosomal damage.

Keywords

Tatlandırıcı Olan Xylitol'ün In Vitro Genotoksik Etkilerinin İncelenmesi

Öz

Xylitol (XYL), gıda endüstrisinde gıda katkı maddesi olarak kullanılan bir tatlandırıcıdır. Bu çalışmada, insan lenfositlerinde XYL'nin potansiyel genotoksisitesini değerlendirmek için dört farklı genotoksisite testi (kromozomal anormallik=KA, kardeş kromatid değişimi=KKD, sitokinez-blok mikronükleus sitom=CBMN-Cyt ve comet testleri) gerçekleştirilmiştir. Genç ve sağlıklı donörlerden elde edilen lenfositlere dört farklı konsantrasyonda (125, 250, 500 ve 1000 μg mL-1) XYL uygulanmıştır. KA frekansı, 24 saatlik XYL uygulamasından önemli ölçüde etkilenmemiştir (anormal hücre sayısı için 1000 μg mL-1 hariç). 48 saatlik XYL muamelesi, kromozomal anormalliklerin ve anormal hücrelerin sıklığını arttırmıştır. Ancak bu artış en yüksek iki konsantrasyonda anlamlıdır. XYL, tüm uygulama sürelerinde (24 ve 48 saat) en yüksek iki konsantrasyonda KKD/hücre oranını önemli ölçüde yükseltmiştir. Benzer şekilde, MN, Nükleer tomurcuklar (NBUD'ler) ve Nükleoplazmik köprülerin (NPB'ler) sıklığı, XYL’ün yüksek konsantrasyonlarında anlamlı artış göstermiştir. XYL comet parametrelerini en yüksek iki konsantrasyonda yükseltmiştir. Bu gözlemler, XYL'nin in vitro insan lenfositlerinde yalnızca yüksek konsantrasyonlarda genotoksik bir etkiye sahip olabileceğini göstermiştir. Bu nedenle düşük konsantrasyonlarda kullanımının DNA veya kromozomal hasara neden olmayabileceği düşünülmektedir.

Anahtar Kelimeler

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Ece Avuloğlu Yılmaz
0000-0002-5164-3431
Türkiye

Deniz Yüzbaşıoğlu ^*
0000-0003-2756-7712
Türkiye

Fatma Ünal
0000-0002-7468-6186
Türkiye

Publication Date

December 30, 2022

Submission Date

September 10, 2021

Acceptance Date

November 26, 2021

Published in Issue

Year 2022 Volume: 25 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.993825

IZ

https://izlik.org/JA95HL94UY

Cite

RIS / Bibtex

APA

Avuloğlu Yılmaz, E., Yüzbaşıoğlu, D., & Ünal, F. (2022). Investigating In Vitro Genotoxic Effects of Sweetener Xylitol. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(6), 1315-1325. https://doi.org/10.18016/ksutarimdoga.vi.993825

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