Research Article
Bazı Nanogümüş Partiküllü (AgNPs) Bitki Su Ekstraklarının Kök-Ur Nematodu, Meloidogyne incognita (Kofoid & White) Chitwood (Nematoda: Meloidogynidae)’ya Karşı İn vitro Koşullarda Etkinliğinin Belirlenmesi
Abstract
Bu çalışma, Kök-ur nematodları [Meloidogyne incognita (Kofoid & White) Chitwood (Nematoda: Meloidogynidae)] (KUN)’na karşı bazı nanogümüş partiküllü (AgNPs) bitki su ekstraktlarının nematisidal etkilerini araştırmak amacıyla yürütülmüştür. Bu amaçla, Brassica carinata L. (Brassicaceae), Moringa oleifera L. (Moringaceae), Euphorbia helioscopia L. (Euphorbiaceae), Lantana camara L. (Verbenaceae) ve Datura stramonium L. (Solanaceae) bitkilerinden elde edilen (su ve nanogümüş katkılı) bitkisel ekstraktların KUN’a karşı etkileri in vitro koşullarında araştırılmıştır. Denemelerde M. incognita saf kültüründen elde edilen 2. dönem larva (L2)’lara karşı ekstraktlar 5 farklı konsantrasyonda (%5, %10, %15, %20 ve %25; 25 ppm, 50 ppm, 100 ppm, 150 ppm ve 200 ppm) uygulanmıştır. M. oleifera ve L. camara’nın bitki su ekstraktlarının %25 konsantrasyonu (sırasıyla %65.80, %70.70) ve nanogümüş partiküllü ekstraktlarının 200 ppm konsantrasyonu (sırasıyla %95.10, %94.10) etkili bulunmuştur.
Supporting Institution
Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü (ANKARA)
Project Number
TAGEM/BSAD/A/21/A2/P4/2533
Thanks
Tokat Gaziosmanpaşa Üniversitesi (TOKAT)’nde yürütülen doktora tez çalışmasının bir bölümü olup Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü (ANKARA) tarafından TAGEM/BSAD/A/21/A2/P4/2533 nolu proje ile desteklenmiştir.
References
- Abbasi MW, Ahmed N, Zaki MJ, Shaukat SS 2008. Effect of Barleria acanthoides Vahl. on root-knot nematode infection and growth of infected okra and brinjal plants. Pakistan Journal of Botany 40: 2193–2198.
- Abbott WS 1925. A Method of Computing the Effectiveness of an Insecticide. Journal of Economic Entomology 18: 265–267.
- Ahmed S, Grainge M 1988. Handbook of Plants with Pest Control Properties. John Wiley ve Sons Limited, New York, pages 470.
- Ardakani AS 2013. Toxicity of silver, titanium and silicon nanoparticles on the root-knot nematode, Meloidogyne incognita and growth parameters of tomato. Nematology 15: 671–677.
- Cromwell WA, Yang J, Starr JL, Ki Jo Y 2014. Nematicidal effects of silver nanoparticles on root knot nematode in bermuda grass. Journal of Nematology 46: 261–266.
- Çetintaş R , Kara H, 2016. Arthrobacter (ROA) ve Kadife Çiçeği (Tagetes patula) Ekstraktlarının Meloidogyne incognita (Kofoid & White) Populasyonuna Karşı Etkinliği. KSÜ Doğa Bilimleri Dergisi 19: 221–226.
- Çetintas R, Yarba MM 2010. Nematicidal effects of five plant essential oils on the Southern root-knot nematode, Meloidogyne incognita. Journal of Animal and Veterinary Advances 9: 222–225.
- Dura O, Sönmez İ, Yıldırım KC 2016. Biberde (Capsicum annuum L.) Salisilik Asit Uygulamalarının Kök-ur Nematodu (Meloidogyne incognita)’na ve Bazı Büyüme Parametreleri Üzerine Etkileri. Bahçe 45: 31–39.
- Dura O, Tülek A, Sönmez İ, Kepenekci İ 2018. Effects of Silver Nanoparticles (AgNPs) Using Aqueous Extract of Lantana camara L. (Lamiales: Verbenaceae) Applications against Rice White-Tip Nematode [Aphelenchoides besseyi Christie (Nematoda: Aphelenchida)] under Laboratory Conditions. 5th International Symposium on Multidisciplinary Studies. Current Academic Studies in Agricultural Sciences, 187–193.
- Dura O, Tülek A, Sönmez İ, Erdoğuş FD, Yeşilayer A, Kepenekci İ 2019. Effects Of Silver Nanoparticle (AgNPs) Applications Prepared Using Lantana camara L. (Lamiales: Verbenaceae)’s Aqueous Extract On Wheat Gal Nematode [Anguina tritici Thorne, 1949 (Nematoda: Anguinidae)]. Plant Protection Bulletin 59: 49–53.
- Hussey RS, Barker KRA 1973. comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Disease Reporter 57: 1025–1028.
- Kalaiselvi D, Sundararaj P, Premasudha P, Hafez SL 2017. Nematicidal activity of green synthesized silver nanoparticles using plant extracts against root-knot nematode Meloidogyne incognita. International Journal of Nematology 27: 81–94.
- Kalaiselvi D, Mohankumar A, Shanmugam Nivitha S, Sundararaj P 2019. Green synthesis of silver nanoparticles using latex extract of Euphorbia tirucalli: A novel approach for the management of root knot nematode, Meloidogyne incognita. Crop Protection 117: 108–114.
- Kepenekci I 2012. Nematoloji (Bitki Paraziti ve Entomopatojen Nematodlar) [Genel Nematoloji (Cilt-I), Taksonomik Nematoloji (Cilt-II)]. Eğitim, Yayım ve Yayımlar Dairesi Başkanlığı, Tarım Bilim Serisi Yayın No:3 (2012/3), LIV+1155 sayfa.
- Kepenekci I, Saglam, HD 2015. Extracts of some indigenous plants affecting hatching and mortality in the root-knot nematode [Meloidogyne javanica (Treub) Chitwood]. Egyptian Journal of Biological Pest Control 25: 39–44.
- Khan H, Ahmad R, Akhtar AS, Mahmood A, Basit T, Niaz T 2000. Effect of inoculum density of Meloidogyne incognita and plant age on the severity of root-knot disease in tomato. International Journal of Agriculture & Biology, 2000: 360–363.
- Kumar A, Chisti Y, Banerjee U. 2013. Synthesis of metallic nanoparticles using plant extracts: Biotechnology Advances 31: 346–356.
- Luc M, Sikora RA, Bridge J 2005. Plant parasitic nematodes in subtropical and tropical agriculture. Wallingford, UK, CABI Publishing, 871 pp.
- Melakeberhan H 1997. Effect of temperature and nitrojen source on tomato genotypes responce to Meloidogyne incognita infection. Fundamental and Applied Nematology 20: 1–8.
- Mousa EM, Mahdy ME, Younis Dalia M 2011. Evaluation of some plant extracts to control root knot nematodes, Meloidogyne spp. on tomato plants. Egyptian Journal of Agronematology 10: 1–14.
- Nartop P 2017. Biyosentetik gümüş nanopartiküllerinin Pyracantha coccinea bitkisinin gövde eksplantlarının yüzey sterilizasyonunda kullanımı. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi 23: 759–761.
- Nassar AM 2016. Effectiveness of silver nano-particles of extracts of Urtica urens (Urticaceae) against root-knot nematode Meloidogyne incognita. Asian Journal of Nematology 5: 14–19.
- Öncüer C 2000. Tarımsal zararlılarla savaş yöntem ve ilaçları (4.Baskı). Adnan Menderes Üniversitesi Yayınları. No:13, 333 sayfa, Aydın.
- Prakash A, Rao J 1996. Botanical Pesticides in Agriculture. CRC Pres, Lewis Publishers, 461 pp.
- Sağlam Altınköy HD, Dura O, Kepenekci İ 2020. Determination of The Effectiveness of Nano Silver Additive Aqueous Extract of Moringa oleifera L. (Brassicales: Moringaceae) Against Root Lesion Nematode [Prateylenchus thornei Sher & Allen) Chitwood (Nematoda: Pratylenchidae)] Under Laboratory Conditions. Journal of Global Innovations in Agricultural and Social Sciences 8: 19–22.
- Sankar MV, Abideen S 2015. Pesticidal effect of green synthesized silver and lead nanoparticles using Avivennia marina against grain storage pest Sitophilus oryzae. International Journal of Nanomaterials and Biostructures 5: 32–39.
- Sharma H, Dhirta B, Shirkot P 2017. Evaluation of biogenic iron nano formulations to control Meloidogyne incognita in okra. International Journal of Chemical Studies 5: 1278–1284.
- Smith D, Onions AHS 1994. The Preservation and Maintenance of Living Fungi. CAB International Bakeham Lane Egham-England.122 p.
- Taha EH 2016. Nematicidal effects of silver nanoparticles on root-knot nematodes (Meloidogyne incognita) in laboratory and screenhouse. Mansoura Journal of Plant Protection and Pathology 7: 333–337.
- Thakur RJ, Dhirta B, Shirkot P. 2018. Studies on Nano Toxicity Effect of Gold Nanoparticles on M. incognita and Tomato Plants Growth and Development. Annals of Nanoscience and Nanotechnology 2: 1–7.
- Vivek R, Thangam R, Muthuchelian K, Gunasekaran P, Kaveri K, Kannan S 2012. Green biosynthesis of silver nanoparticles from Annona squamosa leaf extract and its in vitro cytotoxic effect on MCF-7 cells. Process Biochemistry 47: 2405–2410.
Determination of The Effectiveness of Some Plant Water Extracts whit Nanosilver Particulated (AgNPs) Against Root Knot Nematode, Meloidogyne incognita (Kofoid & White) Chitwood (Nematoda: Meloidogynidae) In Vitro Conditions
Abstract
This study was conducted to determine the nematicidal effects of some nano-silver particle (AgNPs) plant aqueous extracts against root-knot nematodes [Meloidogyne incognita (Kofoid & White) Chitwood (Nematoda: Meloidogynidae)] (RKN). For this purpose, the effects of the extracts (water and nano-silver added) of Brassica carinata L. (Brassicaceae), Moringa oleifera L. (Moringaceae), Euphorbia helioscopia L. (Euphorbiaceae), Lantana camara L. (Verbenaceae) and Datura stramonium L. (Solanaceae) against RKN were investigated in in vitro conditions. Extracts were applied in 5 different concentrations (5%, 10%, 15%, 20% and 25%; 25 ppm, 50 ppm, 100 ppm, 150 ppm and 200 ppm) against second stage larvae (J2) obtained from M. incognita pure culture in the experiments. The 25% concentration of the plant water extracts (65.80%, 70.70%, respectively) and the 200 ppm concentration of the extracts with nanosilver particles (95.10%, 94.10%, respectively) of M. oleifera and L. camara were found to be effective.
Project Number
TAGEM/BSAD/A/21/A2/P4/2533
References
- Abbasi MW, Ahmed N, Zaki MJ, Shaukat SS 2008. Effect of Barleria acanthoides Vahl. on root-knot nematode infection and growth of infected okra and brinjal plants. Pakistan Journal of Botany 40: 2193–2198.
- Abbott WS 1925. A Method of Computing the Effectiveness of an Insecticide. Journal of Economic Entomology 18: 265–267.
- Ahmed S, Grainge M 1988. Handbook of Plants with Pest Control Properties. John Wiley ve Sons Limited, New York, pages 470.
- Ardakani AS 2013. Toxicity of silver, titanium and silicon nanoparticles on the root-knot nematode, Meloidogyne incognita and growth parameters of tomato. Nematology 15: 671–677.
- Cromwell WA, Yang J, Starr JL, Ki Jo Y 2014. Nematicidal effects of silver nanoparticles on root knot nematode in bermuda grass. Journal of Nematology 46: 261–266.
- Çetintaş R , Kara H, 2016. Arthrobacter (ROA) ve Kadife Çiçeği (Tagetes patula) Ekstraktlarının Meloidogyne incognita (Kofoid & White) Populasyonuna Karşı Etkinliği. KSÜ Doğa Bilimleri Dergisi 19: 221–226.
- Çetintas R, Yarba MM 2010. Nematicidal effects of five plant essential oils on the Southern root-knot nematode, Meloidogyne incognita. Journal of Animal and Veterinary Advances 9: 222–225.
- Dura O, Sönmez İ, Yıldırım KC 2016. Biberde (Capsicum annuum L.) Salisilik Asit Uygulamalarının Kök-ur Nematodu (Meloidogyne incognita)’na ve Bazı Büyüme Parametreleri Üzerine Etkileri. Bahçe 45: 31–39.
- Dura O, Tülek A, Sönmez İ, Kepenekci İ 2018. Effects of Silver Nanoparticles (AgNPs) Using Aqueous Extract of Lantana camara L. (Lamiales: Verbenaceae) Applications against Rice White-Tip Nematode [Aphelenchoides besseyi Christie (Nematoda: Aphelenchida)] under Laboratory Conditions. 5th International Symposium on Multidisciplinary Studies. Current Academic Studies in Agricultural Sciences, 187–193.
- Dura O, Tülek A, Sönmez İ, Erdoğuş FD, Yeşilayer A, Kepenekci İ 2019. Effects Of Silver Nanoparticle (AgNPs) Applications Prepared Using Lantana camara L. (Lamiales: Verbenaceae)’s Aqueous Extract On Wheat Gal Nematode [Anguina tritici Thorne, 1949 (Nematoda: Anguinidae)]. Plant Protection Bulletin 59: 49–53.
- Hussey RS, Barker KRA 1973. comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Disease Reporter 57: 1025–1028.
- Kalaiselvi D, Sundararaj P, Premasudha P, Hafez SL 2017. Nematicidal activity of green synthesized silver nanoparticles using plant extracts against root-knot nematode Meloidogyne incognita. International Journal of Nematology 27: 81–94.
- Kalaiselvi D, Mohankumar A, Shanmugam Nivitha S, Sundararaj P 2019. Green synthesis of silver nanoparticles using latex extract of Euphorbia tirucalli: A novel approach for the management of root knot nematode, Meloidogyne incognita. Crop Protection 117: 108–114.
- Kepenekci I 2012. Nematoloji (Bitki Paraziti ve Entomopatojen Nematodlar) [Genel Nematoloji (Cilt-I), Taksonomik Nematoloji (Cilt-II)]. Eğitim, Yayım ve Yayımlar Dairesi Başkanlığı, Tarım Bilim Serisi Yayın No:3 (2012/3), LIV+1155 sayfa.
- Kepenekci I, Saglam, HD 2015. Extracts of some indigenous plants affecting hatching and mortality in the root-knot nematode [Meloidogyne javanica (Treub) Chitwood]. Egyptian Journal of Biological Pest Control 25: 39–44.
- Khan H, Ahmad R, Akhtar AS, Mahmood A, Basit T, Niaz T 2000. Effect of inoculum density of Meloidogyne incognita and plant age on the severity of root-knot disease in tomato. International Journal of Agriculture & Biology, 2000: 360–363.
- Kumar A, Chisti Y, Banerjee U. 2013. Synthesis of metallic nanoparticles using plant extracts: Biotechnology Advances 31: 346–356.
- Luc M, Sikora RA, Bridge J 2005. Plant parasitic nematodes in subtropical and tropical agriculture. Wallingford, UK, CABI Publishing, 871 pp.
- Melakeberhan H 1997. Effect of temperature and nitrojen source on tomato genotypes responce to Meloidogyne incognita infection. Fundamental and Applied Nematology 20: 1–8.
- Mousa EM, Mahdy ME, Younis Dalia M 2011. Evaluation of some plant extracts to control root knot nematodes, Meloidogyne spp. on tomato plants. Egyptian Journal of Agronematology 10: 1–14.
- Nartop P 2017. Biyosentetik gümüş nanopartiküllerinin Pyracantha coccinea bitkisinin gövde eksplantlarının yüzey sterilizasyonunda kullanımı. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi 23: 759–761.
- Nassar AM 2016. Effectiveness of silver nano-particles of extracts of Urtica urens (Urticaceae) against root-knot nematode Meloidogyne incognita. Asian Journal of Nematology 5: 14–19.
- Öncüer C 2000. Tarımsal zararlılarla savaş yöntem ve ilaçları (4.Baskı). Adnan Menderes Üniversitesi Yayınları. No:13, 333 sayfa, Aydın.
- Prakash A, Rao J 1996. Botanical Pesticides in Agriculture. CRC Pres, Lewis Publishers, 461 pp.
- Sağlam Altınköy HD, Dura O, Kepenekci İ 2020. Determination of The Effectiveness of Nano Silver Additive Aqueous Extract of Moringa oleifera L. (Brassicales: Moringaceae) Against Root Lesion Nematode [Prateylenchus thornei Sher & Allen) Chitwood (Nematoda: Pratylenchidae)] Under Laboratory Conditions. Journal of Global Innovations in Agricultural and Social Sciences 8: 19–22.
- Sankar MV, Abideen S 2015. Pesticidal effect of green synthesized silver and lead nanoparticles using Avivennia marina against grain storage pest Sitophilus oryzae. International Journal of Nanomaterials and Biostructures 5: 32–39.
- Sharma H, Dhirta B, Shirkot P 2017. Evaluation of biogenic iron nano formulations to control Meloidogyne incognita in okra. International Journal of Chemical Studies 5: 1278–1284.
- Smith D, Onions AHS 1994. The Preservation and Maintenance of Living Fungi. CAB International Bakeham Lane Egham-England.122 p.
- Taha EH 2016. Nematicidal effects of silver nanoparticles on root-knot nematodes (Meloidogyne incognita) in laboratory and screenhouse. Mansoura Journal of Plant Protection and Pathology 7: 333–337.
- Thakur RJ, Dhirta B, Shirkot P. 2018. Studies on Nano Toxicity Effect of Gold Nanoparticles on M. incognita and Tomato Plants Growth and Development. Annals of Nanoscience and Nanotechnology 2: 1–7.
- Vivek R, Thangam R, Muthuchelian K, Gunasekaran P, Kaveri K, Kannan S 2012. Green biosynthesis of silver nanoparticles from Annona squamosa leaf extract and its in vitro cytotoxic effect on MCF-7 cells. Process Biochemistry 47: 2405–2410.
There are 31 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Agricultural, Veterinary and Food Sciences |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Project Number | TAGEM/BSAD/A/21/A2/P4/2533 |
| Publication Date | December 30, 2022 |
| Submission Date | September 13, 2021 |
| Acceptance Date | December 15, 2021 |
| Published in Issue | Year 2022Volume: 25 Issue: 6 |
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