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Uygulama Sonrası Zararlı Bulaşmasından Depolanmış Buğdayın Korunması Bakımından Beauveria bassiana Etkinliğinin Kalıcılığı

Year 2022, Volume: 25 Issue: Ek Sayı 1, 143 - 148, 30.12.2022

Huriye Yasemin Korkmaz , Mehmet Kubilay Er

https://doi.org/10.18016/ksutarimdoga.vi.1018408

Abstract

Bu çalışma, üç yerel Beauveria bassiana izolatının spor etkinlik sürelerinin belirlenmesi amacıyla, yaygın olan depolanmış ürün zararlılarından Sitophilus oryzae L. (Coleoptera: Curculionidae) Rhyzopertha dominica F. (Col.: Bostrichidae) ve Oryzaephilus surinamensis L. (Col.: Silvanidae) erginlerine karşı uygulanarak yürütülmüştür. Biyolojik testler için buğday danelerine 1000 ppm (w/w) konsantrasyonunda B. bassiana sporu karıştırılmış ve 1, 15 ve 28 gün sonra 20’şer ergin salımı yapılmıştır. Sitophilus oryzae ile yürütülen testlerde 3 izolat için genel olarak; 7. ve 14. gün ölüm oranı başlangıçta %33.3-41.6 ve %68.3-76.6 olup 28 gün sonraki salımlarda %6.6-18.3 ve %13.3-21.6’ya düşmüştür. Rhyzopertha dominica başlangıç ölüm oranları 7. ve 14. gün için %46.6-50.0 ve %93.3-95.0’dır ve 28 gün sonraki salımlar sonucunda %10.0-18.3 ve %16.6-28.3 olmuştur. Oryzaephilus surinamensis başlangıç ölümleri ise 7. ve 14. gün için %41.6-46.6 ve %70.0-85.0’den 28 gün sonraki salımda %8.3-15.0 ve %13.3-20.0’a düşmüştür. Buğdayda sporların bekleme süresi uzadıkça, tüm fungus izolatları ve böcek türleri için her inkübasyon süreci sonrasında ölüm oranları önemli derecede düşmüştür. İnkübasyon süreçlerinin tümünde tüm izolatlar için benzer ölüm oranları belirlenmiş ve etkinlik kayıplarının benzer olduğu gözlenmiştir. Tüm sonuçlar B. bassiana izolatlarında zamana bağlı aktivite kaybından sorumlu faktörlerin belirlenmesi ve bu veriler doğrultusunda önlem alınması gerektiğini göstermektedir.

Keywords

Biyolojik mücadele Mikrobiyal mücadele Entomopatojen fungus Beauveria bassiana Depolanmış ürün zararlıları

Supporting Institution

Kahramanmaraş Sütçü İmam Üniversitesi, Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

Project No: 2013/1-21D

References

  • Abbott, W.S 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18: 265-267.
  • Anonymous, 2021. TUİK Bitkisel Üretim İstatistikleri. URL (erişim tarihi: 28.12.2021) (https://data.tuik.gov.tr/ Kategori/GetKategori?p =tarim-111&dil=1)
  • Aregger E 1992. Conidia production of the fungus Beauveria brongniartii on barley and quality evaluation during storage at 2°C. J. of Invert. Patholog. 1992;5, 2–10.
  • Batta YA 2003. Production and testing of novel formulation of the entomopathogenic fungus Metarhizium anisopliae (Metch.) Sorokin (Deuteromycetes: Hyphomycetes). Crop Protectin 22, 415–422.
  • Batta YA 2004. Control of rice weevil (Sitophilus oryzae L., Coleoptera: Curculionidae) with various formulations of Metarhizium anisoplie. Crop Protection 23(2):103-108. http://dx.doi.org/10.1016/ j.cropro.2003.07.001.
  • Batta YA 2016. Recent advances in formulation and application of entomopathogenic fungi for biocontrol of stored grain insects. Biocont. Sci. Technol. 26:1171–1183.
  • Batta YA, Kavallieratos NG 2018. The use of entomopathogenic fungi for the control of stored-grain insects. Int. J. Pest Manag. 64:77–87.
  • Blanford S, Jenkins NE, Christian R, Chan BH, Nardini L, Osae M, Koekemoer L, Coetzee M, Read AF, vanden MB 2012. Storage and Persistence of a Candidate Fungal Biopesticide for Use Against Adult Malaria Vectors. Malar. J. 11, 354.
  • Er MK, Tunaz H, Işıkber AA 2016. Improving the virulence of a native Beauveria bassiana isolate against Rhyzopertha dominica adults. 7th International Scientific Agriculture Symposium (Agrosym 2016). 6-9 October 2016, Jahorina, Bosnia and Herzegovina. pp: 1464-1469 DOI:10.7251/AGRENG1607221.
  • Faria MR, Hajek AE, Wraight SP 2009. Imbibitional Damage in Conidia of the Entomopathogenic Fungi Beauveria bassiana, Metarhizium acridum, and Metarhizium anisopliae. Biol. Control 51, 346–354.
  • Glare T, Caradus J, Gelernter W, Jackson T, Keyhani N, Köhl J, Marrone P, Morin L, Stewart A 2012. Have Biopesticides Come of Age? Trends Biotechnol. 30, 250–258.
  • Heviefo GA., Nyamador SW, Datinon BD, Glitho IA, Tamö M 2020. Comparative efficacy of endophytic versus foliar application of the entomopathogenic fungus Beauveria bassiana against the crucifer diamondback moth larvae for sustainable cabbage protection. Int. J. Biol. Chem. Sci., 14(4): 1448-1458. https://doi.org/ 10.4314/ijbcs.v14i4.22.
  • Jackson MA 1997. Optimizing nutritional conditions for the liquid culture production of effective fungal biological control agents. J Ind Microbiol Biotechnol 19:180–187.
  • Kaur S, Thakur A, Rajput M 2014. A laboratory assessment of the potential of Beauveria bassiana (Balsamo) Vuillemin as a biocontrol agent of Corcyra cephalonica Stainton (Lepidoptera: Pyrallidae). J Stored Products Res 59:185–189.
  • Kim JS, Kassa A, Skinner M, Hata T, Parker BL, 2011. Production of thermotolerant entomopathogenic fungal conidia on millet grain. Journal of Industrial Microbiology and Biotechnology, 38(6): 697–704.
  • Kim JC, Lee MR, Kim S, Lee SJ, Park SE, Baek S, Gasmi L, Shin TY, Kim JS 2019. Long-term Storage Stability of Beauveria bassiana ERL836 Granules As Fungal Biopesticide, Journal of Asia-Pacific Entomology 22 (2019) 537–542.
  • Korkmaz HY 2017. Depolanmış tahıl zararlılarındaki yerel fungal etmenlerin tespiti ve bazi depolanmiş tahil zararlisi coleoptera türlerine karşi kullanim olanaklari. Doktora Tezi. Kahramanmaraş Sütçü İmam Üniversitesi Fen Bilimleri Enstitüsü. Kahramanmaraş. 169s.
  • Moore D, Lord JC, Smith SM 2000. Pathogens. In: Subramanyam BH, Hagstrum DW (eds) Alternatives to pesticides in stored product IPM. Kluwer Academic Publishers, Dordrecht, pp 193–227.
  • Padmini PCP, Padmaja V 2010. Impact of different relative humidities on in vitro growth and sporulation of entomopathogenic fungal isolates of Beauveria species. Int J Pharm Biol Sci Arch 1:355–359.
  • Rangel DE, Braga GU, Fernandes ÉK, Keyser CA, Hallsworth JE, Roberts DW 2015. Stress Tolerance and Virulence of Insect-Pathogenic Fungi are Determined by Environmental Conditions During Conidial Formation. Curr. Genet. 61: 383–404.
  • Santos MP, Dias LP, Ferreira PC, Pasin LA, Rangel DE 2011. Cold activity and tolerance of the entomopathogenic fungus Tolypocladium spp. to UV-B irradiation and heat. J. Invertebr. Pathol. 108, 209–213.
  • Sewify GH, El Shabrawy HA, Eweis ME, Naroz MH 2014. Efficacy of Entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae for Controlling Certain Stored Product İnsects. Egyptian Journal of Biological Pest Control, 24 (1): 191-196.
  • Shin TY, Bae SM, Kim DJ, Yun HG, Woo SD 2017. Evaluation of virulence, tolerance to environmental factors and antimicrobial activities of entomopathogenic fungi against two-spotted spider mite, Tetranychus urticae. Mycoscience 58: 204–212.
  • Soetopo D 2004. Efficacy of selected Beauveria bassiana (Bals.) Vuill. İsolates in Combination with a Resistant Cotton Variety (PSB-Ct 9) Against the Cotton Bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) PhD diss., University of the Philippines Los Banos.
  • St. Leger RJ, Wang C 2010. Genetic engineering of fungal biocontrol agents to achieve greater efficacy against insect pests. Appl Microbiol Biotechnol 85:901–907.
  • Wakil W, Schmitt T, Kavallieratos NG 2020. Persistence and Efficacy of Enhanced Diatomaceous Earth, İmidacloprid, And Beauveria bassiana Against Three Coleopteran and One Psocid Stored-Grain İnsects Environmental Science and Pollution Research Https://Doi.Org/10.1007/ S11356-020-12304-8.

Persistence of Beauveria bassiana Efficacy on Wheat Grains to Protect Stored-Grains from Post-Treatment Pest Infestations

Year 2022, Volume: 25 Issue: Ek Sayı 1, 143 - 148, 30.12.2022

Huriye Yasemin Korkmaz , Mehmet Kubilay Er

https://doi.org/10.18016/ksutarimdoga.vi.1018408

Abstract

This study was carried out to determine the post-treatment efficacy of three local Beauveria bassiana isolates by applying them against adults of three common stored-product pests, Sitophilus oryzae L. (Coleoptera: Curculionidae) Rhyzopertha dominica F. (Col.: Bostrichidae) and Oryzaephilus surinamensis L. (Col.: Silvanidae).
For the biological tests, wheat grains were mixed with fungal spores at 1000 ppm (w/w) concentration then 20 adults were released 1, 15 and 28 days after the treatments. For the tests carried out with S. oryzae; the mortality rate was initially 33.3-41.6% and 68.3-76.6%; and declined to 6.6-18.3% and 13.3-21.6% on the 7th and 14th day, respectively, when released 28 days later. The initial mortality of R. dominica was 46.6-50.0 % and 93.3-95%; and became 10-18.3% and 16.6-28.3% when released 28 days later. Oryzaephilus surinamensis mortality on the 7th and 14th day was initially 41.6-46.6% and 70.0-85.0%, and decreased to 8.3-15% and 13.3-20.0%, respectively, when released 28 days later. Mortality rates at the end of each incubation period, for all testing isolates and insect species, were significantly reduced when waiting time of spores on wheat was prolonged. For all incubation periods, similar mortality rates were obtained from all isolates and similar efficiacy loss was observed. All the results together indicate that the responsible factors for time-dependent loss of activity in B. bassiana isolates should be determined, and in line with these data, precautions need to be taken.

Keywords

Biological control Microbial control Entomopathogenic fungi Beauveria bassiana Stored-product pests

Project Number

Project No: 2013/1-21D

References

  • Abbott, W.S 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18: 265-267.
  • Anonymous, 2021. TUİK Bitkisel Üretim İstatistikleri. URL (erişim tarihi: 28.12.2021) (https://data.tuik.gov.tr/ Kategori/GetKategori?p =tarim-111&dil=1)
  • Aregger E 1992. Conidia production of the fungus Beauveria brongniartii on barley and quality evaluation during storage at 2°C. J. of Invert. Patholog. 1992;5, 2–10.
  • Batta YA 2003. Production and testing of novel formulation of the entomopathogenic fungus Metarhizium anisopliae (Metch.) Sorokin (Deuteromycetes: Hyphomycetes). Crop Protectin 22, 415–422.
  • Batta YA 2004. Control of rice weevil (Sitophilus oryzae L., Coleoptera: Curculionidae) with various formulations of Metarhizium anisoplie. Crop Protection 23(2):103-108. http://dx.doi.org/10.1016/ j.cropro.2003.07.001.
  • Batta YA 2016. Recent advances in formulation and application of entomopathogenic fungi for biocontrol of stored grain insects. Biocont. Sci. Technol. 26:1171–1183.
  • Batta YA, Kavallieratos NG 2018. The use of entomopathogenic fungi for the control of stored-grain insects. Int. J. Pest Manag. 64:77–87.
  • Blanford S, Jenkins NE, Christian R, Chan BH, Nardini L, Osae M, Koekemoer L, Coetzee M, Read AF, vanden MB 2012. Storage and Persistence of a Candidate Fungal Biopesticide for Use Against Adult Malaria Vectors. Malar. J. 11, 354.
  • Er MK, Tunaz H, Işıkber AA 2016. Improving the virulence of a native Beauveria bassiana isolate against Rhyzopertha dominica adults. 7th International Scientific Agriculture Symposium (Agrosym 2016). 6-9 October 2016, Jahorina, Bosnia and Herzegovina. pp: 1464-1469 DOI:10.7251/AGRENG1607221.
  • Faria MR, Hajek AE, Wraight SP 2009. Imbibitional Damage in Conidia of the Entomopathogenic Fungi Beauveria bassiana, Metarhizium acridum, and Metarhizium anisopliae. Biol. Control 51, 346–354.
  • Glare T, Caradus J, Gelernter W, Jackson T, Keyhani N, Köhl J, Marrone P, Morin L, Stewart A 2012. Have Biopesticides Come of Age? Trends Biotechnol. 30, 250–258.
  • Heviefo GA., Nyamador SW, Datinon BD, Glitho IA, Tamö M 2020. Comparative efficacy of endophytic versus foliar application of the entomopathogenic fungus Beauveria bassiana against the crucifer diamondback moth larvae for sustainable cabbage protection. Int. J. Biol. Chem. Sci., 14(4): 1448-1458. https://doi.org/ 10.4314/ijbcs.v14i4.22.
  • Jackson MA 1997. Optimizing nutritional conditions for the liquid culture production of effective fungal biological control agents. J Ind Microbiol Biotechnol 19:180–187.
  • Kaur S, Thakur A, Rajput M 2014. A laboratory assessment of the potential of Beauveria bassiana (Balsamo) Vuillemin as a biocontrol agent of Corcyra cephalonica Stainton (Lepidoptera: Pyrallidae). J Stored Products Res 59:185–189.
  • Kim JS, Kassa A, Skinner M, Hata T, Parker BL, 2011. Production of thermotolerant entomopathogenic fungal conidia on millet grain. Journal of Industrial Microbiology and Biotechnology, 38(6): 697–704.
  • Kim JC, Lee MR, Kim S, Lee SJ, Park SE, Baek S, Gasmi L, Shin TY, Kim JS 2019. Long-term Storage Stability of Beauveria bassiana ERL836 Granules As Fungal Biopesticide, Journal of Asia-Pacific Entomology 22 (2019) 537–542.
  • Korkmaz HY 2017. Depolanmış tahıl zararlılarındaki yerel fungal etmenlerin tespiti ve bazi depolanmiş tahil zararlisi coleoptera türlerine karşi kullanim olanaklari. Doktora Tezi. Kahramanmaraş Sütçü İmam Üniversitesi Fen Bilimleri Enstitüsü. Kahramanmaraş. 169s.
  • Moore D, Lord JC, Smith SM 2000. Pathogens. In: Subramanyam BH, Hagstrum DW (eds) Alternatives to pesticides in stored product IPM. Kluwer Academic Publishers, Dordrecht, pp 193–227.
  • Padmini PCP, Padmaja V 2010. Impact of different relative humidities on in vitro growth and sporulation of entomopathogenic fungal isolates of Beauveria species. Int J Pharm Biol Sci Arch 1:355–359.
  • Rangel DE, Braga GU, Fernandes ÉK, Keyser CA, Hallsworth JE, Roberts DW 2015. Stress Tolerance and Virulence of Insect-Pathogenic Fungi are Determined by Environmental Conditions During Conidial Formation. Curr. Genet. 61: 383–404.
  • Santos MP, Dias LP, Ferreira PC, Pasin LA, Rangel DE 2011. Cold activity and tolerance of the entomopathogenic fungus Tolypocladium spp. to UV-B irradiation and heat. J. Invertebr. Pathol. 108, 209–213.
  • Sewify GH, El Shabrawy HA, Eweis ME, Naroz MH 2014. Efficacy of Entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae for Controlling Certain Stored Product İnsects. Egyptian Journal of Biological Pest Control, 24 (1): 191-196.
  • Shin TY, Bae SM, Kim DJ, Yun HG, Woo SD 2017. Evaluation of virulence, tolerance to environmental factors and antimicrobial activities of entomopathogenic fungi against two-spotted spider mite, Tetranychus urticae. Mycoscience 58: 204–212.
  • Soetopo D 2004. Efficacy of selected Beauveria bassiana (Bals.) Vuill. İsolates in Combination with a Resistant Cotton Variety (PSB-Ct 9) Against the Cotton Bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) PhD diss., University of the Philippines Los Banos.
  • St. Leger RJ, Wang C 2010. Genetic engineering of fungal biocontrol agents to achieve greater efficacy against insect pests. Appl Microbiol Biotechnol 85:901–907.
  • Wakil W, Schmitt T, Kavallieratos NG 2020. Persistence and Efficacy of Enhanced Diatomaceous Earth, İmidacloprid, And Beauveria bassiana Against Three Coleopteran and One Psocid Stored-Grain İnsects Environmental Science and Pollution Research Https://Doi.Org/10.1007/ S11356-020-12304-8.
There are 26 citations in total.

Details

Primary Language English
Subjects Agricultural, Veterinary and Food Sciences
Journal Section RESEARCH ARTICLE
Authors

Huriye Yasemin Korkmaz 0000-0002-7586-1812

Mehmet Kubilay Er 0000-0003-1568-8656

Project Number Project No: 2013/1-21D
Publication Date December 30, 2022
Submission Date November 3, 2021
Acceptance Date December 29, 2021
Published in Issue Year 2022Volume: 25 Issue: Ek Sayı 1

Cite

APA Korkmaz, H. Y., & Er, M. K. (2022). Persistence of Beauveria bassiana Efficacy on Wheat Grains to Protect Stored-Grains from Post-Treatment Pest Infestations. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(Ek Sayı 1), 143-148. https://doi.org/10.18016/ksutarimdoga.vi.1018408
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