Molecular Characterization and Assessment of Population Structure of Hulled Wheats

Fatih Demirel; Kahraman Gürcan; Taner Akar

doi:10.18016/ksutarimdoga.vi.968009

TR EN

Kavuzlu Buğdayların Moleküler Karakterizasyonu ve Popülasyon Yapısının Değerlendirilmesi

Öz

Genotipler arasındaki genetik çeşitliliğin ve popülasyonlar arasındaki farklılaşmanın analizi, koruma stratejilerinin belirlenmesi ve en iyi bitki ıslah yaklaşımlarından biri için çok önemlidir. 23 kavuzlu buğdayda genetik varyasyonu ve popülasyon yapısını belirlemek için ISSR markörleri kullanılmıştır. Kontrol makarnalık buğday ve ekmeklik buğday tescilli çeşitleriyle birlikte, 14 ISSR markörü ile 32 buğday genotipi analiz edildi. Lokus başına alel sayısı 3 ile 13 arasında ve polimorfizm bilgi içeriği (PIC) değeri UBC-852 için 0.27 ile UBC-824 için 0.37 arasında ve ortalama 0.33 olarak belirlenmiştir. ISSR primerleri için yüksek düzeyde polimorfizm oranı (%100) gözlenmiştir. Ortalama polimorfik alel sayısı (N), beklenen heterozigotluk (He), PIC, etkili allel sayısı (Ne), Shanon bilgi indeksi (I) ve genetik varyasyon (FST) sırasıyla 10.21, 0.42, 0.33, 1.78, 0.61 ve 0,63 olarak belirlenmiştir. Dice genetik benzerliğine dayanan UPGMA analizi, kavuzlu buğday genotipleri arasındaki yüksek genetik çeşitliliği göstermiş olup 0.981 ile 0.112 arasında değişmiştir. Sonuçlar, ISSR markörlerinin kavuzlu buğday genotiplerinin popülasyon yapısı ve genetik çeşitliliğinin değerlendirilmesi için güvenilir ve tekrarlanabilir parmak izi profilleri sağladığını göstermiştir. Mevcut çalışmadan elde edilen bu moleküler varyasyonlar, ıslah çalışmaları için ebeveyn seçiminde kullanılabilir.

Anahtar Kelimeler

Supporting Institution

Erciyes University

Project Number

FYL-2013-4233

References

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Fatih Demirel ^*
0000-0002-6846-8422
Türkiye

Kahraman Gürcan
0000-0002-2120-2563
Türkiye

Taner Akar
0000-0002-3488-3943
Türkiye

Publication Date

December 30, 2022

Submission Date

July 9, 2021

Acceptance Date

February 17, 2022

Published in Issue

Year 1970 Volume: 25 Number: Ek Sayı 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.968009

IZ

https://izlik.org/JA89CU83BF

Cite

RIS / Bibtex

APA

Demirel, F., Gürcan, K., & Akar, T. (2022). Molecular Characterization and Assessment of Population Structure of Hulled Wheats. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(Ek Sayı 1), 192-199. https://doi.org/10.18016/ksutarimdoga.vi.968009

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Molecular Characterization and Assessment of Population Structure of Hulled Wheats

Kavuzlu Buğdayların Moleküler Karakterizasyonu ve Popülasyon Yapısının Değerlendirilmesi

Öz

Anahtar Kelimeler