Abstract
Analysis of genetic diversity among genotypes and differentiation among populations are crucial for determination of conservation strategies and one of the best plant breeding approaches. In this study, ISSR markers were used to determine genetic variation and population structure in 23 hulled wheats. Together with control durum wheat and bread wheat registered cultivars, 32 wheat genotypes were analyzed with 14 ISSR markers located throughout the wheat genome. Number of alleles per locus ranged from 3 to 13 and the polymorphism information content (PIC) value ranged from 0.27 for the UBC-852 to 0.37 for the UBC-824 with an average of 0.33. High levels of polymorphism ratio (100%) were observed for ISSR primers. Mean number of polymorphic alleles (N), expected heterozygosity (He), PIC, number of effective allele (Ne), Shanon’s information index (I) and genetic variation (FST) were determined as 10.21, 0.42, 0.33, 1.78, 0.61 and 0.63, respectively. UPGMA analysis based on dice genetic similarity ranged between 0.981 and 0.112 showing the high genetic diversity among hulled wheat genotypes. Results showed that the ISSR markers provided reliable and reproducible fingerprinting profiles for assessment of population structure and genetic diversity of hulled wheat genotypes. These molecular variations obtained from present study can be used in parent choosing for breeding studies.
Keywords
Supporting Institution
Erciyes University
Project Number
FYL-2013-4233
References
- Abou-Deif MH, Rashed MA, Sallam MAA, Mostafa EAH, Ramadan WA 2013. Characterization of Twenty Wheat Varieties by ISSR Markers. Middle East Journal of Scientific Research 15(2): 168-175.
- Carvalho A, Lima-Brito J, Maçãs B, Guedes-Pinto H 2009. Genetic Diversity and Variation Among Botanical Varieties of Old Portuguese Wheat Cultivars Revealed by ISSR Assays. Biochemical Genetics 47(3-4): 276-294.
- Du JK, Yao YY, Ni ZF, Peng HR, Sun QX 2002. Genetic Diversity Revealed by ISSR Molecular Marker in Common Wheat, Spelt, Compactum and Progeny of Recurrent Selection. Yi Chuan Xue Bao= Acta Genetica Sinica 29(5): 445-452.
- El Maati FB, Jlibene M, Moumni M 2004. Study of The Polymorphism of Common Wheat Using ISSR Markers. Journal of Food, Agriculture and Environment 2(3-4): 121-125.
- Evanno G, Regnaut S, Goudet J 2005. Detecting The Number of Clusters of Individuals Using The Software Structure: A Simulation Study. Molecular Ecology 14(8): 2611-2620.
- Fahmy EM, Abd El-Gawad NM, El-Geddawy IH, Saleh OM, El-Azab NM 2016. Development of RAPD and ISSR Markers for Drought Tolerance in Sugarcane (Saccharum officinarum L.). Egyptian Journal of Genetics and Cytology 37(1): 1-15.
- Gulbitti O, Sümer S, Özcan S 2007. Determination of Phylogenetic Relationships Between Some Wild Wheat Species Using Amplified Fragment Length Polymorphism (AFLP) Markers. Botanical Journal of the Linnean Society 153(1): 67-72.
- Gulsen O, Shearman RC, Vogel KP, Lee DJ, Baenziger PS, Heng-Moss TM, Budak H 2005. Nuclear Genome Diversity and relationships Among Naturally Occurring Buffalograss Genotypes Determined by Sequence-Related Amplified Polymorphism Markers. Hort Science 40(3): 537-541.
- Gupta M, Chyi YS, Romero-Severson J, Owen JL 1994. Amplification of DNA Markers from Evolutionarily Diverse Genomes Using Single Primers of Simple-Sequence Repeats. Theoretical and Applied Genetics 89(7-8): 998-1006.
- Gurcan K, Demirel F, Tekin M, Demirel S, Akar T 2017. Molecular and Agro-Morphological Characterization of Ancient Wheat Landraces of Turkey. BMC Plant Biology 17 (1):171.
- Harlan JR 1998. The Living Fields: Our Agricultural Heritage. Cambridge University Press, UK.
- Heun M, Schäfer-Pregl R, Klawan D, Castagna R, Accerbi M, Borghi B, Salamini F 1997. Site of Einkorn Wheat Domestication Identified by DNA Fingerprinting. Science 278(5341): 1312-1314.
- Karaca M, Izbirak A 2008. Comparative Analysis of Genetic Diversity in Turkish Durum Wheat Cultivars Using RAPD and ISSR Markers. Journal of Food, Agriculture and Environment 6: 219-225.
- Karagoz A 1996. Agronomic Practices and Socioeconomic Aspects of Emmer and Einkorn Cultivation in Turkey. Hulled Wheat. Promoting the Conservation and Use of Underutilized and Neglected Crops 4: 172-177.
- Karagoz A, Zencirci N 2005. Variation in Wheat (Triticum spp.) Landraces from Different Altitudes of Three Regions of Turkey. Genetic Resources and Crop Evolution 52(6): 775-785.
- Khush GS 2002. Molecular Genetics—Plant Breeder’s Perspective. In Molecular Techniques in Crop Improvement. Springer, Dordrecht.
- Morgante M, Hanafey M, Powell W 2002. Microsatellites are Preferentially Associated with Nonrepetitive DNA in Plant Genomes. Nature Genetics 30(2): 194-200.
- Motawei MI, Al-Doss AA, Moustafa KA 2007. Genetic Diversity among Selected Wheat Lines Differing in Heat Tolerance Using Molecular Markers. Journal of Food Agriculture and Environment 5(1): 180.
- Najaphy A, Parchin RA, Farshadfar E 2012. Comparison of Phenotypic and Molecular Characterizations of Some Important Wheat Cultivars and Advanced Breeding Lines. Australian Journal of Crop Science 6(2): 326-332.
- Najaphy A, Parchin RA, Farshadfar E 2011. Evaluation of Genetic Diversity in Wheat Cultivars and Breeding Lines Using Inter Simple Sequence Repeat Markers. Biotechnology and Biotechnological Equipment 25(4): 2634-2638.
- Nei M 1973. Analysis of Gene Diversity in Subdivided Populations. Proceedings of the National Academy of Sciences 70(12): 3321-3323.
- Prasad M, Varshney RK, Roy JK, Balyan HS, Gupta PK 2000. The Use of Microsatellites for Detecting DNA Polymorphism, Genotype Identification and Genetic Diversity in Wheat. Theoretical and Applied Genetics 100(3-4): 584-592.
- Pritchard JK, Stephens M, Donnelly P 2000. Inference of Population Structure Using Multilocus Genotype Data. Genetics 155(2): 945-959.
- Rohlf FJ 2000. NTSYS-pc: Numerical Taxonomy (Vitis vinifera L.) Varieties Using Morphological Data and Multivariate Analysis System. Exeter Software and AFLP markers. Electronic Journal of Biotechnology 6: 37-45.
- Sofalian O, Chaparzadeh N, Javanmard A, Hejazi MS 2008. Study The Genetic Diversity of Wheat Landraces From Northwest of Iran Based on ISSR Molecular Markers. Internationa Journal Agriculture Biology 10(3): 466-468.
- Yang W, de Oliveira AC, Godwin I, Schertz K, Bennetzen JL 1996. Comparison of DNA Marker Technologies in Characterizing Plant Genome Diversity: Variability in Chinese Sorghums. Crop Science 36(6): 1669-1676.
Abstract
Genotipler arasındaki genetik çeşitliliğin ve popülasyonlar arasındaki farklılaşmanın analizi, koruma stratejilerinin belirlenmesi ve en iyi bitki ıslah yaklaşımlarından biri için çok önemlidir. 23 kavuzlu buğdayda genetik varyasyonu ve popülasyon yapısını belirlemek için ISSR markörleri kullanılmıştır. Kontrol makarnalık buğday ve ekmeklik buğday tescilli çeşitleriyle birlikte, 14 ISSR markörü ile 32 buğday genotipi analiz edildi. Lokus başına alel sayısı 3 ile 13 arasında ve polimorfizm bilgi içeriği (PIC) değeri UBC-852 için 0.27 ile UBC-824 için 0.37 arasında ve ortalama 0.33 olarak belirlenmiştir. ISSR primerleri için yüksek düzeyde polimorfizm oranı (%100) gözlenmiştir. Ortalama polimorfik alel sayısı (N), beklenen heterozigotluk (He), PIC, etkili allel sayısı (Ne), Shanon bilgi indeksi (I) ve genetik varyasyon (FST) sırasıyla 10.21, 0.42, 0.33, 1.78, 0.61 ve 0,63 olarak belirlenmiştir. Dice genetik benzerliğine dayanan UPGMA analizi, kavuzlu buğday genotipleri arasındaki yüksek genetik çeşitliliği göstermiş olup 0.981 ile 0.112 arasında değişmiştir. Sonuçlar, ISSR markörlerinin kavuzlu buğday genotiplerinin popülasyon yapısı ve genetik çeşitliliğinin değerlendirilmesi için güvenilir ve tekrarlanabilir parmak izi profilleri sağladığını göstermiştir. Mevcut çalışmadan elde edilen bu moleküler varyasyonlar, ıslah çalışmaları için ebeveyn seçiminde kullanılabilir.
Keywords
Project Number
FYL-2013-4233
References
- Abou-Deif MH, Rashed MA, Sallam MAA, Mostafa EAH, Ramadan WA 2013. Characterization of Twenty Wheat Varieties by ISSR Markers. Middle East Journal of Scientific Research 15(2): 168-175.
- Carvalho A, Lima-Brito J, Maçãs B, Guedes-Pinto H 2009. Genetic Diversity and Variation Among Botanical Varieties of Old Portuguese Wheat Cultivars Revealed by ISSR Assays. Biochemical Genetics 47(3-4): 276-294.
- Du JK, Yao YY, Ni ZF, Peng HR, Sun QX 2002. Genetic Diversity Revealed by ISSR Molecular Marker in Common Wheat, Spelt, Compactum and Progeny of Recurrent Selection. Yi Chuan Xue Bao= Acta Genetica Sinica 29(5): 445-452.
- El Maati FB, Jlibene M, Moumni M 2004. Study of The Polymorphism of Common Wheat Using ISSR Markers. Journal of Food, Agriculture and Environment 2(3-4): 121-125.
- Evanno G, Regnaut S, Goudet J 2005. Detecting The Number of Clusters of Individuals Using The Software Structure: A Simulation Study. Molecular Ecology 14(8): 2611-2620.
- Fahmy EM, Abd El-Gawad NM, El-Geddawy IH, Saleh OM, El-Azab NM 2016. Development of RAPD and ISSR Markers for Drought Tolerance in Sugarcane (Saccharum officinarum L.). Egyptian Journal of Genetics and Cytology 37(1): 1-15.
- Gulbitti O, Sümer S, Özcan S 2007. Determination of Phylogenetic Relationships Between Some Wild Wheat Species Using Amplified Fragment Length Polymorphism (AFLP) Markers. Botanical Journal of the Linnean Society 153(1): 67-72.
- Gulsen O, Shearman RC, Vogel KP, Lee DJ, Baenziger PS, Heng-Moss TM, Budak H 2005. Nuclear Genome Diversity and relationships Among Naturally Occurring Buffalograss Genotypes Determined by Sequence-Related Amplified Polymorphism Markers. Hort Science 40(3): 537-541.
- Gupta M, Chyi YS, Romero-Severson J, Owen JL 1994. Amplification of DNA Markers from Evolutionarily Diverse Genomes Using Single Primers of Simple-Sequence Repeats. Theoretical and Applied Genetics 89(7-8): 998-1006.
- Gurcan K, Demirel F, Tekin M, Demirel S, Akar T 2017. Molecular and Agro-Morphological Characterization of Ancient Wheat Landraces of Turkey. BMC Plant Biology 17 (1):171.
- Harlan JR 1998. The Living Fields: Our Agricultural Heritage. Cambridge University Press, UK.
- Heun M, Schäfer-Pregl R, Klawan D, Castagna R, Accerbi M, Borghi B, Salamini F 1997. Site of Einkorn Wheat Domestication Identified by DNA Fingerprinting. Science 278(5341): 1312-1314.
- Karaca M, Izbirak A 2008. Comparative Analysis of Genetic Diversity in Turkish Durum Wheat Cultivars Using RAPD and ISSR Markers. Journal of Food, Agriculture and Environment 6: 219-225.
- Karagoz A 1996. Agronomic Practices and Socioeconomic Aspects of Emmer and Einkorn Cultivation in Turkey. Hulled Wheat. Promoting the Conservation and Use of Underutilized and Neglected Crops 4: 172-177.
- Karagoz A, Zencirci N 2005. Variation in Wheat (Triticum spp.) Landraces from Different Altitudes of Three Regions of Turkey. Genetic Resources and Crop Evolution 52(6): 775-785.
- Khush GS 2002. Molecular Genetics—Plant Breeder’s Perspective. In Molecular Techniques in Crop Improvement. Springer, Dordrecht.
- Morgante M, Hanafey M, Powell W 2002. Microsatellites are Preferentially Associated with Nonrepetitive DNA in Plant Genomes. Nature Genetics 30(2): 194-200.
- Motawei MI, Al-Doss AA, Moustafa KA 2007. Genetic Diversity among Selected Wheat Lines Differing in Heat Tolerance Using Molecular Markers. Journal of Food Agriculture and Environment 5(1): 180.
- Najaphy A, Parchin RA, Farshadfar E 2012. Comparison of Phenotypic and Molecular Characterizations of Some Important Wheat Cultivars and Advanced Breeding Lines. Australian Journal of Crop Science 6(2): 326-332.
- Najaphy A, Parchin RA, Farshadfar E 2011. Evaluation of Genetic Diversity in Wheat Cultivars and Breeding Lines Using Inter Simple Sequence Repeat Markers. Biotechnology and Biotechnological Equipment 25(4): 2634-2638.
- Nei M 1973. Analysis of Gene Diversity in Subdivided Populations. Proceedings of the National Academy of Sciences 70(12): 3321-3323.
- Prasad M, Varshney RK, Roy JK, Balyan HS, Gupta PK 2000. The Use of Microsatellites for Detecting DNA Polymorphism, Genotype Identification and Genetic Diversity in Wheat. Theoretical and Applied Genetics 100(3-4): 584-592.
- Pritchard JK, Stephens M, Donnelly P 2000. Inference of Population Structure Using Multilocus Genotype Data. Genetics 155(2): 945-959.
- Rohlf FJ 2000. NTSYS-pc: Numerical Taxonomy (Vitis vinifera L.) Varieties Using Morphological Data and Multivariate Analysis System. Exeter Software and AFLP markers. Electronic Journal of Biotechnology 6: 37-45.
- Sofalian O, Chaparzadeh N, Javanmard A, Hejazi MS 2008. Study The Genetic Diversity of Wheat Landraces From Northwest of Iran Based on ISSR Molecular Markers. Internationa Journal Agriculture Biology 10(3): 466-468.
- Yang W, de Oliveira AC, Godwin I, Schertz K, Bennetzen JL 1996. Comparison of DNA Marker Technologies in Characterizing Plant Genome Diversity: Variability in Chinese Sorghums. Crop Science 36(6): 1669-1676.
Details
| Primary Language | English |
|---|---|
| Subjects | Agricultural, Veterinary and Food Sciences |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Project Number | FYL-2013-4233 |
| Publication Date | December 30, 2022 |
| Submission Date | July 9, 2021 |
| Acceptance Date | February 17, 2022 |
| Published in Issue | Year 2022Volume: 25 Issue: Ek Sayı 1 |
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