Molecular Characterization and Assessment of Population Structure of Hulled Wheats

Fatih Demirel; Kahraman Gürcan; Taner Akar

doi:10.18016/ksutarimdoga.vi.968009

TR EN

Kavuzlu Buğdayların Moleküler Karakterizasyonu ve Popülasyon Yapısının Değerlendirilmesi

Öz

Genotipler arasındaki genetik çeşitliliğin ve popülasyonlar arasındaki farklılaşmanın analizi, koruma stratejilerinin belirlenmesi ve en iyi bitki ıslah yaklaşımlarından biri için çok önemlidir. 23 kavuzlu buğdayda genetik varyasyonu ve popülasyon yapısını belirlemek için ISSR markörleri kullanılmıştır. Kontrol makarnalık buğday ve ekmeklik buğday tescilli çeşitleriyle birlikte, 14 ISSR markörü ile 32 buğday genotipi analiz edildi. Lokus başına alel sayısı 3 ile 13 arasında ve polimorfizm bilgi içeriği (PIC) değeri UBC-852 için 0.27 ile UBC-824 için 0.37 arasında ve ortalama 0.33 olarak belirlenmiştir. ISSR primerleri için yüksek düzeyde polimorfizm oranı (%100) gözlenmiştir. Ortalama polimorfik alel sayısı (N), beklenen heterozigotluk (He), PIC, etkili allel sayısı (Ne), Shanon bilgi indeksi (I) ve genetik varyasyon (FST) sırasıyla 10.21, 0.42, 0.33, 1.78, 0.61 ve 0,63 olarak belirlenmiştir. Dice genetik benzerliğine dayanan UPGMA analizi, kavuzlu buğday genotipleri arasındaki yüksek genetik çeşitliliği göstermiş olup 0.981 ile 0.112 arasında değişmiştir. Sonuçlar, ISSR markörlerinin kavuzlu buğday genotiplerinin popülasyon yapısı ve genetik çeşitliliğinin değerlendirilmesi için güvenilir ve tekrarlanabilir parmak izi profilleri sağladığını göstermiştir. Mevcut çalışmadan elde edilen bu moleküler varyasyonlar, ıslah çalışmaları için ebeveyn seçiminde kullanılabilir.

Anahtar Kelimeler

Molecular Characterization and Assessment of Population Structure of Hulled Wheats

Abstract

Analysis of genetic diversity among genotypes and differentiation among populations are crucial for determination of conservation strategies and one of the best plant breeding approaches. In this study, ISSR markers were used to determine genetic variation and population structure in 23 hulled wheats. Together with control durum wheat and bread wheat registered cultivars, 32 wheat genotypes were analyzed with 14 ISSR markers located throughout the wheat genome. Number of alleles per locus ranged from 3 to 13 and the polymorphism information content (PIC) value ranged from 0.27 for the UBC-852 to 0.37 for the UBC-824 with an average of 0.33. High levels of polymorphism ratio (100%) were observed for ISSR primers. Mean number of polymorphic alleles (N), expected heterozygosity (He), PIC, number of effective allele (Ne), Shanon’s information index (I) and genetic variation (FST) were determined as 10.21, 0.42, 0.33, 1.78, 0.61 and 0.63, respectively. UPGMA analysis based on dice genetic similarity ranged between 0.981 and 0.112 showing the high genetic diversity among hulled wheat genotypes. Results showed that the ISSR markers provided reliable and reproducible fingerprinting profiles for assessment of population structure and genetic diversity of hulled wheat genotypes. These molecular variations obtained from present study can be used in parent choosing for breeding studies.

Keywords

Destekleyen Kurum

Erciyes University

Proje Numarası

FYL-2013-4233

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Ziraat, Veterinerlik ve Gıda Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Fatih Demirel ^*
0000-0002-6846-8422
Türkiye

Kahraman Gürcan
0000-0002-2120-2563
Türkiye

Taner Akar
0000-0002-3488-3943
Türkiye

Yayımlanma Tarihi

30 Aralık 2022

Gönderilme Tarihi

9 Temmuz 2021

Kabul Tarihi

17 Şubat 2022

Yayımlandığı Sayı

Yıl 2022 Cilt: 25 Sayı: Ek Sayı 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.968009

IZ

https://izlik.org/JA89CU83BF

Kaynak Göster

RIS / Bibtex

APA

Demirel, F., Gürcan, K., & Akar, T. (2022). Molecular Characterization and Assessment of Population Structure of Hulled Wheats. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(Ek Sayı 1), 192-199. https://doi.org/10.18016/ksutarimdoga.vi.968009