Research Article
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Detection and Molecular Characterization of Tobacco mild green mosaic virus Isolates Infecting Peppers in Turkey

Year 2022, Volume: 25 Issue: Ek Sayı 2, 490 - 496, 30.12.2022

Selin Ceren Balsak Seher Gül Avcu Nihal Buzkan

https://doi.org/10.18016/ksutarimdoga.vi.1048580

Abstract

In this study, Tobacco mild green mosaic virus (TMGMV) infection was investigated in pepper crops from two regions having high economical importance in Turkey. A total of 397 leaf and fruit samples showing mosaic, yellowing, mottling, and pitting symptoms were collected to test by double antibody sandwich ELISA (DAS-ELISA) using polyclonal antiserum. DAS-ELISA results indicated that 97 out of all tested samples (24.4%) were found to be infected with TMGMV. Samples resulting positive for TMGMV infection were used to amplify the virus coat protein (CP) gene sequences with specific primers by RT-PCR for further molecular characterization of the virus isolates. A comparison of the CP sequences of the virus isolates revealed that the identity of nucleotides ranged between 97.2 and 100%, and the amino acid similarity ranged between 96.8 and 100% among themselves. To the best of our knowledge, this is the first report of TMGMV infection in Capsicum annuum in Turkey.

Keywords

Tobamovirus TMGMV Capsicum annuum DAS-ELISA Coat protein

Supporting Institution

This research was funded by the the Research Fund of Kahramanmaraş Sütçü Imam University

Project Number

2021/1-15YLS

References

  • Alonso E, Garcı´a-Luque I, Avila-Rinco´n MJ, Wicke B, Serra MT, Dı´az-Ruı´z JR 1989. A tobamovirus causing heavy losses in protected pepper crops in Spain. Journal of Phytopathology, 125(1):67–76.
  • Broadbent L 1965. The epidemiology of tomato mosaic. XI. Seed transmission of TMV. The Annals of Applied Biology, 56(2):177-205.
  • Buzkan N, Arpacı BB 2017. New emerging viruses in pepper crops in Turkey. 15th Congress of the Mediterranean Phytopathological Union, June 20-23, 2017, Cordoba, Spain.
  • Çağlar B, Fidan KH, Elbeaino T 2013. Detection and Molecular Characterization of Pepper Mild Mottle Virus from Turkey. Journal of Phytopathology, 161 (6): 434-438.
  • Chomczynski P, Sacchi N 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry, 162(1):156-9. doi: 10.1006/abio.1987. 9999. PMID: 2440339.
  • Clark MF, Adams AN 1977. Characteristic of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology, 34(3): 475–483.
  • Faostat 2020. Food And Agriculture Organization. Statistical Databases Web Page (www.faostat.fao .org).
  • Fidan H, Sarikaya P, Yildiz K, Topkaya B, Erkis G, Calis O 2021. Robust molecular detection of the new Tomato brown rugose fruit virus in infected tomato and pepper plants from Turkey, Journal of Integrative Agriculture, 20(8): 2170-2179.
  • Green SK, Kim JS 1994. Source of resistance to viruses of pepper (Capsicum spp.): A catalog. Technical Bulletin, 20, AVRDC, 64.
  • Jarret RL, Gillaspie AG, Barkley NA, Pinnow DL 2008. The occurrence and control of pepper mild mottle virus (PMMoV) in the USDA/ARS. Capsicum germplasm collection. Seed technology, 26-36. https://naldc.nal.usda.gov/download/26970/PDF.
  • Karanfil A, Sarı M, Korkmaz S 2020. First report of tobacco mild green mosaic virus in Turkey. Journal of Plant Pathology, 102, 547.
  • Kenyon L, Kumar S, Tsai WS, Hughes JD 2014. Virus diseases of peppers (Capsicum spp.) and their control. Advances in Virus Research, 90:297‐354. doi:10.1016/B978-0-12-801246-8.00006-8.
  • Kumar S, Stecher G, Tamura K 2016. Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7):1870–1874.
  • Lanter JM, McGuire JM, Goode M 1982. Persistence of tomato mosaic virus in tomato debris and soil under field conditions. Plant Disease, 66(7): 552-555.
  • Letschert B, Adam G, Lesemann DE, Willingmann P, Heinze C 2002. Detection and differentiation of serologically crossreacting tobamoviruses of economical importance by RTPCR and RT-PCR-RFLP. Journal of Virological Methods, 106(1):1–10.
  • Moury B, Morel C, Johansen E, Guilbaud L, Souche S, Ayme V, Caranta C, Palloix A, Jacquemond M. 2004. Mutations in potato virus Y genome-linked protein determine virulence toward recessive resistances in Capsicum annuum and Lycopersicon hirsutum. Molecular Plant-Microbe Interaction, 17(3):322-9. doi: 10.1094/MPMI. 2004.17.3.322. PMID: 15000399.
  • Moury B, Verdin E 2012. Viruses of pepper crops in the Mediterranean basin: a remarkable stasis. Advances in Virus Research, 84: 127–162.
  • Ozaslan M, Bas B, Aytekin T, Sigirci Z 2006. Identification of Pepper Viruses by Das-elisa Assays in Gaziantep-Turkey. Plant Pathology Journal, 5(1):11-14.
  • Pares RD, Gunn LV, Keskula EN 1996. The role of infective plant debris, and its concentration in soil, in the ecology of tomato mosaic tobamovirus—A non-vectored plant virus. Journal of Phytopathology-Phytopathologische Zeitschrift, 144(3):147-150.
  • Pernezny KL, Roberts PD, Murphy JF, Goldberg NP 2003. Compendium of pepper diseases. St. Paul, MN: APS Press.
  • Rast ATB, Stijger CCMM 1987. Disinfection of pepper seed infected with different strains of capsicum mosaic virus by trisodium phosphate and dry heat treatment. Plant Pathology, 36(4): 583–588.
  • Salamon P, Kaszta M 2000. Investigation on the transmission of some Tobamoviruses by pollen and seed in pepper (Capsicum annuum L.). In: 8 The International Pollination Symposium, Mosonmagyarovar, Hungary, 10–14 July, 2000. International Journal of Horticultural Science, 6: 127 –131.
  • Şevik MA 2011. Occurrence of pepper mild mottle virus in greenhouse grown pepper (Capsicum annuum L.) in the Test Mediterranean region of Turkey. African Journal of Biotechnology, 10(25): 4976-4979.
  • Şimşek D, Pınar H, Mutlu N 2015. Moleküler Islah Yöntemleri Kullanılarak Tospovirus ve Tobamoviruslere Dayanıklı Çarli Biber (Capsicum annum L.) Hat ve Çeşitlerinin Geliştirilmesi. Selçuk Tarım Bilimleri Dergisi, 1(1):1-5.
  • Smith E, Dombrovsky A 2020. Aspects in Tobamovirus Management in Intensive Agriculture. In Plant Diseases - Current Threats and Management Trends. IntechOpen. https://doi.org/10.5772/ intechopen.87101.
  • Solis I, Garcia-Arenal F 1990. The complete nucleotide sequence of the genomic RNA of the tobamovirus tobacco mild green mosaic virus. Virology, 177(2):553-8. doi: 10.1016/0042-6822(90)90520-2. PMID: 2371769.
  • Thompson JD, Higgins DG, Gibson TJ 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weigh matrix choice. Nucleic Acid Research, 22:4673-4680.

Türkiye'de Biberde Enfeksiyon Oluşturan Tobacco mild green mosaic virus İzolatlarının Belirlenmesi ve Moleküler Karakterizasyonu

Year 2022, Volume: 25 Issue: Ek Sayı 2, 490 - 496, 30.12.2022

Selin Ceren Balsak Seher Gül Avcu Nihal Buzkan

https://doi.org/10.18016/ksutarimdoga.vi.1048580

Abstract

Bu çalışmada, Türkiye’de biber yetiştiriciliğinin ekonomik olarak önemli olduğu iki bölgede Tobacco mild green mosaic virus (TMGMV) enfeksiyonu incelenmiştir. Mozaik, sararma, beneklenme ve çukurlaşma simptomu gösteren toplam 397 yaprak ve meyve örneği toplanmış ve double antibody sandwich ELISA (DAS-ELISA) ile poliklonal antiserum kullanılarak testlenmiştir. DAS-ELISA testinde testlenen örnekler içerisinde 97 örneğin (%24.4) TMGMV enfeksiyonuna sahip olduğu belirlenmiştir. TMGMV ile enfekteli pozitif örnekler, gen spesifik primerler kullanılarak RT-PCR yöntemiyle virüs kılıf protein (KP) geninin çoğaltılması ve izolatların moleküler karakterizasyonu için kullanılmıştır. KP nükleotid dizilerinin karşılaştırması sonucunda, izolatların kendi aralarında nükleotid benzerliğinin %97.2-100, amino asit benzerliğinin ise %96.8-100 arasında değiştiği belirlenmiştir. Bildiğimiz kadarıyla, bu Türkiye'de TMGMV’nin enfeksiyonunun Capsicum annuum'daki ilk raporudur.

Keywords

Tobamovirus TMGMV Capsicum annuum DAS-ELISA Kılıf protein

Project Number

2021/1-15YLS

References

  • Alonso E, Garcı´a-Luque I, Avila-Rinco´n MJ, Wicke B, Serra MT, Dı´az-Ruı´z JR 1989. A tobamovirus causing heavy losses in protected pepper crops in Spain. Journal of Phytopathology, 125(1):67–76.
  • Broadbent L 1965. The epidemiology of tomato mosaic. XI. Seed transmission of TMV. The Annals of Applied Biology, 56(2):177-205.
  • Buzkan N, Arpacı BB 2017. New emerging viruses in pepper crops in Turkey. 15th Congress of the Mediterranean Phytopathological Union, June 20-23, 2017, Cordoba, Spain.
  • Çağlar B, Fidan KH, Elbeaino T 2013. Detection and Molecular Characterization of Pepper Mild Mottle Virus from Turkey. Journal of Phytopathology, 161 (6): 434-438.
  • Chomczynski P, Sacchi N 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry, 162(1):156-9. doi: 10.1006/abio.1987. 9999. PMID: 2440339.
  • Clark MF, Adams AN 1977. Characteristic of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology, 34(3): 475–483.
  • Faostat 2020. Food And Agriculture Organization. Statistical Databases Web Page (www.faostat.fao .org).
  • Fidan H, Sarikaya P, Yildiz K, Topkaya B, Erkis G, Calis O 2021. Robust molecular detection of the new Tomato brown rugose fruit virus in infected tomato and pepper plants from Turkey, Journal of Integrative Agriculture, 20(8): 2170-2179.
  • Green SK, Kim JS 1994. Source of resistance to viruses of pepper (Capsicum spp.): A catalog. Technical Bulletin, 20, AVRDC, 64.
  • Jarret RL, Gillaspie AG, Barkley NA, Pinnow DL 2008. The occurrence and control of pepper mild mottle virus (PMMoV) in the USDA/ARS. Capsicum germplasm collection. Seed technology, 26-36. https://naldc.nal.usda.gov/download/26970/PDF.
  • Karanfil A, Sarı M, Korkmaz S 2020. First report of tobacco mild green mosaic virus in Turkey. Journal of Plant Pathology, 102, 547.
  • Kenyon L, Kumar S, Tsai WS, Hughes JD 2014. Virus diseases of peppers (Capsicum spp.) and their control. Advances in Virus Research, 90:297‐354. doi:10.1016/B978-0-12-801246-8.00006-8.
  • Kumar S, Stecher G, Tamura K 2016. Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7):1870–1874.
  • Lanter JM, McGuire JM, Goode M 1982. Persistence of tomato mosaic virus in tomato debris and soil under field conditions. Plant Disease, 66(7): 552-555.
  • Letschert B, Adam G, Lesemann DE, Willingmann P, Heinze C 2002. Detection and differentiation of serologically crossreacting tobamoviruses of economical importance by RTPCR and RT-PCR-RFLP. Journal of Virological Methods, 106(1):1–10.
  • Moury B, Morel C, Johansen E, Guilbaud L, Souche S, Ayme V, Caranta C, Palloix A, Jacquemond M. 2004. Mutations in potato virus Y genome-linked protein determine virulence toward recessive resistances in Capsicum annuum and Lycopersicon hirsutum. Molecular Plant-Microbe Interaction, 17(3):322-9. doi: 10.1094/MPMI. 2004.17.3.322. PMID: 15000399.
  • Moury B, Verdin E 2012. Viruses of pepper crops in the Mediterranean basin: a remarkable stasis. Advances in Virus Research, 84: 127–162.
  • Ozaslan M, Bas B, Aytekin T, Sigirci Z 2006. Identification of Pepper Viruses by Das-elisa Assays in Gaziantep-Turkey. Plant Pathology Journal, 5(1):11-14.
  • Pares RD, Gunn LV, Keskula EN 1996. The role of infective plant debris, and its concentration in soil, in the ecology of tomato mosaic tobamovirus—A non-vectored plant virus. Journal of Phytopathology-Phytopathologische Zeitschrift, 144(3):147-150.
  • Pernezny KL, Roberts PD, Murphy JF, Goldberg NP 2003. Compendium of pepper diseases. St. Paul, MN: APS Press.
  • Rast ATB, Stijger CCMM 1987. Disinfection of pepper seed infected with different strains of capsicum mosaic virus by trisodium phosphate and dry heat treatment. Plant Pathology, 36(4): 583–588.
  • Salamon P, Kaszta M 2000. Investigation on the transmission of some Tobamoviruses by pollen and seed in pepper (Capsicum annuum L.). In: 8 The International Pollination Symposium, Mosonmagyarovar, Hungary, 10–14 July, 2000. International Journal of Horticultural Science, 6: 127 –131.
  • Şevik MA 2011. Occurrence of pepper mild mottle virus in greenhouse grown pepper (Capsicum annuum L.) in the Test Mediterranean region of Turkey. African Journal of Biotechnology, 10(25): 4976-4979.
  • Şimşek D, Pınar H, Mutlu N 2015. Moleküler Islah Yöntemleri Kullanılarak Tospovirus ve Tobamoviruslere Dayanıklı Çarli Biber (Capsicum annum L.) Hat ve Çeşitlerinin Geliştirilmesi. Selçuk Tarım Bilimleri Dergisi, 1(1):1-5.
  • Smith E, Dombrovsky A 2020. Aspects in Tobamovirus Management in Intensive Agriculture. In Plant Diseases - Current Threats and Management Trends. IntechOpen. https://doi.org/10.5772/ intechopen.87101.
  • Solis I, Garcia-Arenal F 1990. The complete nucleotide sequence of the genomic RNA of the tobamovirus tobacco mild green mosaic virus. Virology, 177(2):553-8. doi: 10.1016/0042-6822(90)90520-2. PMID: 2371769.
  • Thompson JD, Higgins DG, Gibson TJ 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weigh matrix choice. Nucleic Acid Research, 22:4673-4680.
There are 27 citations in total.

Details

Primary Language English
Subjects Agricultural, Veterinary and Food Sciences
Journal Section RESEARCH ARTICLE
Authors

Selin Ceren Balsak 0000-0002-2326-7520

Seher Gül Avcu 0000-0002-5499-6611

Nihal Buzkan 0000-0002-0428-0447

Project Number 2021/1-15YLS
Publication Date December 30, 2022
Submission Date December 27, 2021
Acceptance Date June 12, 2022
Published in Issue Year 2022Volume: 25 Issue: Ek Sayı 2

Cite

APA Balsak, S. C., Avcu, S. G., & Buzkan, N. (2022). Detection and Molecular Characterization of Tobacco mild green mosaic virus Isolates Infecting Peppers in Turkey. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(Ek Sayı 2), 490-496. https://doi.org/10.18016/ksutarimdoga.vi.1048580
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