Research Article
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Evaluation of In-vitro Anticandidal Activity of 99 Different Commercial Plant Extract, Fixed and Essential Oils against Vaginal Candida albicans Isolates

Year 2023, Volume: 26 Issue: 5, 1034 - 1047, 31.10.2023

Gülçin Özcan Ateş Savaş Kanbur

https://doi.org/10.18016/ksutarimdoga.vi.1211862
Cited By: 2

Abstract

Plant extracts (PE), fixed oils (FO) and essential oils (EO) are used in traditional medicine to treat various diseases. This study evaluated the anticandidal activity of 100 different commercially available PEs, FOs, and EOs against 19 Candida albicans vaginal isolates and C. albicans ATCC 10231. It was determined that 20 EOs and FOs had anticandidal activity. Piper nigrum FO, pine turpentine EO, pine tar EO, and Eugenia caryophyllata EO showed the highest anticandidal activity. The minimum inhibitory concentrations (MIC) and minimum fungicidal concentrations (MFC) values of these FO and EOs were between 0.125 and 2 µL mL-1. The volatile components of these FOs and EOs were determined by GC-MS analysis. There were six components in E. caryophyllata EO, 38 in Pine turpentine EO, 39 in P. nigrum FO, and 119 in Pine tar EO. In conclusion, this FOs and EOs can be used to treat vulvovaginal candidiasis.

Keywords

Anticandidal activity Plant fixed oil Essential oil Vaginal Candida

Supporting Institution

Çanakkale Onsekiz Mart Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

THD-2020-3490

Thanks

This study was conducted in Çanakkale Onsekiz Mart University Experimental Research Center (ÇOMÜDAM) and Çanakkale Onsekiz Mart University, Faculty of Medicine, Medical Microbiology Laboratory. We are grateful to Prof. Dr. Müşerref OTKUN for Medical Microbiology Laboratory use.

References

  • Asmerom, D., Kalay, T.H., & Tafere, G.G. (2020). Antibacterial and Antifungal Activities of the Leaf Exudate of Aloe megalacantha Baker. International Journal of Microbiology, 2020, 1-6.
  • CDC Drug-Resistant Candida Species. https://www. cdc.gov/drugresistance/pdf/threats-report/candida-508.pdf. (Date accessed: 09.03.2022).
  • Gatsing, D., Tchakoute, V., Ngamga, D., Kuiate, J.R., Tamokou, J. D. D., Nji Nkah, B. F., Tchouanguep, F.M., & Fodouop, S.P.C. (2009). In Vitro Antibacterial activity of Crinum Purpurascens herb leaf extract against the Salmonella species causing typhoid fever and its toxicological evaluation. Iran J Med Sci, 37(2), 126-136.
  • Ghaddar, N., Anastasiadis, E., Halimeh, R., Ghaddar, A., Dhar, R., AlFouzan, W., ... & El Chaar, M. (2020). Prevalence and antifungal susceptibility of Candida albicans causing vaginal discharge among pregnant women in Lebanon. BMC infectious diseases, 20(1), 1-9.
  • Ghaffari, T., Kafil, H.S., Asnaashari, S., Farajnia, S., Delazar, A., Baek, S.C., ... & Kim, K.H. (2019). Chemical composition and antimicrobial activity of essential oils from the aerial parts of Pinus eldarica grown in Northwestern Iran. Molecules, 24(17), 3203.
  • Gonçalves, B., Ferreira, C., Alves, C.T., Henriques, M., Azeredo, J., & Silva, S. (2016). Vulvovaginal candidiasis: Epidemiology, microbiology and risk factors. Critical reviews in microbiology, 42(6), 905-927.
  • Gülçin, İ., Büyükokuroǧlu, M.E., Oktay, M., & Küfrevioǧlu, Ö.İ. (2003). Antioxidant and analgesic activities of turpentine of Pinus nigra Arn. subsp. pallsiana (Lamb.) Holmboe. Journal of Ethnopharmacology, 86(1), 51-58.
  • Hamidpour, R., Hamidpour, S., Hamidpour, M., Shahlari, M., Sohraby, M., Shahlari, N., & Hamidpour, R. (2017). Russian olive (Elaeagnus angustifolia L.): From a variety of traditional medicinal applications to its novel roles as active antioxidant, anti-inflammatory, anti-mutagenic and analgesic agent. Journal of traditional and complementary medicine, 7(1), 24-29.
  • Hammer, K.A., Carson, C.F., & Riley, T.V. (1999). Antimicrobial activity of essential oils and other plant extracts. Journal of applied microbiology, 86(6), 985-990.
  • Hassan, A. & Amjid, I. (2009). Gas chromatography-mass spectrometric studies of stems essential oil of Pinus roxburghaii and their antibacterial and antifungal activities. Journal of Medicinal Plants Research, 3(9), 670-673.
  • Ibišević, M., Pilipović, S., Nešić, I., Kerleta, V., Husejnagić, D., Kozarević, E. C., ... & Karić, E. (2020). Antimicrobial activity of liposomal and non-liposomal vaginal suppositories with Origanum compactum essential oil. Technologica Acta-Scientific/professional journal of chemistry and technology, 13(2), 5-10.
  • Incilay, G. (2014). Volatile Composition, Antimicrobial and Antioxidant Properties of Different Parts from Elaeagnus angustifolia L. Journal of Essential Oil Bearing Plants, 17(6), 1187-1202.
  • Kola-Mustapha, A.T., Bamigboye, F.A., Olufadi-Ahmed, H.Y., Ayotunde, H.T., & Ghazali, Y.O. (2021). Evaluation and Formulation of the Methanol Extract and Oil of Nigella sativa Linn into Suppositories with Potentials in the Management of Vaginal Candidiasis. Current Traditional Medicine, 7(4), 606-614.
  • Kurti, F., Giorgi, A., Beretta, G., Mustafa, B., Gelmini, F., Testa, C., ... & Hajdari, A. (2019). Chemical composition, antioxidant and antimicrobial activities of essential oils of different Pinus species from Kosovo. Journal of Essential Oil Research, 31(4), 263-275.
  • Lang, G. & Buchbauer, G. (2012). A review on recent research results (2008–2010) on essential oils as antimicrobials and antifungals. A review. Flavour Fragr J, 27, 13–39.
  • Mandal, M.D. & Mandal, S. (2011). Honey: its medicinal property and antibacterial activity. Asian Pacific journal of tropical biomedicine, 1(2), 154-160.
  • Mansourian, A., Boojarpour, N., Ashnagar, S., Beitollahi, J.M., & Shamshiri, A.R. (2014). The comparative study of antifungal activity of Syzygium aromaticum, Punica granatum and nystatin on Candida albicans; an in vitro study. Journal de mycologie medicale, 24(4), e163-e168.
  • Mittal, M., Gupta, N., Parashar, P., Mehra, V., & Khatri, M. (2014). Phytochemical evaluation and pharmacological activity of Syzygium aromaticum: a comprehensive review. International Journal of Pharmacy and Pharmaceutical Sciences, 6(8), 67-72.
  • Monjazeb Marvdashti, L., Mohammadbeigi, M., Arab, S., Ebrahimi, A., Rezaei, A., & Abdolshahi, A. (2023). Elaeagnus angustifolia L. Whole Fruit Ethanolic Extract: Phytochemical Composition and Antimicrobial Effects. Journal of Chemical Health Risks (JCHR), 13(0), (InPress). https://doi.org/ 10.22034/ jchr.2022.1899059.1130
  • Mseddi, K., Alimi, F., Noumi, E., Veettil, V. N., Deshpande, S., Adnan, M., Hamdi, A., Elkahoui, S., Alghamdi, A., Kadri, A., Patel, M., & Snoussi, M. (2020). Thymus musilii Velen. as a promising source of potent bioactive compounds with its pharmacological properties: In vitro and in silico analysis. Arabian Journal of Chemistry, 13(8), 6782-6801.
  • Nalbantbaşi, Z., & Gölcü, A. (2009). Kahramanmaras Yöresine Ait Sifalı Bitkilerin Antimikrobiyal Aktiviteleri. KSÜ Doğa Bilimleri Dergisi, 12(2), 1-8.
  • Okmen, G. & Turkcan, O. (2014). A study on antimicrobial, antioxidant and antimutagenic activities of Elaeagnus angustifolia L. leaves. African Journal of Traditional, Complementary and Alternative Medicines, 11(1), 116-120.
  • Permana, A.D., Utomo, E., Pratama, M.R., Amir, M.N., Anjani, Q.K., Mardikasari, S.A., ... & Donnelly, R.F. (2021). Bioadhesive-Thermosensitive In Situ Vaginal Gel of the Gel Flake-Solid Dispersion of Itraconazole for Enhanced Antifungal Activity in the Treatment of Vaginal Candidiasis. ACS applied materials & interfaces, 13(15), 18128-18141.
  • Pinto, E., Vale-Silva, L., Cavaleiro, C., & Salgueiro, L. (2009). Antifungal activity of the clove essential oil from Syzygium aromaticum on Candida, Aspergillus and dermatophyte species. Journal of medical microbiology, 58(11), 1454-1462.
  • Rana, I.S., Rana, A.S., & Rajak, R.C. (2011). Evaluation of antifungal activity in essential oil of the Syzygium aromaticum (L.) by extraction, purification and analysis of its main component eugenol. Brazilian Journal of Microbiology, 42, 1269-1277.
  • Ranasinghe, L., Jayawardena, B., & Abeywickrama, K. (2002). Fungicidal activity of essential oils of Cinnamomum zeylanicum (L.) and Syzygium aromaticum (L.) Merr et LM Perry against crown rot and anthracnose pathogens isolated from banana. Letters in Applied Microbiology, 35(3), 208-211.
  • Snoussi, M., Noumi, E., Punchappady-Devasya, R., Trabelsi, N., Kanekar, S., Nazzaro, F., Fratianni, F., Flamini, G., De Feo, V., & Al-Sieni, A. (2018). Antioxidant properties and anti-quorum sensing potential of Carum copticum essential oil and phenolics against Chromobacterium violaceum. Journal of food science and technology, 55(8), 2824-2832.
  • Tsega, A., & Mekonnen, F. (2019). Prevalence, risk factors and antifungal susceptibility pattern of Candida species among pregnant women at Debre Markos Referral Hospital, Northwest Ethiopia. BMC pregnancy and childbirth, 19(1), 1-8.
  • Tümen, İ., & Reunanen, M. (2010). A comparative study on turpentine oils of oleoresins of Pinus sylvestris L. from three districts of Denizli. Records of Natural Products, 4(4), 224.
  • Yassin, M. T., Mostafa, A. A. F., & Al-Askar, A. A. (2020). In vitro anticandidal potency of Syzygium aromaticum (clove) extracts against vaginal candidiasis. BMC complementary medicine and therapies, 20(1), 1-9.

99 Farklı Ticari Bitki Ekstrakt, Sabit ve Uçucu Yağın Vajinal Candida albicans İzolatlarına Karşı In-vitro Antikandidal Aktivitesinin Değerlendirilmesi

Year 2023, Volume: 26 Issue: 5, 1034 - 1047, 31.10.2023

Gülçin Özcan Ateş Savaş Kanbur

https://doi.org/10.18016/ksutarimdoga.vi.1211862
Cited By: 2

Abstract

Bitki ekstrakt (PE), sabit yağları (FO) ve esansiyel yağları (EO) çeşitli rahatsızlıkların tedavisinde geleneksel tıpta kullanılmaktadır. Bu nedenle bu çalışmada ticari olarak satışı bulunan 99 adet PE, FO ve EO’nun 19 Candida albicans vajinal izolatına ve C. albicans ATCC 10231 standart kültürüne karşı antikandidal aktivitesi değerlendirilmiştir. 20 adet PE, FO ve EO’nun antikandidal aktiviteye sahip olduğu belirlendi. En yüksek antikandidal aktiviteyi ise Piper nigrum FO, pine turpentine EO, pine tar EO ve Eugenia caryophyllata EO gösterdiği belirlenmiştir. Bu FO ve EO’ların minimum inhibitör konsantrasyonu (MİK) ve minimum fungisidal konsantrasyonu (MFC) değerlerinin ise en düşük 0.125 µL mL-1 ve en yüksek ise 2 µL mL-1 olduğu belirlenmiştir. Bu yağların uçucu bileşenleri ise GC-MS analizi ile belirlenmiştir. Karanfil yağında 6 bileşen, çam terebentin yağında 38 bileşen, karabiber yağında 39 bileşen ve çam katranın da ise 119 bileşen tanımlanmıştır. Sonuç olarak yeni antifungal bileşenlerin belirlenmesine katkı sağlayacak bitki esansiyel yağları belirlenmiştir.

Keywords

Antikandidal aktivite Bitki sabit yağ Esansiyel yağ Vajinal Candida

Project Number

THD-2020-3490

References

  • Asmerom, D., Kalay, T.H., & Tafere, G.G. (2020). Antibacterial and Antifungal Activities of the Leaf Exudate of Aloe megalacantha Baker. International Journal of Microbiology, 2020, 1-6.
  • CDC Drug-Resistant Candida Species. https://www. cdc.gov/drugresistance/pdf/threats-report/candida-508.pdf. (Date accessed: 09.03.2022).
  • Gatsing, D., Tchakoute, V., Ngamga, D., Kuiate, J.R., Tamokou, J. D. D., Nji Nkah, B. F., Tchouanguep, F.M., & Fodouop, S.P.C. (2009). In Vitro Antibacterial activity of Crinum Purpurascens herb leaf extract against the Salmonella species causing typhoid fever and its toxicological evaluation. Iran J Med Sci, 37(2), 126-136.
  • Ghaddar, N., Anastasiadis, E., Halimeh, R., Ghaddar, A., Dhar, R., AlFouzan, W., ... & El Chaar, M. (2020). Prevalence and antifungal susceptibility of Candida albicans causing vaginal discharge among pregnant women in Lebanon. BMC infectious diseases, 20(1), 1-9.
  • Ghaffari, T., Kafil, H.S., Asnaashari, S., Farajnia, S., Delazar, A., Baek, S.C., ... & Kim, K.H. (2019). Chemical composition and antimicrobial activity of essential oils from the aerial parts of Pinus eldarica grown in Northwestern Iran. Molecules, 24(17), 3203.
  • Gonçalves, B., Ferreira, C., Alves, C.T., Henriques, M., Azeredo, J., & Silva, S. (2016). Vulvovaginal candidiasis: Epidemiology, microbiology and risk factors. Critical reviews in microbiology, 42(6), 905-927.
  • Gülçin, İ., Büyükokuroǧlu, M.E., Oktay, M., & Küfrevioǧlu, Ö.İ. (2003). Antioxidant and analgesic activities of turpentine of Pinus nigra Arn. subsp. pallsiana (Lamb.) Holmboe. Journal of Ethnopharmacology, 86(1), 51-58.
  • Hamidpour, R., Hamidpour, S., Hamidpour, M., Shahlari, M., Sohraby, M., Shahlari, N., & Hamidpour, R. (2017). Russian olive (Elaeagnus angustifolia L.): From a variety of traditional medicinal applications to its novel roles as active antioxidant, anti-inflammatory, anti-mutagenic and analgesic agent. Journal of traditional and complementary medicine, 7(1), 24-29.
  • Hammer, K.A., Carson, C.F., & Riley, T.V. (1999). Antimicrobial activity of essential oils and other plant extracts. Journal of applied microbiology, 86(6), 985-990.
  • Hassan, A. & Amjid, I. (2009). Gas chromatography-mass spectrometric studies of stems essential oil of Pinus roxburghaii and their antibacterial and antifungal activities. Journal of Medicinal Plants Research, 3(9), 670-673.
  • Ibišević, M., Pilipović, S., Nešić, I., Kerleta, V., Husejnagić, D., Kozarević, E. C., ... & Karić, E. (2020). Antimicrobial activity of liposomal and non-liposomal vaginal suppositories with Origanum compactum essential oil. Technologica Acta-Scientific/professional journal of chemistry and technology, 13(2), 5-10.
  • Incilay, G. (2014). Volatile Composition, Antimicrobial and Antioxidant Properties of Different Parts from Elaeagnus angustifolia L. Journal of Essential Oil Bearing Plants, 17(6), 1187-1202.
  • Kola-Mustapha, A.T., Bamigboye, F.A., Olufadi-Ahmed, H.Y., Ayotunde, H.T., & Ghazali, Y.O. (2021). Evaluation and Formulation of the Methanol Extract and Oil of Nigella sativa Linn into Suppositories with Potentials in the Management of Vaginal Candidiasis. Current Traditional Medicine, 7(4), 606-614.
  • Kurti, F., Giorgi, A., Beretta, G., Mustafa, B., Gelmini, F., Testa, C., ... & Hajdari, A. (2019). Chemical composition, antioxidant and antimicrobial activities of essential oils of different Pinus species from Kosovo. Journal of Essential Oil Research, 31(4), 263-275.
  • Lang, G. & Buchbauer, G. (2012). A review on recent research results (2008–2010) on essential oils as antimicrobials and antifungals. A review. Flavour Fragr J, 27, 13–39.
  • Mandal, M.D. & Mandal, S. (2011). Honey: its medicinal property and antibacterial activity. Asian Pacific journal of tropical biomedicine, 1(2), 154-160.
  • Mansourian, A., Boojarpour, N., Ashnagar, S., Beitollahi, J.M., & Shamshiri, A.R. (2014). The comparative study of antifungal activity of Syzygium aromaticum, Punica granatum and nystatin on Candida albicans; an in vitro study. Journal de mycologie medicale, 24(4), e163-e168.
  • Mittal, M., Gupta, N., Parashar, P., Mehra, V., & Khatri, M. (2014). Phytochemical evaluation and pharmacological activity of Syzygium aromaticum: a comprehensive review. International Journal of Pharmacy and Pharmaceutical Sciences, 6(8), 67-72.
  • Monjazeb Marvdashti, L., Mohammadbeigi, M., Arab, S., Ebrahimi, A., Rezaei, A., & Abdolshahi, A. (2023). Elaeagnus angustifolia L. Whole Fruit Ethanolic Extract: Phytochemical Composition and Antimicrobial Effects. Journal of Chemical Health Risks (JCHR), 13(0), (InPress). https://doi.org/ 10.22034/ jchr.2022.1899059.1130
  • Mseddi, K., Alimi, F., Noumi, E., Veettil, V. N., Deshpande, S., Adnan, M., Hamdi, A., Elkahoui, S., Alghamdi, A., Kadri, A., Patel, M., & Snoussi, M. (2020). Thymus musilii Velen. as a promising source of potent bioactive compounds with its pharmacological properties: In vitro and in silico analysis. Arabian Journal of Chemistry, 13(8), 6782-6801.
  • Nalbantbaşi, Z., & Gölcü, A. (2009). Kahramanmaras Yöresine Ait Sifalı Bitkilerin Antimikrobiyal Aktiviteleri. KSÜ Doğa Bilimleri Dergisi, 12(2), 1-8.
  • Okmen, G. & Turkcan, O. (2014). A study on antimicrobial, antioxidant and antimutagenic activities of Elaeagnus angustifolia L. leaves. African Journal of Traditional, Complementary and Alternative Medicines, 11(1), 116-120.
  • Permana, A.D., Utomo, E., Pratama, M.R., Amir, M.N., Anjani, Q.K., Mardikasari, S.A., ... & Donnelly, R.F. (2021). Bioadhesive-Thermosensitive In Situ Vaginal Gel of the Gel Flake-Solid Dispersion of Itraconazole for Enhanced Antifungal Activity in the Treatment of Vaginal Candidiasis. ACS applied materials & interfaces, 13(15), 18128-18141.
  • Pinto, E., Vale-Silva, L., Cavaleiro, C., & Salgueiro, L. (2009). Antifungal activity of the clove essential oil from Syzygium aromaticum on Candida, Aspergillus and dermatophyte species. Journal of medical microbiology, 58(11), 1454-1462.
  • Rana, I.S., Rana, A.S., & Rajak, R.C. (2011). Evaluation of antifungal activity in essential oil of the Syzygium aromaticum (L.) by extraction, purification and analysis of its main component eugenol. Brazilian Journal of Microbiology, 42, 1269-1277.
  • Ranasinghe, L., Jayawardena, B., & Abeywickrama, K. (2002). Fungicidal activity of essential oils of Cinnamomum zeylanicum (L.) and Syzygium aromaticum (L.) Merr et LM Perry against crown rot and anthracnose pathogens isolated from banana. Letters in Applied Microbiology, 35(3), 208-211.
  • Snoussi, M., Noumi, E., Punchappady-Devasya, R., Trabelsi, N., Kanekar, S., Nazzaro, F., Fratianni, F., Flamini, G., De Feo, V., & Al-Sieni, A. (2018). Antioxidant properties and anti-quorum sensing potential of Carum copticum essential oil and phenolics against Chromobacterium violaceum. Journal of food science and technology, 55(8), 2824-2832.
  • Tsega, A., & Mekonnen, F. (2019). Prevalence, risk factors and antifungal susceptibility pattern of Candida species among pregnant women at Debre Markos Referral Hospital, Northwest Ethiopia. BMC pregnancy and childbirth, 19(1), 1-8.
  • Tümen, İ., & Reunanen, M. (2010). A comparative study on turpentine oils of oleoresins of Pinus sylvestris L. from three districts of Denizli. Records of Natural Products, 4(4), 224.
  • Yassin, M. T., Mostafa, A. A. F., & Al-Askar, A. A. (2020). In vitro anticandidal potency of Syzygium aromaticum (clove) extracts against vaginal candidiasis. BMC complementary medicine and therapies, 20(1), 1-9.
There are 30 citations in total.

Details

Primary Language English
Journal Section RESEARCH ARTICLE
Authors

Gülçin Özcan Ateş 0000-0002-8467-2378

Savaş Kanbur 0000-0002-8770-0194

Project Number THD-2020-3490
Early Pub Date May 27, 2023
Publication Date October 31, 2023
Submission Date November 30, 2022
Acceptance Date March 23, 2023
Published in Issue Year 2023Volume: 26 Issue: 5

Cite

APA Özcan Ateş, G., & Kanbur, S. (2023). Evaluation of In-vitro Anticandidal Activity of 99 Different Commercial Plant Extract, Fixed and Essential Oils against Vaginal Candida albicans Isolates. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(5), 1034-1047. https://doi.org/10.18016/ksutarimdoga.vi.1211862

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https://doi.org/10.29133/yyutbd.1403366
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