Research Article
BibTex RIS Cite

Green Synthesis and Antibiofilm Activity of Silver Nanoparticles by Camellia sinensis L. (White Tea Leaf)

Year 2024, Volume: 27 Issue: 2, 285 - 292, 01.04.2024

İlke Karakaş Nurcihan Hacıoğlu Bahri Emirhan Özdemir

https://doi.org/10.18016/ksutarimdoga.vi.1297130

Abstract

Biosynthesis of nanoparticles refers to the production or synthesis of nanoparticles using organisms, such as bacteria, fungi, plants or their byproducts. This approach offers several advantages over conventional chemical methods, including eco-friendliness, cost-effectiveness, and potential for large-scale production. The silver nanoparticles (AgNPs) synthesized using aqueous Camellia sinensis L. (white tea leaf) extracts as reducing and stabilizing agents were reported and evaluated for antibiofilm activity against test microorganisms (Acinetobacter baumanii ATCC 19606 NRRLB 3704, Pseudomonas aeruginosa ATCC 27853 (Gram –), and Bacillus subtilis ATCC 6633, Staphylococcus haemolyticus ATCC 43252 (Gram +), and Candida albicans ATCC 10231) in the study. The synthesized AgNPs were observed and characterized using Uv-Vis spectroscopic analysis, scanning electron and transmission electron microscopy energy-dispersive spectra and Fourier transform infrared spectroscopy. The synthesized AgNP was also screened for antibiofilm activity against test microorganisms. Our results show that the synthesized AgNPs have the potential to be used for antibiofilm materials and different biomedical applications.

Keywords

Camellia sinensis L. Silver Nanoparticle Antibiofilm Activity

Supporting Institution

Tübitak

Project Number

2209-A

Thanks

This research is a part of Mr. Bahri Emirhan ÖZDEMİR’s TÜBİTAK-2209 A Project which is supported with the frame of Undergraduate Students Grant in Biological Science.

References

  • Atalar, M.N., Baran, A., Baran, M.F., Keskin, C., Aktepe, N., Yavuz, Ö., & İrtegun-Kandemir, S. (2022). Economic fast synthesis of olive leaf extract and silver nanoparticles and biomedical applications. Particulate Science and Technology, 40(5), 589-597.
  • Azizi, S., Shahri, M.M., Rahman, H.S., Rahim, R.A., Rasedee, A., & Mohamad, R., (2017). Green synthesis palladium nanoparticles mediated by white tea (Camellia sinensis) extract with antioxidants, antibacterial and antiproliferative activities towards the human leukaemia (MOLT-4) cell line. International Journal of Nanomedicine, 12, 8841-8853.
  • Baran, M.F. (2019). Synthesis and antimicrobial applications of silver nanoparticles from Artemisia absinthium plant. Biological and Chemical Research, 6, 96-103.
  • Barry, A.L., Craig, W.A., Nadler, H., Barth Reller, L., Sanders, C.C,, & Swenson, J.M., (1999). Methods for determining bactericidal activity of antimicrobial agents; approved guideline (4. edition), 1999, USA, Clinical and Laboratory Standards Institute.
  • Bayğu, G., (2020). Cimin üzümü yaprağı kullanılarak yeşil sentez yöntemiyle elde edilen gümüş nanopartikülünün genotoksik etkisinin kanat benek testi ile belirlenmesi. Master thesis, Erzincan Binali Yıldırım University.
  • Chandran, S.P., Chaudhary, M., Pasricha, R., Ahmad, A., & Sastry, M., (2006). Synthesis of gold nanotriangles and silver nanoparticles using Aloevera plant extract. Biotechnology progress, 22, 577-583.
  • El-Taher, E.M., (2011). Kombucha: A new microbial phenomenon and industrial benefits. African Journal of Biological Sciences, 7(2), 41-60.
  • Gan, P.P., & Li, S.F.Y., (2012). Potential of plant as a biological factory to synthesize gold and silver nanoparticles and their applications. Reviews in Environmental Science and Bio/Technology, 11(2), 169-206.
  • Ghaffari-Moghaddam, M., & Hadi-Dabanlou, R., (2014). Plant mediated green synthesis and antibacterial activity of silver nanoparticles using Crataegus douglasii fruit extract. Journal of Industrial and Engineering Chemistry, 20(2), 739-744.
  • Göl, F., Aygün, A., Seyrankaya, A., Gür, T., Yenikaya, C., & Şen, F., (2020). Green synthesis and characterization of Camellia sinensis mediated silver nanoparticles for antibacterial ceramic applications. Materials Chemistry and Physics, 250, 123-137.
  • Göse, M., & Hacıoğlu-Doğru, N., (2021). Bioactive compounds, antimicrobial and antibiofilm activity of two Verbascum species. KSÜ Tarım ve Doğa Dergisi 24(3), 479-487.
  • Hodoroaba, V., (2020). Energy-dispersive X-ray spectroscopy (EDS) Characterization of Nanoparticles, 397-417.
  • Ilgaz, A.Ş., Kalcıoğlu, Z., & İslamoğlu, E., (2006). Türk beyaz çayı üretim yönetiminin optimizasyonu ve Türk beyaz çayının kalite parametrelerinin belirlenmesi. Çaykur Çay İşletmeleri Genel Müdürlüğü Atatürk Çay ve Bahçe Kültürleri Araştırma Enstitüsü Teknoloji Kısım Müdürlüğü, 1-37.
  • İpek, İ., Baran, M.F., Baran, A., Hatipoğlu, A., Keskin, C., Yildiztekin, M., Küçükaydin, S., Becerekli, H., Kurt, K., Eftekhari, A., Huseynova, I., Khalilov, R., & Cho, W.C. (2023). Green synthesis and evaluation of antipathogenic, antioxidant, and anticholinesterase activities of gold nanoparticles (Au NPs) from Allium cepa L. peel aqueous extract. Biomass Conversion and Biorefnery, DOI: 10.1007/s13399-023-04362-y
  • Kouvaris, P., Delimitis, A., Zaspalis, V., Papadopoulos, D., Tsipas, S.A., & Michailidis, N., (2012). Green synthesis and characterization of silver nanoparticles produced using Arbutus unedo leaf extract. Materials Letters, 76, 18-20.
  • Kumar, S.P., Darshit, P., Ankita, P., Palak, D., Ram, P., Pradip, P., & Kaliaperumal, S., (2011). Biogenic synthesis of silver nanoparticles using Nicotiana tobaccum leaf extract and study of their antibacterial effect. African Journal of Biotechnology, 10(41), 8122-8130.
  • Merritt, J.H., Kadouri, D.E., & O’Toole, G.A., (2005). Growing and analyzing static biofilms. Current Protocol Microbiology,1(1B), 853.
  • Mohanta, B., Chakraborty, A., Selvaraj, S., & Roy, A., (2020). Bactericidal effect of gentamicin conjugated gold nanoparticles. Micro & Nano Letters, 15(10), 640-716.
  • Qi, P., Zhang, D., Zeng, Y., & Wan, Y., (2016). Biosynthesis of CdS nanoparticles: A fluorescent sensor for sulfate-reducing bacteria detection. Talanta, 147, 142-146.
  • Pallela, P.N.V.K., Ummey, S., Ruddaraju, L.K., Pammi, S.V.N., & Yoon, S.G., (2018). Ultra Small, mono dispersed green synthesized silver nanoparticles using aqueous extract of Sida cordifolia plant and investigation of antibacterial activity. Microbial Pathogenesis, 124, 63-69.
  • Salah, N., Miller, N.J., Paganga, G., Tijburg, L., Bolwell, G.P., & Rice-Evans, C., (1995). Polyphenolic flavanols as scavengers of aqueous phase radicals and as chain- breaking antioxidants. Archives of Biochemistry and Biophysics, 22, 339-346.
  • Składanowski, M., Wypij, M., Laskowski, D., Golińska, P., Dahm, H., & Rai, M., (2017). Silver and gold nanoparticles synthesized from Streptomyces sp. isolated from acid forest soil With special reference to its antibacterial activity against pathogens. Journal of Cluster Science, 28(1), 59-79.
  • Tamuly, C., Hazarika, M., Borah, S.C., Das, M.R., & Boruah, M.P., (2013). In situ biosynthesis of Ag, Au and bimetallic nanoparticles using Piper pedicellatum C.DC: green chemistry approach. Colloids and surfaces B: Biointerfaces, 102, 627-634.
  • Umaz, A. , Koç, A. , Baran, M.F. , Keskin, C. & Atalar, M.N. (2019). Hypericum Triquetrifolium Turra bitkisinden gümüş nanopartiküllerin sentezi, karekterizasyonu ve antimikrobial etkinliğinin incelenmesi. Journal of the Institute of Science and Technology, 9(3), 1467-1475.
  • Veisi, H., Azizi, S., & Mohammadi, P., (2018). Green synthesis of the silver nanoparticles mediated by Thymbra spicata extract and its application as a heterogeneous and recyclable nanocatalyst for catalytic reduction of a variety of dyes in water. Journal of Cleaner Production, 170, 1536-1543.

Camellia sinensis L. (Beyaz Çay Yaprağı) ile Gümüş Nanopartiküllerin Yeşil Sentezi ve Antibiyofilm Aktivitesi

Year 2024, Volume: 27 Issue: 2, 285 - 292, 01.04.2024

İlke Karakaş Nurcihan Hacıoğlu Bahri Emirhan Özdemir

https://doi.org/10.18016/ksutarimdoga.vi.1297130

Abstract

Green Synthesis and Antibiofilm Activity of Silver Nanoparticles by Camellia sinensis L. (White Tea Leaf)
Biosynthesis of nanoparticles refers to the production or synthesis of nanoparticles using organisms, such as bacteria, fungi, plants or their byproducts. This approach offers several advantages over conventional chemical methods, including eco-friendliness, cost-effectiveness, and potential for large-scale production. The silver nanoparticles (AgNPs) synthesized using aqueous Camellia sinensis L. (white tea leaf) extracts as reducing and stabilizing agent were reported and evaluated for antibiofilm activity against test microorganisms in the study. The synthesized AgNPs were observed and characterized using Uv-Vis spectroscopic analysis, scanning electron and transmission electron microscopy energy-dispersive spectra and Fourier transform infrared spectroscopy. The synthesized AgNP were also screened for antibiofilm activity against Acinetobacter baumanii ATCC 19606 NRRLB 3704, Pseudomonas aeruginosa ATCC 27853 (Gram –ve), and Bacillus subtilis ATCC 6633, Staphylococcus haemolyticus ATCC 43252 (Gram +ve), and Candida albicans ATCC 10231. It was observed that AgNPs may be a good alternative therapeutic approach in future. Our results show that the synthesized AgNPs has the potential to be used for antibiofilm materials and different biomedical applications.

Camellia sinensis L. (Beyaz Çay Yaprağı) ile Gümüş Nanopartiküllerin Yeşil Sentezi ve Antibiyofilm Aktivitesi
Nanopartiküllerin biyosentezi bakteriler, mantarlar, bitkiler veya yan ürünleri gibi organizmaları kullanarak nanopartiküllerin üretimini veay sentezini ifade eder. Bu yaklaşım, çevre dostu, maliyet etkinliği ve büyük ölçekli üretim potansiyeli dahil olmak üzere geleneksel kimyasal yöntemlere göre çeşitli avantajlar sunar. Indirgeyici ve stabilize edici ajan olarak Camellia sinensis L. (beyaz çay yaprağı) ekstraktları kullanılarak sentezlenen gümüş nanopartiküller (AgNP’ler) rapor edilmiş ve çalışmada test mikroorganizmalarına karşı antibiyofilm aktivitesi açısından değerlendirilmiştir. Sentezlenen AgNP’ler Uv-Vis spektroskopik analizi, taramalı electron mikroskobu, enerji dağıtıcı spektrumları ve Fourier dönüşümü kızılötesi spektroskopisi kullanılarak gözlemlendi ve karakterize edildi. Sentezlenen AgNP’nin Acinetobacter baumanii ATCC 19606 NRRLB 3704, Pseudomonas aeruginosa ATCC 27853 (Gram –), Bacillus subtilis ATCC 6633, Staphylococcus haemolyticus ATCC 43252 (Gram +), ve Candida albicans ATCC 10231’a karşı antibiyofilm aktivitesi de taranmıştır. AgNP’lerin gelecekte iyi bir alternative tedavi yaklaşımı olabileceği gözlenmiştir. Sonuçlarımız, sentezlenen AgNP'lerin antibiyofilm materyalleri ve farklı biyomedikal uygulamalar için kullanılma potansiyeline sahip olduğunu göstermektedir.Nanopartiküllerin biyosentezi bakteriler, mantarlar, bitkiler veya yan ürünleri gibi organizmaları kullanarak nanopartiküllerin üretimini veya sentezini ifade eder. Bu yaklaşım, çevre dostu, maliyet etkinliği ve büyük ölçekli üretim potansiyeli dahil olmak üzere geleneksel kimyasal yöntemlere göre çeşitli avantajlar sunar. İndirgeyici ve stabilize edici ajan olarak Camellia sinensis L. (beyaz çay yaprağı) ekstraktları kullanılarak sentezlenen gümüş nanopartiküller (AgNP’ler) rapor edilmiş ve çalışmada test mikroorganizmalarına (Acinetobacter baumanii ATCC 19606 NRRLB 3704, Pseudomonas aeruginosa ATCC 27853 (Gram –), Bacillus subtilis ATCC 6633, Staphylococcus haemolyticus ATCC 43252 (Gram +), ve Candida albicans ATCC 10231) karşı antibiyofilm aktivitesi açısından değerlendirilmiştir. Sentezlenen AgNP’ler Uv-Vis spektroskopik analizi, taramalı elektron mikroskobu, enerji dağıtıcı spektrumları ve Fourier dönüşümü kızılötesi spektroskopisi kullanılarak gözlemlendi ve karakterize edildi. Sentezlenen AgNP’nin test mikroorganizmalarına karşı antibiyofilm aktivitesi de taranmıştır. Sonuçlarımız, sentezlenen AgNP'lerin antibiyofilm materyalleri ve farklı biyomedikal uygulamalar için kullanılma potansiyeline sahip olduğunu göstermektedir.

Keywords

Camellia sinensis L. Gümüş Nanopartikül Antibiyofilm Aktivite

Project Number

2209-A

References

  • Atalar, M.N., Baran, A., Baran, M.F., Keskin, C., Aktepe, N., Yavuz, Ö., & İrtegun-Kandemir, S. (2022). Economic fast synthesis of olive leaf extract and silver nanoparticles and biomedical applications. Particulate Science and Technology, 40(5), 589-597.
  • Azizi, S., Shahri, M.M., Rahman, H.S., Rahim, R.A., Rasedee, A., & Mohamad, R., (2017). Green synthesis palladium nanoparticles mediated by white tea (Camellia sinensis) extract with antioxidants, antibacterial and antiproliferative activities towards the human leukaemia (MOLT-4) cell line. International Journal of Nanomedicine, 12, 8841-8853.
  • Baran, M.F. (2019). Synthesis and antimicrobial applications of silver nanoparticles from Artemisia absinthium plant. Biological and Chemical Research, 6, 96-103.
  • Barry, A.L., Craig, W.A., Nadler, H., Barth Reller, L., Sanders, C.C,, & Swenson, J.M., (1999). Methods for determining bactericidal activity of antimicrobial agents; approved guideline (4. edition), 1999, USA, Clinical and Laboratory Standards Institute.
  • Bayğu, G., (2020). Cimin üzümü yaprağı kullanılarak yeşil sentez yöntemiyle elde edilen gümüş nanopartikülünün genotoksik etkisinin kanat benek testi ile belirlenmesi. Master thesis, Erzincan Binali Yıldırım University.
  • Chandran, S.P., Chaudhary, M., Pasricha, R., Ahmad, A., & Sastry, M., (2006). Synthesis of gold nanotriangles and silver nanoparticles using Aloevera plant extract. Biotechnology progress, 22, 577-583.
  • El-Taher, E.M., (2011). Kombucha: A new microbial phenomenon and industrial benefits. African Journal of Biological Sciences, 7(2), 41-60.
  • Gan, P.P., & Li, S.F.Y., (2012). Potential of plant as a biological factory to synthesize gold and silver nanoparticles and their applications. Reviews in Environmental Science and Bio/Technology, 11(2), 169-206.
  • Ghaffari-Moghaddam, M., & Hadi-Dabanlou, R., (2014). Plant mediated green synthesis and antibacterial activity of silver nanoparticles using Crataegus douglasii fruit extract. Journal of Industrial and Engineering Chemistry, 20(2), 739-744.
  • Göl, F., Aygün, A., Seyrankaya, A., Gür, T., Yenikaya, C., & Şen, F., (2020). Green synthesis and characterization of Camellia sinensis mediated silver nanoparticles for antibacterial ceramic applications. Materials Chemistry and Physics, 250, 123-137.
  • Göse, M., & Hacıoğlu-Doğru, N., (2021). Bioactive compounds, antimicrobial and antibiofilm activity of two Verbascum species. KSÜ Tarım ve Doğa Dergisi 24(3), 479-487.
  • Hodoroaba, V., (2020). Energy-dispersive X-ray spectroscopy (EDS) Characterization of Nanoparticles, 397-417.
  • Ilgaz, A.Ş., Kalcıoğlu, Z., & İslamoğlu, E., (2006). Türk beyaz çayı üretim yönetiminin optimizasyonu ve Türk beyaz çayının kalite parametrelerinin belirlenmesi. Çaykur Çay İşletmeleri Genel Müdürlüğü Atatürk Çay ve Bahçe Kültürleri Araştırma Enstitüsü Teknoloji Kısım Müdürlüğü, 1-37.
  • İpek, İ., Baran, M.F., Baran, A., Hatipoğlu, A., Keskin, C., Yildiztekin, M., Küçükaydin, S., Becerekli, H., Kurt, K., Eftekhari, A., Huseynova, I., Khalilov, R., & Cho, W.C. (2023). Green synthesis and evaluation of antipathogenic, antioxidant, and anticholinesterase activities of gold nanoparticles (Au NPs) from Allium cepa L. peel aqueous extract. Biomass Conversion and Biorefnery, DOI: 10.1007/s13399-023-04362-y
  • Kouvaris, P., Delimitis, A., Zaspalis, V., Papadopoulos, D., Tsipas, S.A., & Michailidis, N., (2012). Green synthesis and characterization of silver nanoparticles produced using Arbutus unedo leaf extract. Materials Letters, 76, 18-20.
  • Kumar, S.P., Darshit, P., Ankita, P., Palak, D., Ram, P., Pradip, P., & Kaliaperumal, S., (2011). Biogenic synthesis of silver nanoparticles using Nicotiana tobaccum leaf extract and study of their antibacterial effect. African Journal of Biotechnology, 10(41), 8122-8130.
  • Merritt, J.H., Kadouri, D.E., & O’Toole, G.A., (2005). Growing and analyzing static biofilms. Current Protocol Microbiology,1(1B), 853.
  • Mohanta, B., Chakraborty, A., Selvaraj, S., & Roy, A., (2020). Bactericidal effect of gentamicin conjugated gold nanoparticles. Micro & Nano Letters, 15(10), 640-716.
  • Qi, P., Zhang, D., Zeng, Y., & Wan, Y., (2016). Biosynthesis of CdS nanoparticles: A fluorescent sensor for sulfate-reducing bacteria detection. Talanta, 147, 142-146.
  • Pallela, P.N.V.K., Ummey, S., Ruddaraju, L.K., Pammi, S.V.N., & Yoon, S.G., (2018). Ultra Small, mono dispersed green synthesized silver nanoparticles using aqueous extract of Sida cordifolia plant and investigation of antibacterial activity. Microbial Pathogenesis, 124, 63-69.
  • Salah, N., Miller, N.J., Paganga, G., Tijburg, L., Bolwell, G.P., & Rice-Evans, C., (1995). Polyphenolic flavanols as scavengers of aqueous phase radicals and as chain- breaking antioxidants. Archives of Biochemistry and Biophysics, 22, 339-346.
  • Składanowski, M., Wypij, M., Laskowski, D., Golińska, P., Dahm, H., & Rai, M., (2017). Silver and gold nanoparticles synthesized from Streptomyces sp. isolated from acid forest soil With special reference to its antibacterial activity against pathogens. Journal of Cluster Science, 28(1), 59-79.
  • Tamuly, C., Hazarika, M., Borah, S.C., Das, M.R., & Boruah, M.P., (2013). In situ biosynthesis of Ag, Au and bimetallic nanoparticles using Piper pedicellatum C.DC: green chemistry approach. Colloids and surfaces B: Biointerfaces, 102, 627-634.
  • Umaz, A. , Koç, A. , Baran, M.F. , Keskin, C. & Atalar, M.N. (2019). Hypericum Triquetrifolium Turra bitkisinden gümüş nanopartiküllerin sentezi, karekterizasyonu ve antimikrobial etkinliğinin incelenmesi. Journal of the Institute of Science and Technology, 9(3), 1467-1475.
  • Veisi, H., Azizi, S., & Mohammadi, P., (2018). Green synthesis of the silver nanoparticles mediated by Thymbra spicata extract and its application as a heterogeneous and recyclable nanocatalyst for catalytic reduction of a variety of dyes in water. Journal of Cleaner Production, 170, 1536-1543.
There are 25 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section RESEARCH ARTICLE
Authors

İlke Karakaş 0000-0001-6596-0879

Nurcihan Hacıoğlu 0000-0002-5812-9398

Bahri Emirhan Özdemir 0000-0002-5870-1171

Project Number 2209-A
Early Pub Date January 21, 2024
Publication Date April 1, 2024
Submission Date May 15, 2023
Acceptance Date October 12, 2023
Published in Issue Year 2024Volume: 27 Issue: 2

Cite

APA Karakaş, İ., Hacıoğlu, N., & Özdemir, B. E. (2024). Green Synthesis and Antibiofilm Activity of Silver Nanoparticles by Camellia sinensis L. (White Tea Leaf). Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(2), 285-292. https://doi.org/10.18016/ksutarimdoga.vi.1297130
 Download Cover Image


International Peer Reviewed Journal
Free submission and publication
Published 6 times a year



88x31.png


KSU Journal of Agriculture and Nature

e-ISSN: 2619-9149