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Investigation of the Genotoxic Effect of Fluoxetine Hydrochloride in Drosophila melanogaster

Year 2024, Volume: 27 Issue: 2, 316 - 324, 01.04.2024

Selda Öz , Zeynep Nur Sarıkaya , Özüm Larçın , Rabia Sarıkaya

https://doi.org/10.18016/ksutarimdoga.vi.1310729

Abstract

This study aimed to determine the potential genotoxic effect of fluoxetine hydrochloride (FLX-HCl), an antidepressant commonly used for treating depression, using Somatic Mutation and Recombination Test (SMART). Third-¬instar Drosophila melanogaster larvae transheterozygous for the mutations multiple wing hair (mwh) and flare (flr3) were chronically fed in a medium containing different concentrations of FLX-HCl (0.1, 0.5, 1, and 2 mg/mL) in the experimental group. Distilled water, 0.1 mM ethyl methane sulfonate (EMS), and 2% dimethyl sulfoxide (DMSO) were used in negative, positive, and solvent control groups, respectively. The survival percentages were calculated by determining the number of individuals surviving when the larvae completed their development in the experimental and control groups. In all application groups, the wings of 40 individuals with both normal and serrate wing phenotypes were examined under a microscope, and genetic changes were evaluated by counting the mutant clones in the wings. The data obtained show that 1 and 2 mg/mL concentrations of FLX-HCl caused toxic effects in D. melanogaster individuals. Additionally, FLX-HCl showed a negative genotoxic effect at 0.1 mg/mL concentration, insignificant at 0.5 mg/mL concentration, and positive at 1 and 2 mg/mL concentrations in terms of total mutation evaluation and clone induction frequency in D. melanogaster individuals.

Keywords

Drosophila melanogaster Fluoxetine hydrochloride Genotoxicity Survival SMART

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Fluoksetin Hidroklorür’ün Genotoksik Etkisinin Drosophila melanogaster’de Araştırılması

Year 2024, Volume: 27 Issue: 2, 316 - 324, 01.04.2024

Selda Öz , Zeynep Nur Sarıkaya , Özüm Larçın , Rabia Sarıkaya

https://doi.org/10.18016/ksutarimdoga.vi.1310729

Abstract

Bu çalışmada depresyon tedavisinde yaygın olarak kullanılan bir antidepresan olan fluoksetin hidroklorürün (FLX-HCl) potansiyel genotoksik etkisinin Somatik Mutasyon ve Rekombinasyon Testi (SMART) kullanılarak belirlenmesi amaçlanmıştır. Deney grubunda çoklu kanat kılı (mwh) ve flare (flr3) mutasyonları için transheterozigot olan üçüncü dönem Drosophila melanogaster larvaları, farklı konsantrasyonlarda FLX-HCl (0.1, 0.5, 1 ve 2 mg/mL) içeren ortamda kronik olarak beslenmiştir. Distile su, 0.1 mM etil metan sülfonat (EMS) ve % 2 dimetil sülfoksit (DMSO) sırasıyla negatif, pozitif ve çözücü kontrol gruplarında kullanılmıştır. Deney ve kontrol gruplarında larvalar gelişimini tamamladığında hayatta kalan birey sayıları belirlenerek yaşama yüzdeleri hesaplanmıştır. Tüm uygulama gruplarında hem normal hem de serrat kanat fenotipine sahip 40 bireyin kanatları mikroskop altında incelenmiş ve kanatlardaki mutant klonlar sayılarak genetik değişimler değerlendirilmiştir. Elde edilen veriler, FLX-HCl'nin 1 ve 2 mg/mL konsantrasyonlarının D. melanogaster bireyleri üzerinde toksik etkiye neden olduğunu göstermiştir. Ayrıca FLX-HCl, D. melanogaster bireylerinde toplam mutasyon değerlendirmesi ve klon induksiyon frekansı bakımından 0.1 mg/mL konsantrasyonunda negatif, 0.5 mg/mL konsantrasyonunda önemsiz, 1 ve 2 mg/mL konsantrasyonlarında pozitif genotoksik etki göstermiştir.

Keywords

Drosophila melanogaster Fluoksetin hidroklorür Genotoksisite Hayatta Kalış SMART

References

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Details

Primary Language English
Subjects Zoology (Other)
Journal Section RESEARCH ARTICLE
Authors

Selda Öz 0000-0003-1883-3441

Zeynep Nur Sarıkaya 0009-0003-9343-8887

Özüm Larçın 0009-0000-3232-9140

Rabia Sarıkaya 0000-0001-9247-8973

Early Pub Date January 21, 2024
Publication Date April 1, 2024
Submission Date June 7, 2023
Acceptance Date September 27, 2023
Published in Issue Year 2024Volume: 27 Issue: 2

Cite

APA Öz, S., Sarıkaya, Z. N., Larçın, Ö., Sarıkaya, R. (2024). Investigation of the Genotoxic Effect of Fluoxetine Hydrochloride in Drosophila melanogaster. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(2), 316-324. https://doi.org/10.18016/ksutarimdoga.vi.1310729
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