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Biological activities of Elaeagnus umbellata methanol extract

Year 2024, Volume: 27 Issue: 6, 1262 - 1268, 07.11.2024

Ülkü Zeynep Üreyen Esertaş , Merve Cora

https://doi.org/10.18016/ksutarimdoga.vi.1434448

Abstract

Elaeagnus umbellata, known as Autumn olive and growing widely in Asia and Southern Europe, is a shrub tree used in the traditional treatment of many diseases, including cancer. In this study, it was aimed to investigate the several biological effects of methanol extract of E. umbellate. The activity of the extract on 11 microorganisms was determined by the agar well diffusion technique. The anti-quorum sensing activity of the extract was tested using Chromobacterium violaceum ATCC 12472. The anti-biofilm and anti-swarming activities were tested using Pseudomonas aeruginosa PAO1. Cytotoxic effect of the extract against pancreatic tumoral cell line (AR42J), breast cancer cell line (MDA-MB-231), lung adenocarcinoma cell line (A549), and normal epithelial cell line (Vero), and antiviral effect of the extract against herpes simplex virus type 1 was analyzed using the MTT method. It was determined that the extract was moderately effective against 6/11 microorganisms and showed anti-quorum sensing activity. While the extract did not have a cytotoxic effect on cancer cell lines, it was found to have a cytotoxic effect on Vero cells at concentrations of 100 µg/mL and above. However, no anti-biofilm, anti-swarming, and antiviral activity of the extract was observed. The study shows that E. umbellata fruit has limited biological activity.

Keywords

Antimicrobial Elaeagnus umbellata Anti-quorum sensing Anticancer

References

  • Ahmad, S.D., Sabir, M.S., Juma, M., Asad, H.S. (2005). Morphological and biochemical variations in Elaeagnus umbellata Thunb. from mountains of Pakistan. Acta Botanica Croatica, 64(1), 121–128.
  • Ak, B. E., Fidancı, A., & Hatipoglu, I. H. (2022). Schefflera arbicola (Hayata) Merr.Bitkisinin Çelikle Çoğaltılmasında Farklı Yetiştirme Ortamları ile Oksinlerin ve Konsantrasyonların Etkisi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(5), 1023-1027.
  • Aziz, S., Rehman, H., Andleeb, S. (2015). Biological screening of Elaeagnus umbellata Thunb. Pakistan Journal of Pharmaceutical Sciences, 28(1),65–70.
  • Bhat, M. A.,Mishra, A.K., Kamal, M.A., Rahman, S., & Jan, A.T. (2023). Elaeagnus umbellata: A miraculous shrub with potent health-promoting benefits from Northwest Himalaya. Saudi Journal of Biological Sciences, 30(6), 103662.
  • Cora, M., Buruk, C.K., Unsal, S., Kaklikkaya, N., Kolayli, S. (2023). Chemical Analysis and in Vitro Antiviral Effects of Northeast Türkiye Propolis Samples against HSV-1. Chemistry & Biodiversity,20(8), e202300669.
  • Ekşi, S., Esertaş, Ü.Z.Ü., Kilic, A.O., Ejder, N., Uzunok, B. (2020). Determination of the antimicrobial and anti-biofilm effects and ‘quorum sensing’ inhibition potentials of Castanea sativa mill. extracts. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(1),66–78.
  • Erdonmez, D., Kenar, N. & Turkmen, K.E. (2016). Evaluation of anti-quorum sensing activity of Elaeagnus angustifolia L. extracts from Central Anatolia. Journal of Biotechnology, 231-34.
  • Gür, D. (2016). Antibiyotik Duyarlılık Testleri, EUCAST: Uygulama, Yorum ve Uzman Kurallar. Türk Mikrobiyoloji Cemiyeti Dergisi ,46.
  • Kang, H.R., Jung, J.K., Park, S.H., Lee, J.Y., Yang, S.A. (2020). Antioxidant, anti-inflammatory, and anti-bacterial effects of extracts from Elaeagnus umbellate leaves obtained using different extract conditions. Korean Journal Food Preservation, 27(3),374-384.
  • Nauman, K., & Arshad, M. (2011). Screening of aqueous methanol plant extracts for their antibacterial activity. In Science And Technology Against Microbial Pathogens: Research, Development and Evaluation (pp. 123-127).
  • Nazir, N., Zahoor, M., Nisar, M., Khan, I., Karim, N., Abdel-Halim, H., Ali, A. (2018). Phytochemical analysis and antidiabetic potential of Elaeagnus umbellata (Thunb.) in streptozotocin-induced diabetic rats: Pharmacological and computational approach. BMC Complementary and alternative Medicine, 18, 1-16.
  • Nazir, N., Zahoor, M., Nisar, M., Khan, I., Ullah, R., Alotaibi, A. (2021). Antioxidants Isolated from Elaeagnus umbellata (Thunb.) Protect against Bacterial Infections and Diabetes in Streptozotocin-Induced Diabetic Rat Model. Molecules, 26(15), 4464.
  • Nazir, N., Zahoor, M., Nisar, M., Karim, N., Latif, A., Ahmad, S., Uddin, Z. (2020). Evaluation of neuroprotective and anti-amnesic effects of Elaeagnus umbellata Thunb. On scopolamine-induced memory impairment in mice. BMC Complementary Medicine and Therapies, 20,1-17.
  • Olas, B., Skalski, B. (2022). Preparations from Various Organs of Sea Buckthorn (Elaeagnus rhamnoides (L.) A. Nelson) as Important Regulators of Hemostasis and Their Role in the Treatment and Prevention of Cardiovascular Diseases. Nutrients, 14(5), 991.
  • Patel, S. (2015). Plant genus Elaeagnus: underutilized lycopene and linoleic acid reserve with permaculture potential. Fruits, 70(4),191–199.
  • Parekh, J., Jadeja, D., & Chanda, S. (2005). Efficacy of aqueous and methanol extracts of some medicinal plants for potential antibacterial activity. Turkish Journal of Biology, 29(4), 203-210.
  • Rashid, M.H., Kornberg, A. (2000). Inorganic polyphosphate is needed for swimming, swarming, and twitching motilities of Pseudomonas aeruginosa. Proceedins of the National Academy of Science, 97(9),4885-90.
  • Sabir, M.S., Ahmad, D.S., Imtiaz, H., Tahir, K.M. (2007). Antibacterial activity of Elaeagnus umbellata (Thunb.) a medicinal plant from Pakistan. Saudi Medical Journal, 28 (2),259.
  • Saraç, H., & Özpınar, H. (2024). The Effect of Nettle (Urtica dioica L.), Carob (Ceratonia siliqua L.), and Chaste (Vitex agnus-castus L.) Plants on Fertility in Caenorhabditis elegans. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(4), 758-766.
  • Temel, M., Tinmaz, A. B., Öztürk, M., Gündüz, O. (2018). Dünyada ve Türkiye’de Tıbbi -Aromatik Bitkilerin Üretimi ve Ticareti. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 21,198-214
  • Truchado, P., Gil-Izqierdo, A., Tomas-Barberan, F., Allenda, A. (2009). Inhibition by chestnut honey of N-Acyl-L-homoserinelactones and biofilm formation in Erwinia carotovora, Yersinia enterocolitica and Aeromonas hydrophila. Journal of Agricultural and Food Chemistry, 57(23),11186-11193.
  • Uddin, G. & Rauf, A. (2012). Phytochemical screening and biological activity of the aerial parts of Elaeagnus umbellate. Scientific Research and Essays 7(43), 3690–3694.
  • Üreyen Esertaş, Ü. Z., Kara, Y., Kiliç, A. O., & Kolayli, S. (2022). A comparative study of antimicrobial, anti-quorum sensing, anti-biofilm, anti-swarming, and antioxidant activities in flower extracts of pecan (Carya illinoinensis) and chestnut (Castanea sativa). Archives of Microbiology, 204(9), 589.
  • Wang, S.Y., Bowman, L., Ding, M. (2007). Variations in Free Radical Scavenging Capacity and Antiproliferative Activity Among Different Genotypes of Autumn Olive (Elaeagnus umbellata). Planta Medica, 73(5), 468-477.
  • Woods, G.L., Brown-Elliott, B.A., Desmond, E.P., Hall, G.S., Heifets, L. (2003). Susceptibility testing of mycobacteria, nocardiae, and other aerobic actinomycetes; Approved Standard. NCCLS document M24-A, 23 (18).
  • Wu, M.C., Hu, H.T., Yang, L. (2011). Proteomic analysis of up-accumulated proteins associated with fruit quality during autumn olive (Elaeagnus umbellata) fruit ripening. Journal of Agricultural Food Chemistry,59(2),577–83.
  • Zulfiqar, H., Amjad, M.S., Mehmood, A., Mustafa, G.; Binish, Z.; Khan, S.; Arshad, H.; Proćków, J.; Pérez de la Lastra, J.M. (2022). Antibacterial, Antioxidant, and Phytotoxic Potential of Phytosynthesized Silver Nanoparticles Using Elaeagnus umbellata Fruit Extract. Molecules, 27(18),5847.

Elaeagnus umbellata metanol ekstraktının biyolojik aktivitesi

Year 2024, Volume: 27 Issue: 6, 1262 - 1268, 07.11.2024

Ülkü Zeynep Üreyen Esertaş , Merve Cora

https://doi.org/10.18016/ksutarimdoga.vi.1434448

Abstract

Asya ve Güney Avrupa’da yaygın olarak yetişen ve Sonbahar zeytini olarak bilinen Elaeagnus umbellata, kanser dahil olmak üzere birçok hastalığın geleneksel tedavisinde kullanılan bir çalı ağacıdır. Bu çalışmanın amacı E. umbellate ekstraktının çeşitli biyolojik etkilerinin belirlenmesidir. Ekstraktın 11 mikroorganizma üzerindeki aktivitesi agar kuyucuk difüzyon tekniği ile belirlendi. Ekstraktın anti-quorum sensing aktivitesi Chromobacterium violaceum ATCC 12472, anti-biyofilm ve anti-swarming aktiviteleri ise Pseudomonas aeruginosa PAO1 kullanılarak test edildi. Ekstraktın pankreatik tümöral hücre hattı (AR42J), meme kanseri hücre hattı (MDA-MB-231), akciğer adenokarsinoma hücre hattı (A549) ve normal epitel hücre hattı (Vero)’nasitotoksik etkisi ve herpes simpleks virüs tip-1’e karşı antiviral etkisi MTT yöntemi ile araştırıldı. Ekstraktın 6/11 mikroorganizmaya karşı orta düzeyde etkili olduğu ve anti-quorum sensing aktivite gösterdiği belirlendi. Ekstraktın kanser hücre hatlarında sitotoksk etkisi görülmezken, Vero hücrelerine 100 µg/mL ve üzeri konsantrasyonlarda sitotoksik etkisi olduğu tespit edildi. Bununla birlikte ekstraktın anti-biyofilm, anti-swarming ve antiviral aktivitesi görülmedi. Yapılan çalışma E. umbellata meyvesinin sınırlı biyolojik aktivitesinin olduğunu göstermektedir.

Keywords

Elaeagnus umbellata Antimikrobiyal Anti-quorum sensing Antikanser

References

  • Ahmad, S.D., Sabir, M.S., Juma, M., Asad, H.S. (2005). Morphological and biochemical variations in Elaeagnus umbellata Thunb. from mountains of Pakistan. Acta Botanica Croatica, 64(1), 121–128.
  • Ak, B. E., Fidancı, A., & Hatipoglu, I. H. (2022). Schefflera arbicola (Hayata) Merr.Bitkisinin Çelikle Çoğaltılmasında Farklı Yetiştirme Ortamları ile Oksinlerin ve Konsantrasyonların Etkisi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(5), 1023-1027.
  • Aziz, S., Rehman, H., Andleeb, S. (2015). Biological screening of Elaeagnus umbellata Thunb. Pakistan Journal of Pharmaceutical Sciences, 28(1),65–70.
  • Bhat, M. A.,Mishra, A.K., Kamal, M.A., Rahman, S., & Jan, A.T. (2023). Elaeagnus umbellata: A miraculous shrub with potent health-promoting benefits from Northwest Himalaya. Saudi Journal of Biological Sciences, 30(6), 103662.
  • Cora, M., Buruk, C.K., Unsal, S., Kaklikkaya, N., Kolayli, S. (2023). Chemical Analysis and in Vitro Antiviral Effects of Northeast Türkiye Propolis Samples against HSV-1. Chemistry & Biodiversity,20(8), e202300669.
  • Ekşi, S., Esertaş, Ü.Z.Ü., Kilic, A.O., Ejder, N., Uzunok, B. (2020). Determination of the antimicrobial and anti-biofilm effects and ‘quorum sensing’ inhibition potentials of Castanea sativa mill. extracts. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(1),66–78.
  • Erdonmez, D., Kenar, N. & Turkmen, K.E. (2016). Evaluation of anti-quorum sensing activity of Elaeagnus angustifolia L. extracts from Central Anatolia. Journal of Biotechnology, 231-34.
  • Gür, D. (2016). Antibiyotik Duyarlılık Testleri, EUCAST: Uygulama, Yorum ve Uzman Kurallar. Türk Mikrobiyoloji Cemiyeti Dergisi ,46.
  • Kang, H.R., Jung, J.K., Park, S.H., Lee, J.Y., Yang, S.A. (2020). Antioxidant, anti-inflammatory, and anti-bacterial effects of extracts from Elaeagnus umbellate leaves obtained using different extract conditions. Korean Journal Food Preservation, 27(3),374-384.
  • Nauman, K., & Arshad, M. (2011). Screening of aqueous methanol plant extracts for their antibacterial activity. In Science And Technology Against Microbial Pathogens: Research, Development and Evaluation (pp. 123-127).
  • Nazir, N., Zahoor, M., Nisar, M., Khan, I., Karim, N., Abdel-Halim, H., Ali, A. (2018). Phytochemical analysis and antidiabetic potential of Elaeagnus umbellata (Thunb.) in streptozotocin-induced diabetic rats: Pharmacological and computational approach. BMC Complementary and alternative Medicine, 18, 1-16.
  • Nazir, N., Zahoor, M., Nisar, M., Khan, I., Ullah, R., Alotaibi, A. (2021). Antioxidants Isolated from Elaeagnus umbellata (Thunb.) Protect against Bacterial Infections and Diabetes in Streptozotocin-Induced Diabetic Rat Model. Molecules, 26(15), 4464.
  • Nazir, N., Zahoor, M., Nisar, M., Karim, N., Latif, A., Ahmad, S., Uddin, Z. (2020). Evaluation of neuroprotective and anti-amnesic effects of Elaeagnus umbellata Thunb. On scopolamine-induced memory impairment in mice. BMC Complementary Medicine and Therapies, 20,1-17.
  • Olas, B., Skalski, B. (2022). Preparations from Various Organs of Sea Buckthorn (Elaeagnus rhamnoides (L.) A. Nelson) as Important Regulators of Hemostasis and Their Role in the Treatment and Prevention of Cardiovascular Diseases. Nutrients, 14(5), 991.
  • Patel, S. (2015). Plant genus Elaeagnus: underutilized lycopene and linoleic acid reserve with permaculture potential. Fruits, 70(4),191–199.
  • Parekh, J., Jadeja, D., & Chanda, S. (2005). Efficacy of aqueous and methanol extracts of some medicinal plants for potential antibacterial activity. Turkish Journal of Biology, 29(4), 203-210.
  • Rashid, M.H., Kornberg, A. (2000). Inorganic polyphosphate is needed for swimming, swarming, and twitching motilities of Pseudomonas aeruginosa. Proceedins of the National Academy of Science, 97(9),4885-90.
  • Sabir, M.S., Ahmad, D.S., Imtiaz, H., Tahir, K.M. (2007). Antibacterial activity of Elaeagnus umbellata (Thunb.) a medicinal plant from Pakistan. Saudi Medical Journal, 28 (2),259.
  • Saraç, H., & Özpınar, H. (2024). The Effect of Nettle (Urtica dioica L.), Carob (Ceratonia siliqua L.), and Chaste (Vitex agnus-castus L.) Plants on Fertility in Caenorhabditis elegans. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(4), 758-766.
  • Temel, M., Tinmaz, A. B., Öztürk, M., Gündüz, O. (2018). Dünyada ve Türkiye’de Tıbbi -Aromatik Bitkilerin Üretimi ve Ticareti. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 21,198-214
  • Truchado, P., Gil-Izqierdo, A., Tomas-Barberan, F., Allenda, A. (2009). Inhibition by chestnut honey of N-Acyl-L-homoserinelactones and biofilm formation in Erwinia carotovora, Yersinia enterocolitica and Aeromonas hydrophila. Journal of Agricultural and Food Chemistry, 57(23),11186-11193.
  • Uddin, G. & Rauf, A. (2012). Phytochemical screening and biological activity of the aerial parts of Elaeagnus umbellate. Scientific Research and Essays 7(43), 3690–3694.
  • Üreyen Esertaş, Ü. Z., Kara, Y., Kiliç, A. O., & Kolayli, S. (2022). A comparative study of antimicrobial, anti-quorum sensing, anti-biofilm, anti-swarming, and antioxidant activities in flower extracts of pecan (Carya illinoinensis) and chestnut (Castanea sativa). Archives of Microbiology, 204(9), 589.
  • Wang, S.Y., Bowman, L., Ding, M. (2007). Variations in Free Radical Scavenging Capacity and Antiproliferative Activity Among Different Genotypes of Autumn Olive (Elaeagnus umbellata). Planta Medica, 73(5), 468-477.
  • Woods, G.L., Brown-Elliott, B.A., Desmond, E.P., Hall, G.S., Heifets, L. (2003). Susceptibility testing of mycobacteria, nocardiae, and other aerobic actinomycetes; Approved Standard. NCCLS document M24-A, 23 (18).
  • Wu, M.C., Hu, H.T., Yang, L. (2011). Proteomic analysis of up-accumulated proteins associated with fruit quality during autumn olive (Elaeagnus umbellata) fruit ripening. Journal of Agricultural Food Chemistry,59(2),577–83.
  • Zulfiqar, H., Amjad, M.S., Mehmood, A., Mustafa, G.; Binish, Z.; Khan, S.; Arshad, H.; Proćków, J.; Pérez de la Lastra, J.M. (2022). Antibacterial, Antioxidant, and Phytotoxic Potential of Phytosynthesized Silver Nanoparticles Using Elaeagnus umbellata Fruit Extract. Molecules, 27(18),5847.
There are 27 citations in total.

Details

Primary Language English
Subjects Microbiology (Other)
Journal Section RESEARCH ARTICLE
Authors

Ülkü Zeynep Üreyen Esertaş 0000-0001-9897-5313

Merve Cora 0000-0002-5956-9133

Early Pub Date August 15, 2024
Publication Date November 7, 2024
Submission Date February 9, 2024
Acceptance Date May 27, 2024
Published in Issue Year 2024Volume: 27 Issue: 6

Cite

APA Üreyen Esertaş, Ü. Z., & Cora, M. (2024). Biological activities of Elaeagnus umbellata methanol extract. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(6), 1262-1268. https://doi.org/10.18016/ksutarimdoga.vi.1434448
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