Determination of in vitro Antioxidant, Anticholinergic, and Antiepileptic Activities of some Medicinal and Aromatic Plant Extracts

Bayram Yurt; Rüya Sağlamtaş; Yeliz Demir; Ebubekir İzol; Halit Diril; Cüneyt Çağlayan

doi:10.18016/ksutarimdoga.vi.1472403

Araştırma Makalesi

Determination of in vitro Antioxidant, Anticholinergic, and Antiepileptic Activities of some Medicinal and Aromatic Plant Extracts

Yıl 2024, Cilt: 27 Sayı: Ek Sayı 1 (Suppl 1), 1 - 15

Bayram Yurt , Rüya Sağlamtaş , Yeliz Demir , Ebubekir İzol , Halit Diril , Cüneyt Çağlayan

https://doi.org/10.18016/ksutarimdoga.vi.1472403

Öz

Medicinal and aromatic plants such as Crocus cancellatus, Scilla Siberica subsp. armena, Juniperus oxycedrus subsp. oxycedrus and Anthriscus nemorosa have many different biological activities. While antioxidants are significant in preventing many diseases, inhibition of metabolic enzymes is also effective in preventing many diseases. In this study, antioxidant activities of water, ethanol, and dichloromethane extracts of four different medicinal and aromatic plant species were determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH•) and 2,20-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS•+) radical scavenging and Cu2+, Fe+3, and Fe3+-2,4,6-tris(2-pyridyl)-S-triazine (TPTZ) reducing assays. Enzyme inhibition studies were performed with metabolic enzymes acetylcholinesterase, butyrylcholinesterase, carbonic anhydrase I and II isoenzymes. The ethanol extract of A. nemorosa showed the highest activity in DPPH and ABTS assays (IC50: 17.36 µg mL-1, IC50: 7.02 µg mL-1). In the Fe3+ reducing assay, the dichloromethane extract of A. nemorosa showed the highest activity (1.96±0.060 µg mL-1). In the Cu2+ reducing assay, the dichloromethane extract of J. oxycedrus showed the highest activity (1.773±0.066 µg mL-1). In the Fe3+-TPTZ reducing assay, the ethanol extract of S. siberica showed the highest activity (1.256±0.011 µg mL-1). In the enzyme inhibition results, it was determined that all plants and all extracts inhibited the enzymes studied. As a result of this study, it was determined that these four medicinal and aromatic plants have high biological activities.

Anahtar Kelimeler

Crocus cancellatus, Scilla siberica, Juniperus oxycedrus, Anthriscus nemorosa, Antioxidant and enzyme inhibition

Kaynakça

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Bazı Tıbbi ve Aromatik Bitki Ekstraktlarının in vitro Antioksidan, Antikolinerjik ve Antiepileptik Aktivitelerinin Belirlenmesi

Yıl 2024, Cilt: 27 Sayı: Ek Sayı 1 (Suppl 1), 1 - 15

Bayram Yurt , Rüya Sağlamtaş , Yeliz Demir , Ebubekir İzol , Halit Diril , Cüneyt Çağlayan

https://doi.org/10.18016/ksutarimdoga.vi.1472403

Öz

Crocus cancellatus, Scilla siberica subsp. armena, Juniperus oxycedrus subsp. oxycedrus ve Anthriscus nemorosa gibi tıbbi ve aromatik bitkiler birçok farklı biyolojik aktiviteye sahiptir. Antioksidanlar birçok hastalığın önlenmesinde önemli rol oynarken, metabolik enzimlerin inhibisyonu da birçok hastalığın önlenmesinde etkilidir. Bu çalışmada, dört farklı tıbbi ve aromatik bitki türünün su, etanol ve diklorometan ekstraktlarının antioksidan aktiviteleri 1,1-difenil-2-pikrilhidrazil (DPPH•) ve 2,20-azino-bis-3-etilbenzthiazoline-6-sülfonik asit (ABTS•+) radikal giderme ve Cu2+, Fe3+ ve Fe3+-TPTZ indirgeme deneyleri ile belirlenmiştir. Enzim inhibisyon çalışmaları metabolik enzimler olan asetilkolinesteraz, bütirilkolinesteraz, karbonik anhidraz I ve II izoenzimleri ile gerçekleştirilmiştir. A. nemorosa'nın etanol ekstresi DPPH ve ABTS deneylerinde en yüksek aktiviteyi göstermiştir (IC50: 17.36 µg mL-1, IC50: 7.02 µg mL-1). Fe3+ indirgeme deneyinde, A. nemorosa'nın diklorometan ekstresi en yüksek aktiviteyi göstermiştir (1.96±0.060 µg mL-1). Cu2+ indirgeme deneyinde, J. oxycedrus'un diklorometan ekstresi en yüksek aktiviteyi göstermiştir (1.773±0.066 µg mL-1). Fe3+-TPTZ indirgeme deneyinde, S. siberica'nın etanol ekstraktı en yüksek aktiviteyi göstermiştir (1.256±0.011 µg mL-1). Enzim inhibisyon sonuçlarında, tüm bitkilerin çalışılan enzimleri inhibe ettiği belirlenmiştir. Bu çalışma sonucunda tıbbi ve aromatik bitkilerden olan bu dört bitkinin yüksek biyolojik aktiviteye sahip olduğu belirlenmiştir.

Anahtar Kelimeler

Crocus cancellatus, Scilla siberica, Juniperus oxycedrus, Anthriscus nemorosa, Antioksidan ve enzim inhibisyonu

Etik Beyan

The study does not require ethics committee declaration.

Kaynakça

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Toplam 77 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	İngilizce
Konular	Bitki Biyokimyası
Bölüm	ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar	Bayram Yurt 0000-0001-5447-1586 Rüya Sağlamtaş 0000-0002-4400-2302 Yeliz Demir 0000-0003-3216-1098 Ebubekir İzol 0000-0003-0788-4999 Halit Diril 0000-0003-4409-8268 Cüneyt Çağlayan 0000-0001-5608-554X
Erken Görünüm Tarihi	15 Eylül 2024
Yayımlanma Tarihi
Gönderilme Tarihi	23 Nisan 2024
Kabul Tarihi	9 Ağustos 2024
Yayımlandığı Sayı	Yıl 2024Cilt: 27 Sayı: Ek Sayı 1 (Suppl 1)

Kaynak Göster

APA	Yurt, B., Sağlamtaş, R., Demir, Y., İzol, E., vd. (2024). Determination of in vitro Antioxidant, Anticholinergic, and Antiepileptic Activities of some Medicinal and Aromatic Plant Extracts. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(Ek Sayı 1 (Suppl 1), 1-15. https://doi.org/10.18016/ksutarimdoga.vi.1472403