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A Scanning Electron Microscope Study of Vegetative Parts of the Genus Vincetoxicum in Türkiye

Year 2025, Volume: 28 Issue: 2, 380 - 402, 27.03.2025

Seher Güven , Selin Meral Doğan

https://doi.org/10.18016/ksutarimdoga.vi.1589750

Abstract

In Türkiye, Vincetoxicum is represented by 11 taxa belonging to nine species, three of which (V. coskuncelebiana S.Makbul & S.Güven, V. fuscatum (Hornem.) subsp. boissieri (Kusn.) Browicz, and V. parviflorum Decne) are endemics. In this study, the micro-morphology of the stem, petiole, and lamina of all Turkish Vincetoxicum taxa was examined using a scanning electron microscope (SEM) for the first time. Micro-morphological observations were carried out on herbarium samples belonging to 21 populations from various habitats. A detailed micro-morphological description of the stem, petiole, and lamina, supported by SEM micrographs, was created for each examined taxon. It was determined that the micro-morphological characteristics such as the pattern of pubescence, the distribution and density of trichomes on the stem, petiole, and lamina, and the position of stomata on both sides of the leaf contributed to the distinction of the Turkish Vincetoxicum taxa. On the other hand, no difference was observed among the examined taxa in terms of the appearance of epicuticular wax, and shape and concavity/convexity properties of epidermal cells on stem, petiole, and lamina.

Keywords

Apocynaceae Leaf SEM Stem Vincetoxicum

Supporting Institution

This study was supported by Scientific and Technological Research Council of Turkey (TUBITAK-2209A) under the Grant Number 1919B012004568. The authors thank to TUBITAK for their supports.

Project Number

TUBITAK-2209A/ Proje no: 1919B012004568

Thanks

The authors would like to thank Prof. Dr. Serdar MAKBUL, who allowed the stem and leaf materials used in the study to be obtained from the samples of the examined species.

References

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Türkiye Vincetoxicum Cinsinin Vejetatif Kısımlarının Taramalı Elektron Mikroskobu İle İncelenmesi

Year 2025, Volume: 28 Issue: 2, 380 - 402, 27.03.2025

Seher Güven , Selin Meral Doğan

https://doi.org/10.18016/ksutarimdoga.vi.1589750

Abstract

Vincetoxicum, Türkiye’de üçü endemik (V. coskuncelebiana S.Makbul & S.Güven, V. fuscatum (Hornem.) subsp. boissieri (Kusn.) Browicz ve V. parviflorum Decne) olmak üzere dokuz türe ait 11 takson ile temsil edilmektedir. Bu çalışmada, tüm Türkiye Vincetoxicum taksonlarının gövde, petiyol ve lamina mikro-morfolojisi taramalı elektron mikroskobu (SEM) kullanılarak ilk kez taksonomik yönden değerlendirilmiştir. Mikro-morfolojik incelemeler, farklı habitatlardan toplanan 21 populasyona ait herbaryum örnekleri üzerinde gerçekleştirilmiştir. İncelenen her takson için SEM fotoğraflarıyla desteklenen ayrıntılı birer gövde, petiyol ve lamina mikro-morfolojik betimi oluşturulmuştur. Türkiye Vincetoxicum taksonlarının ayrımında; gövde, petiyol ve lamina yüzeylerindeki tüylenme çeşidi, tüy dağılımı ve yoğunluğu ile yaprağın her iki yüzeyindeki stoma durumu gibi mikromorfolojik karakterlerin önemli rol oynadığı belirlenmiştir. Diğer taraftan, incelenen taksonlar arasında gövde, yaprak sapı ve lamina üzerindeki epikütikular mumun görünümü, epidermis hücrelerinin şekli ve içbükeylik/dışbükeylik özellikleri bakımından bir fark görülmemiştir.

Keywords

Apocynaceae Gövde SEM Vincetoxicum Yaprak

Supporting Institution

This study was supported by Scientific and Technological Research Council of Turkey (TUBITAK-2209A) under the Grant Number 1919B012004568. The authors thank to TUBITAK for their supports.

Project Number

TUBITAK-2209A/ Proje no: 1919B012004568

Thanks

The authors would like to thank Prof. Dr. Serdar MAKBUL, who allowed the stem and leaf materials used in the study to be obtained from the samples of the examined species.

References

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  • Abeysinghe, P. D., & Scharaschkin, T. (2022). Comparative anatomy of two forms of Sri Lankan Calotropis gigantea (L.) R. Br. (Family Apocynaceae s.l.–Subfamily Asclepiadoideae) -Taxonomic implications. Ceylon Journal of Science, 51(3), 307–318. http://doi.org/10.4038/cjs.v51i3.8038
  • Aybeke, M. ( 2022). Bazı Orkide taksonlarında labellum mikromorfolojisi. KSÜ Tarım ve Doğa Derg, 25(1): 42– 48. https://doi.org/10.18016/ksutarimdoga.vi.882554
  • Barthlott, W., Neinhuis, C., Cutler, D., Ditsch, F., Meusel, I., Theisen, I., & Wilhelmi, H. (1998). Classification and terminology of plant epicuticular waxes. Botanical Journal of the Linnean Society, 126(3), 237–260. https://doi.org/10.1111/j.1095-8339.1998.tb02529.x
  • Bashir, K., Sohail, A., Ali, U., Ullah, A., Ul Haq, Z., Gul, B., Ullah, I., Sunera, & Asghar, M. (2020). Foliar micromorphology and its role in identification of the Apocynaceae taxa. Microscopy Research and Technique, 83, 755–766.
  • Behnke, H. D., & Barthlott, W. (1983). New evidence from the ultrastructural and micromorphological fields in angiosperm classification. Nordic Journal of Botany, 3, 43–66. https://doi.org/10.1111/j.1756-1051. 1983.tb01444.x
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  • Carpenter, K. J. (2005). Stomatal architecture and evolution in basal angiosperms. American Journal of Botany, 92, 1595–1615. https://doi.org/10.3732/ajb.92.10.1595
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  • Civeyrel, L., Thomas, A. L., Ferguson, K., & Chase, M. W. (1998). Critical reexamination of palynological characters used to delimit Asclepiadaceae in comparison to the molecular phylogeny obtained from plastid matK sequences. Molecular Phylogenetics and Evolution, 9(3), 517–527. https://doi.org/10.1006/mpev.1997.0510
  • Davis, P. H. (1965). Flora of Türkiye and the East Aegean Islands, Vol. 1. Edinburgh: Edinburgh University Press.
  • Dilcher, D. L. (1974). Approaches to the identification of angiosperm leaf remains. Botanical Review, 40(1), 1–160. https://doi.org/10.1007/BF02860067
  • El-Taher, A. M., Gendy, A. E. -N. G. E., Alkahtani, J., Elshamy, A. I., & Abd-ElGawad, A. M. (2020). Taxonomic implication of integrated chemical, morphological, and anatomical attributes of leaves of eight Apocynaceae taxa. Diversity, 12(9), 334. https://doi.org/10.3390/d12090334
  • Feng. Y., Qin. X., Huang. J., & Hong, W. (2012). Pollinarium morphology of 17 species and 1 subspecies of Cynanchum (Asclepiadaceae). Acta Botanica Boreali-Occidentalia Sinica, 32(9),1758–1762.
  • Freire, S. E., Arambarri, A. M., Bayón, N.D., Sancho, G., Urtubey, E., Monti, C., Novoa, M.C., & Colares, M. N. (2005). Epidermal characteristics of toxic plants for cattle from the Salado river basin (Buenos Aires, Argentina). Boletín de la Sociedad Argentina de Botánica, 40, 241–281.
  • Fishbein, M. (2001). Evolutionary innovation and diversification in the flowers of Asclepiadaceae. Annals of the Missouri Botanical Garden, 88, 603–623. http://dx.doi.org/10.2307/3298636
  • Gabr, D. G., Khafagi, A. A. F., Mohamed, A. H., & Mohamed, F. S. (2015). The significance of leaf morphological characters in the identification of some species of Apocynaceae and Asclepiadaceae. Journal of American Science, 11, 61–70.
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Details

Primary Language English
Subjects Plant Morphology and Anatomy
Journal Section RESEARCH ARTICLE
Authors

Seher Güven 0000-0001-8883-8489

Selin Meral Doğan 0009-0009-6051-5093

Project Number TUBITAK-2209A/ Proje no: 1919B012004568
Early Pub Date March 20, 2025
Publication Date March 27, 2025
Submission Date November 23, 2024
Acceptance Date January 28, 2025
Published in Issue Year 2025Volume: 28 Issue: 2

Cite

APA Güven, S., & Doğan, S. M. (2025). A Scanning Electron Microscope Study of Vegetative Parts of the Genus Vincetoxicum in Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(2), 380-402. https://doi.org/10.18016/ksutarimdoga.vi.1589750
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