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Chemical Composition and Antimicrobial Effect of Essential Oil of Anthemis pauciloba Boiss. var. pauciloba from Türkiye

Year 2025, Volume: 28 Issue: 3, 617 - 624

Damla Kırcı , Ayla Kaya , Betül Demirci , Süleyman Doğu

https://doi.org/10.18016/ksutarimdoga.vi.1402876

Abstract

Anthemis pauciloba Boiss. var. pauciloba is one of four recognized varieties in Türkiye, locally known as “bol papatya.” Its flowers are traditionally used as a cold infusion to treat asthma. The aim of the research was to determine the chemical composition of essential oil (EO) of A. pauciloba var. pauciloba aerial parts obtained by hydrodistillation using a Clevenger-type apparatus, examined by GC-FID, and GC-MS, simultaneously. The EO was evaluated for antibacterial and antifungal activities against microbial strains utilizing the broth-microdilution technique. α-Thujone (28.7%), α-pinene (26.7%), and β-thujone (9.0%) were found as the main constituents of EO. The antimicrobial activity (Minimum Inhibitory Concentration) against gram-negative, gram-positive, and yeast was observed by the essential oil. The essential oil demonstrated the highest antimicrobial activity against Candida krusei (MIC: 1.25 mg/mL). The antimicrobial activity of the essential oil from the aerial parts of A. pauciloba var. pauciloba was evaluated for the first time in this study.

Keywords

Anthemis pauciloba var. pauciloba Asteraceae Essential oil Antimicrobial activity

References

  • Babushok, V. I., Linstrom, P. J., & Zenkevich, I. G. (2011). Retention indices for frequently reported compounds of plant essential oils. Journal of Physical and Chemical Reference Data, 40(1), 1–47. https://doi.org/ 10.1063/1.3653552
  • Bassolé, I. H. N., & Juliani, H. R. (2012). Essential oils in combination and their antimicrobial properties. Molecules 17(4), 3989–4006. https://doi.org/10.3390/molecules17043989
  • Bizimbitkiler. https://bizimbitkiler.org.tr/yeni/demos/technical. (Alınma Tarihi: 13.12.2024).
  • Bulatovic, V. M., Menkovic, N. R., Vajs, V. E., Milosavijevic, S. M. & Djokovic, D. D. (1997). Essential oil of Anthemis carpatica. Journal of Essential Oil Research 9(4), 397-400. https://doi.org/10.1080/ 10412905.1997.9700739
  • Bulatovic, V. M., Menkovic, N. R., Vajs, V. E., Milosavijevic, S. M. & Djokovic, D. D. (1998). Essential oil of Anthemis montana. Journal of Essential Oil Research 10(2), 223-226. https://doi.org/10.1080/10412905. 1998.9700887
  • Burt, S. (2004). Essential oils: Their antibacterial properties and potential applications in foods—A review. International Journal of Food Microbiology 94(3), 223–253. https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
  • CLSI (NCCLS) M7-A7 (2006). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard, seventh edition, Wayne, USA.
  • Davis, P. H. (1975). Flora of Türkiye and the East Aegean Islands, Vol. 5. Edinburgh, UK: Edinburgh University Press.
  • Demirci, B., Kırcı, D., Öztürk, G., & Demirci, F. (2022). Effect of Extraction Time on Origanum onites L. Infusions and Essential Oils–Biological Evaluation, Statistical Principal Component and Hierarchial Cluster Analyses. Chemistry & Biodiversity 19(12), e202200482. https://doi.org/10.1002/cbdv.202200482
  • Demirci, F., Güven, K., Demirci, B., Dadandi, M. Y., & Baser, K. H. C. (2008). Antibacterial activity of two Phlomis essential oils against food pathogens. Food control 19(12), 1159-1164. https://doi.org/10.1016/j.foodcont.2008.01.001
  • Formisano, C., Rigano, D., Senatore, F., Raimondo, F. M., Maggio, A. & Bruno, M. (2012). Essential oil composition and antibacterial activity of Anthemis mixta and A. tomentosa (Asteraceae). Natural Product Communications 7(10), 1934578X1200701035. https://doi.org/10.1177/1934578X1200701035
  • Güner, A., Özhatay, N., Ekim, T. & Baser, K. H. C. (2000). Flora of Türkiye and the East Aegean Islands, Vol. 11, University Press, Edinburgh.
  • Hamzaoğulu, E., Budak, Ü. & Koç, M.A. (2011). new taxon of Anthemis L. (Asteraceae) from Türkiye: Anthemis pauciloba Boiss. var. alba Hamzaoğlu & Budak var. nova. Turkish Journal of Botany 35, 85-88. https://doi.org/10.3906/bot-1003-15
  • Keskin, E. B., Servi, H. & Çelik, S. (2017). Essential oil composition and fatty acid profile of Cota tinctoria subsp. euxina (Boiss.) Oberpr. & Greuter and endemic Anthemis pauciloba Boiss. var. sieheana (Eig) Grierson from Türkiye. Natural Volatiles & Essential Oils 4(3), 112-113.
  • Kürkçüoğlu, M., Dualı, G., Duran, A. & Başer, K. H. C. (2009). The essential oil composition of Anthemis pauciloba 80155. var. microstephana (eig.) Grierson and Anthemis pauciloba var. pauciloba (Poster bildiri). 8th International Symposium on the Chemistry of Natural Compounds, Eskisehir, Türkiye, 15-17 June 2009, ss.152.
  • Kurtulmus, A., Fafal, T., Mert, T., Saglam, H., Kivcak, B., Ozturk, T., Demirci, B. & Baser, K. H. C. (2009). Chemical composition and antimicrobial activity of the essential oils of three Anthemis species from Türkiye. Chemistry of Natural Compounds 45, 900-904.
  • Melikoğlu, G., Kurtoğlu, S. & Kültür, Ş. (2015). Türkiye’de astım tedavisinde geleneksel olarak kullanılan bitkiler. Marmara Pharmaceutical Journal 19, 1-11. https://doi.org/10.12991/mpj.2015198604
  • Mighri, H., Hajlaoui, H., Akrout, A., Najjaa, H. & Neffati, M. (2010). Antimicrobial and antioxidant activities of Artemisia herba-alba essential oil cultivated in Tunisian arid zone. Comptes Rendus Chimie 13(3), 380-386. https://doi.org/10.1016/j.crci.2009.09.008
  • NIST Chemistry WebBook. https://webbook.nist.gov/(Alınma Tarihi: 27.03.2025).
  • Orlando, G., Zengin, G., Ferrante, C., Ronci, M., Recinella, L., Senkardes, I., Gevrenova, R., Zheleva-Dimitrova, D., Chiavaroli, A., Leone, S., Simone, S., Brunetti, L., Picot-Allain, C. M. N., Mahomoodally, M. F., Sinan, K. I. & Menghini, L. (2019). Comprehensive chemical profiling and multidirectional biological investigation of two wild Anthemis species (Anthemis tinctoria var. pallida and A. cretica subsp. tenuiloba): focus on neuroprotective effects. Molecules 24(14), 2582. https://doi.org/10.3390/molecules24142582
  • Özbek, M. F., Duman, H., Özbek, F. & Aytaç, Z. (2021). Anthemis ekicii (Asteraceae), a new species from Türkiye. Turkish Journal of Botany 45, 59-68. https://doi.org/10.3906/bot-2009-38
  • Pavlović, M., Lakušić, D., Kovačević, N., Tzakou, O. & Couladis, M. (2010). Comparative analysis of essential oils of six Anthemis taxa from Serbia and Montenegro. Chemistry & Biodiversity 7(5), 1231-1244. https://doi.org/10.1002/cbdv.200900156
  • Pelkonen, O., Abass, K. & Wiesner, J. (2013). Thujone and thujone-containing herbal medicinal and botanical products: Toxicological assessment. Regulatory Toxicology and Pharmacology 65(1), 100-107. https://doi.org/10.1016/j.yrtph.2012.11.002
  • The pherobase. http://www.pherobase.com/database/kovats/kovatsdetailsulcatone.php (Alınma Tarihi: 27.03.2025).
  • Puchem. https://pubchem.ncbi.nlm.nih.gov/compound/Isocaryophyllene-oxide#section=Computed-Properties (Alınma Tarihi: 27.03.2025).
  • Radulović, N. S., Genčić, M. S., Stojanović, N. M., Randjelović, P. J., Stojanović-Radić, Z. Z. & Stojiljković, N. I. (2017). Toxic essential oils. Part V: Behaviour modulating and toxic properties of thujones and thujone-containing essential oils of Salvia officinalis L., Artemisia absinthium L., Thuja occidentalis L. and Tanacetum vulgare L. Food and Chemical Toxicology 105, 355-369. https://doi.org/10.1016/j.fct.2017.04.044
  • Saltan, N., Kırcı, D., Köse, Y.B. & Demirci, B. (2018). Chemical compositions and antimicrobial activity of Prunella vulgaris L. International Journal of Agriculture Environment and Food Sciences 2(1), 186-189. https://doi.org/10.31015/jaefs.18032
  • Saroglou, V., Dorizas, N., Kypriotakis, Z. & Skaltsa, H. D. (2006). Analysis of the essential oil composition of eight Anthemis species from Greece. Journal of Chromatography A 1104, 313–322. https://doi.org/10.1016/ j.chroma.2005.11.087
  • Zámboriné Németh, É. & Thi Nguyen, H. (2020). Thujone, a widely debated volatile compound: What do we know about it? Phytochemistry Reviews 19, 405-423. https://doi.org/10.1007/s11101-020-09671-y

Türkiye'den Anthemis pauciloba Boiss. var. pauciloba'nın Uçucu Yağının Kimyasal Bileşimi ve Antimikrobiyal Etkisi

Year 2025, Volume: 28 Issue: 3, 617 - 624

Damla Kırcı , Ayla Kaya , Betül Demirci , Süleyman Doğu

https://doi.org/10.18016/ksutarimdoga.vi.1402876

Abstract

Anthemis pauciloba Boiss. var. pauciloba, Türkiye’de tanımlanan dört varyeteden biridir ve halk arasında “bol papatya” olarak bilinmektedir. Bitkinin çiçekleri, astım tedavisinde soğuk çay şeklinde kullanılmaktadır. Araştırmanın amacı, A. pauciloba var. pauciloba'nın toprak üstü kısımlarından Clevenger tipi aparat kullanılarak hidrodistilasyon yöntemiyle elde edilen uçucu yağın (EO) kimyasal bileşimini belirlemektir. Uçucu yağın bileşenleri, GC-FID ve GC-MS teknikleriyle eşzamanlı olarak analiz edilmiştir. Uçucu yağının mikrobiyal suşlara karşı antibakteriyel ve antifungal aktivitesini mikrodilüsyon tekniği kullanarak değerlendirmiştir. Uçucu yağın ana bileşikleri olarak α-tuyon (%28.7), α-pinen (%26.7) ve β-tuyon (%9.0) bulunmuştur. Gram-negatif, gram-pozitif ve mayaya karşı antimikrobiyal aktivite (Minimum İnhibitör Konsantrasyon) değerlendirilmiştir. Uçucu yağ, Candida krusei'ye karşı en yüksek antimikrobiyal aktiviteyi göstermiştir (MİK: 1.25 mg/mL). Bu çalışmada, A. pauciloba var. pauciloba’nın toprak üstü kısımlarından elde edilen uçucu yağın antimikrobiyal aktivitesi ilk kez değerlendirilmiştir.

Keywords

Anthemis pauciloba var. pauciloba Asteraceae Uçucu yağ Antimikrobiyal aktivite

References

  • Babushok, V. I., Linstrom, P. J., & Zenkevich, I. G. (2011). Retention indices for frequently reported compounds of plant essential oils. Journal of Physical and Chemical Reference Data, 40(1), 1–47. https://doi.org/ 10.1063/1.3653552
  • Bassolé, I. H. N., & Juliani, H. R. (2012). Essential oils in combination and their antimicrobial properties. Molecules 17(4), 3989–4006. https://doi.org/10.3390/molecules17043989
  • Bizimbitkiler. https://bizimbitkiler.org.tr/yeni/demos/technical. (Alınma Tarihi: 13.12.2024).
  • Bulatovic, V. M., Menkovic, N. R., Vajs, V. E., Milosavijevic, S. M. & Djokovic, D. D. (1997). Essential oil of Anthemis carpatica. Journal of Essential Oil Research 9(4), 397-400. https://doi.org/10.1080/ 10412905.1997.9700739
  • Bulatovic, V. M., Menkovic, N. R., Vajs, V. E., Milosavijevic, S. M. & Djokovic, D. D. (1998). Essential oil of Anthemis montana. Journal of Essential Oil Research 10(2), 223-226. https://doi.org/10.1080/10412905. 1998.9700887
  • Burt, S. (2004). Essential oils: Their antibacterial properties and potential applications in foods—A review. International Journal of Food Microbiology 94(3), 223–253. https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
  • CLSI (NCCLS) M7-A7 (2006). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard, seventh edition, Wayne, USA.
  • Davis, P. H. (1975). Flora of Türkiye and the East Aegean Islands, Vol. 5. Edinburgh, UK: Edinburgh University Press.
  • Demirci, B., Kırcı, D., Öztürk, G., & Demirci, F. (2022). Effect of Extraction Time on Origanum onites L. Infusions and Essential Oils–Biological Evaluation, Statistical Principal Component and Hierarchial Cluster Analyses. Chemistry & Biodiversity 19(12), e202200482. https://doi.org/10.1002/cbdv.202200482
  • Demirci, F., Güven, K., Demirci, B., Dadandi, M. Y., & Baser, K. H. C. (2008). Antibacterial activity of two Phlomis essential oils against food pathogens. Food control 19(12), 1159-1164. https://doi.org/10.1016/j.foodcont.2008.01.001
  • Formisano, C., Rigano, D., Senatore, F., Raimondo, F. M., Maggio, A. & Bruno, M. (2012). Essential oil composition and antibacterial activity of Anthemis mixta and A. tomentosa (Asteraceae). Natural Product Communications 7(10), 1934578X1200701035. https://doi.org/10.1177/1934578X1200701035
  • Güner, A., Özhatay, N., Ekim, T. & Baser, K. H. C. (2000). Flora of Türkiye and the East Aegean Islands, Vol. 11, University Press, Edinburgh.
  • Hamzaoğulu, E., Budak, Ü. & Koç, M.A. (2011). new taxon of Anthemis L. (Asteraceae) from Türkiye: Anthemis pauciloba Boiss. var. alba Hamzaoğlu & Budak var. nova. Turkish Journal of Botany 35, 85-88. https://doi.org/10.3906/bot-1003-15
  • Keskin, E. B., Servi, H. & Çelik, S. (2017). Essential oil composition and fatty acid profile of Cota tinctoria subsp. euxina (Boiss.) Oberpr. & Greuter and endemic Anthemis pauciloba Boiss. var. sieheana (Eig) Grierson from Türkiye. Natural Volatiles & Essential Oils 4(3), 112-113.
  • Kürkçüoğlu, M., Dualı, G., Duran, A. & Başer, K. H. C. (2009). The essential oil composition of Anthemis pauciloba 80155. var. microstephana (eig.) Grierson and Anthemis pauciloba var. pauciloba (Poster bildiri). 8th International Symposium on the Chemistry of Natural Compounds, Eskisehir, Türkiye, 15-17 June 2009, ss.152.
  • Kurtulmus, A., Fafal, T., Mert, T., Saglam, H., Kivcak, B., Ozturk, T., Demirci, B. & Baser, K. H. C. (2009). Chemical composition and antimicrobial activity of the essential oils of three Anthemis species from Türkiye. Chemistry of Natural Compounds 45, 900-904.
  • Melikoğlu, G., Kurtoğlu, S. & Kültür, Ş. (2015). Türkiye’de astım tedavisinde geleneksel olarak kullanılan bitkiler. Marmara Pharmaceutical Journal 19, 1-11. https://doi.org/10.12991/mpj.2015198604
  • Mighri, H., Hajlaoui, H., Akrout, A., Najjaa, H. & Neffati, M. (2010). Antimicrobial and antioxidant activities of Artemisia herba-alba essential oil cultivated in Tunisian arid zone. Comptes Rendus Chimie 13(3), 380-386. https://doi.org/10.1016/j.crci.2009.09.008
  • NIST Chemistry WebBook. https://webbook.nist.gov/(Alınma Tarihi: 27.03.2025).
  • Orlando, G., Zengin, G., Ferrante, C., Ronci, M., Recinella, L., Senkardes, I., Gevrenova, R., Zheleva-Dimitrova, D., Chiavaroli, A., Leone, S., Simone, S., Brunetti, L., Picot-Allain, C. M. N., Mahomoodally, M. F., Sinan, K. I. & Menghini, L. (2019). Comprehensive chemical profiling and multidirectional biological investigation of two wild Anthemis species (Anthemis tinctoria var. pallida and A. cretica subsp. tenuiloba): focus on neuroprotective effects. Molecules 24(14), 2582. https://doi.org/10.3390/molecules24142582
  • Özbek, M. F., Duman, H., Özbek, F. & Aytaç, Z. (2021). Anthemis ekicii (Asteraceae), a new species from Türkiye. Turkish Journal of Botany 45, 59-68. https://doi.org/10.3906/bot-2009-38
  • Pavlović, M., Lakušić, D., Kovačević, N., Tzakou, O. & Couladis, M. (2010). Comparative analysis of essential oils of six Anthemis taxa from Serbia and Montenegro. Chemistry & Biodiversity 7(5), 1231-1244. https://doi.org/10.1002/cbdv.200900156
  • Pelkonen, O., Abass, K. & Wiesner, J. (2013). Thujone and thujone-containing herbal medicinal and botanical products: Toxicological assessment. Regulatory Toxicology and Pharmacology 65(1), 100-107. https://doi.org/10.1016/j.yrtph.2012.11.002
  • The pherobase. http://www.pherobase.com/database/kovats/kovatsdetailsulcatone.php (Alınma Tarihi: 27.03.2025).
  • Puchem. https://pubchem.ncbi.nlm.nih.gov/compound/Isocaryophyllene-oxide#section=Computed-Properties (Alınma Tarihi: 27.03.2025).
  • Radulović, N. S., Genčić, M. S., Stojanović, N. M., Randjelović, P. J., Stojanović-Radić, Z. Z. & Stojiljković, N. I. (2017). Toxic essential oils. Part V: Behaviour modulating and toxic properties of thujones and thujone-containing essential oils of Salvia officinalis L., Artemisia absinthium L., Thuja occidentalis L. and Tanacetum vulgare L. Food and Chemical Toxicology 105, 355-369. https://doi.org/10.1016/j.fct.2017.04.044
  • Saltan, N., Kırcı, D., Köse, Y.B. & Demirci, B. (2018). Chemical compositions and antimicrobial activity of Prunella vulgaris L. International Journal of Agriculture Environment and Food Sciences 2(1), 186-189. https://doi.org/10.31015/jaefs.18032
  • Saroglou, V., Dorizas, N., Kypriotakis, Z. & Skaltsa, H. D. (2006). Analysis of the essential oil composition of eight Anthemis species from Greece. Journal of Chromatography A 1104, 313–322. https://doi.org/10.1016/ j.chroma.2005.11.087
  • Zámboriné Németh, É. & Thi Nguyen, H. (2020). Thujone, a widely debated volatile compound: What do we know about it? Phytochemistry Reviews 19, 405-423. https://doi.org/10.1007/s11101-020-09671-y
There are 29 citations in total.

Details

Primary Language English
Subjects Pharmaceutical Botany
Journal Section RESEARCH ARTICLE
Authors

Damla Kırcı 0000-0002-3479-3999

Ayla Kaya 0000-0002-7598-7132

Betül Demirci 0000-0003-2343-746X

Süleyman Doğu 0000-0002-5352-9288

Early Pub Date May 1, 2025
Publication Date
Submission Date December 29, 2024
Acceptance Date April 17, 2025
Published in Issue Year 2025Volume: 28 Issue: 3

Cite

APA Kırcı, D., Kaya, A., Demirci, B., Doğu, S. (2025). Chemical Composition and Antimicrobial Effect of Essential Oil of Anthemis pauciloba Boiss. var. pauciloba from Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(3), 617-624. https://doi.org/10.18016/ksutarimdoga.vi.1402876
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