Research Article
The Determination of In vitro Antioxidant and Cytotoxic Activities of Resin Obtained from Cilician Fir (Abies cilicica (Antoine & Kotschy) Carrière)
Abstract
In this study, the antioxidant and cytotoxic activities of resin obtained from the Cilician Fir plant were evaluated. According to data, the in vitro antioxidant activity of the resin has changed depending on the radical scavenging activity method. This resin has antioxidant activity according to 2,2′-Azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay. The in vitro cytotoxic activity of the resin was investigated against a panel of human cancer cells (MDA-MB-231, Hep G2, PC-3, U-87, MCF-7, HT-29) with the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay for 48 h. Normal human lung fibroblast cells (WI-38) were used as healthy cells. The results indicated that the in vitro cytotoxic activity of the resin depends on the cell line type and concentration of the resin. According to the IC50 values, the resin has the most cytotoxic activity on endometrial adenocarcinoma cancer cells (IC50=8.94 ± 0.03 µg mL-1) compared to other cancer cells. The results also indicated that Ishikawa endometrial adenocarcinoma cells, which have Selectivity Index (SI) value >2, have the most sensitivity against the resin. This study provides the first evidence that the resin inhibits the different cancer cells' growth.
Keywords
Thanks
The authors would like to acknowledge the contribution of the late Hanife Öğütlü for plant resin support in the realization of these studies. We hope that she rests in peace. Cytotoxic activity studies were conducted at Sivas Cumhuriyet University Advanced Technology Application and Research Center (CUTAM).
References
- Albanese L, Bonetti A, D’Acqui LP, Meneguzzo F, Zabini F 2019. Affordable Production of Antioxidant Aqueous Solutions by Hydrodynamic Cavitation Processing of Silver Fir (Abies alba Mill.) Needles. Foods. 18(2): pii: E65. https://doi.org/10.3390/foods8020065
- Albayrak S, Sağdıç O, Aksoy A 2010. Bitkisel ürünlerin ve gıdaların antioksidan kapasitelerinin belirlenmesinde kullanılan yöntemler. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 26(4): 401-409.
- Arslan BA, Ozen F, Catal T, Akalin E 2019. Resin extract obtained from Cilician fir (Abies Cilicica) inhibits glucose dependent inflammation in vitro. J Exp Ther Oncol,13(1):23-31.
- Broznić D, Ratkaj I, Staver MM, Pavelić SK, Žurga P, Bubalo D, Gobin I 2018. Evaluation of the Antioxidant Capacity, Antimicrobial and Antiproliferative Potential of Fir (Abies alba Mill.) Honeydew Honey Collected from Gorski kotar (Croatia). Food Technology and Biotechnology, 56(4: 533-545.
- Camarda L, Stefano VD, Pitonzo R 2011. Natural resins: Chemical constituents and medicinal uses. Book Chapter, Natural Resins, Chemical constituents and medicinal uses nova science in resin composites properties, production and applications, 1-27.
- Chun SS, Vattem DA, Lin YT, Shetty K 2005. Phenolic antioxidants from clonal oregano (Origanum vulgare) with antimicrobial activity against Helicobacter pylori. Process Biochem, 40, 809-816.
- Clarke G, Ting KN, Wiart C, Fry J 2013. High correlation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the Malaysian rainforest. Antioxidants (Basel), 2:1-10.
- Davis PH 1967. Flora of Turkey and The Esat Aegean Islands, Vol 9, Edinburg University Press, Edinburg.
- Dayısoylı KS, Mehmet HA 2009. Chemical analysis of essential oils from cone’s rosin of Cilician fir (Abies cilicica subsp. cilicica). African Journal of Biotechnology, 8 (15): 3502-3505.
- Dayisoylu SK, Duman AD, Hakki Alma M, Digrak M 2009. Antimicrobial activity of the essential oils of rosin from cones of Abies cilicica subsp. cilicica. Afr J Biotechn, 8: 5021-24.
- Do JR, Kang SN, Kim K.J, Jo JH, Lee SW 2004. Antimicrobial and antixidant activities and phenolic contents in the water extract of medicinal plants. Food Sci. Biotechnol, 13: 640-645.
- Duthie SJ, Collins AR, Duthie GG, Dobson VL 1997. Quercetin and myricetin protect against hydrogen peroxide-induced DNA damage (strand breaks and oxidised pyrimidines) in human lymphocytes. Mutat Res, 393:223–331.
- Evans MD, Dizdaroglu M, Cooke MS 2004. Oxidative DNA damage and disease: induction, repair and significance. Mutation Research, 567(1): 1-61.
- Fujita T, Sezik E, Tabata M, Yesilada E, Honda G, Takeda Y, Tanaka T, Takaishi Y 1995. Traditional medicine in Turkey. Ⅶ. Folk medicine in middle and west Black Sea regions. Econ. Bot, 4: 406-422.
- Handa M, Murata T, Kobayashi K, Selenge E, Miyase T, Batkhuu J, Yoshizaki F 2013. Lipase inhibitory and LDL anti-oxidative triterpenes from Abies sibirica. Phytochemistry, 86: 168-175.
- Hasegawa S, Miura T, Hirose Y, Iitaka Y 1985. A new rearranged lanostanoid, mariesiic acid A, from the seed of Abies mariesii. Chem. Lett, 14(10): 1589-1592.
- Kilic Pekgözlü A, Ceylan E 2018. Chemical Compostion of Taurus Fir (Abies cilicica subsp. isaurica) Oleoresin. Revista Árvore, 42(1): e420115. http://dx.doi.org/10.1590/1806-90882018000100015.
- Kizil M, Kizil G, Yavuz M, Aytekin C 2002. Antimicrobial Activity of Resins Obtained from the Roots and Stems of Cedrus libani and Abies Cilicia. Appl Biochem Microbiol, 38: 144-6.
- Kunyanga CN, Imungi JK, Okoth MW, Biesalski HK, Vadivel V 2012. Total Phenolic Content, Antioxidant and Antidiabetic Properties of Methanolic Extract of Raw and Traditionally Processed Kenyan Indigenous Food Ingredients. LWT - Food Sci. Technol, 45: 269-276.
- Lavoie S, Gauthier C, Legault J, Mercier S, Mshvildadze V, Pichette A 2013. Lanostane- and cycloartane-type triterpenoids from Abies balsamea oleoresin. Beilstein J. Org. Chem, 9: 1333-1339.
- Li YL, Gao YX, Jin HZ, Shan L, Chang WL, Yang XW, Zeng HW, Wang N, Steinmetz A, Zhang WD 2015. Chemical constituents of Abies fabri. Phytochemistry, 117: 135-143.
- Pohanka M 2011. Cholinesterases, A Target of Pharmacology and Toxicology. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 155(3): 219-230.
- Saddiqe Z, Naeem I, Maimoona A 2010. A review of the antibacterial activity of Hypericum perforatum L. J Ethnopharmacol, 131(3): 511-521. Singh RK, Bhattacharya SK, Acharya SB 2000. Pharmacological activity of Abies pindrow. J Ethnopharmacol, 73(1-2): 47-51.
- Silva S, Gomes L, Leitão F, Coelho AV, Vilas Boas, L 2006. Phenolic compounds and antioxidant activity of Olea europaea L. fruit and leaves. Food Scı Technol Int, 12: 385- 396.
- Skaper SD, Fabris M, Ferrari V, DalleCarbonare M, Leon A 1997. Quercetin protects cutaneous tissue-associated cell types including sensory neurons from oxidative stress induced by glutathione depletion: cooperative effects of ascorbic acid. Free Radic Biol Med, 22:669–678.
- Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, Warren JT, Bokesch H, Kenney S, Boyd MR 1990. New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst, 82(13): 1107-1112.
- Tumen I, Akkol EK, Süntar I, Keleş H 2011. Wound repair and anti-inflammatory potential of essential oils from cones of Pinaceae: preclinical experimental research in animal models. J Ethnopharmacol, 137(3):1215-20.
- Wang GW, Lv C, Fang X, Tian XH, Ye J, Li HL, Shan L, Shen YH, Zhang WD 2015. Eight pairs of epimeric triterpenoids involving a characteristic spiro-E/F ing from Abies faxoniana. J. Nat. Prod, 78(1): 50-60.
- Waris G, Ahsan H 2006. Reactive oxygen species: role in the development of cancer and various chronic conditions. J Carcinog, 5: 14.
- Vasincu A, Creţu E, Geangalău I, Mihăilescu Amalinei RL , Miron A, 2013. Polyphenolic Content And Antioxidant Activity Of An Extractive Fraction From Abies Alba Bark. Rev. Med. Chir. Soc. Med. Nat., Iaşi, 117(2):545-50.
- Wu W, Chen X, Liu Y, Wang Y, Tian T, Zhao X, Li J, Ruan H 2016. Triterpenoids from the branch and leaf of Abies fargesii. Phytochemistry, 130: 301-312.
- Yang H, Dong Y, Du H, Shi H, Peng Y, Li X 2011. Antioxidant compounds from propolis collected in Anhui, China. Molecules, 16:3444-3455.
- Yeşilada E, Honda G, Sezik E, Tabata M, Fujita T, Tanaka T, Takeda Y, Takaishi Y 1995. Traditional medicine in Turkey. V. Folk medicine in the inner Taurus Mountains. J Ethnopharmacol, 46(3): 133-52.
- Yeomans MR 1996. Palatability and The Micro-Structure of Feeding in Humans: The Appetizer Effect. Appetite, 27(2): 119–133.
Toros Göknarı (Abies cilicica (Antoine & Kotschy) Carrière) ’ndan Elde Edilen Reçinenin In vitro Antioksidan ve Sitotoksik Aktivitelerinin Belirlenmesi
Abstract
Bu çalışmada, Toros Göknarı bitkisinden elde edilen reçinenin antioksidan ve sitotoksik aktivite değerleri değerlendirilmiştir. Verilere göre, reçinenin in vitro antioksidan aktivitesi, radikal süpürücü aktivite yöntemine bağlı olarak değişmiştir. Bu reçine, 2,2′-Azino-bis- (3-etilbenzotiazolin-6-sülfonik asit) (ABTS) radikal süpürme testine göre antioksidan aktiviteye sahiptir. Reçinenin in vitro sitotoksik aktivitesi, bir dizi insan kanser hücresine (MDA-MB-231, Hep G2, PC-3, U-87, MCF-7, HT-29) karşı MTT (3- (4,5-dimetiltiyazol-2-il) -2,5-difeniltetrazolium bromid) analizi ile 48 saat boyunca araştırıldı. Sağlıklı hücreler olarak normal insan akciğer fibroblast hücreleri (WI-38) kullanıldı. Sonuçlar, reçinenin in vitro sitotoksik aktivitesinin hücre hattı tipine ve numunenin konsantrasyonuna bağlı olduğunu göstermiştir. IC50 değerlerine göre, reçine diğer kanser hücrelerine kıyasla endometriyal adenokarsinom kanser hücreleri üzerinde en fazla sitotoksik aktiviteye sahiptir (IC50 = 8.94 ± 0.03 µg mL-1). Sonuçlar ayrıca seçicilik indeks (SI) değeri> 2 olan Ishikawa endometriyal adenokarsinom hücrelerinin reçineye karşı en yüksek hassasiyete sahip olduğunu göstermiştir. Bu çalışma, reçinenin farklı kanser hücrelerinin büyümesini engellediğine dair ilk kanıtları sunmaktadır.
Keywords
References
- Albanese L, Bonetti A, D’Acqui LP, Meneguzzo F, Zabini F 2019. Affordable Production of Antioxidant Aqueous Solutions by Hydrodynamic Cavitation Processing of Silver Fir (Abies alba Mill.) Needles. Foods. 18(2): pii: E65. https://doi.org/10.3390/foods8020065
- Albayrak S, Sağdıç O, Aksoy A 2010. Bitkisel ürünlerin ve gıdaların antioksidan kapasitelerinin belirlenmesinde kullanılan yöntemler. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 26(4): 401-409.
- Arslan BA, Ozen F, Catal T, Akalin E 2019. Resin extract obtained from Cilician fir (Abies Cilicica) inhibits glucose dependent inflammation in vitro. J Exp Ther Oncol,13(1):23-31.
- Broznić D, Ratkaj I, Staver MM, Pavelić SK, Žurga P, Bubalo D, Gobin I 2018. Evaluation of the Antioxidant Capacity, Antimicrobial and Antiproliferative Potential of Fir (Abies alba Mill.) Honeydew Honey Collected from Gorski kotar (Croatia). Food Technology and Biotechnology, 56(4: 533-545.
- Camarda L, Stefano VD, Pitonzo R 2011. Natural resins: Chemical constituents and medicinal uses. Book Chapter, Natural Resins, Chemical constituents and medicinal uses nova science in resin composites properties, production and applications, 1-27.
- Chun SS, Vattem DA, Lin YT, Shetty K 2005. Phenolic antioxidants from clonal oregano (Origanum vulgare) with antimicrobial activity against Helicobacter pylori. Process Biochem, 40, 809-816.
- Clarke G, Ting KN, Wiart C, Fry J 2013. High correlation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the Malaysian rainforest. Antioxidants (Basel), 2:1-10.
- Davis PH 1967. Flora of Turkey and The Esat Aegean Islands, Vol 9, Edinburg University Press, Edinburg.
- Dayısoylı KS, Mehmet HA 2009. Chemical analysis of essential oils from cone’s rosin of Cilician fir (Abies cilicica subsp. cilicica). African Journal of Biotechnology, 8 (15): 3502-3505.
- Dayisoylu SK, Duman AD, Hakki Alma M, Digrak M 2009. Antimicrobial activity of the essential oils of rosin from cones of Abies cilicica subsp. cilicica. Afr J Biotechn, 8: 5021-24.
- Do JR, Kang SN, Kim K.J, Jo JH, Lee SW 2004. Antimicrobial and antixidant activities and phenolic contents in the water extract of medicinal plants. Food Sci. Biotechnol, 13: 640-645.
- Duthie SJ, Collins AR, Duthie GG, Dobson VL 1997. Quercetin and myricetin protect against hydrogen peroxide-induced DNA damage (strand breaks and oxidised pyrimidines) in human lymphocytes. Mutat Res, 393:223–331.
- Evans MD, Dizdaroglu M, Cooke MS 2004. Oxidative DNA damage and disease: induction, repair and significance. Mutation Research, 567(1): 1-61.
- Fujita T, Sezik E, Tabata M, Yesilada E, Honda G, Takeda Y, Tanaka T, Takaishi Y 1995. Traditional medicine in Turkey. Ⅶ. Folk medicine in middle and west Black Sea regions. Econ. Bot, 4: 406-422.
- Handa M, Murata T, Kobayashi K, Selenge E, Miyase T, Batkhuu J, Yoshizaki F 2013. Lipase inhibitory and LDL anti-oxidative triterpenes from Abies sibirica. Phytochemistry, 86: 168-175.
- Hasegawa S, Miura T, Hirose Y, Iitaka Y 1985. A new rearranged lanostanoid, mariesiic acid A, from the seed of Abies mariesii. Chem. Lett, 14(10): 1589-1592.
- Kilic Pekgözlü A, Ceylan E 2018. Chemical Compostion of Taurus Fir (Abies cilicica subsp. isaurica) Oleoresin. Revista Árvore, 42(1): e420115. http://dx.doi.org/10.1590/1806-90882018000100015.
- Kizil M, Kizil G, Yavuz M, Aytekin C 2002. Antimicrobial Activity of Resins Obtained from the Roots and Stems of Cedrus libani and Abies Cilicia. Appl Biochem Microbiol, 38: 144-6.
- Kunyanga CN, Imungi JK, Okoth MW, Biesalski HK, Vadivel V 2012. Total Phenolic Content, Antioxidant and Antidiabetic Properties of Methanolic Extract of Raw and Traditionally Processed Kenyan Indigenous Food Ingredients. LWT - Food Sci. Technol, 45: 269-276.
- Lavoie S, Gauthier C, Legault J, Mercier S, Mshvildadze V, Pichette A 2013. Lanostane- and cycloartane-type triterpenoids from Abies balsamea oleoresin. Beilstein J. Org. Chem, 9: 1333-1339.
- Li YL, Gao YX, Jin HZ, Shan L, Chang WL, Yang XW, Zeng HW, Wang N, Steinmetz A, Zhang WD 2015. Chemical constituents of Abies fabri. Phytochemistry, 117: 135-143.
- Pohanka M 2011. Cholinesterases, A Target of Pharmacology and Toxicology. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 155(3): 219-230.
- Saddiqe Z, Naeem I, Maimoona A 2010. A review of the antibacterial activity of Hypericum perforatum L. J Ethnopharmacol, 131(3): 511-521. Singh RK, Bhattacharya SK, Acharya SB 2000. Pharmacological activity of Abies pindrow. J Ethnopharmacol, 73(1-2): 47-51.
- Silva S, Gomes L, Leitão F, Coelho AV, Vilas Boas, L 2006. Phenolic compounds and antioxidant activity of Olea europaea L. fruit and leaves. Food Scı Technol Int, 12: 385- 396.
- Skaper SD, Fabris M, Ferrari V, DalleCarbonare M, Leon A 1997. Quercetin protects cutaneous tissue-associated cell types including sensory neurons from oxidative stress induced by glutathione depletion: cooperative effects of ascorbic acid. Free Radic Biol Med, 22:669–678.
- Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, Warren JT, Bokesch H, Kenney S, Boyd MR 1990. New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst, 82(13): 1107-1112.
- Tumen I, Akkol EK, Süntar I, Keleş H 2011. Wound repair and anti-inflammatory potential of essential oils from cones of Pinaceae: preclinical experimental research in animal models. J Ethnopharmacol, 137(3):1215-20.
- Wang GW, Lv C, Fang X, Tian XH, Ye J, Li HL, Shan L, Shen YH, Zhang WD 2015. Eight pairs of epimeric triterpenoids involving a characteristic spiro-E/F ing from Abies faxoniana. J. Nat. Prod, 78(1): 50-60.
- Waris G, Ahsan H 2006. Reactive oxygen species: role in the development of cancer and various chronic conditions. J Carcinog, 5: 14.
- Vasincu A, Creţu E, Geangalău I, Mihăilescu Amalinei RL , Miron A, 2013. Polyphenolic Content And Antioxidant Activity Of An Extractive Fraction From Abies Alba Bark. Rev. Med. Chir. Soc. Med. Nat., Iaşi, 117(2):545-50.
- Wu W, Chen X, Liu Y, Wang Y, Tian T, Zhao X, Li J, Ruan H 2016. Triterpenoids from the branch and leaf of Abies fargesii. Phytochemistry, 130: 301-312.
- Yang H, Dong Y, Du H, Shi H, Peng Y, Li X 2011. Antioxidant compounds from propolis collected in Anhui, China. Molecules, 16:3444-3455.
- Yeşilada E, Honda G, Sezik E, Tabata M, Fujita T, Tanaka T, Takeda Y, Takaishi Y 1995. Traditional medicine in Turkey. V. Folk medicine in the inner Taurus Mountains. J Ethnopharmacol, 46(3): 133-52.
- Yeomans MR 1996. Palatability and The Micro-Structure of Feeding in Humans: The Appetizer Effect. Appetite, 27(2): 119–133.
There are 34 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Structural Biology |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Publication Date | December 31, 2020 |
| Submission Date | April 16, 2020 |
| Acceptance Date | May 31, 2020 |
| Published in Issue | Year 2020 |
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