Öz
Geleneksel Çin tıbbında kullanılan Pawlonia tomentosa sahip olduğu biyolojik aktiviteler sayesinde bronşit ve astım gibi hastalıkların tedavisinde kullanılmaktadır. Bunun yanı sıra antimikrobiyal, antioksidan, antikanser gibi etkilerinin olduğu ve dizanteri, bel soğukluğu, hemoroid gibi hastalıkların tedavisinde de kullanıldığı bilinmektedir. Bu çalışmada P. tomentosa’nın çiçek, petal, kabuk, yaprak ve odun kısımlarının metanol çözücüsünden elde edilen ekstraklarının antimikrobiyal aktivitesi disk difüzyon metoduna göre belirlenmiştir. Çiçek ve petal kısımlarının metanolden elde edilen ekstraktının farklı konsantrasyonlarının antioksidan aktivitesi 2,2-difenil-1-pikrilhidrazil (DPPH) radikal süpürücü kapasitesi yöntemine göre tespit edilmiştir. Metanol, etanol ve hekzan gibi çözücülerden elde edilen ekstraktlarının farklı konsantrasyonlarının antikanser aktivitesi 3- (4,5-dimetiltiyazol-2-iil) -2,5-difenil tetrazolium bromür (MTT) test yöntemi kullanılarak belirlenmiştir. Sonuçta en iyi antimikrobiyal aktiviteyi P. tomentosa’nın çiçek kısmının metanol özütü S. aureus’a (18 mm) karşı gösterdiği tespit edilmiştir. P. tomentosa’nın antioksidan aktivitesinin artan konsantrasyonlara bağlı olarak arttığı belirlenmiştir. P. tomentosa’nın en iyi sitotoksik etkiyi hekzan ekstresinde gösterdiği tespit edilmiştir.
Anahtar Kelimeler
Tıbbi bitki Paulownia tomentosa Antimikrobiyal etki Antioksidan Etki Sitotoksik etki
Kaynakça
- Adıgüzel F, Kızılaslan N 2016. İstanbul ilinde tıbbi, aromatik ve baharat bitkileri satışı yapan aktarların yapısal özellikleri ve mevzuat hakkındaki görüşleri. Ana Tarım Bilim Derg 31:40-59.
- Atalan E, Bülbül AS, Ceylan Y 2020. Cephalarıa Syrıaca (L.): Investigation of antimicrobial, antibiofılm, antioxidant potential and seed morphology. Fresen Environ Bull 29(5):3641-3649.
- Bülbül AS, Ceylan Y, Armağan M 2018. Investigation of antibacterial and antifungal properties of Acanthophyllum acerosum and Acanthophyllum microcephalum. Re J Bio Sci 11(2): 14-17.
- Cercós AP 1982. Antimicrobial activity of the epicarp of the fruits of Paulownia fortunei and Paulownia tomentosa. Rev Argen de Microbio 14(2):111-114.
- Collins CM, Lyne PM 1987. Microbiological methods. Butterworths & Co. Ltd., London.
- Cuendet M, Hostettmann K, Potterat O, Dyatmiko W 1997. Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helvetica Chimica Acta 80(4):1144-1152.
- Davis PH 1970-1984-1985. Flora of Turkey and the Aegean Islands. V:7, 8, 9 Edinburgh Univ. Press. England.
- Doğan Ö, Avcı A 2018. Bitkilerle tedavi ve ilaç etkileşimleri. Turk Klin J Pub Heal 4(1): 49-54.
- Eisenberg DM, Davis RB, Ettner SL, Appel S, Wilkey S, Van Rompay M, Kessler RC 1998. Trends in alternative medicine use in the United States, 1990- 1997: results of a follow-up national survey. JAMA 280:1569-75.
- Faydaoğlu E, Sürücüoğlu MS 2011. Geçmisten Günümüze Tıbbi ve Aromatik Bitkilerin Kullanılması ve Ekonomik Önemi. KÜ Orman Fak Derg 11(1):52-67.
- Ji P, Chen C, Hu Y, Zhan Z, Pan W, Li R, Li E, Ge HM, Yang G 2015. Antiviral Activity of Paulownia tomentosa against Enterovirus 71 of Hand, Foot, and Mouth Disease. Biol Pharm Bull 38:1–6.
- Kang KH, Huh H, Kim BK, Lee CK 1999. An Antiviral Furanoquinone from Paulownia tomentosa Steud. Phytother Res 13:624–626.
- Kardaş C 2019. Muş'ta Yabani Bitkilerin Halk Hekimliğinde Kullanılması. Lokman Hekim Derg 9(1):85-96.
- King M, Pettigrew A 2003. Complemantary and alternative therapy use by older adults in three ethnically diverse populations: Apilot study. Geriatric Nursing 25(1): 30-7.
- Kirby AJ, Schmidt RJ 1997. The antioxidant activity of Chinese herbs for eczema and of placebo herbs. I. J Ethnopharma 56(2): 103-8.
- Lee JW, Seo KH, Ryu HW, Yuk HJ, Park HA, Lim YR, Ahn KS, Oh SR 2018. Anti-inflammatory effect of stem bark of Paulownia tomentosa Steud. inlipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and LPS- induced murine model of acute lung injury. J Ethnopharma 210:23–30.
- Mossman T 1983. Rapid Colorimetric Assay For Cellular Growth And Survival: Application To Proliferation And Cytotoxicity Assays. J Immunol Methods 65:55-63.
- Navrátilová A, Schneiderová K, Veselá D, Hanáková Z, Fontana A, Dall’acqua S, Cvacka J, Innocenti G, Novotná J, Urbanová M, Pelletier J, Cízek A, Zemlicková H, Šmejkal K 2013. Minor C-Geranylated Flavanones From Paulownia tomentosa Fruits With Mrsa Antibacterial Activity. Phytochem 89:104–113.
- Navrátilová A, Nešuta O, Vančatová I, Čížek A, Varela-M RE, López-Abán J, Villa-Pulgarin JA, Mollinedo F, Muro A, Žemličková H, Kadlecová D, Šmejkal K 2016. C-Geranylated Flavonoids From Paulownia tomentosa Fruits With Antimicrobial Potential And Synergistic Activity With Antibiotics. Pharma Bıo 54 (8):1398–1407.
- Popova TP, Baykov BD 2013. Antimicrobial Activity of Aqueous Extracts of Leaves and Silage from Paulownia elongata. American J Bio Chem and Pharma Sci 1(2):8–15.
- San Jose Mdel C, Cernadas MJ, Corredoira E 2014. Histology of the regeneration of Paulownia tomentosa (Paulowniaceae) by organogenesis. Rev De Bio Tropical 62:809-818.
- Šmejkal K, Chudík S, Kloucek P, Marek R, Cvacka J, Urbanová M, Julínek O, Kokoška L, Šlapetová T, Holubová P, Zima A, Dvorská M 2008. Antibacterial C-Geranyl flavonoids from Paulownia tomentosa Fruits. J Nat Prod 71:706–709.
- Smejkal K, Holubova P, Zima A, Muselik J, Dvorska M 2007. Antiradical Activity of Paulownia tomentosa (Scrophulariaceae) Extracts. Molecules 12:1210-1219.
- Tozyılmaz V, Bülbül AS, Ceylan Y, Armağan M 2020. Antibacterial, antifungal, antibiofilm and antioxidant activities of some endemic plants in Anatolian flora. Fresen Environ Bull 29(6):4338-4346.
- Uğuz Ö 2018. Denizli’de Yetişen Paulownia tomentosa Steud’nın Fitokimyasal Profilinin Ve Elektriksel İletkenliğinin Belirlenmesi. Pamukkale Üniversitesi Fen Bilimleri Enstitüsü Biyoloji Anabilim Dalı, Yüksek Lisans Tezi, 65 s.
- Uğuz Ö, Kara Y 2019. Determination of Antioxidant Potential in the Leaf and Flower of Paulownia tomentosa. In J Second Metabolite 6(2):106-112.
- Zima A, Hošek J, Treml J, Muselík J, Suchý P, Pražanová G, Lopes A, Žemlička M 2010. Antiradical and Cytoprotective Activities of Several C-Geranyl-substituted Flavanones from Paulownia tomentosa Fruit, Molecules 15:6035-6049
Determination of The Antimicrobial, Antioxidant and Cytotoxic Activity of Paulownia tomentosa Steud.
Öz
Paulownia tomentosa, used in traditional Chinese medicine, is used in the treatment of diseases such as bronchitis and asthma thanks to its biological activities. In addition, it is known to have antimicrobial, antioxidant, anticancer effects and it is also used in the treatment of diseases such as dysentery, gonorrhea, hemorrhoids. In this study, the antimicrobial activity of extracts obtained from methanol solvent of flower and petal parts of P. tomentosa was determined according to the disk diffusion method. The antioxidant activity of the different concentrations of the extracts obtained from methanol solvent of flower and petal extracts was determined via the 2,2-diphenyl-1-picrilhydrazyl (DPPH) radical scavenging capacity method. Anticancer activity of different concentrations of extracts obtained from solvents such as methanol, ethanol and hexane was determined using the 3- (4,5-dimethylthiazole-2-yl) -2,5-diphenyl tetrazolium bromide (MTT) test method. As a result, it was found that methanol extract of the flower part of P. tomentosa showed the best antimicrobial activity against S. aureus (18 mm). It was determined that antioxidant activity of P. tomentosa increased depend to increasing concentrations. It was concluded that P. tomentosa has the best cytotoxic effect in hexane extract.
Anahtar Kelimeler
Medical plant Paulownia tomentosa Antimicrobial effect Antioxidant effect Cytotoxic effect
Kaynakça
- Adıgüzel F, Kızılaslan N 2016. İstanbul ilinde tıbbi, aromatik ve baharat bitkileri satışı yapan aktarların yapısal özellikleri ve mevzuat hakkındaki görüşleri. Ana Tarım Bilim Derg 31:40-59.
- Atalan E, Bülbül AS, Ceylan Y 2020. Cephalarıa Syrıaca (L.): Investigation of antimicrobial, antibiofılm, antioxidant potential and seed morphology. Fresen Environ Bull 29(5):3641-3649.
- Bülbül AS, Ceylan Y, Armağan M 2018. Investigation of antibacterial and antifungal properties of Acanthophyllum acerosum and Acanthophyllum microcephalum. Re J Bio Sci 11(2): 14-17.
- Cercós AP 1982. Antimicrobial activity of the epicarp of the fruits of Paulownia fortunei and Paulownia tomentosa. Rev Argen de Microbio 14(2):111-114.
- Collins CM, Lyne PM 1987. Microbiological methods. Butterworths & Co. Ltd., London.
- Cuendet M, Hostettmann K, Potterat O, Dyatmiko W 1997. Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helvetica Chimica Acta 80(4):1144-1152.
- Davis PH 1970-1984-1985. Flora of Turkey and the Aegean Islands. V:7, 8, 9 Edinburgh Univ. Press. England.
- Doğan Ö, Avcı A 2018. Bitkilerle tedavi ve ilaç etkileşimleri. Turk Klin J Pub Heal 4(1): 49-54.
- Eisenberg DM, Davis RB, Ettner SL, Appel S, Wilkey S, Van Rompay M, Kessler RC 1998. Trends in alternative medicine use in the United States, 1990- 1997: results of a follow-up national survey. JAMA 280:1569-75.
- Faydaoğlu E, Sürücüoğlu MS 2011. Geçmisten Günümüze Tıbbi ve Aromatik Bitkilerin Kullanılması ve Ekonomik Önemi. KÜ Orman Fak Derg 11(1):52-67.
- Ji P, Chen C, Hu Y, Zhan Z, Pan W, Li R, Li E, Ge HM, Yang G 2015. Antiviral Activity of Paulownia tomentosa against Enterovirus 71 of Hand, Foot, and Mouth Disease. Biol Pharm Bull 38:1–6.
- Kang KH, Huh H, Kim BK, Lee CK 1999. An Antiviral Furanoquinone from Paulownia tomentosa Steud. Phytother Res 13:624–626.
- Kardaş C 2019. Muş'ta Yabani Bitkilerin Halk Hekimliğinde Kullanılması. Lokman Hekim Derg 9(1):85-96.
- King M, Pettigrew A 2003. Complemantary and alternative therapy use by older adults in three ethnically diverse populations: Apilot study. Geriatric Nursing 25(1): 30-7.
- Kirby AJ, Schmidt RJ 1997. The antioxidant activity of Chinese herbs for eczema and of placebo herbs. I. J Ethnopharma 56(2): 103-8.
- Lee JW, Seo KH, Ryu HW, Yuk HJ, Park HA, Lim YR, Ahn KS, Oh SR 2018. Anti-inflammatory effect of stem bark of Paulownia tomentosa Steud. inlipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and LPS- induced murine model of acute lung injury. J Ethnopharma 210:23–30.
- Mossman T 1983. Rapid Colorimetric Assay For Cellular Growth And Survival: Application To Proliferation And Cytotoxicity Assays. J Immunol Methods 65:55-63.
- Navrátilová A, Schneiderová K, Veselá D, Hanáková Z, Fontana A, Dall’acqua S, Cvacka J, Innocenti G, Novotná J, Urbanová M, Pelletier J, Cízek A, Zemlicková H, Šmejkal K 2013. Minor C-Geranylated Flavanones From Paulownia tomentosa Fruits With Mrsa Antibacterial Activity. Phytochem 89:104–113.
- Navrátilová A, Nešuta O, Vančatová I, Čížek A, Varela-M RE, López-Abán J, Villa-Pulgarin JA, Mollinedo F, Muro A, Žemličková H, Kadlecová D, Šmejkal K 2016. C-Geranylated Flavonoids From Paulownia tomentosa Fruits With Antimicrobial Potential And Synergistic Activity With Antibiotics. Pharma Bıo 54 (8):1398–1407.
- Popova TP, Baykov BD 2013. Antimicrobial Activity of Aqueous Extracts of Leaves and Silage from Paulownia elongata. American J Bio Chem and Pharma Sci 1(2):8–15.
- San Jose Mdel C, Cernadas MJ, Corredoira E 2014. Histology of the regeneration of Paulownia tomentosa (Paulowniaceae) by organogenesis. Rev De Bio Tropical 62:809-818.
- Šmejkal K, Chudík S, Kloucek P, Marek R, Cvacka J, Urbanová M, Julínek O, Kokoška L, Šlapetová T, Holubová P, Zima A, Dvorská M 2008. Antibacterial C-Geranyl flavonoids from Paulownia tomentosa Fruits. J Nat Prod 71:706–709.
- Smejkal K, Holubova P, Zima A, Muselik J, Dvorska M 2007. Antiradical Activity of Paulownia tomentosa (Scrophulariaceae) Extracts. Molecules 12:1210-1219.
- Tozyılmaz V, Bülbül AS, Ceylan Y, Armağan M 2020. Antibacterial, antifungal, antibiofilm and antioxidant activities of some endemic plants in Anatolian flora. Fresen Environ Bull 29(6):4338-4346.
- Uğuz Ö 2018. Denizli’de Yetişen Paulownia tomentosa Steud’nın Fitokimyasal Profilinin Ve Elektriksel İletkenliğinin Belirlenmesi. Pamukkale Üniversitesi Fen Bilimleri Enstitüsü Biyoloji Anabilim Dalı, Yüksek Lisans Tezi, 65 s.
- Uğuz Ö, Kara Y 2019. Determination of Antioxidant Potential in the Leaf and Flower of Paulownia tomentosa. In J Second Metabolite 6(2):106-112.
- Zima A, Hošek J, Treml J, Muselík J, Suchý P, Pražanová G, Lopes A, Žemlička M 2010. Antiradical and Cytoprotective Activities of Several C-Geranyl-substituted Flavanones from Paulownia tomentosa Fruit, Molecules 15:6035-6049
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Yapısal Biyoloji |
| Bölüm | ARAŞTIRMA MAKALESİ (Research Article) |
| Yazarlar | |
| Yayımlanma Tarihi | 31 Ağustos 2021 |
| Gönderilme Tarihi | 2 Ekim 2020 |
| Kabul Tarihi | 3 Aralık 2020 |
| Yayımlandığı Sayı | Yıl 2021 |
Cited By
2022-JIF = 0.500
2022-JCI = 0.170
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