Yıl 2020, Cilt 23 , Sayı 1, Sayfalar 7 - 18 2020-02-28

Sebzelerde Sorun Olan Önemli Bitki Fungal Hastalık Etmenlerine Karşı Vermikomposttan İzole Edilen Mikrobiyomların in vitro Antagonistik Etkilerinin Belirlenmesi
Determinations of in vitro Antagonistic Effects of Microbiomes Isolated from Vermicompost Against Major Plant Fungal Disease Agents of Vegetables

Emine Mine SOYLU [1] , Soner SOYLU [2] , Merve KARA [3] , Şener KURT [4]


Bu çalışmada, solucan gübresi olarak bilinen vermikompostlardan elde edilen bakteriyel mikrobiyomların sebzelerde sorun olan önemli yaprak ve toprak kökenli fungal hastalık etmenlerinden Sclerotinia sclerotiorum, Macrophomina phaseolina, Botrytis cinerea, Verticilium dahliae üzerine olan antagonistik etkinlikleri in vitro koşullarda araştırılmıştır. Yapılan izolasyonlar sonucunda toplam 69 adet aday bakteriyel izolat elde edilmiştir. Elde edilen bakteriyel izolatlar arasında 28 bakteriyel izolat (toplam izolatın %49.12) in vitro ikili kültür testlemelerinde fungal etmenlerden S. sclerotiorum’un gelişimini %1.72-75.43, M. phaseolina’nın gelişimini %1.67-65.83, B. cinerea gelişimini %3.44-57.18, V. dahliae gelişimini ise %2.28-58.74 gibi değişen oranlarda engellemişlerdir. Antagonist potansiyele sahip bakteriyel izolatların çoğunluğunun Bacillus spp.’e ait olduğu belirlenmiştir. İzolatlar arasında bazı Bacillus spp.’ye ait izolatlar fungal hastalık etmeni S. sclerotiorum hifleri üzerinde önemli morfolojik değişikliklere neden olmuştur. Antagonist bakterilerin in vitro antagonizm etkinliği, fungus inokulasyonu öncesi ön inkübasyon süresine bağlı olarak artış göstermiştir. Bacillus spp. ait izolatlar yüksek antagonistik özelliklerinden dolayı organik ve sürdürülebilir tarımı teşvik etmek için toprak kökenli hastalıklarla mücadelede kimyasallara alternatif etkili biyo-kontrol ajanlar olarak kullanılabilir.

In this study, in vitro antagonistic potentials of bacterial microbiomes, obtained from earthworm fertiliser, vermicompost, were investigated on inhibitions of mycelial growth of major foliar and soilborne fungal disease agents Sclerotinia sclerotiorum, Macrophomina phaseolina, Botrytis cinerea, Verticilium dahliae. Total of 69 putative bacterial biocontrol agent (BCA) isolates were obtained from commercial vermicompost lots. Among them, 28 bacterial isolates (49.12% of total isolates) were inhibited mycelial growth of S. sclerotiorum by 1.72-75.43%, M. phaseolina by 1.67-65.83%, B. cinerea by 3.44-57.18%, V dahliae by 2.28-58.74%, respectively. Majorities of bacterial isolates were identified as Bacillus spp. Certain isolates of Bacillus spp. have caused noticeable morphological changes on mycelia of S. sclerotiorum. Antagonistic potentials of bacterial isolates were found to increase by pre-incubation time prior the fungal inoculation. Due to high antagonistic properties, efficient isolates of Bacillus spp.  may be used as biocontrol agent against soilborne diseases as an alternative to pesticides to promote organic and sustainable agriculture.

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Birincil Dil tr
Konular Ziraat
Bölüm ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar

Orcid: 0000-0001-5961-0848
Yazar: Emine Mine SOYLU
Kurum: HATAY MUSTAFA KEMAL ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0003-1002-8958
Yazar: Soner SOYLU (Sorumlu Yazar)
Kurum: HATAY MUSTAFA KEMAL ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0001-7320-3376
Yazar: Merve KARA
Kurum: HATAY MUSTAFA KEMAL ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0003-4545-5968
Yazar: Şener KURT
Kurum: HATAY MUSTAFA KEMAL ÜNİVERSİTESİ
Ülke: Turkey


Destekleyen Kurum Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Komisyonu Başkanlığı
Proje Numarası Proje Numarası: BAP-189
Teşekkür Bu çalışma Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Komisyonu Başkanlığı tarafından finansal olarak desteklenmiştir (Proje Numarası: BAP-189).
Tarihler

Başvuru Tarihi : 5 Ağustos 2019
Kabul Tarihi : 7 Kasım 2019
Yayımlanma Tarihi : 28 Şubat 2020

APA SOYLU, E , SOYLU, S , KARA, M , KURT, Ş . (2020). Sebzelerde Sorun Olan Önemli Bitki Fungal Hastalık Etmenlerine Karşı Vermikomposttan İzole Edilen Mikrobiyomların in vitro Antagonistik Etkilerinin Belirlenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (1) , 7-18 . DOI: 10.18016/ksutarimdoga.vi.601936