Yıl 2020, Cilt 23 , Sayı 3, Sayfalar 762 - 771 2020-06-30

Siyah Alaca ve Doğu Anadolu Kırmızısı Irkına Ait Sığırların Mycoplasma bovis ile Enfekte Sütlerinden Köken Alan Eksozomlardaki Yangı Ile Ilişkili miRNA’ların Ekpresyon Profili
Expression Profiles of Inflammation-related MicroRNAs in Mycoplasma bovis Infected Milk of Holstein-Friesian and Doğu Anadolu Kırmızısı Cows

Selçuk ÖZDEMİR [1]


Mikoplazma bovis, sığırlarda mastitis, artrit ve pnömoni gibi çeşitli klinik hastalıklarla ilişkili önemli bir ajandır. Bu çalışmada, Türkiye'de yetiştirilen Siyah Alaca (SA)  ve Doğu Anadolu Kırmızısı (DAK) sığırlarına ait Mycoplasma bovis ile enfekte subklinik mastitli ve normal sütlerde inflamasyon ile ilişkili miRNA adaylarının belirlenmesi amaçlandı. Mastitli ve normal sığırlardan elde edilen sütteki miRNA'ların ekspresyon seviyeleri qRT-PCR ile analiz edildi. MiR-21, miR-146a, miR-155, miR-222, miR-383, miR-200a, miR-205, miR-122, miR-182'nin ekspresyon düzeylerinin her iki sığıra ait mastitli sütte arttığı gözlendi. Bununla birlikte, miR-21 ve miR-222'nin Holştayn sığırının mastitli sütünde önemli ölçüde arttığı, miR-146a ve miR-383'ün ise DAK sığırının mastitli sütünde önemli ölçüde arttığı belirlendi. Sonuç olarak, subklinik mastitli sütte ekspresyon düzeyi artan miRNA adayları Holştayn ve DAK sığırlarında belirlendi. Araştırmadan elde edilen bulgular, subklinik mastitis sütünde ekspresyon düzeyi artan miRNA'ların Mycoplasma bovis'in neden olduğu subklinik masitit tanısında biyobelirteç olarak kullanılabileceğini göstermiştir.

Mycoplasma bovis is an important pathogen associated with several clinical diseases in cattle, such as mastitis, arthritis, and pneumonia. TableIn this study, we aimed to identify miRNA candidate biomarkers associated with inflammation in Mycoplasma bovis -infected milk samples and normal milk samples of Holstein-Friesian (HF) and Doğu Anadolu Kırmızısı (DAK) cows in Turkey. The expression levels of miRNAs in milk from mastitis-infected cows and uninfected cows were analyzed using a qRT-PCR. The results revealed that miR-21, miR-146a, miR-155, miR-222, miR-383, miR-200a, miR-205, miR-122, and miR-182 were upregulated in mastitis milk. Among the miRNA candidate biomarkers, miR-21 and miR-222 were significantly upregulated only in mastitis milk samples from HF cows, and miR-146a and miR-383 were significantly upregulated only in mastitis milk samples from DAK cows. These results shed light on miRNA candidate biomarkers in milk from HF and DAK cows with subclinical mastitis. The upregulated miRNAs detected in the present study could be used as biomarkers in the diagnosis of subclinical mastitis caused by Mycoplasma bovis.

  • Al-Farha AA, Hemmatzadeh F, Khazandi M, Hoare A, Petrovski K 2017. Evaluation of effects of Mycoplasma mastitis on milk composition in dairy cattle from South Australia. BMC veterinary research 13 (1):351.
  • Al-Farha AA, Khazandi M, Hemmatzadeh F, Jozani R, Tearle R, Hoare A, Petrovski K 2018. Evaluation of three cryoprotectants used with bovine milk affected with Mycoplasma bovis in different freezing conditions. BMC research notes 11 (1):216.
  • Appelt S, Aly SS, Tonooka K, Glenn K, Xue Z, Lehenbauer TW, Marco ML 2019. Development and comparison of loop-mediated isothermal amplification and quantitative polymerase chain reaction assays for the detection of Mycoplasma bovis in milk. Journal of dairy science 102 (3):1985-1996.
  • Behera S, Rana R, Gupta PK, Kumar D, Sonal, Rekha V, Arun TR, Jena D 2018. Development of real-time PCR assay for the detection of Mycoplasma bovis. Tropical animal health and production 50 (4):875-882.
  • Berezikov E 2011. Evolution of microRNA diversity and regulation in animals. Nature reviews Genetics 12 (12):846-860.
  • Cai HY, Bell-Rogers P, Parker L, Prescott JF 2005. Development of a real-time PCR for detection of Mycoplasma bovis in bovine milk and lung samples. Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 17 (6):537-545.
  • Deb R, Kumar A, Chakraborty S, Verma AK, Tiwari R, Dhama K, Singh U, Kumar S 2013. Trends in diagnosis and control of bovine mastitis: a review. Pakistan journal of biological sciences 16 (23):1653-1661.
  • Fox LK 2012 Mycoplasma mastitis: causes, transmission, and control. The Veterinary clinics of North America Food animal practice 28 (2):225-237. Gomes F, Henriques M 2016. Control of Bovine Mastitis: Old and Recent Therapeutic Approaches. Current microbiology 72 (4):377-382. Gu Y, Li M, Wang T, Liang Y, Zhong Z, Wang X, Zhou Q, Chen L, Lang Q, He Z, Chen X, Gong J, Gao X, Li X, Lv X 2012. Lactation-related microRNA expression profiles of porcine breast milk exosomes. PloS one 7 (8):e43691.
  • Gussmann M, Steeneveld W, Kirkeby C, Hogeveen H, Farre M, Halasa T 2019a. Economic and epidemiological impact of different intervention strategies for subclinical and clinical mastitis. Preventive veterinary medicine 166:78-85. doi:10.1016/j.prevetmed.2019.03.001
  • Gussmann M, Steeneveld W, Kirkeby C, Hogeveen H, Nielen M, Farre M, Halasa T 2019b. Economic and epidemiological impact of different intervention strategies for clinical contagious mastitis. Journal of dairy science 102 (2):1483-1493. doi:10.3168/jds.2018-14939
  • Halasa T, Huijps K, Osteras O, Hogeveen H 2007. Economic effects of bovine mastitis and mastitis management: a review. The Veterinary quarterly 29 (1):18-31.
  • Hughes K, Watson CJ 2018. The Mammary Microenvironment in Mastitis in Humans, Dairy Ruminants, Rabbits and Rodents: A One Health Focus. Journal of mammary gland biology and neoplasia 23 (1-2):27-41.
  • Issaq HJ, Blonder J 2009. Electrophoresis and liquid chromatography/tandem mass spectrometry in disease biomarker discovery. Journal of chromatography B, Analytical technologies in the biomedical and life sciences 877 (13):1222-1228.
  • Jiang Q, Zhao H, Li R, Zhang Y, Liu Y, Wang J, Wang X, Ju Z, Liu W, Hou M, Huang J 2019. In silico genome-wide miRNA-QTL-SNPs analyses identify a functional SNP associated with mastitis in Holsteins. BMC genetics 20 (1):46.
  • Jin W, Ibeagha-Awemu EM, Liang G, Beaudoin F, Zhao X, Guan le L 2014. Transcriptome microRNA profiling of bovine mammary epithelial cells challenged with Escherichia coli or Staphylococcus aureus bacteria reveals pathogen directed microRNA expression profiles. BMC genomics 15:181.
  • Josi C, Burki S, Stojiljkovic A, Wellnitz O, Stoffel MH, Pilo P 2018. Bovine Epithelial in vitro Infection Models for Mycoplasma bovis. Frontiers in cellular and infection microbiology 8:329.
  • Lai YC, Fujikawa T, Maemura T, Ando T, Kitahara G, Endo Y, Yamato O, Koiwa M, Kubota C, Miura N 2017. Inflammation-related microRNA expression level in the bovine milk is affected by mastitis. PloS one 12 (5):e0177182.
  • Larrea E, Sole C, Manterola L, Goicoechea I, Armesto M, Arestin M, Caffarel MM, Araujo AM, Araiz M, Fernandez-Mercado M, Lawrie CH 2016. New Concepts in Cancer Biomarkers: Circulating miRNAs in Liquid Biopsies. International journal of molecular sciences 17 (5).
  • Lawless N, Foroushani AB, McCabe MS, O'Farrelly C, Lynn DJ 2013. Next generation sequencing reveals the expression of a unique miRNA profile in response to a gram-positive bacterial infection. PloS one 8 (3):e57543.
  • Li N, Ma J, Guarnera MA, Fang H, Cai L, Jiang F 2014. Digital PCR quantification of miRNAs in sputum for diagnosis of lung cancer. Journal of cancer research and clinical oncology 140 (1):145-150.
  • Li R, Dudemaine PL, Zhao X, Lei C, Ibeagha-Awemu EM 2016. Comparative Analysis of the miRNome of Bovine Milk Fat, Whey and Cells. PloS one 11 (4):e0154129.
  • Li R, Zhang CL, Liao XX, Chen D, Wang WQ, Zhu YH, Geng XH, Ji DJ, Mao YJ, Gong YC, Yang ZP 2015. Transcriptome microRNA profiling of bovine mammary glands infected with Staphylococcus aureus. International journal of molecular sciences 16 (3):4997-5013.
  • Luoreng ZM, Wang XP, Mei CG, Zan LS 2018. Expression profiling of peripheral blood miRNA using RNAseq technology in dairy cows with Escherichia coli-induced mastitis. Scientific reports 8 (1):12693.
  • Ma J, Li N, Guarnera M, Jiang F 2013. Quantification of Plasma miRNAs by Digital PCR for Cancer Diagnosis. Biomarker insights 8:127-136.
  • Murai K, Higuchi H 2019. Prevalence and risk factors of Mycoplasma bovis infection in dairy farms in northern Japan. Research in veterinary science 123:29-31.
  • Naeem A, Zhong K, Moisa SJ, Drackley JK, Moyes KM, Loor JJ 2012. Bioinformatics analysis of microRNA and putative target genes in bovine mammary tissue infected with Streptococcus uberis. Journal of dairy science 95 (11):6397-6408.
  • Ozdemir S, Comakli S 2018. Investigation of the interaction between bta-miR-222 and the estrogen receptor alpha gene in the bovine ovarium. Reproductive biology 18 (3):259-266.
  • Petrovski KR, Trajcev M, Buneski G 2006. A review of the factors affecting the costs of bovine mastitis. Journal of the South African Veterinary Association 77 (2):52-60
  • Rossetti BC, Frey J, Pilo P 2010. Direct detection of Mycoplasma bovis in milk and tissue samples by real-time PCR. Molecular and cellular probes 24 (5):321-323.
  • Sheedy FJ, O'Neill LA 2008. Adding fuel to fire: microRNAs as a new class of mediators of inflammation. Annals of the rheumatic diseases 67 (3):50-55.
  • Sun J, Aswath K, Schroeder SG, Lippolis JD, Reinhardt TA, Sonstegard TS 2015. MicroRNA expression profiles of bovine milk exosomes in response to Staphylococcus aureus infection. BMC genomics 16:806.
  • Vahanikkila N, Pohjanvirta T, Haapala V, Simojoki H, Soveri T, Browning GF, Pelkonen S, Wawegama NK, Autio T 2019. Characterisation of the course of Mycoplasma bovis infection in naturally infected dairy herds. Veterinary microbiology 231:107-115.
  • Weber JA, Baxter DH, Zhang S, Huang DY, Huang KH, Lee MJ, Galas DJ, Wang K 2010. The microRNA spectrum in 12 body fluids. Clinical chemistry 56 (11):1733-1741.
  • Wisselink HJ, Smid B, Plater J, Ridley A, Andersson AM, Aspan A, Pohjanvirta T, Vahanikkila N, Larsen H, Hogberg J, Colin A, Tardy F 2019. A European interlaboratory trial to evaluate the performance of different PCR methods for Mycoplasma bovis diagnosis. BMC veterinary research 15 (1):86
Birincil Dil en
Konular Biyoloji
Bölüm ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar

Orcid: 0000-0001-7539-0523
Yazar: Selçuk ÖZDEMİR (Sorumlu Yazar)
Kurum: Atatürk University,Erzurum,Turkey
Ülke: Turkey


Destekleyen Kurum This study was supported by the Scientific Research Projects Coordination Unit of Atatürk University
Proje Numarası (Project Code: TCD-2017-6381, Project ID: 6381).
Teşekkür This study was supported by the Scientific Research Projects Coordination Unit of Atatürk University (Project Code: TCD-2017-6381, Project ID: 6381).
Tarihler

Başvuru Tarihi : 19 Aralık 2019
Kabul Tarihi : 6 Şubat 2020
Yayımlanma Tarihi : 30 Haziran 2020

APA ÖZDEMİR, S . (2020). Expression Profiles of Inflammation-related MicroRNAs in Mycoplasma bovis Infected Milk of Holstein-Friesian and Doğu Anadolu Kırmızısı Cows. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (3) , 762-771 . DOI: 10.18016/ksutarimdoga.vi.661708