Phoma tracheiphila’ya Karşı Bazı Fungisitlerin Etkinliğinin Araştırılması

Mukaddes Kayım; Merve Yeşil; Ali Endes

doi:10.18016/ksutarimdoga.vi.948066

Araştırma Makalesi

Investigation of the Effectiveness of Some Fungicides Against Phoma tracheiphila

Yıl 2022, Cilt: 25 Sayı: 5, 1086 - 1097, 31.10.2022

Mukaddes Kayım , Merve Yeşil Ali Endes

https://doi.org/10.18016/ksutarimdoga.vi.948066

Cited By: 1

Öz

In this study, the effects of 14 different concentrations of 11 different fungicides, including one licensed, on the mycelial growth of Phoma tracehiphila's most virulent isolates (Pt-E10) were investigated in in vitro conditions. The 5.0 ppm ≥ of cyprodinil (375 g kg-1) + fludioxonil (250 g kg-1) combination and 100 ppm ≥ of fluxapyroxad (75 g L-1) + difenoconazole (50 g L-1) combination were the most effective fungicides with 100 % effect on radial growth inhibition. The EC50 values of these fungicide combinations were calculated for P. tracehiphila (Pt-E10) as 0.12 and 1.23 ppm, respectively. Fungicides with active substances such as azoxystrobin, dithianon and pyraclostrobin did not show any significant effect in preventing mycelial development of P. tracheiphila. In greenhouse trials, the normal doses of cyprodinil (375 g kg-1) + fludioxonil (250 g kg-1) and fluxapyroxad (75 g L-1) + difenoconazole (50 g L-1) fungicide have not been very effective in preventing disease severity. However, when using two fold application doses of fungicides the disease severity was prevented at 62.6% and 45.3%, respectively, and the licensed copper hydroxide prevented the disease only at the rate of 9.8%.

Anahtar Kelimeler

Fungicide , Lemon , Pathogenicity , Plenodomus tracheiphilus

Proje Numarası

FYL2018/10390

Kaynakça

Abbott WS 1987. A method of computing the effectiveness of an insecticide. Journal of the American Mosquito Control Association, (3)2: 302-303
Anonim 2011. https://www.tarimorman.gov.tr/Konu/ 934/Yasaklanan Bitki Koruma Urunleri Aktif Madde Listesi
Anonim 2020. Türkiye Limon Üretimi, 2020. https://www.tuik.gov.tr/ (Alınma tarihi: 29.05.2021)
Anonim 2014. Scientific opinion on pest categorisation of Plenodomus tracheiphilus (petri) Gruyter, Avskamp & Verkley [syn. Phoma tracheiphila (Petri) L.A. Kantschaveli &Gikashvili]. EFSA Panel on Plant Health (PLH). European Food Safety Authority (EFSA), Parma, Italy, 34 pages
Anonim 2019. Dünya Turunçgil Üretimi, 2019. http://www.fao.org/faostat/en/#data/QC (Alınma Tarihi: 29.05.2021)
Coco V, Grimaldi V, Licciardello G, Cirvilleri G, Grasso S, Catara A 2004. Inhibition of Phoma tracheiphila bu Pseudomonas in citrus seedling. Proceedings of the 8th. International Citrus Congress, Agadir, Morocco: 729-732
de Gruyter J, Woudenberg JHC, Aveskamp MM, Verkley GJM, Groenewald JZ, Crous PW 2013. Redisposition of Phoma-like anamorphs in Pleosporales. Studies in Mycology, 75:1-36
Dinç N, Turan K, Salih H 1981. Akdeniz bölgesi limonlarında görülen Uçkurutan hastalığı [Deuterophoma tracheiphila (Petri) Kane. et Ghik.]’nın savaş yöntemleri üzerinde araştırmalar. Bitki Koruma Bülteni, 21(2): 89-99
Elia 1969. Brevi note preliminari su tentativi di lotta endoterapica del mal secco degli Agrumi. Informatore Fitopatologico 19(8): 403-404
Gimenez-Verdu I, Luisi N 1978. Saggio di attivita di due fungicidi sistemici verso il “mal secco” degli Agrumi, Atti Giornate Fitopatologiche 1978. Catania-acireale Italy: 407-415
Kalai-Grami L, Saidi S, Bachkouel S, Slimene IB, Mnari-Hattab M, Hajlaoui MR, Limam F 2014. Isolation and characterization of putative endophytic bacteria antagonistic to Phoma tracheiphila and Verticillium albo-atrum. Appl. Biochem Biotechnol,174: 365-375.
Kantschaveli LA, Gikashvili KG 1948. Materials for the study of “mal secco” or dying up of lemon trees in SSR. Trudy Nauchno-Issledovatel’s kogo. Instituta Zashchity Rastenii Gruzinskoi SSR, 5:1-43
Klotz LJ 1954. Mal Secco disease of Citrus.; Citrus leaves, January 1954
Migheli Q, Cacciola SO, Balmas V, Pane A, Ezra D, Magnano di San Lio G 2009. Mal secco disease caused by Phoma tracheiphila: a potantial threat to lemon production worldwide. Plant Disease, 93: 852-867
Navarro N, Pina JA, Juarez J, Ballester-Olmos JF, Duran-Villa N, Guerri J, Moreno P, Ortega C, Navarro A, Arregui JM, Cambra M, Zaragoza S 2002. The Spanish varietal certification system. Instituto Valenciano de Investigaciones Agrarias, Valencia, Spain
Nigro F, Ippolito A, Salerno MG 2011. Mal secco disease of citrus: a journey through a century of research. Journal of Plant Pathology, 93(3): 523-560
Özdemir KS 2019. Limon ağaçlarında uçkurutan hastalığı’na (Phoma tracheiphila kanc. & ghik.) karşı mikorizal funguslar ve dayanıklılık teşvik edicilerin etkinliğinin belirlenmesi Çukurova Üniversitesi Fen Bilimleri Enstitüsü Doktora tezi, 90 sy
Petri L 1929. Sulla posizione sistematica del fungo parassita delle piante di limone affette da “mal secco” (in Italian). Bolletino della Stazione di Patologia Vegetale, 9: 393-396
Ploetz RC, Perez-Martinez JM, Evans EA, Inch SA 2011. Towards fungicidal management of laurel wilt of avocado. Plant Disease, 95 (8): 977-982
Salerno M, Cutili G 1981. The management of fungal and bacterial diseases of citrus in Italy. Proceeding of the 4th International Citrus Congress, Nowember 1981 Tokyo, Japan. The ınternaional Society of Citriculture, Vol I, Ed. Matsumoto K 1: 360-362
Salerno M, Somma V 1971. Observatione on the systemic nature of benomyl in sour orange seedlings and results of trials against citrus Mal secco. Phytopth. Mediterranea, 10 (1): 99-106 (Rev. App. Pathology 50 (12): 674
Solel Z, Pinkas Y, Shabi E 1977. Internal therapy of mal secco of lemon by pressure infection of fungicides. Netherland J. Plant Pathology, 83 (1): 383-391
Solel Z, Spiegel-Roy P 1978. Methodology of selection of lemon clones for tolerans to mal secco (Phoma tracheiphila) Phytoparasitica, (6)3: 129-134
Somma V, Cutuli G, Li Destri Nicosia O, Solerno M 1978. Accumulation e persistenza del benomyl e del metil-tiofanate in organi vegetativi e frutti di piante di limone trattate alla chioma. Atti Giornate Fitopatologiche 1978, Catania Acireale, Italy:51-57
Tusa N, Bosco SF, Nigro F, Ippolito A 2000. Response of cybrids and somatic hybrid of lemon to Phoma tracheiphila infections. HortScience, (35)1: 125-127
Townsend GR. Heuberger JW 1943. Methods for estimating losses caused by diseases in fungicide experiments. The Plant Disease Reporter, 27: 340–343
Twizeyimana T, McDonald V, Mayarquin JS, Wang DH, Na F, Akgül DS, Eskalen A 2013. Effect of fungicide application on the management of avocado branch canker (formerly Dothiorella canker) in Califonia. Plant Disease, 97 (7): 897-902.

Phoma tracheiphila’ya Karşı Bazı Fungisitlerin Etkinliğinin Araştırılması

Yıl 2022, Cilt: 25 Sayı: 5, 1086 - 1097, 31.10.2022

Mukaddes Kayım , Merve Yeşil Ali Endes

https://doi.org/10.18016/ksutarimdoga.vi.948066

Cited By: 1

Öz

Bu çalışmada bir ruhsatlı olmak üzere 11 farklı fungisitin 14 farklı konsantrasyonunun in vitro koşullarda Phoma tracehiphila’nın en virulent izolatlatının (Pt-E10) miselyal gelişimi üzerine etkileri araştırılmıştır. In vitro koşullarda patojenin miselyal gelişimini engellemede cyprodinil (375 g kg-1)+ fludioxonil (250 g kg-1) kombinasyonunun 5 ppm≥ , fluxapyroxad (75 g l-1) + difenoconazole (50 g l-1) fungisit kombinasyonunun 100 ppm≥ uygulama dozları %100 etki ederek en etkili fungisitler olmuştur. cyprodinil (375 g kg-1)+ fludioxonil (250 g kg-1) ve fluxapyroxad (75 g L-1) + difenoconazole (50 g L-1) Bu fungisit kombinasyonlarının EC50 değerleri sırasıyla 0.12 ve 1.23 ppm olarak P. tracehiphila (Pt-E10) için hesaplanmıştır. Azoxystrobin, dithianon ve pyraclostrobin gibi etkili maddeli fungisitler P. tracheiphila’nın miselyal gelişimini engellemede kayda değer bir etki göstermemiştir. Sera denemelerinde ise cyprodinil (375 g kg-1) + fludioxonil (250 g kg-1) ve fluxapyroxad (75 g L-1) + difenoconazole (50 g L-1) fungisit kombinasyonlarının normal dozları hastalık şiddetini engellemede çok etkili değilken, uygulama dozunun iki katı kullanıldığında hastalık sırasıyla %62.6 ile %45.3 düzeyinde engellenmiş, ruhsatlı bakırhidroksit ise hastalığı sadece % 9.8 oranında engellemiştir.

Anahtar Kelimeler

Fungisit , Limon , Patojenisite , Plenodomus tracheiphilus.

Destekleyen Kurum

Çukurova Üniversitesi, Bilimsel Araştırma Projeleri Birimi

Proje Numarası

FYL2018/10390

Kaynakça

Abbott WS 1987. A method of computing the effectiveness of an insecticide. Journal of the American Mosquito Control Association, (3)2: 302-303
Anonim 2011. https://www.tarimorman.gov.tr/Konu/ 934/Yasaklanan Bitki Koruma Urunleri Aktif Madde Listesi
Anonim 2020. Türkiye Limon Üretimi, 2020. https://www.tuik.gov.tr/ (Alınma tarihi: 29.05.2021)
Anonim 2014. Scientific opinion on pest categorisation of Plenodomus tracheiphilus (petri) Gruyter, Avskamp & Verkley [syn. Phoma tracheiphila (Petri) L.A. Kantschaveli &Gikashvili]. EFSA Panel on Plant Health (PLH). European Food Safety Authority (EFSA), Parma, Italy, 34 pages
Anonim 2019. Dünya Turunçgil Üretimi, 2019. http://www.fao.org/faostat/en/#data/QC (Alınma Tarihi: 29.05.2021)
Coco V, Grimaldi V, Licciardello G, Cirvilleri G, Grasso S, Catara A 2004. Inhibition of Phoma tracheiphila bu Pseudomonas in citrus seedling. Proceedings of the 8th. International Citrus Congress, Agadir, Morocco: 729-732
de Gruyter J, Woudenberg JHC, Aveskamp MM, Verkley GJM, Groenewald JZ, Crous PW 2013. Redisposition of Phoma-like anamorphs in Pleosporales. Studies in Mycology, 75:1-36
Dinç N, Turan K, Salih H 1981. Akdeniz bölgesi limonlarında görülen Uçkurutan hastalığı [Deuterophoma tracheiphila (Petri) Kane. et Ghik.]’nın savaş yöntemleri üzerinde araştırmalar. Bitki Koruma Bülteni, 21(2): 89-99
Elia 1969. Brevi note preliminari su tentativi di lotta endoterapica del mal secco degli Agrumi. Informatore Fitopatologico 19(8): 403-404
Gimenez-Verdu I, Luisi N 1978. Saggio di attivita di due fungicidi sistemici verso il “mal secco” degli Agrumi, Atti Giornate Fitopatologiche 1978. Catania-acireale Italy: 407-415
Kalai-Grami L, Saidi S, Bachkouel S, Slimene IB, Mnari-Hattab M, Hajlaoui MR, Limam F 2014. Isolation and characterization of putative endophytic bacteria antagonistic to Phoma tracheiphila and Verticillium albo-atrum. Appl. Biochem Biotechnol,174: 365-375.
Kantschaveli LA, Gikashvili KG 1948. Materials for the study of “mal secco” or dying up of lemon trees in SSR. Trudy Nauchno-Issledovatel’s kogo. Instituta Zashchity Rastenii Gruzinskoi SSR, 5:1-43
Klotz LJ 1954. Mal Secco disease of Citrus.; Citrus leaves, January 1954
Migheli Q, Cacciola SO, Balmas V, Pane A, Ezra D, Magnano di San Lio G 2009. Mal secco disease caused by Phoma tracheiphila: a potantial threat to lemon production worldwide. Plant Disease, 93: 852-867
Navarro N, Pina JA, Juarez J, Ballester-Olmos JF, Duran-Villa N, Guerri J, Moreno P, Ortega C, Navarro A, Arregui JM, Cambra M, Zaragoza S 2002. The Spanish varietal certification system. Instituto Valenciano de Investigaciones Agrarias, Valencia, Spain
Nigro F, Ippolito A, Salerno MG 2011. Mal secco disease of citrus: a journey through a century of research. Journal of Plant Pathology, 93(3): 523-560
Özdemir KS 2019. Limon ağaçlarında uçkurutan hastalığı’na (Phoma tracheiphila kanc. & ghik.) karşı mikorizal funguslar ve dayanıklılık teşvik edicilerin etkinliğinin belirlenmesi Çukurova Üniversitesi Fen Bilimleri Enstitüsü Doktora tezi, 90 sy
Petri L 1929. Sulla posizione sistematica del fungo parassita delle piante di limone affette da “mal secco” (in Italian). Bolletino della Stazione di Patologia Vegetale, 9: 393-396
Ploetz RC, Perez-Martinez JM, Evans EA, Inch SA 2011. Towards fungicidal management of laurel wilt of avocado. Plant Disease, 95 (8): 977-982
Salerno M, Cutili G 1981. The management of fungal and bacterial diseases of citrus in Italy. Proceeding of the 4th International Citrus Congress, Nowember 1981 Tokyo, Japan. The ınternaional Society of Citriculture, Vol I, Ed. Matsumoto K 1: 360-362
Salerno M, Somma V 1971. Observatione on the systemic nature of benomyl in sour orange seedlings and results of trials against citrus Mal secco. Phytopth. Mediterranea, 10 (1): 99-106 (Rev. App. Pathology 50 (12): 674
Solel Z, Pinkas Y, Shabi E 1977. Internal therapy of mal secco of lemon by pressure infection of fungicides. Netherland J. Plant Pathology, 83 (1): 383-391
Solel Z, Spiegel-Roy P 1978. Methodology of selection of lemon clones for tolerans to mal secco (Phoma tracheiphila) Phytoparasitica, (6)3: 129-134
Somma V, Cutuli G, Li Destri Nicosia O, Solerno M 1978. Accumulation e persistenza del benomyl e del metil-tiofanate in organi vegetativi e frutti di piante di limone trattate alla chioma. Atti Giornate Fitopatologiche 1978, Catania Acireale, Italy:51-57
Tusa N, Bosco SF, Nigro F, Ippolito A 2000. Response of cybrids and somatic hybrid of lemon to Phoma tracheiphila infections. HortScience, (35)1: 125-127
Townsend GR. Heuberger JW 1943. Methods for estimating losses caused by diseases in fungicide experiments. The Plant Disease Reporter, 27: 340–343
Twizeyimana T, McDonald V, Mayarquin JS, Wang DH, Na F, Akgül DS, Eskalen A 2013. Effect of fungicide application on the management of avocado branch canker (formerly Dothiorella canker) in Califonia. Plant Disease, 97 (7): 897-902.

Toplam 27 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	Türkçe
Konular	Ziraat, Veterinerlik ve Gıda Bilimleri
Bölüm	ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar	Mukaddes Kayım 0000-0003-0309-0390 Merve Yeşil 0000-0003-4983-2871 Ali Endes 0000-0003-4815-5864
Proje Numarası	FYL2018/10390
Yayımlanma Tarihi	31 Ekim 2022
Gönderilme Tarihi	5 Haziran 2021
Kabul Tarihi	11 Ekim 2021
Yayımlandığı Sayı	Yıl 2022 Cilt: 25 Sayı: 5

Kaynak Göster

APA	Kayım, M., Yeşil, M., & Endes, A. (2022). Phoma tracheiphila’ya Karşı Bazı Fungisitlerin Etkinliğinin Araştırılması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(5), 1086-1097. https://doi.org/10.18016/ksutarimdoga.vi.948066