Determination of metabolic profiling by LC-MS/MS, evaluation of antioxidant activities, and enzyme inhibition effects of Helichrysum plicatum subsp. pseudopliacatum

Leyla Güven; İlhami Gülçin

doi:10.18016/ksutarimdoga.vi.1360450

Araştırma Makalesi

Determination of metabolic profiling by LC-MS/MS, evaluation of antioxidant activities, and enzyme inhibition effects of Helichrysum plicatum subsp. pseudopliacatum

Yıl 2024, Cilt: 27 Sayı: 3, 501 - 514, 30.06.2024

Leyla Güven , İlhami Gülçin

https://doi.org/10.18016/ksutarimdoga.vi.1360450

Cited By: 2

Öz

Helichrysum species are traditionally used to treat many diseases. In this study, the antioxidant and enzyme inhibition abilities of methanol (MEHP) and water extracts (WEHP) from Helichrysum plicatum subsp. Pseudopliacatum was evaluated. Also, the phenolic compounds will be assessed and the quantities of total phenols and flavonoids will be calculated. For evaluation of antioxidant activity of both extracts was assessed using DPPH, ABTS and DMPD radical scavenging, FRAP, CUPRAC, and Fe3+ reduction methods were used. The inhibition effects of extracts were spectrophotometrically evaluated on the α-glucosidase (α-Gly), acetylcholinesterase (AChE), and carbonic anhydrase II (CA II) enzymes. These inhibition effects were compared to the standard inhibitors The qualitative and quantitative phenolic components in extracts were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS), employing both methods. The IC50 value, which is the inhibitor concentration that causes 50% inhibition of MEHP and WEHP, is found as 2.39 and 2.97 μg mL-1 for α-gly, 2.48 and 3.59 μg mL-1 for AChE and 11.95 and 25.67 μg mL-1for CA II. The quantity of phenols in MEHP and WEHP were 46.00 and 33.50 μg GAE mg-1 extract, 62.13 and 103.93 μg QE mg-1 extract flavonoids, respectively. IC50 values of MEHP and WEHP were calculated as 40.77 and 49.51 μg mL-1 for DPPH radical scavenging, 10.50 and 17.77 μg mL-1 ABTS radical scavenging and 99.02 and 77.01 μg mL-1 DMPD radical scavenging. The absorbance values of reducing abilities of MEHP and WEHP were found as 0.555 and 0.495 Fe3+ reducing ability, 0.577 and 0.286 for FRAP reducing ability and 0.402 and 0.315 for Cu2+ reducing ability. As a result of the LC-MS/MS analysis, the levels of phenolic compounds such as chlorogenic acid, cyanidin-3-O-glucoside and quinic acid were found to be high in MEHP and WEHP. In this study, it was observed that MEHP and WEHP have strong antioxidant properties and effectively inhibited the enzymes activities. The findings clearly reveal the antioxidant and enzyme inhibition potential of Helichrysum species, which have been used in traditional medicine for a long time. Helichrysum plicatum extracts used in this study show that they are very effective against diabetes, glaucoma and Alzheimer's disease, which are global and common diseases.

Anahtar Kelimeler

Antioxidant , Enzyme inhibition , LC-MS/MS , Helichrysum , Asteraceae

Kaynakça

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Al-Rehaily, A. J., Albishi, O. A., El-Olemy, M. M., & Mossa, J. S. (2008). Flavonoids and terpenoids from Helichrysum forskahlii. Phytochemistry, 69 (9), 1910-1914.
Albayrak, S., Aksoy, A., Sagdic, O., & Hamzaoglu, E. (2010a). Compositions, antioxidant and antimicrobial activities of Helichrysum (Asteraceae) species collected from Turkey. Food Chemistry, 119 (1), 114-122.
Albayrak, S., Aksoy, A., Sağdıç, O., & Budak, Ü. (2010b). Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. Turkish Journal of Biology, 34 (4), 463-473.
Apak, R., Calokerinos, A., Gorinstein, S., Segundo, M.A., Hibbert, D.B., Gülçin, İ., Demirci Çekiç, S., Güçlü, K., Özyürek, M., Esin Çelik, S., Magalhaes, L.M., & Arancibia-Avila P. (2022). Methods to evaluate the scavenging activity of antioxidants toward reactive oxygen and nitrogen species. Pure and Applied Chemistry, 94(1), 87-144.
Aslan, M., Deliorman, O. D., Orhan, N., Sezik, E., & Yesilada, E. (2007). In vivo antidiabetic and antioxidant potential of Helichrysum plicatum ssp. plicatum capitulums in streptozotocin-induced-diabetic rats. Journal of Ethnopharmacology, 109 (1), 54-59.
Aydin, T. (2020). Secondary metabolites of Helichrysum plicatum DC. subsp. plicatum flowers as strong carbonic anhydrase, cholinesterase and alpha-glycosidase inhibitors. Zeitschrift Fur Naturforschung Section C- Journal of Biosciences, 75 (5-6), 153-159.
Bayir, Y., Halici, Z., Keles, M. S., Colak, S., Cakir, A., Kaya, Y., & Akcay, F. (2011). Helichrysum plicatum DC. subsp. plicatum extract as a preventive agent in experimentally induced urolithiasis model. Journal of Ethnopharmacology, 138 (2), 408-414.
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Benelli, G., Pavela, R., Rakotosaona, R., Randrianarivo, E., Nicoletti, M., & Maggi, F. (2018). Chemical composition and insecticidal activity of the essential oil from Helichrysum faradifani endemic to Madagascar. Natural Product Research, 32 (14), 1690-1698.
Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181 (4617), 1199-1200.
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Carini, M., Aldini, G., Furlanetto, S., Stefani, R., & Facino, R. M. (2001). LC coupled to ion-trap MS for the rapid screening and detection of polyphenol antioxidants from Helichrysum stoechas. Journal of Pharmaceutical and Biomedical Analysis, 24 (3), 517-526.
Çetinkaya, Y., Göçer, H., Menzek, A., & Gülçin, İ. (2012). Synthesis and antioxidant properties of (3,4-dihydroxyphenyl)(2,3,4-trihydroxyphenyl)methanone and its derivatives. Archiv der Pharmazie, 345 (4), 323-334.
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Helichrysum plicatum subsp. pseudopliacatum'un LC-MS/MS ile metabolik profilinin belirlenmesi, antioksidan aktivitelerinin ve enzim inhibisyon etkilerinin değerlendirilmesi

Yıl 2024, Cilt: 27 Sayı: 3, 501 - 514, 30.06.2024

Leyla Güven , İlhami Gülçin

https://doi.org/10.18016/ksutarimdoga.vi.1360450

Cited By: 2

Öz

Helichrysum türleri geleneksel olarak birçok hastalık tedavisinde kullanılmaktadır. Bu çalışmada, Helichrysum plicatum subsp. pseudopliacatum’den elde edilen metanol (MEHP) ve su (WEHP) ekstrelerinin antioksidan ve enzim inhibisyon etkilerinin belirlenmesi amaçlanmıştır. Ayrıca her iki ekstrede bulunan fenolik bileşiklerin belirlenmesi ve toplam fenolik ve flavonoit miktarlarının hesaplanması da amaçlanmıştır. Her iki ekstrenin antioksidan aktivitesini değerlendirmek için DPPH, ABTS ve DMPD radikal giderme, FRAP, Cu2+ ve Fe3+ indirgeme metotları kullanılmıştır. Ekstrelerin α-glikozidaz (α-Gly), asetilkolinesteraz (AChE) ve karbonik anhidraz II (CA II) enzimleri üzerinde inhibisyon etkileri spektrofotometrik olarak değerlendirildi ve standart inhibitörler ile mukayese edildi. Ekstrelerde bulunan fenolik maddelerin kalitatif ve kantitatif analizi ise sıvı kromatografisi tandem kütle spektrometresi (LC-MS/MS) ile belirlendi.
MEHP ve WEHP’ye ait %50 inhibisyona sebep olan inhibitör konsantrasyonu olan IC50 değeri, α-Gly için 2.39 ve 2.97 μg mL-1, AChE için 2.48 ve 3.59 μg mL-1 ve CA II için ise 11.95 ve 25.67 μg mL-1 olarak bulundu. MEHP ve WEHP'deki toplam fenolik miktarı sırasıyla 46.00 ve 33.50 μg GAE mg-1 ekstre; toplam flavonoit ise 62.13 ve 103.93 μg QE mg-1 ekstre olarak hesaplandı. MEHP ve WEHP’nin sırasıyla DPPH radikal gidermeye ait IC50 değerleri 40.77 ve 49.51 µg/mL, ABTS radikal gidermeye ait IC50 değerleri ise 10.50 ve 17.77 µg mL-1 ve DMPD radikal gidermeye ait IC50 değerleri IC50= 99.02 ve 77.01 µg mL-1 olarak belirlenmiştir. MEHP ve WEHP’in Fe3+ indirgeme kapasitesine ait absorbans değerleri sırasıyla indirgeme 0.555 ve 0.495, FRAP indirgeme kapasitesine ait absorbans değerleri 0.577 ve 0.286 ve Cu2+ indirgeme kapasitesine ait absorbans değerleri ise 0.402 ve 0.315 olarak bulundu. LC-MS/MS analizi sonucunda MEHP ve WEHP'de klorojenik asit, siyanidin-3-O-glikozit ve kinik asidin en fazla bulunduğu belirlendi. Bu çalışmada, MEHP ve WEHP'in etkili antioksidan etkiye sahip olduğunu ve α-Gly, AChE ve CA II enzimlerini etkili bir şekilde inhibe ettiği gözlendi. Elde edilen bulgular, geleneksel tıpta uzun süredir kullanılan Helichrysum türlerinin antioksidan ve enzim inhibisyon potansiyelini açık bir şekilde ortaya koymaktadır. Bu çalışmada kullanılan Helichrysum plicatum ekstreleri global ve yaygın birer hastalık olan diyabet, glokom ve Alzheimer hastalığına karşı oldukça etkili olduklarını gösterdi.

Anahtar Kelimeler

Antioksidan , Enzim inhibisyon , LC-MS/MS , Helichrysum , Asteraceae

Kaynakça

Ak, T., & Gülçin, İ. (2008). Antioxidant and radical scavenging properties of curcumin. Chemico-Biological Interactions, 174 (1), 27-37.
Al-Rehaily, A. J., Albishi, O. A., El-Olemy, M. M., & Mossa, J. S. (2008). Flavonoids and terpenoids from Helichrysum forskahlii. Phytochemistry, 69 (9), 1910-1914.
Albayrak, S., Aksoy, A., Sagdic, O., & Hamzaoglu, E. (2010a). Compositions, antioxidant and antimicrobial activities of Helichrysum (Asteraceae) species collected from Turkey. Food Chemistry, 119 (1), 114-122.
Albayrak, S., Aksoy, A., Sağdıç, O., & Budak, Ü. (2010b). Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. Turkish Journal of Biology, 34 (4), 463-473.
Apak, R., Calokerinos, A., Gorinstein, S., Segundo, M.A., Hibbert, D.B., Gülçin, İ., Demirci Çekiç, S., Güçlü, K., Özyürek, M., Esin Çelik, S., Magalhaes, L.M., & Arancibia-Avila P. (2022). Methods to evaluate the scavenging activity of antioxidants toward reactive oxygen and nitrogen species. Pure and Applied Chemistry, 94(1), 87-144.
Aslan, M., Deliorman, O. D., Orhan, N., Sezik, E., & Yesilada, E. (2007). In vivo antidiabetic and antioxidant potential of Helichrysum plicatum ssp. plicatum capitulums in streptozotocin-induced-diabetic rats. Journal of Ethnopharmacology, 109 (1), 54-59.
Aydin, T. (2020). Secondary metabolites of Helichrysum plicatum DC. subsp. plicatum flowers as strong carbonic anhydrase, cholinesterase and alpha-glycosidase inhibitors. Zeitschrift Fur Naturforschung Section C- Journal of Biosciences, 75 (5-6), 153-159.
Bayir, Y., Halici, Z., Keles, M. S., Colak, S., Cakir, A., Kaya, Y., & Akcay, F. (2011). Helichrysum plicatum DC. subsp. plicatum extract as a preventive agent in experimentally induced urolithiasis model. Journal of Ethnopharmacology, 138 (2), 408-414.
Bayrak, C., Taslimi, P., Karaman, H. S., Gulcin, I., & Menzek, A. (2019). The first synthesis, carbonic anhydrase inhibition and anticholinergic activities of some bromophenol derivatives with S including natural products. Bioorganic Chemistry, 85, 128-139.
Benelli, G., Pavela, R., Rakotosaona, R., Randrianarivo, E., Nicoletti, M., & Maggi, F. (2018). Chemical composition and insecticidal activity of the essential oil from Helichrysum faradifani endemic to Madagascar. Natural Product Research, 32 (14), 1690-1698.
Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181 (4617), 1199-1200.
Bursal, E., & Köksal, E. (2011). Evaluation of reducing power and radical scavenging activities of water and ethanol extracts from sumac (Rhus coriaria L.). Food Research International, 44 (7), 2217-2221.
Bursal, E., Köksal, E., Gülçin, İ., Bilsel, G., & Gören, A. C. (2013). Antioxidant activity and polyphenol content of cherry stem (Cerasus avium L.) determined by LC–MS/MS. Food Research International, 51(1), 66-74.
Carini, M., Aldini, G., Furlanetto, S., Stefani, R., & Facino, R. M. (2001). LC coupled to ion-trap MS for the rapid screening and detection of polyphenol antioxidants from Helichrysum stoechas. Journal of Pharmaceutical and Biomedical Analysis, 24 (3), 517-526.
Çetinkaya, Y., Göçer, H., Menzek, A., & Gülçin, İ. (2012). Synthesis and antioxidant properties of (3,4-dihydroxyphenyl)(2,3,4-trihydroxyphenyl)methanone and its derivatives. Archiv der Pharmazie, 345 (4), 323-334.
Davis, P., & Kupicha, K. (1975). Helichrysum. In P. H. Davis (Ed.), Flora of Turkey and the East Aegean Islands (Vol. 5, pp. 80-96). the University Press.
Durmaz, L., Kiziltas, H., Guven, L., Karagecili, H., Alwasel, S., & Gulcin, İ. (2022). Antioxidant, antidiabetic, anticholinergic, and antiglaucoma effects of magnofluorine. Molecules, 27 (18), 5902.
Ellman, G. L., Courtney, K. D., Andres, V., & Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7 (2), 88-95.
Farris, E., Canopoli, L., Cucca, E., Landi, S., Maccioni, A., & Filigheddu, R. (2017). Foxes provide a direct dispersal service to Phoenician junipers in Mediterranean coastal environments: ecological and evolutionary implications. Plant Ecology and Evolution, 150 (2), 117-128.
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Toplam 61 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	İngilizce
Konular	Farmasotik Botanik
Bölüm	ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar	Leyla Güven 0000-0002-3189-6415 İlhami Gülçin 0000-0001-5993-1668
Erken Görünüm Tarihi	11 Mart 2024
Yayımlanma Tarihi	30 Haziran 2024
Gönderilme Tarihi	15 Eylül 2023
Kabul Tarihi	2 Kasım 2023
Yayımlandığı Sayı	Yıl 2024 Cilt: 27 Sayı: 3

Kaynak Göster

APA	Güven, L., & Gülçin, İ. (2024). Determination of metabolic profiling by LC-MS/MS, evaluation of antioxidant activities, and enzyme inhibition effects of Helichrysum plicatum subsp. pseudopliacatum. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 27(3), 501-514. https://doi.org/10.18016/ksutarimdoga.vi.1360450