Determination of Antimicrobial Activity of Nasturtium officinale and Its Content of Volatile Organic Compounds and Fatty Acids

Leyla Ercan; Mehmet Doğru

doi:10.18016/ksutarimdoga.vi.1001837

EN TR

Determination of Antimicrobial Activity of Nasturtium officinale and Its Content of Volatile Organic Compounds and Fatty Acids

Abstract

Due to the side effects of antibiotics used in the treatment of diseases caused by pathogenic bacteria, and antibiotic resistance that develops due to the misuse of antibiotics, scientists have turned to the search for alternative antimicrobial compounds. Plants and antimicrobial compounds in plants are widely researched because they are natural and have been a familiar resource in the field of complementary medicine for centuries. In this study, antimicrobial activities of the methanol and water extracts of Nasturtium officinale prepared at different concentrations were investigated on gram-positive bacteria, gram-negative bacteria, and fungi by the disc diffusion method. In addition, volatile organic compound and fatty acidcontent of the plant were determined. For this purpose, fatty acids were determined by converting them to methyl esters in GC-FID (gas chromatography flame ionization detector), volatile compounds were determined by SPME (Solid-phase microextraction) method in GC-MS (gas chromatography-mass spectrometry). In addition, the amounts of volatile components in different parts of the plant were shown comparatively within the scope of the research. According to the results obtained; it was revealed that Nasturtium officinale has an antimicrobial effect on Bacillus megaterium, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, Bacillus Spizizenii, Klebsiella pneumoniae, Staphylococcus aureus bacteria. The plant showed a stronger antimicrobial effect, especially on P. aeruginosa, C. Albicans, and E. coli. It has also been determined that Nasturtium officinale has important essential fatty acids as well as many volatile components. In the analyzes made, it was determined that the main volatile component of Nasturtium officinale was alpha-Terpinolene.

Keywords

Nasturtium officinale’nin Antimikrobiyal Aktivitesinin ve İçeriğindeki Uçucu Organik Bileşikler ve Yağ Asitlerinin Belirlenmesi

Öz

Patojen bakterilerin neden olduğu hastalıkların tedavisinde kullanılan antibiyotiklerin gerek yan etkileri, gerekse yanlış kullanımına bağlı olarak gelişen antibiyotik direnci nedeniyle bilim insanları alternatif antimikrobiyal bileşik arayışına yönelmiştir. Doğal olması ve yüzyıllardır tamamlayıcı tıp alanında tanıdık bir kaynak olması sebebiyle bitkiler ve bitkilerdeki antimikrobiyal bileşenler yaygın bir şekilde araştırılmaktadır. Bu çalışmada, Nasturtium officinale’nin farklı konsantrasyonlarda hazırlanan metanol ve su ekstraktlarının gram-pozitif bakteriler, gram-negatif bakteriler ve mantar üzerindeki antimikrobiyal aktivitesi disk difüzyon yöntemiyle araştırıldı. Bunun yanısıra bitkinin içeriğindeki uçucu organik bileşikler ve yağ asitleri tespit edildi. Bu amaçla GC-FID'de (gaz kromatografisi alev iyonizasyon dedektörü) yağ asitleri metil esterlere dönüştürülerek belirlenirken, GC-MS'de (gaz kromatografisi - kütle spektrometrisi) SPME (Katı fazlı mikroekstraksiyon) yöntemi ile uçucu bileşenleri belirlendi. Ayrıca araştırma kapsamında bitkinin farklı kısımlarındaki uçucu bileşenler karşılaştırmalı olarak ele alındı. Elde edilen sonuçlara göre; Nasturtium officinale'nin Bacillus megaterium, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, Bacillus Spizizenii, Klebsiella pneumoniae, Staphylococcus aureus bakterileri üzerinde antimikrobiyal etkisi olduğu ortaya çıktı. Bitki özellikle P. aeruginosa, C. Albicans ve E. Coli üzerinde daha güçlü antimikrobiyal etki göstermiştir. Ayrıca Nasturtium officinale'nin önemli esansiyel yağ asitlerinin yanısıra çok sayıda uçucu bileşenlere de sahip olduğu tespit edildi. Yapılan analizlerde Nasturtium officinale’nin ana uçucu bileşeninin alfa-Terpinolen olduğu belirlendi.

Anahtar Kelimeler

Destekleyen Kurum

Dicle University Scientific Research Projects Coordination Unit.(Dicle Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi.)

Proje Numarası

ZGEF.20.001

Teşekkür

We would like to thank Dicle University Science and Technology Application and Research Center (DUBTAM) for the analyzes. (Analizler için Dicle Üniversitesi Bilim ve Teknoloji Uygulama ve Araştırma Merkezi'ne (DUBTAM) teşekkür ederiz.)

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Leyla Ercan ^*
0000-0002-6570-8128
Türkiye

Mehmet Doğru
0000-0002-2287-2913
Türkiye

Yayımlanma Tarihi

30 Aralık 2022

Gönderilme Tarihi

30 Eylül 2021

Kabul Tarihi

14 Ocak 2022

Yayımlandığı Sayı

Yıl 2022 Cilt: 25 Sayı: Ek Sayı 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1001837

IZ

https://izlik.org/JA46YY47PC

Kaynak Göster

RIS / Bibtex

APA

Ercan, L., & Doğru, M. (2022). Determination of Antimicrobial Activity of Nasturtium officinale and Its Content of Volatile Organic Compounds and Fatty Acids. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(Ek Sayı 1), 11-21. https://doi.org/10.18016/ksutarimdoga.vi.1001837