Standardization of Infusion of Lycium barbarum Grown by Organic Farming Methods and Enzyme Inhibitory and Antioxidant Activities

Burçin Özüpek; Sultan Pekacar; Didem Deliorman Orhan

doi:10.18016/ksutarimdoga.vi.1200718

TR EN

Organik Tarım Yöntemleri ile Yetiştirilmiş Lycium barbarum İnfüzyonunun Standardizasyonu ve Antioksidan ve Enzim İnhibitör Etkileri

Öz

Tükenen doğal kaynaklar sebebiyle biyoçeşitliliği korumak maksatlı tıbbi ve aromatik bitkilerin konvansiyonel ve organik tarım teknikleri ile yetiştirilmesi giderek yaygınlaşmaktadır. Organik tarım tekniklerinin pahalı olması yetiştirilmesi düşünülen bitkilerin daha dikkatli seçilmesini gerektir. Bu bitkilerin biyoaktivitelerinin ve fitokimyasal içeriklerinin de değerlendirilmesi ilaç ve gıda sanayi için önem arz etmektedir. Bu çalışmada, organik tarım teknikleri ile yetiştirilen Lycium barbarum’un meyve, kök ve yapraklarından infüzyon ve dekoksiyon teknikleri kullanılarak elde edilen ekstrelerin antioksidan, antidiyabetik, antikolesterolemik ve antiobezite aktiviteleri in vitro olarak değerlendirilmiştir. Ekstrelerin fitokimyasal içerikleri spektroskopik ve kromatografik tekniklerle incelenmiştir. Yaprak infüzyonunun en yüksek toplam fenol (72.53±9.13 mg GAE g-1 ekstre) ve toplam flavonoid (14.92±0.53 mg QE g-1 ekstre) içeriğine sahip olduğu bulunmuştur. Beş farklı antioksidan aktivite yönteminin (ABTS ve DPPH radikal süpürücü aktivite, metal bağlama kapasitesi, toplam antioksidan kapasite ve demir indirgeme gücü) kullanıldığı çalışmada, tüm bulgular değerlendirildiğinde L. barbarum kök dekoksiyonu, toplam antioksidan kapasite yöntemi dışındaki tüm yöntemlerde güçlü bir antioksidan etki göstermiştir. Ekstrelerin hiçbiri α-glukozidaz enzimi üzerinde inhibitör etki göstermezken, 2 mg mL-1 konsantrasyonda bitkinin yaprak infüzyonu referans bileşiklerle (orlistat ve simvastatin) karşılaştırıldığında özellikle pankreatik lipazı ve pankreatik kolesterol esteraz enzimleri üzerinde güçlü inhibisyonlara neden olmuştur. Bu sonuçlar ışığında L. barbarum yaprağı infüzyonu, markör olarak klorojenik asit (1.339±0.056 g 100g-1 kuru ekstre) ve kersetin-3-O-glukozit (1.801±0.042 g 100g-1 kuru ekstre) üzerinden RP-HPLC tekniği ile standardize edilmiştir. Bulgular, organik tarım teknikleriyle yetiştirilen L. barbarum’un yaprak infüzyonlarının, hiperkolesterolemi ve obezite kontrolü için doğal ürün geliştirme çalışmalarının kaynağı olabileceğini ve ekstrenin markör olarak klorojenik asit ve kersetin-3-O-glukozit kullanılarak standardize edilebileceğini göstermiştir.

Anahtar Kelimeler

Standardization of Infusion of Lycium barbarum Grown by Organic Farming Methods and Enzyme Inhibitory and Antioxidant Activities

Abstract

The cultivation of medicinal and aromatic plants with conventional and organic farming techniques in order to protect biodiversity due to depleted natural resources is becoming increasingly common. The expense of organic farming techniques necessitates more careful selection of the plants to be grown. Evaluation of the bioactivity and phytochemical contents of these plants is important for the pharmaceutical and food industry. In this study, the antioxidant, antidiabetic, anticholesterolemic and antiobesity activities of the extracts obtained from the fruit, root and leaves of Lycium barbarum grown with organic farming techniques using infusion and decoction techniques were evaluated in vitro. The phytochemical contents of the extracts were investigated by spectroscopic and chromatographic techniques. In the study in which five different antioxidant activity methods were used, L. barbarum root decoction showed a strong antioxidant effect in almost all methods. While none of the extracts exerted an inhibitory effect on the α-glucosidase enzyme, the leaf infusion of the plant at 2 mg mL-1 concentration caused strong inhibitions especially on pancreatic lipase (62.16±3.33%) and pancreatic cholesterol esterase (93.98±0.54%) enzymes compared to the reference compounds. L. barbarum leaf infusion was standardized by RP-HPLC technique on the basis of chlorogenic acid (1.339±0.056 g 100g-1 dry extract) and quercetin-3-O-glucoside (1.801±0.042 g 100g-1 dry extract) as markers. The findings displayed that leaf infusions of L. barbarum grown with organic farming techniques could be the source of natural product development studies for hypercholesterolemia and obesity control, and the extract could be standardized using chlorogenic acid and quercetin-3-O-glucoside as markers.

Keywords

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Burçin Özüpek
0000-0003-2159-9860
Türkiye

Sultan Pekacar ^*
0000-0002-7782-9832
Türkiye

Didem Deliorman Orhan
0000-0003-3916-4048
Türkiye

Erken Görünüm Tarihi

13 Ekim 2023

Yayımlanma Tarihi

28 Şubat 2024

Gönderilme Tarihi

7 Kasım 2022

Kabul Tarihi

8 Haziran 2023

Yayımlandığı Sayı

Yıl 2024 Cilt: 27 Sayı: 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1200718

IZ

https://izlik.org/JA98YE48TE

Kaynak Göster

RIS / Bibtex

APA

Özüpek, B., Pekacar, S., & Deliorman Orhan, D. (2024). Standardization of Infusion of Lycium barbarum Grown by Organic Farming Methods and Enzyme Inhibitory and Antioxidant Activities. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 27(1), 26-37. https://doi.org/10.18016/ksutarimdoga.vi.1200718

Organik Tarım Yöntemleri ile Yetiştirilmiş Lycium barbarum İnfüzyonunun Standardizasyonu ve Antioksidan ve Enzim İnhibitör Etkileri

Öz

Anahtar Kelimeler

Standardization of Infusion of Lycium barbarum Grown by Organic Farming Methods and Enzyme Inhibitory and Antioxidant Activities

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

e-ISSN: 2619-9149