In vitro Antimicrobial Activity, Antioxidant Activity and Synergistic/Antagonistic Effect of Arum maculatum L. in Mersin Province, Turkey

Mürüvvet Düz

doi:10.18016/ksutarimdoga.vi.1225076

TR EN

Arum maculatum L.'nin in vitro Antimikrobiyal Aktivitesi, Antioksidan Aktivitesi ve Sinerjistik/Antagonistik Etkisi

Öz

Arum maculatum Türkiye'de yerel halk arasında kolit ve iç kanamalı hemoroid tedavisinde kullanılmaktadır. Bu çalışma, Arum maculatum L.'nin yaprak ve kök metanol ekstraktının antioksidan ve antimikrobiyal aktivitesini ve koruyucu etkilerini değerlendirmek için tasarlanmıştır. Kök ve yaprak ekstraktının fenolik bileşik içeriği sırasıyla 207.135±0.07 µg GAE g-1, 386.054±0.7 µg GAE g-1 ekstrakt olarak belirlenmiştir. Toplam flavonoid miktarı sırasıyla 53.386±0.220 µg QE g-1 ve 347.704±0.352 µg QE g-1 ekstrakt olarak belirlenmiştir. Yaprak ve kök ekstraktlarının DPPH serbest radikal süpürmesi sırasıyla %76.69 ve %67.33 olarak belirlendi. Metal şelatlama etkisi kök ve yapraklar için sırasıyla 46.585±0.025 mg EDTA/g ekstrakt ve 35.610±0.087 mg EDTA g-1 ekstrakt olarak tespit edilmiştir. Tüm ekstraktlar, test edilen tüm bakterilere karşı etkili olmuş ve Staphylococcus mutans ATTC 10449, yaprak ve kök ekstraktları için sırasıyla en düşük MİK değeri (0,20 mg ml -1 0,81 mg ml-1) ile en duyarlı bakteri olmuştur. Kloramfenikol ile karşılaştırıldığında en büyük zon inhibisyon bölgesi çapı (8±2,44 mm, 9±3,26) bulunmuştur. Arum yaprak ekstraktı Escherichia coli ve Staphylococcus mutans'a karşı OFX ile sinerjistik bir etki göstermiştir ve Arum kök ekstraktı Staphylococcus aureus ve Staphylococcus mutans'a karşı OFX ile sinerjistik bir etkiye sahiptir. Bu sonuçlar, A. maculatum'un antioksidan ve antibakteriyel özelliklere sahip kayda değer doğal biyoaktif bileşiklere sahip olduğunu göstermiştir.

Anahtar Kelimeler

In vitro Antimicrobial Activity, Antioxidant Activity and Synergistic/Antagonistic Effect of Arum maculatum L. in Mersin Province, Turkey

Abstract

Arum maculatum is used for the treatment of colitis and internal bleeding haemorrhoids among the local people in Turkey. This study was designed to evaluate the antioxidant and antimicrobial activity and protective effects of the leaf and root methanol extract of Arum maculatum L. The phenolic compound content of the root and leaf extract was determined as 207.135±0.07 µg GAE g-1, and 386.054±0.7 µg GAE g extract-1, respectively. The total flavonoid amount was determined as 53.386±0.220 µg QE g-1 and 347.704±0.352 µg QE g-1 extract, respectively. DPPH free radical scavenging of leaf and root extracts was determined as 76.69% and 67.33%, respectively.. The metal chelating effect was determined as 46.585±0.025 mg EDTA/g extract and 35.610±0.087 mg EDTA g extract-1 for roots and leaves, respectively. All extracts were effective against all tested bacteria, and Staphylococcus mutans ATTC 10449 was the most sensitive bacterium with the lowest MIC value (0.20 mg mL-1, 0.81 mg mL-1) for leaf and root extracts, respectively. The biggest diameter of growth inhibition zone (8±2.44 mm, 9±3.26) compared to Chloramphenicol. Arum leaf extracts showed a synergistic effect with OFX against Escherichia coli and Staphylococcus mutans, and Arum root extracts have a synergistic effect with OFX against Staphylococcus aureus and Staphylococcus mutans. These results displayed that A. maculatum has notable natural bioactive compounds with antioxidant and antibacterial properties.

Keywords

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Bitki Biyokimyası

Bölüm

Araştırma Makalesi

Yazarlar

Mürüvvet Düz ^*
0000-0001-7339-9858
Türkiye

Erken Görünüm Tarihi

21 Ocak 2024

Yayımlanma Tarihi

1 Nisan 2024

Gönderilme Tarihi

27 Aralık 2022

Kabul Tarihi

27 Eylül 2023

Yayımlandığı Sayı

Yıl 2024 Cilt: 27 Sayı: 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1225076

IZ

https://izlik.org/JA27BB48GH

Kaynak Göster

RIS / Bibtex

APA

Düz, M. (2024). In vitro Antimicrobial Activity, Antioxidant Activity and Synergistic/Antagonistic Effect of Arum maculatum L. in Mersin Province, Turkey. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 27(2), 261-272. https://doi.org/10.18016/ksutarimdoga.vi.1225076

Cited By

Folkloric Practices Regarding Goat Diseases and Treatments Among Sarıkeçili Nomads

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

https://doi.org/10.18016/ksutarimdoga.vi.1700665

Arum maculatum L.'nin in vitro Antimikrobiyal Aktivitesi, Antioksidan Aktivitesi ve Sinerjistik/Antagonistik Etkisi

Öz

Anahtar Kelimeler

In vitro Antimicrobial Activity, Antioxidant Activity and Synergistic/Antagonistic Effect of Arum maculatum L. in Mersin Province, Turkey

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

Folkloric Practices Regarding Goat Diseases and Treatments Among Sarıkeçili Nomads

e-ISSN: 2619-9149