Detoxification Efficiency of Micropropagated Alternanthera reineckii Briq. against Zinc Oxide Nanoparticles in Human Keratinocyte Cells

Buğrahan Emsen; İbrahim Çinar; Muhammet Doğan

doi:10.18016/ksutarimdoga.vi.1241907

TR EN

Mikroçoğaltılan Alternanthera reineckii Briq.'nin İnsan Keratinosit Hücrelerinde Çinko Oksit Nanopartiküllerine Karşı Detoksifikasyon Etkinliği

Öz

Nanoteknolojideki hızlı gelişmeler göz önüne alındığında, nanoteknolojinin insan sağlığı üzerindeki tehlikelerinin önüne geçebilmek için nanotoksikoloji alanında bilimsel araştırmalara ihtiyaç duyulmaktadır. Bu amaçla birçok kozmetik üründe yer alan ZnO nanopartikülünün (NP) insan keratinosit hücreleri (HaCaT) üzerindeki sitotoksik etkisini test ettik. Ek olarak, doku kültür yöntemi ile üretilen bir su bitkisi olan Alternanthera reineckii Briq. ile bu sitotoksik etkiyi inhibe etme potansiyelini değerlendirdik. A. reineckii'nin nodal eksplantları, 0,25-1,25 mg/L Thidiazuron (TDZ) ve 0,25 mg/L indol-3-bütirik asit (IBA) kombinasyonlarını içeren Murashige & Skoog bazal ortamında (MS) kültüre edildi. Eksplant başına maksimum sürgün sayısı (22,50 sürgün/eksplant) 0,75 mg/L TDZ+0,25 mg/L IBA içeren kültür ortamında elde edilmiştir. En yüksek sürgün uzunluğu (1,77 cm) 0,25 mg/L TDZ+0,25 mg/L IBA içeren MS ortamında belirlenmiştir. A. reineckii'den Soxhlet ekstraksiyonu yoluyla aseton ve su özütleri elde edilmiştir. Tek başına ZnO'nun HaCaT üzerindeki sitotoksik etkisi, aseton ve su özütleri ile inhibe edilmiştir. ZnO'nun etkisiyle %26,04'e düşen hücre canlılığı, aseton özütü uygulamasıyla %67,83'e kadar yükselmiştir. Genel olarak, sonuçlarımız bu bitkinin ZnO tarafından indüklenen nanotoksisiteye karşı koruyucu potansiyelini ortaya koymuş ve gelecekteki çalışmalara ışık tutmuştur.

Anahtar Kelimeler

Detoxification Efficiency of Micropropagated Alternanthera reineckii Briq. against Zinc Oxide Nanoparticles in Human Keratinocyte Cells

Abstract

Considering the rapid developments in nanotechnology, scientific research in the field of nanotoxicology is required in order to prevent the dangers of nanotechnology on human health. For this purpose, we tested the cytotoxic effect of ZnO nanoparticle (NP), which is included in many cosmetic products, on human keratinocyte cells (HaCaT). In addition, we evaluated to potentially inhibit this cytotoxic effect with an aquatic plant, Alternanthera reineckii Briq. produced by tissue culture method. The nodal explants of A. reineckii were cultured in Murashige & Skoog basal medium (MS) including the combinations of 0.25-1.25 mg/L Thidiazuron (TDZ) and 0.25 mg/L indole-3-butyric acid (IBA). Maximum number of shoots per explant (22.50 shoots/explant) was obtained in the culture medium with 0.75 mg/L TDZ+0.25 mg/L IBA. The highest shoot length (1.77 cm) was determined in MS medium with 0.25 mg/L TDZ+0.25 mg/L IBA. Acetone and water extracts were obtained from A. reineckii through Soxhlet extraction. The cytotoxic effect of ZnO alone on HaCaT was inhibited by acetone and water extracts. The cell viability, which decreased to 26.04% with the effect of ZnO, increased up to 67.83% with the application of acetone extract. Overall, our results revealed the protective potential of this plant against nanotoxicity induced by ZnO and shed light on future studies

Keywords

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Buğrahan Emsen ^*
0000-0002-9636-2596
Türkiye

İbrahim Çinar
0000-0002-0509-913X
Türkiye

Muhammet Doğan
0000-0003-3138-5903
Türkiye

Erken Görünüm Tarihi

27 Mayıs 2023

Yayımlanma Tarihi

31 Ekim 2023

Gönderilme Tarihi

24 Ocak 2023

Kabul Tarihi

14 Mart 2023

Yayımlandığı Sayı

Yıl 2023 Cilt: 26 Sayı: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1241907

IZ

https://izlik.org/JA78EM82YD

Kaynak Göster

RIS / Bibtex

APA

Emsen, B., Çinar, İ., & Doğan, M. (2023). Detoxification Efficiency of Micropropagated Alternanthera reineckii Briq. against Zinc Oxide Nanoparticles in Human Keratinocyte Cells. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 26(5), 1066-1074. https://doi.org/10.18016/ksutarimdoga.vi.1241907

Cited By

Comparison of Antioxidant and Antimicrobial Properties of Zinc oxide and Selenium oxide Nanoparticles using Verbascum kotschyi Boiss. & Hohen.

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

https://doi.org/10.18016/ksutarimdoga.vi.1404682

Mikroçoğaltılan Alternanthera reineckii Briq.'nin İnsan Keratinosit Hücrelerinde Çinko Oksit Nanopartiküllerine Karşı Detoksifikasyon Etkinliği

Öz

Anahtar Kelimeler

Detoxification Efficiency of Micropropagated Alternanthera reineckii Briq. against Zinc Oxide Nanoparticles in Human Keratinocyte Cells

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

Comparison of Antioxidant and Antimicrobial Properties of Zinc oxide and Selenium oxide Nanoparticles using Verbascum kotschyi Boiss. & Hohen.

e-ISSN: 2619-9149