Assessment of Genetic Diversity and Relationships among Gypsophila and Silene Species from Türkiye based on SRAP Markers

Münevver Göçmen; Ayşe Serpil Kaya; Köksal Aydinşakir; Adnan Özçelik; İlknur Polat

doi:10.18016/ksutarimdoga.vi.1398542

TR EN

Türkiye'deki Gypsophila ve Silene Türleri Arasındaki Genetik Çeşitlilik ve İlişkilerin SRAP Belirteçleri Kullanılarak Değerlendirilmesi

Öz

Cipsofilya, Caryophyllaceae familyasının bir üyesi olup cinsi yaklaşık 150 türden oluşur. Bazı türleri, bitkisel ilaç ve gıda gibi çeşitli kullanımlar için ticari olarak yetiştirilmektedir. Dünya çapında en yaygın kesme çiçek olarak kullanılmaktadır. Gypsophila türleri ana genetik kaynak merkezi olan Türkiye'de yaygın olarak bulunmaktadır. Bu çalışmada, ilk olarak Türkiye'deki yerel bölgelerden Gypsophila L. genotipleri toplandı. İkinci olarak, moleküler belirteçler kullanarak genetik çeşitlilik, ıslah programları ve germplazmayı koruma stratejileri için değerli bilgiler elde edildi. Moleküler belirteç olarak dizi ilişkili çoğaltılmış polimorfizm (SRAP), 13 tür içeren (G. viscosa, G. simonii, G. venusta, G. bicolor, G. simulatrix, G. bitlisensis, G. germanicopolitana, G. perfoliata, G. arrostii, G. eleganas, G. paniculata ve G. aucheri) 41 Gypsophila (Caryophyllaceae) genotip ve iki Silene tipin (S. vulgaris L. ve Silene spp.) genetik farklılık ve akrabalık durumunu belirlemek amacıyla kullanılmıştır. Sonuç olarak, 20 primer kombinasyonundan 153 skorlanabilir fragment üretilmiş ve ayrıca tüm belirteçlerr 43 genotip için %100 polimorfizm göstermiştir. Kofenetik korelasyon r değerleri (r ≥ 0,80) hesaplanarak, SRAP belirteçlerinin oluşturduğu dendrogramların önemi bilgiler sunmuştur. Gypsophila ve Silene türleri alt türlere ve bölgelere göre gruplandırılmıştır. Sonuçlar, SRAP belirteçlerinin Gypsophila L. genotipleri arasındaki çeşitliliği ve ilişkileri araştırmak için yararlı olduğunu göstermiştir. Ayrıca, bu veriler ıslah programında yeni Gypsophila L. çeşitlerinin geliştirilmesinde de kullanılabilir.

Anahtar Kelimeler

Assessment of Genetic Diversity and Relationships among Gypsophila and Silene Species from Türkiye based on SRAP Markers

Abstract

Gypsophila is a member of the Caryophyllaceae family and its genus consists of approximately 150 species. Several species are grown commercially, including herbal medicine and food. Its most common use is as a cut flower worldwide. Gypsophila species are native and widely distributed in Türkiye, the main genetic resource center. In this study, Gypsophila L. genotypes were first collected from native areas in Türkiye. Secondly, genetic diversity using molecular markers provided valuable information for breeding programs and strategies of germplasm conservation. Sequence-related amplified polymorphism (SRAP) as a molecular marker was used to determine diversity and relationships among 41 Gypsophila (Caryophyllaceae) genotypes including 13 species (G. viscose, G. simonii, G. venusta, G. bicolor, G. simulator, G. bitlisensis, G. germanicopolitana, G. perfoliata, G. acrostic, G. eleganas, G. paniculata and G. aucheri) and two Silene types (S. vulgaris L. and Silene spp.) as outgroups. Results revealed that twenty primer combinations produced 153 scorable fragments, and all markers showed 100% polymorphism for 43 genotypes. The cophenetic correlation (r = 0.80) between the Dice similarity matrix and the corresponding dendrogram obtained by the SRAP marker revealed good compliance. The Gypsophila and Silene species were grouped according to subspecies and by region. Results indicated that SRAP markers were useful for investigating diversity and relationships among Gypsophila L. germplasm. Additionally, this data could be used to develop new Gypsophila L. varieties in the breeding program.

Keywords

Destekleyen Kurum

TÜBİTAK

Proje Numarası

104O364

Teşekkür

This study is a part of the project numbered 104O364 supported by The Scientific and Technological Research Council of Turkey (TUBITAK). The authors would like to thank TUBITAK for funding. I also thank Prof. Dr. Osman Karagüzel for his assistance in plant material.

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Bahçe Bitkileri Yetiştirme ve Islahı (Diğer)

Bölüm

Araştırma Makalesi

Yazarlar

Münevver Göçmen
0000-0002-8445-2068
Türkiye

Ayşe Serpil Kaya
0000-0001-5236-2562
Türkiye

Köksal Aydinşakir
0000-0003-0225-7646
Türkiye

Adnan Özçelik
0000-0002-5096-775X
Türkiye

İlknur Polat ^*
0000-0001-9841-847X
Türkiye

Erken Görünüm Tarihi

30 Ocak 2025

Yayımlanma Tarihi

12 Şubat 2025

Gönderilme Tarihi

6 Aralık 2023

Kabul Tarihi

6 Mayıs 2024

Yayımlandığı Sayı

Yıl 2025 Cilt: 28 Sayı: 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1398542

IZ

https://izlik.org/JA75UC26NK

Kaynak Göster

RIS / Bibtex

APA

Göçmen, M., Kaya, A. S., Aydinşakir, K., Özçelik, A., & Polat, İ. (2025). Assessment of Genetic Diversity and Relationships among Gypsophila and Silene Species from Türkiye based on SRAP Markers. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 28(1), 83-95. https://doi.org/10.18016/ksutarimdoga.vi.1398542

Cited By

Identification of Fertility and Genetic Diversity of Sugar Beet Polyembryonic Germplasm Resources

Sugar Tech

https://doi.org/10.1007/s12355-025-01669-0

Türkiye'deki Gypsophila ve Silene Türleri Arasındaki Genetik Çeşitlilik ve İlişkilerin SRAP Belirteçleri Kullanılarak Değerlendirilmesi

Öz

Anahtar Kelimeler

Assessment of Genetic Diversity and Relationships among Gypsophila and Silene Species from Türkiye based on SRAP Markers

Abstract

Keywords

Destekleyen Kurum

Proje Numarası

Teşekkür

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

Identification of Fertility and Genetic Diversity of Sugar Beet Polyembryonic Germplasm Resources

e-ISSN: 2619-9149