Yeni Tanımlanan Bir Tür Olan Aubrieta alshehbazii Dönmez, Uğurlu ve M.A. Koch’ un Antikarsinojenik Etkilerinin, Antioksidan Özelliklerinin ve Fitokimyasal Bileşenlerinin Tespiti

Laçine Aksoy; Ömer Hazman; Mustafa Abdullah Yılmaz; Ahmet Büyükben; Mustafa Kargıoğlu; Beyza Çakal; Beyza Gümüş; Feyza Efiloğlu

doi:10.18016/ksutarimdoga.vi.1616833

EN TR

Determination of the Anticarcinogenic Effects, Antioxidative Features and Phytochemical Components of a Newly Identified Species Aubrieta alshehbazii Dönmez, Uğurlu & M.A. Koch

Abstract

Aubrieta alshehbazii is an endemic species known as Elşahbaz Obrizyası. A study was conducted to determine the antioxidative, anticarcinogenic effects and phenolic substance content of this species, which is not well-studied due to its new definition. DPPH radical scavenging effect, total antioxidant and oxidant capacity were measured to determine its antioxidant properties. Healthy HGF cells and lung cancer (A549) cells were used within the scope of the anticarcinogenic effect. Phenolic substances contained in the plant were determined by LC-ESI-MS/MS. As a result of the analysis, it was found that the plant had a statistically significant (p=0.008 and p=0.016) higher DPPH radical scavenging effect than the synthetic antioxidant BHT at 0.1 µM and 0.5 µM concentrations. The total antioxidant capacity of the species is lower than standard antioxidants. While no effects of the extract on A549 cells were observed at low concentrations, it was determined that it had a cytotoxic effect on both HGF cells and A549 cells at high concentrations. As a result of the phenolic content analysis, the most abundant components in its structure are fumaric acid, caffeic acid, quercetin, quinic acid, rosmarinic acid and chlorogenic acid. As a result, the species stands out with its radical scavenging effect. It is recommended that studies be conducted to determine which component is responsible for this effect.

Keywords

Yeni Tanımlanan Bir Tür Olan Aubrieta alshehbazii Dönmez, Uğurlu ve M.A. Koch’ un Antikarsinojenik Etkilerinin, Antioksidan Özelliklerinin ve Fitokimyasal Bileşenlerinin Tespiti

Öz

Aubrieta alshehbazii, Elşahbaz obrizyası olarak bilinen endemik bir türdür. Tanımlanması yeni yapıldığından hakkında pek çalışma bulunmayan bu türün, antioksidatif, antikanserojenik etkileri ile fenolik madde içeriğinin belirlenmesi amacıyla çalışma gerçekleştirilmiştir. Antioksidatif özelliğinin belirlenmesi için DPPH radikal savıcı etkisi, total antioksidan ve oksidan kapasitesi ölçülmüştür. Antikanserojenik etki kapsamında sağlıklı HGF hücreleri ve akciğer kanser (A549) hücreleri kullanılmıştır. Bitkinin içerdiği fenolik maddeler ise LC-ESI-MS/MS ile belirlenmiştir. Analizler sonucunda bitkinin 0.1 µM ve 0.5 µM konsantrasyonda sentetik antioksidan olan BHT den istatistiksel anlamlılıkta (sırasıyla p= 0.008 ve p=0.016)) yüksek bir DPPH radikali savıcı etkiye sahip bulunmuştur. Türün total antioksidan kapasitesi standart antioksidanlardan düşüktür. Düşük konsantrasyonlarda ekstraktın A549 hücreleri üzeirne etkileri görülmezken, yüksek konsantrasyonlarda hem HGF hücreleri ve hem de A549 hücreleri üzerine sitotoksik etki gösterdiği belirlenmiştir. Fenolik içerik analizleri sonucunda yapısında en çok bulunan bileşenler, fumarik asit, kafeik asit, kuersetin, kinik asit, rosmarinik asit ve klorojenik asittir. Sonuç olarak tür radikal savıcı etkisi ile ön plana çıkmaktadır. Bu etkinin hangi bileşenden kaynaklandığının belirlenmesine yönelik çalışmalar yapılması önerilmektedir.

Anahtar Kelimeler

Kaynakça

Al-Shehbaz, I. A., Beilstein, M. A. & Kellogg, E. A. (2006). Systematics and phylogeny of the Brassicaceae (Cruciferae): an overview. Plant Systematics and Evolution, 259, 89-120. https://doi.org/10.1007/s00606-006-0415-z
Al-Shehbaz, I.A., Mutlu, B. & Dönmez, A.A. (2007). The Brassicaceae (Cruciferae) of Turkey, updated. Turkish Journal of Botany, 31(4), 327-336.
Ancev, M., & Goranova, V. (2009). Aubrieta (Brassicaceae) in the Bulgarian flora. Phytologia Balcanica, 15(1), 43-50.
Awika J. M., Rooney L. W., Wu X. L., Prior R. L., & Cisneros-Zevallos L. (2003). Screening methods to measure antioxidant activity of sorghum (Sorghum bicolor) and sorghum products. Journal of Agricultural and Food Chemistry, 51(23), 6657-6662. https://doi.org/10.1021/jf034790i
Balkir, Ş., Hazman, Ö., Aksoy, L., Yılmaz, M. A., Çakır, O., Kara, R. & Erol, İ. (2023). Phytochemical profile, antioxidant and antimicrobial potency of aerial parts of Salvia tomentosa Miller. Acta Chimica Slovenica,70(2), 218-225. 70(2):218-225. https://doi.org/10.17344/acsi.2023.8008
Chahardehi, A. M., Ibrahim, D., & Sulaiman, S. F. (2009). Antioxidant activity and total phenolic content of some medicinal plants in urticaceae family. Journal of Applied Biological Sciences, 3(2), 27-31.
Damasceno, S. S., Dantas, B. B., Ribeiro-Filho, J., Antônio, M. A. D., & Galberto, M. C. J. (2017). Chemical properties of caffeic and ferulic acids in biological system: implications in cancer therapy. a review. Current Pharmaceutical Design, 23(20), 3015-3023. https://doi.org/10.2174/1381612822666161208145508
Dönmez, A. A., Aydın, Z. U., & Koch, M. A. (2017). Aubrieta alshehbazii (Brassicaceae), a new species from Central Turkey. Phytotaxa, 299(1), 103-110. https://doi.org/10.11646/phytotaxa.299.1.8

Erel, O. (2004). A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry, 37(4), 277-285. https://doi.org/10.1016/j.clinbiochem.2003.11.015
Erel, O. (2005). A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38, 1103-1111. https://doi.org/10.1016/j.clinbiochem.2005.08.008
Eruygur, N., Büyükyıldırım, T., Kırcı, D., Ayaz, F., Dogu, S., & Bagcı, Y. (2024). Evaluation of the in vitro enzyme inhibition and antioxidant activity of Clinopodium betulifolium (Boiss. & Balansa) Kuntze. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 27(6), 1282-1288. https://doi.org/10.18016/ ksutarimdoga.vi.1403319
Genaro-Mattos, T.C., Maurício, Â.Q., Rettori, D., Alonso, A., & Hermes-Lima, M. (2015). Antioxidant activity of caffeic acid against iron-induced free radical generation-A chemical approach. PLoS one, 10, e0129963. https://doi.org/10.1371/journal.pone.0129963
Ghorani-Azam, A., Sepahi, S., Riahi-Zanjani, B., Ghamsari, A.A., Mohajeri, S.A., & Balali-Mood, M. (2018). Plant toxins and acute medicinal plant poisoning in children: A systematic literature review. Journal of Research in Medical Sciences, 23, 26. https://doi.org/10.4103/jrms.JRMS_629_17
Greenwell, M., & Rahman, P.K. (2015). Medicinal Plants: Their Use in Anticancer Treatment. International Journal of Pharmaceutical Sciences and Research, 6(10), 4103-4112. https://doi.org/10.13040/IJPSR.0975-8232.6(10).4103-12
Güçlü, G., Ergül, M., Uçar, E., Eruygur, N., Ataş, M., & Akpulat, H.A. (2022). Anticancer, Antioxidant, Antimicrobial and Enzyme Inhibitory Activities of Inula aucheriana. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(5), 946-954. https://doi.org/10.18016/ksutarimdoga.vi.985837
Günay, E., Celik, S., Sarinc-Ulasli, S., Özyürek, A., Hazman, Ö., Günay, S., Özdemir, M., & Ünlü, M. (2016). Comparison of the Anti-inflammatory effects of proanthocyanidin, quercetin, and damnacanthal on benzo(a)pyrene exposed A549 alveolar cell line. Inflammation, 39(2), 744-751. https://doi.org/10.1007/s10753-015-0301-3
Görmez, G., Yüksek, V., Usta, A., Dede, S., & Gümüş, S. (2024). Phenolic Contents, Antioxidant Activities, LC MS Profiles of Mespilus germanica Leaf Extract and Effects on mRNA Transcription Levels of Apoptotic, Autophagic, and Necrotic Genes in MCF7 and A549 Cancer Cell Lines. Cell Biochemistry and Biophysics, 82(3), 2141-2155. https://doi.org/10.1007/s12013-024-01321-w
Hazman, Ö., Aksoy, L., Büyükben, A., Kara, R., Kargioğlu, M., Kumral, Z. B., & Erol, I. (2021). Evaluation of antioxidant, cytotoxic, antibacterial effects and mineral levels of Verbascum lasianthum Boiss. ex Bentham. Anais da Academia Brasileira de Ciências, 93, e20210865. https://doi.org/10.1590/0001-3765202120210865
Karl, R. & Koch, M. A. (2013). A world-wide perspective on crucifer speciation and evolution: phylogeny, biogeography and trait evolution in tribe Arabideae. Annals of Botany, 112(6), 983-1001. https://doi.org/10.1093/aob/mct165
Kaska, A., & Deniz, N., Mammadov, R. (2017). Antioxidative capacities and phenolic compounds of various extracts of Aubrieta deltoidea. Deutscher Wissenschaftsherold, 3, 42-46. https://doi.org/10.19221/2017312
Khan, T., Ali, M., Khan, A., Nisar, P., Jan, S. A., Afridi, S., & Shinwari, Z. K. (2019). Anticancer plants: A review of the active phytochemicals, applications in animal models, and regulatory aspects. Biomolecules, 10(1), 47. https://doi.org/10.3390/biom10010047
Koch, M. & Kiefer, C. (2006). Molecules and migration: biogeographical studies in cruciferous plants. Plant Systematics and Evolution, 259(2-4), 121-142. https://doi.org/10.1007/s00606-006-0416-y
Li, A. S. H., Bandy, B., Tsang, S. S., Davison, A. J. (2000). DNA-breaking versus DNA-protecting activity of four phenolic compounds in vitro. Free Radical Research 33, 551-566. https://doi.org/10.1080/10715760000301091
Lin, C. L., Chen, R. F., Chen, J. Y. F., Chu, Y. C., Wang, H. M., Chou, H. L., Chang, W. C., Fong, Y., Chang, W. T., Wu, C. Y., & Chiu, C. C. (2012). protective effect of caffeic acid on paclitaxel induced anti-proliferation and apoptosis of lung cancer cells involves NF-kappa B pathway. International Journal of Molecular Sciences, 13, 6236-6245. https://doi.org/10.3390/ijms13056236
Mandrich, L., & Caputo, E. (2020). Brassicaceae-derived anticancer agents: Towards a green approach to beat cancer. Nutrients,12(3), 868.
Miceli, N., Cavò, E., Ragusa, M., Cacciola, F., Mondello, L., Dugo, L., Acquaviva, R., Malfa, G. A., Marino, A., D’Arrigo, M., & Taviano, M. F. (2020). Brassica incana Ten. (Brassicaceae): Phenolic constituents, antioxidant and cytotoxic properties of the leaf and flowering top extracts. Molecules, 25(6), 1461. https://doi.org/10.3390/molecules25061461
Min, J., Shen, H., Xi, W., Wang, Q., Yin, L., Zhang, Y. F., Yu, Y., Yang, Q., & Wang, Z. N. (2018). Synergistic anticancer activity of combined use of caffeic acid with paclitaxel enhances apoptosis of non-small-cell lung cancer H1299 cells in vivo and in vitro. Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, 48, 1433-1442. https://doi.org/10.1159/000492253
Molole, G. J., Gure, A. & Abdissa, N. (2022). Determination of total phenolic content and antioxidant activity of Commiphora mollis (Oliv.) Engl. resin. BMC Chemistry, 16, 48. https://doi.org/10.1186/s13065-022-00841-x
Mutlu, B. & Karakuş, Ş. (2015). A new species of Sisymbrium (Brassicaceae) from Turkey: Morphological and molecular evidences. Turkish Journal of Botany, 39, 325-333. https://doi.org/10.3906/bot-1404-28
Neamtu, A. A., Maghiar, T. A., Alaya, A., Olah N. K., Turcus V., Pelea, D., Totolici, B. D., Neamtu, C., Maghiar, A. M., & Mathe, E. (2022). A comprehensive view on the quercetin impact on colorectal cancer. Molecules, 27(6), 1873. https://doi.org/10.3390/molecules27061873
Pal, S. K., & Shukla, Y. (2003). Herbal medicine: Current status and the future. Asian Pacific Journal of Cancer Prevention, 4, 281-288.
Prior, R. L., Wu, X., & Schaich, K. (2005). Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry, 53(10), 4290-4302. https://doi.org/10.1021/jf0502698
Reyes-Farias, M., & Carrasco-Pozo, C. (2019). The anti-cancer effect of quercetin: molecular implications in cancer metabolism. International Journal of Molecular Sciences, 20(13), 3177. https://doi.org/10.3390/ijms20133177
Sidoryk, K., Jaromin, A., Filipczak, N., Cmoch, P., & Cybulski, M. (2018). Synthesis and antioxidant activity of caffeic acid derivatives. Molecules, 23, 2199. https://doi.org/10.3390/molecules23092199
Silva, T., Oliveira, C., & Borges, F. (2014). Caffeic acid derivatives, analogs and applications: A patent review (2009-2013). Expert Opinion on Therapeutic Patents, 24(11), 1257-1270. https://doi.org/10.1517/13543776.2014.959492
Slinkard, K., & Singleton V. L. (1977). Total Phenol Analysis: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28, 49-55. https://doi.org/10.5344/ajev.1977.28.1.49
Son, S., & Lewis, B.A. (2002). Free radical scavenging and antioxidative activity of caffeic acid amide and ester analogues: structure–activity relationship. Journal of Agricultural and Food Chemistry, 50, 468-472. https://doi.org/10.1021/jf010830b
Tang S. M., Deng X. T., Zhou J., Li Q. P., Ge X. X., & Miao L. (2020). Pharmacological basis and new insights of quercetin action in respect to its anti-cancer effects. Biomedicine & Pharmacotherapy, 121, 109604. https://doi.org/10.1016/j.biopha.2019.109604
Tosovic, J. (2017). Spectroscopic features of caffeic acid: Theoretical study. Kragujevac Journal of Science, 39, 99-108.
Xu, D. P., Li, Y., Meng, X., Zhou, T., Zhou, Y., Zheng, J., Zhang, J. J., & Li, H. B. (2017). Natural Antioxidants in Foods and Medicinal Plants: Extraction, Assessment and Resources. International Journal of Molecular Sciences, 18(1), 96. https://doi.org/10.3390/ijms18010096
Yılmaz, M. A. (2020). Simultaneous quantitative screening of 53 phytochemicals in 33 species of medicinal and aromatic plants: A detailed, robust and comprehensive LC-MS/MS method validation. Industrial Crops and Products, 149, 112347. https://doi.org/10.1016/j.indcrop.2020.112347
Yüzbaşıoğlu, S., Koch, M. A. & Al-Shehbaz, I. A. (2015). Proof of a knowledge database concept. Aubrieta ekimii (Brassicaceae), a new species from NW Anatolia (Turkey): morphological and molecular support. Plant Systematics And Evolution, 301, 2043-2055. https://doi.org/10.1007/s00606-015-1212-3

Ayrıntılar

Birincil Dil

Türkçe

Konular

Bitki Biyokimyası

Bölüm

Araştırma Makalesi

Yazarlar

Laçine Aksoy ^*
0000-0001-8086-5079
Türkiye

Ömer Hazman
0000-0002-2702-6847
Türkiye

Mustafa Abdullah Yılmaz
0000-0002-4090-7227
Türkiye

Ahmet Büyükben
0000-0002-5639-7217
Türkiye

Mustafa Kargıoğlu
0000-0003-0386-9716
Türkiye

Beyza Çakal
0009-0004-3244-7189
Türkiye

Beyza Gümüş
0009-0009-1437-0321
Türkiye

Feyza Efiloğlu
0009-0004-7443-6357
Türkiye

Erken Görünüm Tarihi

1 Mayıs 2025

Yayımlanma Tarihi

9 Haziran 2025

Gönderilme Tarihi

10 Ocak 2025

Kabul Tarihi

18 Mart 2025

Yayımlandığı Sayı

Yıl 2025 Cilt: 28 Sayı: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1616833

IZ

https://izlik.org/JA44TL46GN

Kaynak Göster

RIS / Bibtex

APA

Aksoy, L., Hazman, Ö., Yılmaz, M. A., Büyükben, A., Kargıoğlu, M., Çakal, B., Gümüş, B., & Efiloğlu, F. (2025). Yeni Tanımlanan Bir Tür Olan Aubrieta alshehbazii Dönmez, Uğurlu ve M.A. Koch’ un Antikarsinojenik Etkilerinin, Antioksidan Özelliklerinin ve Fitokimyasal Bileşenlerinin Tespiti. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 28(3), 650-660. https://doi.org/10.18016/ksutarimdoga.vi.1616833

Determination of the Anticarcinogenic Effects, Antioxidative Features and Phytochemical Components of a Newly Identified Species Aubrieta alshehbazii Dönmez, Uğurlu & M.A. Koch

Abstract

Keywords

Yeni Tanımlanan Bir Tür Olan Aubrieta alshehbazii Dönmez, Uğurlu ve M.A. Koch’ un Antikarsinojenik Etkilerinin, Antioksidan Özelliklerinin ve Fitokimyasal Bileşenlerinin Tespiti

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

e-ISSN: 2619-9149