Morphological and Molecular Characterization of Terfezia claveryi and its Distribution in Türkiye

Ilgaz Akata; Eda Kumru; Gülce Ediş; Beste Gizem Özbey; İsmail Acar; Ergin Sahin

doi:10.18016/ksutarimdoga.vi.1622161

TR EN

Morphological and Molecular Characterization of Terfezia claveryi and its Distribution in Türkiye

Öz

The samples were collected from Ankara, Gaziantep, Konya, Niğde, and Şanlıurfa (Türkiye), between 2020 and 2023. Morphological analyses and nrITS rDNA sequence-based phylogenetic methods were used to examine the samples. Twenty-one specimens displayed morphological features consistent with Terfezia claveryi Chatin at both macro and micro levels. Genetic analysis revealed over 99% sequence similarity with this species. Detailed documentation included habitat descriptions, geographical coordinates, collection dates, and photographic records of macroscopic and microscopic structures. These findings contribute valuable insights into the distribution and taxonomy of T. claveryi in Türkiye.

Anahtar Kelimeler

Proje Numarası

Ankara Üniversitesi BAP projesi, Proje no:FBA-2022-2383

Etik Beyan

Bu çalışmada sorun teşkil edecek etik bir durum yoktur

Teşekkür

Ankara Üniversitesi

Kaynakça

Abdelaziz, M. (2018). Discovering the environmental factors affecting the distribution of Terfezia claveryi Chatin in the Northwest of the Region of Murcia. [Master thesis, Lleida, University of Lleida, p 31].
Aish, A. A., Abdulmalek, S. T., Kareem, T. A., Yasir, L. B., & Matny, O. (2020). Molecular identification and genetic diversity study of the Iraqi truffles. Journal of Phytology, 12, 121-126. https://doi.org/10.25081/jp.2020.v12.6599
Akata, I., & Erdoğdu, M. (2020). First report of Rutstroemia elatina (Ascomycota) from Turkey. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23(2), 391-395. https://doi.org/10.18016/ ksutarimdoga.vi. 626466
Akata, I., Şen, İ., Sevindik, M. & Kabaktepe, Ş. (2022). Truffle checklist of Turkey II with a new record. Turkish Journal of Agriculture-Food Science and Technology, 10(10), 1913-1920. https://doi.org/10.24925/ turjaf.v10i10.1913-1920.5482
Akata, I., Edis, G., Ozbey, B. G., Keskin, E., & Sahin, E. (2024a). Complete Genome Analyses of a Novel Flexivirus with Unique Genome Organization and Three Endornaviruses Hosted by the Mycorrhizal Fungus Terfezia claveryi. Current Microbiology, 81(7), 1-11. https://doi.org/10.1007/s00284-024-03745-2
Akata, I., Kumru, E., Sahin, E., Acar, I., & Kaya, E. (2024b). Amanita vidua: A new record for Turkish Amanita Section Phalloideae based on morphological and molecular data. Trakya University Journal of Natural Sciences, 25(1), 97-110. https://doi.org/10.23902/trkjnat.1446327
Akata, I., Ediş, G., Kumru, E., Acar, İ. & Şahin, E. (2024c). Initial Report of Inocybe costinitii in Türkiye with Morphological and Molecular Data. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 27(6), 1304-1312. https://doi.org/10.18016/ksutarimdoga.vi.1467628
Akata, I., Ediş, G., Kumru, E., Acar, İ., & Sahin, E. (2024d). Taxonomic Studies on Rhodocybe asyae Specimens Discovered in a New Location. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 27(Suppl 1), 124-132. https://doi.org/10.18016/ksutarimdoga.vi.1507554

AlAhmed, A., & Khalil, H. E. (2019). Antidiabetic‎ Activity of Terfezia Claveryi; An In Vitro and In Vivo Study. Biomedical and Pharmacology Journal, 12(2), 603-608. https://dx.doi.org/10.13005/bpj/1680
Akyüz, M., Kirbağ, S., & Kurşat, M. (2012). Ecological aspects of the arid and semi-arid truffle in Turkey: evaluation of soil characteristics, morphology, distribution, and mycorrhizal relationships. Turkish Journal of Botany, 36(4), 386-391. https://doi.org/10.3906/bot-1103-4
Akyüz, M., Kırbağ, S., & Bircan, B. (2015a). Medical characteristics of arid-semi arid Truffle (Terfezia and Picoa) in the Elazığ-Malatya region of Turkey. Hacettepe Journal of Biology and Chemistry, 43(4), 301-308. https://doi.org/10.15671/HJBC.20154315989
Akyüz, M., Kirbag, S., Bircan, B., & Gurhan, Y. (2015b). Diversity and distribution of arid-semi arid truffle (Terfezia and Picoa) in Elazig-Malatya region of Turkey. Mycosphere Journal of Fungal Biology, 6(6): 766–783. https://doi.org/10.5943/mycosphere/6/6/11
Alsheikh, A. M. (1994). Taxomony and mycorrhizal ecology of the desert truffles in the genus Terfezia. [Doktoral Thesis, Corvallis, Oregon State University, p 239].
Behzadi, A. A., Zareie, M., Abbasi, A., Masoumi, B., Ashrafi-Dehkordi, E., & Morte, A. (2021). Physicochemical properties, nutritional composition, and phylogenic analysis of black truffles grown in Fars Province, Iran. International Journal of Nutrition Sciences, 6(1), 45-51. https://doi.org/10.30476/IJNS.2021.90183.1128
Bordallo, J. J., Rodríguez, A., Kounas, V., Camello, F., Honrubia, M., & Morte, A. (2015). Two new Terfezia species from southern Europe. Phytotaxa, 230(3), 239-249. https://doi.org/10.11646/phytotaxa.230.3.2
Bordallo, J. J., Rodríguez, A., Muñoz-Mohedano, J. M., Suz, L. M., Honrubia, M., & Morte, A. (2013). Five new Terfezia species from the Iberian Peninsula. Mycotaxon, 124(1), 189-208. https://doi.org/10.5248/124.189
Dafri, A., & Beddiar, A. (2017). Desert truffles from northeastern Algerian coasal dunes: Ecology, identification and symbiosis. Journal of Fundamental and Applied Sciences, 9(1), 154-169. http://dx.doi.org/10.4314/jfas.v9i1.11
Dahham, S. S., Al-Rawi, S. S., Ibrahim, A. H., Majid, A. S. A., & Majid, A. M. S. A. (2018). Antioxidant, anticancer, apoptosis properties and chemical composition of black truffle Terfezia claveryi. Saudi Journal of Biological Sciences, 25(8), 1524-1534. https://doi.org/10.1016/j.sjbs.2016.01.031
Díez, J., Manjón, J. L., & Martin, F. (2002). Molecular phylogeny of the mycorrhizal desert truffles (Terfezia and Tirmania), host specificity and edaphic tolerance. Mycologia, 94(2), 247-259. https://doi.org/10.1080/15572536.2003.11833230
Felsenstein, J. (1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39(4), 783-791. https://doi.org/10.2307/2408678
Ferdman, Y., Aviram, S., Nurit, R. B., Trappe, J. M., & Kagan-Zur, V. (2005). Phylogenetic studies of Terfezia pfeilii and Choiromyces echinulatus (Pezizales) support new genera for southern African truffles: Kalaharituber and Eremiomyces. Mycological Research, 109(2), 237-245. https://doi.org/10.1017/S0953756204001789
Jamali, S., & Banihashemi, Z. (2012). Hosts and distribution of desert truffles in Iran, based on morphological and molecular criteria. Journal of Agricultural Science & Technology, 14(6), 1379-1396.
Janakat, S., & Nassar, M. (2010). Hepatoprotective activity of desert truffle (Terfezia claveryi) in comparison with the effect of Nigella sativa in the rat. Pakistan Journal of Nutrition, 9(1), 52-56. https://doi.org/10.3923/pjn.2010.52.56
Kimura, M. (1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111-120. https://doi.org/10.1007/BF01731581
Kovács, G. M., Balazs, T. K., Calonge, F. D., & Martin, M. P. (2011). The diversity of Terfezia desert truffles: new species and a highly variable species complex with intrasporocarpic nrDNA ITS heterogeneity. Mycologia, 103(4), 841-853. https://doi.org/10.3852/10-312
Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547-1549. https://doi.org/10.1093/molbev/msy096
Lu, B., Zhang, F. M., Yu, F. Q., & Rinaldi, A. C. (2022). Ethnobiological notes and volatile profiles of two rare Chinese desert truffles. Mycology, 13(3), 177-184. https://doi.org/10.1080/21501203.2022.2035005
Malik, H. M., Munazza Gull, M. G., Ulfat Omar, U. O., Kumosani, T. A., Al-Hejin, A. M., Marzouki, H. Z., & Iyer, A. P. (2018). Evaluation of the antibacterial potential of desert truffles (Terfezia spp) extracts against Methicillin Resistant Staphylococcus aureus (MRSA). Journal of Experimental Biology and Agricultural Sciences, 6, 652–660. https://doi.org/10.18006/2018.6(4).652.660
Mandeel, Q. A., & Al-Laith, A. A. A. (2007). Ethnomycological aspects of the desert truffle among native Bahraini and non-Bahraini peoples of the Kingdom of Bahrain. Journal of Ethnopharmacology, 110(1), 118-129. https://doi.org/10.1016/j.jep.2006.09.014
Marian, I. M., Valdes, I. D., Hayes, R. D., LaButti, K., Duffy, K., Chovatia, M., Johnson, J., Ng, V., Lugones, L. G., Wösten, H. A. B., Grigoriev, I. V., & Ohm, R. A. (2024). High phenotypic and genotypic plasticity among strains of the mushroom-forming fungus Schizophyllum commune. Fungal Genetics and Biology, 173, 103913. https://doi.org/10.1016/j.fgb.2024.103913
Marasas, W. F. O. & Trappe, J.M. (1973). Notes on Southern African Tuberales. Bothalia 11, 139-141. https://doi.org/10.4102/abc.v11i1/2.1994
Moreno, G., Diez, J., & Manjon, J. L. (2002). Terfezia boudieri, first records from Europe of a rare vernal hypogeous mycorrhizal fungus. Persoonia-Molecular Phylogeny and Evolution of Fungi, 17(4), 637-641.
Mostowfizadeh-Ghalamfarsa, R., Jamali, S., & Banihashemi, Z. (2010). Molecular phylogeny of three desert truffles from Iran based on ribosomal genome. Rostaniha, 11(2), 151-162.
Radhouani, F., Zampieri, E., Guasmi, F., Ferchichi, A., & Mello, A. (2019). Assessment of β-tubulin gene as a complementary molecular marker for desert truffle identification: A study case on Tunisian samples. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 153(1), 19-24. https://doi.org/10.1080/11263504.2018.1435571
Raja, H.A., Miller, A.N., Pearce, C.J. & Oberlies, N.H. (2017). Fungal identification using molecular tools: a primer for the natural products research community. Journal of Natural Products. 80(3), 756-770. https://doi.org/10.1021/acs.jnatprod.6b01085
Rogers, S.O., Bendich, A.J. (1994). Extraction of total cellular DNA from plants, algae and fungi. In: Gelvin, S.B., Schilperoort, R.A. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0511-8_12
Sahin, E., Ozbey Saridogan, B. G., Keskin, E., & Akata, I. (2023). Identification and complete genome sequencing of a novel betapartitivirus naturally infecting the mycorrhizal desert truffle Terfezia claveryi. Virus Genes, 59(2), 254-259. https://doi.org/10.1007/s11262-023-01972-6
Santos-Silva, C., Louro, R., Natário, B., & Nobre, T. (2021). Lack of knowledge on ecological determinants and cryptic lifestyles hinder our understanding of Terfezia diversity. MycoKeys, 84, 1-14. https://doi.org/10.3897/mycokeys.84.71372
Sbissi, I., Ghodhbane-Gtari, F., Neffati, M., Ouzari, H., Boudabous, A., & Gtari, M. (2011). Diversity of the desert truffle Terfezia boudieri Chatin. in southern Tunisia. Canadian Journal of Microbiology, 57(7), 599-605. https://doi.org/10.1139/w11-040
Türkoğlu A., Castellano M. A., Trappe J. M., & Güngör M. Y. (2015). Turkish truffles I: 18 new records for Turkey. Turkish Journal of Botany, 39(2), 359-376. https://doi.org/10.3906/bot-1406-42
Türkoğlu A., & Castellano M. A. (2014). New Records of Some Ascomycete Truffle Fungi from Turkey. Turkish Journal of Botany, 38, 406-416. https://doi.org/10.3906/bot-1303-24
Veeraraghavan, V. P., Hussain, S., Papayya Balakrishna, J., Dhawale, L., Kullappan, M., Mallavarapu Ambrose, J., & Krishna Mohan, S. (2021). A Comprehensive and Critical Review on Ethnopharmacological Importance of Desert Truffles: Terfezia claveryi, Terfezia boudieri, and Tirmania nivea. Food Reviews International, 38(sup1), 846–865. https://doi.org/10.1080/87559129.2021.1889581
White, T.J., Bruns, T., Lee, S. & Taylor, J.W. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. pp. 315- 322. In: Innis, M.A. & Gelfand, D.H. (eds). PCR Protocols: A Guide to Methods and Applications. Academic Press, London, 482 pp.
Zambonelli, A., Donnini, D., Rana, G. L., Fascetti, S., Benucci, G. M. N., Iotti, M., Khabar L,, Bawadekji, A, Piattoni, F., Campagno, R., Venturella, G. (2014). Hypogeous fungi in Mediterranean maquis, arid and semi-arid forests. Plant Biosystems-An International Journal Dealing With all Aspects of Plant Biology, 148(2), 392-401. https://doi.org/10.1080/11263504.2013.877537
Zitouni-Haouar, F. E. H., Carlavilla, J. R., Moreno, G., Manjon, J. L., & Fortas, Z. (2018). Genetic diversity of the genus Terfezia (Pezizaceae, Pezizales): New species and new record from North Africa. Phytotaxa, 334(2), 183-194. https://doi.org/10.11646/phytotaxa.334.2.7

Ayrıntılar

Birincil Dil

İngilizce

Konular

Bitki Bilimi (Diğer)

Bölüm

Araştırma Makalesi

Yazarlar

Ilgaz Akata ^*
0000-0002-1731-1302
Türkiye

Eda Kumru
0009-0000-7417-6197
Türkiye

Gülce Ediş
0000-0001-7038-9865
Türkiye

Beste Gizem Özbey
0000-0002-9777-6598
Türkiye

İsmail Acar
0000-0002-6049-4896
Türkiye

Ergin Sahin
0000-0003-1711-738X
Türkiye

Erken Görünüm Tarihi

1 Mayıs 2025

Yayımlanma Tarihi

9 Haziran 2025

Gönderilme Tarihi

17 Ocak 2025

Kabul Tarihi

6 Mart 2025

Yayımlandığı Sayı

Yıl 2025 Cilt: 28 Sayı: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1622161

IZ

https://izlik.org/JA55XL36GE

Kaynak Göster

RIS / Bibtex

APA

Akata, I., Kumru, E., Ediş, G., Özbey, B. G., Acar, İ., & Sahin, E. (2025). Morphological and Molecular Characterization of Terfezia claveryi and its Distribution in Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 28(3), 843-854. https://doi.org/10.18016/ksutarimdoga.vi.1622161

AMA

1.Akata I, Kumru E, Ediş G, Özbey BG, Acar İ, Sahin E. Morphological and Molecular Characterization of Terfezia claveryi and its Distribution in Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2025;28(3):843-854. doi:10.18016/ksutarimdoga.vi.1622161

Chicago

Akata, Ilgaz, Eda Kumru, Gülce Ediş, Beste Gizem Özbey, İsmail Acar, ve Ergin Sahin. 2025. “Morphological and Molecular Characterization of Terfezia claveryi and its Distribution in Türkiye”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 28 (3): 843-54. https://doi.org/10.18016/ksutarimdoga.vi.1622161.

EndNote

Akata I, Kumru E, Ediş G, Özbey BG, Acar İ, Sahin E (01 Haziran 2025) Morphological and Molecular Characterization of Terfezia claveryi and its Distribution in Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 28 3 843–854.

IEEE

[1]I. Akata, E. Kumru, G. Ediş, B. G. Özbey, İ. Acar, ve E. Sahin, “Morphological and Molecular Characterization of Terfezia claveryi and its Distribution in Türkiye”, Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, c. 28, sy 3, ss. 843–854, Haz. 2025, doi: 10.18016/ksutarimdoga.vi.1622161.

ISNAD

Akata, Ilgaz - Kumru, Eda - Ediş, Gülce - Özbey, Beste Gizem - Acar, İsmail - Sahin, Ergin. “Morphological and Molecular Characterization of Terfezia claveryi and its Distribution in Türkiye”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 28/3 (01 Haziran 2025): 843-854. https://doi.org/10.18016/ksutarimdoga.vi.1622161.

JAMA

1.Akata I, Kumru E, Ediş G, Özbey BG, Acar İ, Sahin E. Morphological and Molecular Characterization of Terfezia claveryi and its Distribution in Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2025;28:843–854.

MLA

Akata, Ilgaz, vd. “Morphological and Molecular Characterization of Terfezia claveryi and its Distribution in Türkiye”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, c. 28, sy 3, Haziran 2025, ss. 843-54, doi:10.18016/ksutarimdoga.vi.1622161.

Vancouver

1.Ilgaz Akata, Eda Kumru, Gülce Ediş, Beste Gizem Özbey, İsmail Acar, Ergin Sahin. Morphological and Molecular Characterization of Terfezia claveryi and its Distribution in Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 01 Haziran 2025;28(3):843-54. doi:10.18016/ksutarimdoga.vi.1622161