Geographical Variations in Physicochemical and Antioxidant Properties of Propolis from Meshgin Apiaries, Iran

Maghsoud Besharatı; Boukaga Farmani; Deniz Azhir

doi:10.18016/ksutarimdoga.vi.1841549

EN TR

Geographical Variations in Physicochemical and Antioxidant Properties of Propolis from Meshgin Apiaries, Iran

Abstract

Propolis is a natural resinous substance produced by honeybees. Its physicochemical and antioxidant properties vary significantly with geographical origin, as shown in this study using samples from three Meshgin apiaries (Qara Dagh, Meshgin, and Yilagh). Collected during spring 2024, the propolis was analyzed for moisture, pH, ash, soluble/insoluble solids, phenolic, flavonoid, and tannin content, alongside antioxidant activity and mineral composition. Results revealed consistent physicochemical parameters across samples, with dry matter exceeding 98% and ash content ranging 3.18–3.92%, aligning with national standards. However, Meshgin propolis displayed superior bioactive properties, including the highest total phenolic (18,402.1 µg GAE/g) and flavonoid content (1,575.3 µg QE/g), alongside elevated tannin levels (7275.6 µg GAE/g) and antioxidant activity (91.33% DPPH inhibition, 73.52% ABTS inhibition), attributed to the region’s rich biodiversity of medicinal flora. Mineral analysis highlighted significant geographical variations in nickel, copper, phosphorus, and calcium concentrations, with Yilagh showing the highest calcium content (69.90 mg/kg). These findings underscore the profound influence of local botanical sources and environmental conditions on propolis composition, positioning region-specific propolis, especially from Meshgin, as a potent natural resource for pharmaceutical and nutraceutical applications.

Keywords

Geographical Variations in Physicochemical and Antioxidant Properties of Propolis from Meshgin Apiaries, Iran

Öz

Propolis is a natural resinous substance produced by honeybees. Its physicochemical and antioxidant properties vary significantly with geographical origin, as shown in this study using samples from three Meshgin apiaries (Qara Dagh, Meshgin, and Yilagh). Collected during spring 2024, the propolis was analyzed for moisture, pH, ash, soluble/insoluble solids, phenolic, flavonoid, and tannin content, alongside antioxidant activity and mineral composition. Results revealed consistent physicochemical parameters across samples, with dry matter exceeding 98% and ash content ranging 3.18–3.92%, aligning with national standards. However, Meshgin propolis displayed superior bioactive properties, including the highest total phenolic (18,402.1 µg GAE/g) and flavonoid content (1,575.3 µg QE/g), alongside elevated tannin levels (7275.6 µg GAE/g) and antioxidant activity (91.33% DPPH inhibition, 73.52% ABTS inhibition), attributed to the region’s rich biodiversity of medicinal flora. Mineral analysis highlighted significant geographical variations in nickel, copper, phosphorus, and calcium concentrations, with Yilagh showing the highest calcium content (69.90 mg/kg). These findings underscore the profound influence of local botanical sources and environmental conditions on propolis composition, positioning region-specific propolis, especially from Meshgin, as a potent natural resource for pharmaceutical and nutraceutical applications.

Anahtar Kelimeler

Etik Beyan

This study did not involve human participants or live animals. Collection of propolis from apiaries was conducted with the consent of beekeepers and did not cause harm to honeybee colonies.

Kaynakça

Alencar, S. M., Oldoni, T. L. C., Castro, M. L., et al. (2007). Chemical composition and biological activity of a new type of Brazilian propolis: Red propolis. Journal of Ethnopharmacology, 113(2), 278–283. https://doi.org/10.1016/j.jep.2007.06.005
Allan, R. (2010). Antibacterial activity of propolis and honey against Staphylococcus aureus and Escherichia coli. African Journal of Microbiology Research, 4(17), 1872–1878.
Bankova, V., Popova, M., Bogdanov, S., & Sabatini, A. G. (2002). Chemical composition of European propolis: Expected and unexpected results. Zeitschrift für Naturforschung C, 57(5–6), 530–533. https://doi.org/10.1515/znc-2002-5-622
Bankova, V., Popova, M., & Trusheva, B. (2014). Propolis volatile compounds: Chemical diversity and biological activity. Chemical Central Journal, 8, Article 28. https://doi.org/10.1186/1752-153X-8-28
Banskota, A. H., Tezuka, Y., & Kadota, S. (2001). Recent progress in pharmacological research of propolis. Phytotherapy Research, 15(7), 561–571. https://doi.org/10.1002/ptr.1029
Besharati, M., Gholamalipour, A., Taghizadeh, A., Azhir, D., & Lackner, M. (2024). Physicochemical characteristics and antioxidant attributes of propolis sourced from various regions in Iran. Applied Food Research, 4, Article 100603. https://doi.org/10.1016/j.afres.2024.100603
Çoban, Ö. E., Fadiloğlu, E. E., & Çoban, M. Z. (2018). Investigation of some quality characteristics of smoked common carp (Cyprinus carpio) sausages supplemented with propolis extract. Ecological Life Sciences, 13(4), 197–203.
da Silva, J. F. M., de Souza, M. C., Matta, S. R., de Andrade, M. R., & Vidal, F. V. N. (2006). Correlation analysis between phenolic levels of Brazilian propolis extracts and their antimicrobial and antioxidant activities. Food Chemistry, 99(3), 431–435. https://doi.org/10.1016/j.foodchem.2005.08.013

Dias, L. G., Pereira, A. P., & Estevinho, L. M. (2012). Comparative study of different Portuguese samples of propolis: Pollinic, sensorial, physicochemical, microbiological characterization and antibacterial activity. Food and Chemical Toxicology, 50(12), 4246–4253. https://doi.org/10.1016/j.fct.2012.08.056
Ehsani, A., Hashemi, M., & Afshari, A. (2017). Antioxidant activity of Echinophora platyloba DC essential oil: A comparative study on four different methods. Iranian Journal of Veterinary Science and Technology, 8(2), 47–52.
González-Martín, M. I., Escuredo, O., Revilla, I., et al. (2015). Determination of the mineral composition and toxic element contents of propolis by near infrared spectroscopy. Sensors, 15(11), 27854–27868. https://doi.org/10.3390/s151127854
Helrich, K. (Ed.). (1990). Official methods of analysis of the Association of Official Analytical Chemists (15th ed.). AOAC.
Hopcroft, N., Barling, P., & Conyers, M. K. (2018). Inductively coupled plasma spectrometry in environmental analysis. Environmental Chemistry Letters, 16(3), 813–826. https://doi.org/10.1007/s10311-017-0673-7
Kasote, D., Bankova, V., & Viljoen, A. M. (2022). Propolis: Chemical diversity and challenges in quality control. Phytochemistry Reviews, 21(6), 1887–1911. https://doi.org/10.1007/s11101-022-09816-0
Koc, A. N., Silici, S., Ercal, B. D., et al. (2007). Antifungal activity of propolis in four different fruit juices. Food Technology and Biotechnology, 45(1), 57–61.
Kocot, J., Kiełczykowska, M., Luchowska-Kocot, D., Kurzepa, J., & Musik, I. (2018). Antioxidant potential of propolis, bee pollen, and royal jelly: Possible medical application. Oxidative Medicine and Cellular Longevity, 2018, Article 7074209. https://doi.org/10.1155/2018/7074209
Koru, O., Toksoy, F., Acikel, C. H., et al. (2007). In vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens. Anaerobe, 13(3–4), 140–145. https://doi.org/10.1016/j.anaerobe.2007.02.001
Krishnaiah, D., Devi, T., Bono, A., & Sarbatly, R. (2009). Studies on phytochemical constituents of six Malaysian medicinal plants. Journal of Medicinal Plants Research, 3(2), 67–72.
Küçük, M., Kolaylı, S., Karaoğlu, Ş., Ulusoy, E., Baltacı, C., & Candan, F. (2007). Biological activities and chemical composition of three honeys of different types from Anatolia. Food Chemistry, 100(2), 526–534. https://doi.org/10.1016/j.foodchem.2005.10.010
Kumazawa, S., Hamasaka, T., & Nakayama, T. (2004). Antioxidant activity of propolis of various geographic origins. Food Chemistry, 84(3), 329–339. https://doi.org/10.1016/S0308-8146(03)00216-4
Mandal, M. D., & Mandal, S. (2011). Honey: Its medicinal property and antibacterial activity. Asian Pacific Journal of Tropical Biomedicine, 1(2), 154–160. https://doi.org/10.1016/S2221-1691(11)60016-6
Nikaido, H. (2003). Molecular basis of bacterial outer membrane permeability revisited. Microbiology and Molecular Biology Reviews, 67(4), 593–656. https://doi.org/10.1128/MMBR.67.4.593-656.2003
Pietta, P. G. (2000). Flavonoids as antioxidants. Journal of Natural Products, 63(7), 1035–1042. https://doi.org/10.1021/np9904509
Popova, M., Bankova, V., Butovska, D., et al. (2004). Validated methods for the quantification of biologically active constituents of poplar-type propolis. Phytochemical Analysis, 15(4), 235–240. https://doi.org/10.1002/pca.777
Rice-Evans, C. A., Miller, N. J., & Paganga, G. (1996). Structure–antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biology and Medicine, 20(7), 933–956. https://doi.org/10.1016/0891-5849(95)02227-9
SAS Institute Inc. (2018). SAS/STAT® user’s guide (Version 8.1). SAS Institute Inc.
Sforcin, J. M. (2016). Biological properties and therapeutic applications of propolis. Phytotherapy Research, 30(6), 894–905. https://doi.org/10.1002/ptr.5598
Socha, R., Juszczak, L., Pietrzyk, S., Gałkowska, D., Fortuna, T., & Witczak, T. (2011). Phenolic profile and antioxidant properties of Polish honeys. International Journal of Food Science & Technology, 46(3), 528–534. https://doi.org/10.1111/j.1365-2621.2010.02518.x
Tanasiewicz, M., Skucha-Nowak, M., Dawid-Pać, R., et al. (2012). Influence of propolis on oral health. Evidence-Based Complementary and Alternative Medicine, 2012, Article 681970. https://doi.org/10.1155/2012/681970
Viuda-Martos, M., Ruiz-Navajas, Y., Fernández-López, J., & Pérez-Álvarez, J. A. (2008). Functional properties of honey, propolis, and royal jelly. Journal of Food Science, 73(9), R117–R124. https://doi.org/10.1111/j.1750-3841.2008.00966.x
Zulhendri, F., Chandrasekaran, K., Kowacz, M., et al. (2021). Antiviral, antibacterial, antifungal, and antiparasitic properties of propolis: A review. Foods, 10(6), Article 1360. https://doi.org/10.3390/foods10061360

Ayrıntılar

Birincil Dil

İngilizce

Konular

Arı ve İpek Böceği Yetiştiriciliği ve Islahı

Bölüm

Araştırma Makalesi

Yazarlar

Maghsoud Besharatı ^*
0000-0002-5233-6425
Iran

Boukaga Farmani
0000-0003-3504-9958
Iran

Deniz Azhir
0000-0000-0000-0001
Iran

Erken Görünüm Tarihi

11 Nisan 2026

Yayımlanma Tarihi

-

Gönderilme Tarihi

16 Aralık 2025

Kabul Tarihi

22 Şubat 2026

Yayımlandığı Sayı

Yıl 2026 Sayı: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1841549

IZ

https://izlik.org/JA46GX55ZW

Kaynak Göster

RIS / Bibtex

APA

Besharatı, M., Farmani, B., & Azhir, D. (2026). Geographical Variations in Physicochemical and Antioxidant Properties of Propolis from Meshgin Apiaries, Iran. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, Advanced Online Publication, 1557-1566. https://doi.org/10.18016/ksutarimdoga.vi.1841549

Geographical Variations in Physicochemical and Antioxidant Properties of Propolis from Meshgin Apiaries, Iran

Abstract

Keywords

Geographical Variations in Physicochemical and Antioxidant Properties of Propolis from Meshgin Apiaries, Iran

Öz

Anahtar Kelimeler

Etik Beyan

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

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