Investigation of Cytotoxic Effect of Salvia pilifera Extracts and Synthetic Chlorogenic and Caffeic Acids on DU145 Prostate Cancer Cells Line
Abstract
Salvia species have been used in the treatment of
many diseases due to their medical effects, and the effects of these species on
prostate cancer cells should be investigated in more detail. In this study, we
aimed to determine anti-carcinogenic activities of dichloromethane (DCM) and
methanol (MeOH) extracts of Salvia
pilifera and the synthetic chloregenic (CGA) and caffeic acids (CA) on
DU-145 prostate cancer cells. The cytotoxicity of extracts and synthetic
compounds on cell viability of DU-145 was measured by using MTT method.
Induction of apoptosis was tested by using Annexin V and 7ADD staining. DNA
fragmentation was evaluated in cells. Also, transcripton levels of Bax, Caspase
3, Caspase 9 and Bcl-2 and Bcl-xL genes were determined. Lastly, the phenolic
compounds in MeOH extract were determined by HPLC. In MTT test, extracts, CGA
and CA were found to be diminished proliferation of DU145 cells. However, in
apoptosis assay, no apoptotic activity for extract and synthetic compounds was
observed. In DNA fragmentation test, while no significant difference in
extracts group was observed as compared to controls, fragmentation as swab in
synthetic compound groups was observed. Small changes were observed in
transcription levels of apoptotic and antipoptotic genes. A total
of 11 phenolic acids were determined including fumaric acid, gallic acid,
gallocatechin, catechin, oleorufein, 4-hydroxybenzoic acid, caffeic acid,
syringic acid, ellagic acid, 3-hydroxy cinnamic acid and protocatechuic acid. Results of the present study suggest that S. pilifera extracts and synthetic CGA
and CA might have cytotoxic effects on DU145 cell at certain concentration (≥50
µg ml-1 for DCM;
≥ 100 µg ml-1 for
MeOH; ≥ 1 µg ml-1 for CGA
and CA) yet that these effects may be manifested through another pathway but
apoptosis.
Keywords
Kaynakça
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Ayrıntılar
Birincil Dil
İngilizce
Konular
-
Bölüm
Araştırma Makalesi
Yazarlar
Mustafa Pehlivan
Celal Güven
İbrahim Bozgeyik
Esra Bozgeyik
Pınar Yumrutaş
Ebru Temiz
Fatih Üçkardeş
Yayımlanma Tarihi
30 Nisan 2018
Gönderilme Tarihi
29 Mart 2017
Kabul Tarihi
22 Mayıs 2007
Yayımlandığı Sayı
Yıl 2018 Cilt: 21 Sayı: 2
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