Mn, Cd, Fe ve Mg Metallerinin Saccharomyces cerevisiae Mayasında Antioksidan Enzim Aktiviteleri Üzerine Etkisi

Oğuz Ayhan Kireçci

doi:10.18016/ksudobil.359165

TR EN

Mn, Cd, Fe ve Mg Metallerinin Saccharomyces cerevisiae Mayasında Antioksidan Enzim Aktiviteleri Üzerine Etkisi

Öz

Metal iyonları, kalıcı etkilerinden dolayı hem canlı sistemler hem de çevre sağlığı yönünden önem taşımakta olup, belirli bir sınırı aşınca da son derece toksik etki gösterirler. Özellikle ağır metaller bu konuda en riskli grubu oluşturur. Bu çalışmada Saccharomyces cerevisiae'da, mangan (Mn), kadmiyum (Cd), demir (Fe) ve magnezyumun (Mg) antioksidan enzimler üzerindeki etkisinin ortaya konması amaçlanmıştır. Deney materyali olan S. cerevisiae FMC16, YEDP besiyerinde çoğaltılmış ve geliştirilmiştir. Uygulama grupları için; Mn, Cd, Fe ve Mg metallerinin her biri son derişimi 100 mL’de 1 mg olacak şekilde kültür ortamına ilave edilmiş ve uygulama yapılan (deneysel grup) ve yapılmayan (kontrol grubu) mayalarda süperoksit dismutaz (SOD), glutatyon S-transferaz (GST) ve glutatyon redüktaz (GSH-Rd) enzim aktiviteleri spektrofometrik yöntemlerle belirlenmiştir. Sonuç olarak; kontrol grubuna göre tüm deneysel gruplarda SOD aktivitesinin arttığı ve bu artışın istatiksel açıdan önemli olduğu ( p<0.001), ve özellikle Cd ile muamele edilen grupta SOD enzim aktitesi artışının daha belirgin olduğu gözlemlenmiştir. GST aktivitesinin uygulama yapılan tüm gruplarda kontrol grubuna göre arttığı (p<0.0001) ve Fe ile muamele edilen mayalarda GST enzim aktivitesindeki artışın diğer gruplara oranla fazla olduğu, GSH-Rd aktivitesinin ise bütün metal uygulama gruplarında kontrole göre azaldığı ve bu azalmanın Cd grubunda oldukça belirgin olduğu saptanmıştır (p<0.0001). Sonuçlar; S. Cerevisae’da farklı metallerin antioksidan savunma sistemi üzerinde farklı etkilere sahip olduğunu göstermiştir.

Anahtar Kelimeler

The Effects of Mn, Mg, Cd, Fe Metals on The Avtivitiy of Antioxidant Enzymes in Saccharomyces cerevisiae

Abstract

Metal ions, despite being important for both live systems and environmental health due to their permanent effects, and may become extremely toxic effect if they exceed a certain limit. Especially heavy metals are considered as the most risky group in this regard. In this study it is intended to reveal the effect of manganese (Mn), cadmium (Cd), iron (Fe) and magnesium (Mg) on antioxidant enzymes in Saccharomyces cerevisiae. S. cerevisiae FMC16, the strain used in this experiments, was proliferated and developed on YEDP medium. For application groups; Mn, Cd, Fe and Mg metals were separately added to the culture medium at the final concentration of 1 mg at 100 mL. Superoxide dismutase (SOD), glutathione S-transferase (GST) and glutathione reductase (GSH-Rd) enzyme activities were determined spectrophotometrically in the treated (experimental group) and non-treated (control group) yeasts. As a result; it was observed that SOD activity was increased and the increase was statistically significant (p <0.001) in all experimental groups compared to the control group altough rise of SOD enzyme activity was more prominent especially in the group treated with Cd. While the activity of GST was found to be higher in all treated groups compared to the control (p <0.0001) and the increase in Fe-treated yeast was observed to be higher than in the other groups, the level of GSH-Rd decreased in all groups compared to the control, and it was especially prominent in the Cd group (p <0.0001). The results showed that, different metals have different effects on the antioxidant defense system in S. cerevisae.

Keywords

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Oğuz Ayhan Kireçci

Yayımlanma Tarihi

31 Ağustos 2018

Gönderilme Tarihi

29 Kasım 2017

Kabul Tarihi

12 Şubat 2018

Yayımlandığı Sayı

Yıl 2018 Cilt: 21 Sayı: 4

DOI

https://doi.org/10.18016/ksudobil.359165

IZ

https://izlik.org/JA62SK47BR

Kaynak Göster

RIS / Bibtex

APA

Kireçci, O. A. (2018). Mn, Cd, Fe ve Mg Metallerinin Saccharomyces cerevisiae Mayasında Antioksidan Enzim Aktiviteleri Üzerine Etkisi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 21(4), 520-528. https://doi.org/10.18016/ksudobil.359165