Phytochemical Profiles and Antiproliferative Effect of Allium tuncelianum

Bu çalışmada, Anadoluda yetişen endemik sarımsak türlerinden biri olan Tunceli Dağ Sarımsağı (Allium tuncelianum) incelenmiştir. Fitokimyasal içeriği belirlemek için LC-MS / MS, HPLC, GC-MS, GC-FID ve ICP-OES teknikleri kullanılmıştır. Allium tuncelianum'un başlıca fenolik bileşenleri; malik asit (3322,6 µg / g), kainik asit (626.8 µg / g), sinamik asit (69.15 µg / g), fumarik asit (13.02 µg / g) ve kateşin (3933.3 µg / g) olduğu belirlendi. Uçucu yağın ana bileşenleri, dialil disülfit (% 28.30), dialil trisülfit (% 30.90) ve allil metil trisülfit (% 9.44) idi. Yağ asidi bileşimi; oleik asit (% 27.19), linoleik asit (% 19.46) ve elaidik asit (% 3) iken, mineral içeriğindeki major elementler; potasyum (4207 ± 67 mg / kg), kalsiyum (518 ± 35 mg / kg) ve magnezyum (376 ± 20) mg / kg) olarak belirlenmiştir. Prostat, kolon, servikal ve meme kanseri hücre dizilerinde sitotoksik etki de incelenmiştir. Kolon, Meme, Servikal ve Prostat kanseri hücre hatlarında sitotoksik etki tespit edildi. Bu sonuçlar göstermektedir ki; Allium tuncelianum, zengin fitokimyasal içeriği ve antikanser aktivitesi ile yeni bir fitoterapi ajanı olabilir.

In this study, Allium tuncelianum, one of the endemic garlic species growing in Anatolia, was investigated for its phytochemical content. LC-MS/MS, HPLC, GC-MS, GC-FID and ICP-OES techniques were used for this purpose. Major phenolic components of Allium tuncelianum were found to be malic acid (3322.6 µg/g), kainic acid (626.8 µg/g), cinnamic acid (69.15 µg/g), fumaric acid (13.02 µg/g) and catechin (3933.3 µg/g). The main components of volatile oil, on the other hand, were detected as diallyl disulfide (28.30%), diallyl trisulfide (30.90%) and allyl methyl trisulfide (9.44%). The fatty acid composition of the plant was revealed as oleic acid (27.19%), linoleic acid (19.46%) and elaidic acid (3%) whereas high content of potassium (4207± 67 mg/kg), calcium (518± 35 mg/kg) and magnesium (376 ± 20 mg/kg) was determined as the minerals. Moreover, cytotoxic effects of ethanol/water and hexane/chloroform extracts of the plant were evaluated in prostate, colon, cervical and breast cancer cell lines and cytotoxicity of both extracts were detected for all the cell lines studied. Therefore, we conclude that Allium tuncelianum may be a new phytotherapy agent with its rich phytochemical content and anticancer activity.