Effects of Melatonin and IAA on Adventitious Root Formation in Rootstock 5BB and cv. Cabernet Sauvignon (Vitis vinifera L.)

Zeliha Gökbayrak; Hakan Engin; Hatice Kiraz

doi:10.18016/ksutarimdoga.vi.672685

TR EN

5BB anacı ve Cabernet Sauvignon (Vitis vinifera L.) Üzüm Çeşidinin Adventif Kök Oluşumu Üzerine Melatonin ve IAA’in Etkileri

Öz

Triptofandan sentezlenen melatonin (MEL) ve indol asetik asit (IAA)’in iki Vitis türünün (5BB anacı ve Cabernet Sauvignon çeşidi) köklenmesi üzerine etkileri açısından denenmiştir. İki gözlü çelikler, bazal kısımları aşağıda belirtilen uygulamalar içine daldırıldıktan sonra iklim odasında yetiştirilmiştir: 0 (kontrol), 5.7, 11.4 ve 16.1 µM konsantrasyona sahip IAA solüsyonunda 5 saniye ve 0 (kontrol), 0.1, 0.5 ve 1.0 µM konsantrasyona sahip melatonin solüsyonlarında 10 dakika. Bulgular her iki türde köklenme, sürme ve sağlıklı bitki yüzdelerinin istatistiksel anlamda uygulamaların önemli etkisi altında olmadığını göstermiştir. Ancak artan IAA konsantrasyonlarının 5BB anacında köklenme yüzdesini de yükselttiği görülmüştür. Diğer yandan 0.1 ve 0.5 µM MEL uygulamaları da çeliklerde köklenmeyi teşvik etmiştir. Cabernet Sauvignon’da 11.4 µM IAA en yüksek yüzdeyi (%100) sağlamıştır. Melatonin köklenmede IAA’ya göre göreceli bir düşüşe neden olmuştur ve Melatoninin köklenme üzerindeki işlevi IAA’dan bağımsız gibi görünmektedir. Hormon uygulamaları 5BB’de çelik başına kök sayısı ve taze kök ağırlığı üzerinde önemli etkilere neden olmuştur. Cabernet Sauvignon’da hormonların etkisi, kök sayısı, kök uzunluğu, kök dağılım şekli ile yaş ve kuru ağırlıkları da dahil olmak üzere köklenme ilgili tüm özellikler üzerine etkili bulunmuştur. Sonuçlar asmada köklenme üzerine Melatoninin etkisini belirlemek için detaylı çalışmaların yapılması gerektiğini ve ucuz olması ve saf halde kolaylıkla elde edilebilmesi nedeniyle Melatoninin çoğaltmada kullanılabilme olasılığı olduğunu göstermektedir.

Anahtar Kelimeler

Köklenme,Indol-3-asetik asit,Melatonin,Vitis

Effects of Melatonin and IAA on Adventitious Root Formation in Rootstock 5BB and cv. Cabernet Sauvignon (Vitis vinifera L.)

Abstract

Both derived from tryptophan, melatonin (MEL) and indole acetic acid (IAA) were tested for their influence on rooting in the cuttings of two Vitis species, the 5BB rootstock and the cultivar Cabernet Sauvignon. The 2-bud cuttings were grown in a growth chamber after the basal ends were dipped in the following treatments; for 5 seconds in IAA solutions at 0 (the control), 5.7, 11.4 and 16.1 µM concentrations, and for 10 minutes in the melatonin solutions at 0 (the control), 0.1, 0.5 and 1.0 µM. The results indicated that percentages of rooting, shooting and healthy plants in both species were not statistically significant under the influence of the treatments. However, increasing concentrations of IAA resulted in increasing rooting percentages in 5BB rootstock. On the other hand, 0.1 and 0.5 µM MEL also induced rooting. In the cuttings of Cabernet Sauvignon, 11.4 µM IAA provided the highest percentage (100%). Melatonin caused a relative decrease in rooting compared to the IAA. The action of melatonin on rooting seems to be independent of IAA. Hormone treatments caused significant differences in the root number per cutting and fresh root weight in 5BB. Effects of the hormones were important on all the rooting features, including root number, root length, root distribution pattern, and weight of fresh and dry roots in Cabernet Sauvignon. Results indicate that extensive research is needed to extrapolate the influence of melatonin on rooting in grapevine with a possibility in practical use since it is inexpensive and easy to obtain in pure form.

Keywords

Rooting,Indole-3-Acetic Acid,Melatonin,Vitis

Destekleyen Kurum

Çanakkale Onsekiz Mart Üniversitesi BAP Başkanlığı

Proje Numarası

FBA-2018-1392

Teşekkür

This work was supported by Çanakkale Onsekiz Mart University The Scientific Research Coordination Unit, Project number: FBA-2018-1392. This work is also a part of Master’s thesis conducted by H. Kiraz under the supervision of first author. It was presented at 1st International Eurasian Conference on Science, Engineering and Technology (EurasianSciEnTech 2018) in Ankara, TURKEY on November 22-23, 2018, and was published in the abstract book.

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Ziraat, Veterinerlik ve Gıda Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Zeliha Gökbayrak ^*
0000-0002-0012-9782
Türkiye

Hakan Engin
0000-0001-6897-8708
Türkiye

Hatice Kiraz
0000-0003-3070-1273
Türkiye

Yayımlanma Tarihi

31 Ağustos 2020

Gönderilme Tarihi

9 Ocak 2020

Kabul Tarihi

17 Nisan 2020

Yayımlandığı Sayı

Yıl 2020 Cilt: 23 Sayı: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.672685

IZ

https://izlik.org/JA45UU55EN

Kaynak Göster

RIS / Bibtex

APA

Gökbayrak, Z., Engin, H., & Kiraz, H. (2020). Effects of Melatonin and IAA on Adventitious Root Formation in Rootstock 5BB and cv. Cabernet Sauvignon (Vitis vinifera L.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23(4), 835-841. https://doi.org/10.18016/ksutarimdoga.vi.672685