Selective Cytotoxic Effect of Astaxanthin on Human Lung and Colon Cancer Cells

Selim Demir; Elif Ayazoglu Demir; Yüksel Aliyazıcıoğlu

doi:10.18016/ksutarimdoga.vi.712905

TR EN

Astaksantinin İnsan Akciğer ve Kolon Kanseri Hücrelerindeki Seçici Sitotoksik Etkisi

Öz

Astaksantin (ASX), çeşitli mikroorganizmalarda ve deniz hayvanlarında bulunan kırmızı renkli bir ksantofil karotenoididir. ASX, mevcut karotenoidler arasında en yüksek antioksidan aktiviteye sahip olduğundan "süper antioksidan" olarak da adlandırılmaktadır. ASX’in antioksidan özelliğinin yanında, antimikrobiyal, immünomodülatör, hepatoprotektif, antikanser ve antidiyabetik özellikleri de yapılan çalışmalarla gösterilmiştir. Bununla birlikte, ASX'in kanser hücreleri üzerindeki seçici sitotoksik etkilerini inceleyen çalışmalar sınırlıdır. Bu çalışmanın amacı, ASX'in yaygın kanser türlerini temsil eden hücreler üzerindeki sitotoksik etkilerini belirlemektir. Bunun için insan meme (MCF-7), akciğer (A549), karaciğer (HepG2), melanoma (VMM917), kolon (WiDr) kanseri ve normal fibroblast hücreleri 72 saat boyunca farklı konsantrasyonlarda ASX ile muamele edildi ve ardından MTT protokolü uygulandı. Cisplatin sitotoksisite deneylerinde pozitif kontrol olarak kullanıldı. Sonuçlar, ASX'in incelenen tüm kanser hücre hatları üzerinde doza bağımlı bir sitotoksik etkiye sahip olduğunu gösterdi. Bununla birlikte, fibroblast hücreleri ile kıyaslandığından ASX'in en güçlü seçici sitotoksik etkisinin A549 ve WiDr hücrelerinde olduğu belirlendi. Bu çalışma ASX'in seçici sitotoksik etkisinin, özellikle akciğer ve kolon kanseri açısından daha kapsamlı şekilde araştırılması gerektiğini göstermektedir.

Anahtar Kelimeler

Astaksantin,Hücre kültürü,Kanser,Sitotoksisite

Selective Cytotoxic Effect of Astaxanthin on Human Lung and Colon Cancer Cells

Abstract

Astaxanthin (ASX) is a red xanthophyll carotenoid found in various microorganisms and marine animals. ASX is also called the "super antioxidant" because it has the highest antioxidant activity among existing carotenoids. Studies have shown not only antioxidant properties but also antimicrobial, immunomodulatory, hepatoprotective, anticancer and antidiabetic properties of ASX. However, there is a limited number of studies examining the selective cytotoxic effects of ASX on cancer cells. The aim of this study was to determine the cytotoxic effects of ASX on cells representing common cancer types. For this, human breast (MCF-7), lung (A549), liver (HepG2), melanoma (VMM917), colon (WiDr) cancer and normal fibroblast cells were treated with different concentrations of ASX for 72 h and then the MTT assay protocol was applied. Cisplatin was used as a positive control in cytotoxicity experiments. The results showed that ASX had a dose-dependent cytotoxic effect on all studied cancer cell lines. However, the strongest selective cytotoxic effect of ASX was determined in A549 and WiDr cells compared to fibroblast cells. This study shows that selective cytotoxic effect of ASX should be investigated more extensively, especially in terms of lung and colon cancer.

Keywords

Astaxanthin,Cancer,Cell culture,Cytotoxicity

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Selim Demir ^*
0000-0002-1863-6280
Türkiye

Elif Ayazoglu Demir
0000-0001-9027-7633
Türkiye

Yüksel Aliyazıcıoğlu
0000-0001-9474-4307
Türkiye

Yayımlanma Tarihi

31 Aralık 2020

Gönderilme Tarihi

1 Nisan 2020

Kabul Tarihi

14 Mayıs 2020

Yayımlandığı Sayı

Yıl 2020 Cilt: 23 Sayı: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.712905

IZ

https://izlik.org/JA36CM84XL

Kaynak Göster

RIS / Bibtex

APA

Demir, S., Ayazoglu Demir, E., & Aliyazıcıoğlu, Y. (2020). Selective Cytotoxic Effect of Astaxanthin on Human Lung and Colon Cancer Cells. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23(6), 1489-1494. https://doi.org/10.18016/ksutarimdoga.vi.712905

Investigation of Growth Properties of BHK-21 Cells with Different Characteristics to Comprise a Cell Bank in Low Serum-Supplement Medium in The Monolayer Culture System

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

https://doi.org/10.18016/ksutarimdoga.vi.1759236

Selective Cytotoxic Effect of Astaxanthin on Human Lung and Colon Cancer Cells

Astaksantinin İnsan Akciğer ve Kolon Kanseri Hücrelerindeki Seçici Sitotoksik Etkisi

Öz

Anahtar Kelimeler

Selective Cytotoxic Effect of Astaxanthin on Human Lung and Colon Cancer Cells

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

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Investigation of Growth Properties of BHK-21 Cells with Different Characteristics to Comprise a Cell Bank in Low Serum-Supplement Medium in The Monolayer Culture System

e-ISSN: 2619-9149