Evaluation of Cytotoxic Effect of Onosma armeniacum Extract on Various Cancer Cells

Selim Demir; Elif Ayazoglu Demir; İbrahim Turan; Ufuk Özgen

doi:10.18016/ksutarimdoga.vi.729814

TR EN

Onosma armeniacum Ekstraktının Çeşitli Kanser Hücrelerindeki Sitotoksik Etkisinin Değerlendirilmesi

Öz

Bu çalışmanın amacı O. armeniacum kök ekstraktının antioksidan özellikleri ile normal bir fibroblast hücre hattına kıyasla insan karaciğer (HepG2), akciğer (A549) ve kolon (WiDr) kanser hücre hatlarındaki sitotoksik etkisini araştırmaktır. Ekstraktın antioksidan özellikleri kolorimetrik yöntemler kullanılarak belirlenmiştir. Ekstraktın sitotoksik etkisi MTT yöntemi kullanılarak araştırılmıştır. Ekstraktın toplam fenolik içerik ve indirgeyici güç değerleri, g örnek başına sırasıyla 26.1±0.4 mg gallik asit eşdeğeri ve 52.6±1.1 mg troloks eşdeğeri olarak hesaplanmıştır. Ekstrakt, çalışılan tüm kanser hücre serilerinde, özellikle HepG2 (3.6 kat), seçici sitotoksik etki göstermiştir. Çalışmanın sonuçları, kabul edilebilir antioksidan ve sitotoksik etkilere sahip olduğu saptanan O. armeniacum'un daha kapsamlı çalışmalarla incelenmesi gerektiğini ortaya koymaktadır.

Anahtar Kelimeler

Evaluation of Cytotoxic Effect of Onosma armeniacum Extract on Various Cancer Cells

Abstract

The aim of this study was to investigate the antioxidant properties of the root extract of Onosma armeniacum and its cytotoxic effect against human liver (HepG2), lung (A549) and colon (WiDr) cancer cell lines compared with a normal fibroblast cell line. Antioxidant properties of the extract was determined using the colorimetric methods. The cytotoxic effect of the extract were investigated using MTT assay. The total phenolic content and reducing power values of extract were 26.1±0.4 mg gallic acid equivalent and 52.6±1.1 mg trolox equivalent per g sample, respectively. The extract exhibited selective cytotoxic effect in all studied cancer cell lines, especially HepG2 (3.6-fold). The results of this study reveal that O. armeniacum, which has been found to have acceptable antioxidant and cytotoxic effects, should be examined with more comprehensive studies.

Keywords

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Selim Demir ^*
0000-0002-1863-6280
Türkiye

Elif Ayazoglu Demir
0000-0001-9027-7633
Türkiye

İbrahim Turan
0000-0003-3400-5494
Türkiye

Ufuk Özgen
0000-0001-9839-6717
Türkiye

Yayımlanma Tarihi

30 Nisan 2021

Gönderilme Tarihi

30 Nisan 2020

Kabul Tarihi

18 Haziran 2020

Yayımlandığı Sayı

Yıl 2021 Cilt: 24 Sayı: 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.729814

IZ

https://izlik.org/JA83GU74ZK

Kaynak Göster

RIS / Bibtex

APA

Demir, S., Ayazoglu Demir, E., Turan, İ., & Özgen, U. (2021). Evaluation of Cytotoxic Effect of Onosma armeniacum Extract on Various Cancer Cells. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 24(2), 252-259. https://doi.org/10.18016/ksutarimdoga.vi.729814