The Effect of Some Parameters on the Production of L(+) Lactic Acid Using Wheat wastewater by Rhizopus oryzae NRRL-395

Ali Göçeri; Mehmet Hakkı Alma; Yekta Gezginc; Eyyüp Karaoğul

doi:10.18016/ksutarimdoga.vi.739225

EN TR

The Effect of Some Parameters on the Production of L(+) Lactic Acid Using Wheat wastewater by Rhizopus oryzae NRRL-395

Abstract

The aim of the study was to investigate some major parameters on production lactic acid using wheat wastewater by Rhizopus oryzae NRRL-395. The parameters, which may play an effective role in production, were determined. For developing and producing pure L(+)-Lactic acid of the filamentous fungus Rhizopus oryzae NRRL-395, rich medium with wheat wastewater and glucose as carbon source were used in the study. The effects of carbon sources concentration, pH, agitation (shaking speed) and spores concentration on lactic acid production were examined. After fermentation process, the highest values of lactic acid obtained from wheat wastewater and glucose were analyzed using high-performance liquid chromatography with ultraviolet detectors (HPLC-UV). The maximum L (+) Lactic acid production in shake flasks was investigated at pH 6, 1.0x106 spores/mL, 150 rpm, 30 ̊C at 8 days. The maximum lactic acid content for wheat wastewater and glucose were obtained at a concentration of 100% (5.638 g/L) and 150 g/L (5.042 g/L). The maximum lactic acid amount of 5.568 g/L was obtained at pH value of 6 for wheat wastewater. However, the maximum lactic acid amount of 2.463 g/L was obtained at pH value of 6 for glucose. The maximum lactic acid values for wheat wastewater and glucose were obtained 5.603 g/L and 2.483 g/L at 150 rpm speed, respectively. The maximum lactic acid values for wheat wastewater and glucose were obtained 5.804 g/L and 5.646 g/L at the 1.0x106 spores/mL respectively.

Keywords

Atık Bulgur Suyu Kullanılarak Rhizopus oryzae NRRL-395 ile Üretilen Laktik Asite Bazı Parametrelerin Etkisi

Öz

Bu çalışmanın amacı, Rhizopus oryzae NRRL-395 tarafından bulgur atık suyu kullanılarak üretilen laktik asit üretimine bazı önemli parametrelerin etkisi denenmiştir. Bu amaç ile filamentli bir yapıya sahip olan Rhizopus oryzae fungusunun gelişimi ve laktik asit üretimi için bulgur atık suyu ve glukoz gibi karbon kaynakları bu çalışmada kullanılmıştır. Karbon kaynaklarının konsantrasyonunun, pH’larının, çalkalama hızlarının ve spor konsantrasyonlarının üretim üzerine olan etkileri incelenmiştir. Fermentasyon işleminden sonra, bulgur atık suyu ve glukozdan elde edilen yüksek laktik asit değerleri yüksek performanslı sıvı kromatografisi (HPLC-UV) kullanılarak analiz edilmiştir. Çalkalamalı sistemde en yüksek laktik asit değerleri için pH 6, 1.0x106 spor/mL konsantrasyonunda, 150 rpm hızında ve 30 ̊C de 8 gün olarak araştırılmıştır. Bulgur atık suyu için maksimum laktik asit miktarı %100 konsantrasyonda (5.638 g/L) ve glukoz için 150 g/L konsantrasyonda (5.042 g/L) olarak elde edilmiştir. Bulgur atık suyu ve glukoz için maksimum laktik asit oranları sırası ile pH 6’da 5.568 ve 2.463 g/L, 150 rpm hızında 5.603 ve 2.483 g/L ve 1.0x106 spor/mL konsantrasyonunda ise 5.804 ve 5.646 g/L olarak elde edilmiştir.

Anahtar Kelimeler

Destekleyen Kurum

Kahramanmaraş Sütçü İmam Üniversitesi Bilimsel Araştırma Projeleri Yönetim Birimi Başkanlığı tarafından desteklenmiştir.

Proje Numarası

20133-4YLS

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Ziraat, Veterinerlik ve Gıda Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Ali Göçeri ^*
0000-0002-1218-6696
Türkiye

Mehmet Hakkı Alma
0000-0001-7011-3965
Türkiye

Yekta Gezginc
0000-0002-3230-2850
Türkiye

Eyyüp Karaoğul
0000-0001-8162-6838
Türkiye

Yayımlanma Tarihi

30 Nisan 2021

Gönderilme Tarihi

18 Mayıs 2020

Kabul Tarihi

18 Temmuz 2020

Yayımlandığı Sayı

Yıl 2021 Cilt: 24 Sayı: 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.739225

IZ

https://izlik.org/JA39UY53EA

Kaynak Göster

RIS / Bibtex

APA

Göçeri, A., Alma, M. H., Gezginc, Y., & Karaoğul, E. (2021). The Effect of Some Parameters on the Production of L(+) Lactic Acid Using Wheat wastewater by Rhizopus oryzae NRRL-395. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 24(2), 293-298. https://doi.org/10.18016/ksutarimdoga.vi.739225