Burdur İlinde Plum pox virus’un Tespiti ve Kısmi Kılıf Protein Geninin Moleküler Karakterizasyonu

Ali Ferhan Morca; Sevgi Coşkan; Ali Çelık

doi:10.18016/ksutarimdoga.vi.767409

EN TR

Determination of Plum pox virus and Molecular Characterization of Partial Coat Protein Gene in Burdur Province

Abstract

Plum pox virus (PPV), the causal agent of Sharka disease, causes significant yield losses in stone fruits. Despite PPV occurrence as restricted in different regions of Turkey, there is no record of PPV yet in Burdur province. Hence, this study includes the results for determining the presence of PPV in Burdur province between 2016-2019 by serological and molecular methods. A total of 47 leaf samples collected during the surveys were first analyzed by Double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), and only 2-peach samples were determined to be infected with PPV. A degenerate primer pair that amplifies a 767 bp product was designed for the partial coat protein region of PPV in this study. Reverse transcription-polymerase chain reaction (RT-PCR) was performed using designed primer pair, and novel isolates obtained by sequence analysis were deposited in the GenBank. As a result of the BlastN analysis, Burdur PPV isolates showed the highest similarity (99.86% -98.49%) with the PPV-M strain. The phylogenetic tree was generated by the Neighbor-Joining method, resulting in novel PPV-Burdur isolates were clustered with the PPV-M isolates belonging to Turkey and other countries. The novel two PPV-M isolates were the first records in the province of Burdur, the PPV-free area in the Mediterranean Region. As a result of this study, by eradicating infected trees, a one-kilometer diameter buffer zone was established. It has been decided to ban the replanting of Prunus species and continue the survey studies for three years.

Keywords

Burdur İlinde Plum pox virus’un Tespiti ve Kısmi Kılıf Protein Geninin Moleküler Karakterizasyonu

Öz

Plum pox virus (PPV), sert çekirdekli meyvelerde önemli verim kayıplarına sebep olan Şarka hastalığının etmenidir. PPV, Türkiye’nin farklı bölgelerinde sınırlı olarak tespit edilmesine rağmen, bugüne kadar Burdur iline ait herhangi bir kayıt bulunmamaktadır. Bu çalışmada, 2016-2019 yılları arasında Burdur ilinde PPV’nin varlığının belirlenmesine yönelik serolojik ve moleküler yöntemler kullanılarak yapılan araştırmanın sonuçlarına yer verilmiştir. Sürveyler süresince toplanan 47 adet sert çekirdekli meyve yaprak örneği ilk olarak Double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) ile analiz edilmiş ve 2 adet şeftali örneğinin PPV ile enfekteli olduğu belirlenmiştir. Çalışmada, PPV’nin kılıf protein bölgesi için 767 nükleotid büyüklüğünde bir bölgeyi çoğaltan bir adet dejenere primer çifti tasarlanmıştır. Yeni primerler ile gerçekleştirilen Reverse transcription-polymerase chain reaction (RT-PCR) ve sekans analizleri sonucunda elde edilen diziler GenBank’a kaydedilmiştir. Yapılan BlastN analizi neticesinde Burdur PPV izolatları en yüksek benzerlik oranını (%99.86-%98.49), PPV-M ırkı ile göstermiştir. Neighbour-joining yöntemiyle yapılan filogenetik ağaçta Burdur izolatlarının, Türkiye ve farklı ülkelere ait PPV-M izolatı ile kümelendiği belirlenmiştir. Bu çalışma ile elde edilen 2 adet PPV-M izolatı Akdeniz Bölgesi’nin PPV açısından ari alanı konumunda olan Burdur ilinde ilk kayıt niteliğindedir. Çalışma sonucunda enfekteli olduğu tespit edilen ağaçlar eradike edilerek 1 km çapında tampon bölge oluşturulmuş ve 3 yıl boyunca Prunus türlerinin yetiştiriciliğinin yasaklanmasına ve sürvey çalışmalarınnın devam edilmesine karar verilmiştir.

Anahtar Kelimeler

Destekleyen Kurum

Tarım ve Orman Bakanlığı

Teşekkür

Bu çalışmada sürvey çalışmalarında emeği geçen Tarım ve Orman Bakanlığı Burdur İl Tarım ve Orman Müdürlüğünde görevli personelleri ile Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü Zirai Mücadele Merkez Araştırma Enstitüsü Müdürlüğüne desteklerinden dolayı teşekkür ediyoruz.

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Ziraat, Veterinerlik ve Gıda Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Ali Ferhan Morca ^*
0000-0002-7480-922X
Türkiye

Sevgi Coşkan
0000-0002-3589-6041
Türkiye

Ali Çelık
0000-0002-5836-8030
Türkiye

Yayımlanma Tarihi

31 Ağustos 2021

Gönderilme Tarihi

10 Temmuz 2020

Kabul Tarihi

4 Aralık 2020

Yayımlandığı Sayı

Yıl 2021 Cilt: 24 Sayı: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.767409

IZ

https://izlik.org/JA99AS52ZP

Kaynak Göster

RIS / Bibtex

APA

Morca, A. F., Coşkan, S., & Çelık, A. (2021). Burdur İlinde Plum pox virus’un Tespiti ve Kısmi Kılıf Protein Geninin Moleküler Karakterizasyonu. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 24(4), 805-814. https://doi.org/10.18016/ksutarimdoga.vi.767409

The monitoring of plum pox virus in Bursa, Bilecik, and Bolu provinces of Turkey refined MIs status as a variant of strain M

Archives of Phytopathology and Plant Protection

https://doi.org/10.1080/03235408.2022.2052523

Strengthening hazelnut certification systems through sensitive detection of apple mosaic virus (ApMV)

Journal of Plant Diseases and Protection

https://doi.org/10.1007/s41348-025-01098-x

Marmara bölgesinde sert çekirdekli meyve ağaçlarında şarka hastalığının (plum pox virus=PPV) tespiti ve moleküler karakterizasyonu

Harran Tarım ve Gıda Bilimleri Dergisi

https://doi.org/10.29050/harranziraat.1643402

Performance of Ready-To-Use Isothermal Detection Kits in Plum pox virus (PPV) Detection

Türk Tarım ve Doğa Bilimleri Dergisi

https://doi.org/10.30910/turkjans.1665625

Burdur İlinde Plum pox virus’un Tespiti ve Kısmi Kılıf Protein Geninin Moleküler Karakterizasyonu

Determination of Plum pox virus and Molecular Characterization of Partial Coat Protein Gene in Burdur Province

Abstract

Keywords

Burdur İlinde Plum pox virus’un Tespiti ve Kısmi Kılıf Protein Geninin Moleküler Karakterizasyonu

Öz

Anahtar Kelimeler

Destekleyen Kurum

Teşekkür

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

Comprehensive surveillance and population study on plum pox virus in Ankara Province of Turkey

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High genetic diversity suggests a long evolutionary history of plum pox virus‐M in Turkey

The monitoring of plum pox virus in Bursa, Bilecik, and Bolu provinces of Turkey refined MIs status as a variant of strain M

Strengthening hazelnut certification systems through sensitive detection of apple mosaic virus (ApMV)

Marmara bölgesinde sert çekirdekli meyve ağaçlarında şarka hastalığının (plum pox virus=PPV) tespiti ve moleküler karakterizasyonu

Performance of Ready-To-Use Isothermal Detection Kits in Plum pox virus (PPV) Detection

e-ISSN: 2619-9149