Geleneksel Yöntem ve Direkt Fermantasyon ile Üretilen Maraş Tarhanalarında Biyoaktif Peptitlerin Belirlenmesi

Doğanay Yüksel; Ahmet Levent İnanç

doi:10.18016/ksutarimdoga.vi.887719

EN TR

Determination of Bioactive Peptides in Maras Tarhana Produced by Traditional Method and Direct Fermentation

Abstract

In this study, two different techniques namely traditional and direct fermentation method were used to produce Maraş tarhana. Peptide profiles of tarhana samples were compared by RP-HPLC method, and investigated by Q-TOF LC/MS. Mass spectrometry data were analyzed in PEAKS Studio (version 8.5) software and the physiological activities of the peptides were investigated in BIOPEP database. According to MS data, 34 and 50 different peptides were determined in the traditional and direct fermentation tarhana hydrolyzates, respectively. Among them, a total of 8 ACE inhibitor peptides, 3 antioxidant peptides, 1 antibacterial peptide, 4 DPP-IV inhibitor peptides, 1 renin inhibitor peptide were found in the BIOPEP database. 19 different peptide were detected in the yogurt hydrolyzate. Two of them (VLPVPQK, EAMAPK) were also detected in tarhana hydrolyzates. Overall, 23 peptides were detected in wheat samples. One of which (LVVPPK) was also detected in the direct fermented tarhana hydrolyzate. However no common peptide was detected in yoghurt and wheat hydrolyzates. As a result of the literature review, it was found that peptide EAMAPK, which is not included in the BIOPEP database, has antioxidant activity and the peptide IIVTQTMK has the potential to show ACE inhibitory activity. In addition it has been concluded that LPAVFK, LPGLKF, LLSLGA, and LVVPPK peptides sustained the potential to show antimicrobial activity considering the amino acid sequence and types.

Keywords

Geleneksel Yöntem ve Direkt Fermantasyon ile Üretilen Maraş Tarhanalarında Biyoaktif Peptitlerin Belirlenmesi

Öz

Bu çalışmada geleneksel yöntem ve direkt fermantasyon yöntemi olmak üzere iki farklı teknikle üretilen Maraş tarhanaları ve tarhanaların ana bileşenlerinin (yoğurt ve dövme) pepsin-tripsin hidrolizatlarında bulunan 3kDa altındaki kısa zincirli “biyoaktif peptitler” araştırılmıştır. Q-TOF LC/MS cihazından alınan MS verileri PEAKS Studio (versiyon 8.5) programında değerlendirilmiş ve tespit edilen peptitlerin BIOPEP veri setinde fizyolojik aktiviteleri araştırılmıştır. RP-HPLC’de tarhana örneklerinin peptit profilleri kıyaslanmıştır. Geleneksel tarhana hidrolizatlarında 3 kDa altında 34 tane, direkt fermente tarhana hidrolizatlarında ise 50 tane peptit tespit edilmiştir. Bunların 8 tanesi ACE inhibitör peptit, 3 tanesi antioksidan peptit, 1 tanesi antibakteriyal peptit, 4 tanesi DPP IV inhibitör peptit ve 1 tanesi renin inhibitör peptit olarak BIOPEP veri setinde tanımlıdır. Yoğurt hidrolizatlarında tespit edilen 19 peptit zincirinden iki tanesi (VLPVPQK, EAMAPK) tarhana örnekleri ile ortaktır. Dövme hidrolizatında 23 adet peptit tespit edilmiştir. Bunların içinden LVVPPK peptiti direkt fermente tarhana hidrolizatında da tespit edilmiştir. Yoğurt ve dövme hidrolizatlarında ortak peptit bulunmamıştır. BIOPEP veri setinde yer almayan EAMAPK peptitinin antioksidan, IIVTQTMK peptitinin ACE inhibitör ve LPAVFK, LPGLKF, LLSLGA, LVVPPK peptitlerinin amino asit dizilimi ve türleri göz önüne alındığında antimikrobiyal aktivite taşıma kapasiteleri olduğu değerlendirilmiştir.

Anahtar Kelimeler

Destekleyen Kurum

Kahramanmaraş Sütçü İmam Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

2017 / 7-162 YLS

Teşekkür

Bu çalışma, Kahramanmaraş Sütçü İmam Üniversitesi Bilimsel Araştırma Projeleri kapsamında desteklenmiştir (Proje Numarası: 2017 / 7-162 YLS).

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Doğanay Yüksel ^*
0000-0002-1926-5573
Türkiye

Ahmet Levent İnanç
0000-0002-7363-5096
Türkiye

Yayımlanma Tarihi

30 Nisan 2022

Gönderilme Tarihi

27 Şubat 2021

Kabul Tarihi

17 Haziran 2021

Yayımlandığı Sayı

Yıl 2022 Cilt: 25 Sayı: 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.887719

IZ

https://izlik.org/JA34KZ85ZH

Kaynak Göster

RIS / Bibtex

APA

Yüksel, D., & İnanç, A. L. (2022). Geleneksel Yöntem ve Direkt Fermantasyon ile Üretilen Maraş Tarhanalarında Biyoaktif Peptitlerin Belirlenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(2), 357-366. https://doi.org/10.18016/ksutarimdoga.vi.887719