Evaluation of Anticholinergic, Antidiabetic and Antioxidant Activity of Astragalus dumanii, an Endemic Plant

Ümit Muhammet Koçyiğit; Nuraniye Eruygur; Mehmet Ataş; Mehmet Tekin; Parham Taslimi; Faik Gökalp; İlhami Gülçin

doi:10.18016/ksutarimdoga.vi.895616

EN TR

Evaluation of Anticholinergic, Antidiabetic and Antioxidant Activity of Astragalus dumanii, an Endemic Plant

Abstract

The research was conducted to separately evaluate and detect the possible in vitro antioxidant, antimicrobial activity of ethanol extracts prepared from aerial parts and roots of Astragalus dumanii and anti-cholinesterase and α-glucosidase inhibitory activity from only aerial parts of its The antioxidant capacity was tested by scavenging of DPPH and ABTS free radicals. Compared with the standard antioxidant compound gallic acid; Root and aerial part extract showed lower DPPH radical scavenging activity, however aerial part extract demonstrated higher ABTS radical scavenging activity. The phenolic contents were detected as 5.31 ± 0.03 and 13.23 ± 0.05 mg gallic acid equivalent g-1 extract, flavonoid contents were found as 8.26 ± 0.004 and 7.93 ± 0.005 mg Qercetin equivalent g-1 extract. In addition, the effects of the extracts obtained from aerial parts of the plant on acetylcholinesterase, butyrylcholinesterase and a-glycosidase enzymes were investigated in vitro and IC50 values were obtained as 1.47, 0.83 and 0.48 µg mL-1, respectively. When these values were compared with standard substances, it was seen that Astragalus dumanii could be a good enzyme inhibitory agent. Antimicrobial activity of the plant extracts were determined using the microdilution method and the extracts was not observed to have any antimicrobial activities..

Keywords

Sivas da Yetişen Endemik Bir Bitki Olan Astragalus Dumanii'nin Antikolinerjik, Antidiyabetik ve Antioksidan Aktivitesinin Değerlendirilmesi

Öz

Araştırma, Astragalus dumanii bitkisinin topraküstü ve köklerinden hazırlanan etanol ekstrelerinin olası in vitro antioksidan, antimikrobiyal ve yalnızca topraküstü etanol ekstrelerinin anti-kolinesteraz ve α-glikozidaz inhibitör aktivitesini ayrı ayrı değerlendirmek ve tespit etmek için yapılmıştır. Antioksidan kapasitesi, DPPH ve ABTS serbest radikalleri temizleme metoduyla test edildi. Standart antioksidan bileşik gallik asit ile karşılaştırıldığında; Kök ve toprak üstü kısım ekstreleri düşük DPPH radikal süpürücü aktivite gösterirken, toprak üstü kısmı daha yüksek ABTS radikal süpürücü aktivite göstermiştir. Fenolik içerikleri 5,31 ± 0,03 ve 13,23 ± 0,05 mg gallik asite eşdeğer g-1 ekstre, flavonoit içerikleri ise 8,26 ± 0,004 ve 7,93 ± 0,005 mg kersetine eşdeğer g-1 özüt olarak bulunmuştur. Ayrıca bitkinin toprak üstü kısımlarından elde edilen ekstrelerin asetilkolinesteraz, butirilkolinesteraz ve a-glikosidaz enzimlerine etkisi in vitro araştırıldı ve sırasıyla IC50 değerleri: 1.47, 0.83 ve 0.48 µg mL-1 elde edildi. Bu değerler stadart maddelerle karşılaştırıldığında da Astragalus dumanii’nin iyi bir enzim inhibe edici ajan olabileceği görülmüştür. Bitki ekstrelerinin antimikrobiyal aktiviteleri mikrodilüsyon yöntemi kullanılarak belirlendi ve ekstrelerde herhangi bir antimikrobiyal aktivite gözlemlenmedi.

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Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Ümit Muhammet Koçyiğit
0000-0001-8710-2912
Türkiye

Nuraniye Eruygur
0000-0002-4674-7009
Türkiye

Mehmet Ataş
0000-0002-9425-0080
Türkiye

Mehmet Tekin
0000-0002-1157-1314
Türkiye

Parham Taslimi
0000-0002-3171-0633
Türkiye

Faik Gökalp ^*
0000-0003-4363-3839
Türkiye

İlhami Gülçin
0000-0001-5993-1668
Türkiye

Yayımlanma Tarihi

30 Aralık 2022

Gönderilme Tarihi

12 Mart 2021

Kabul Tarihi

19 Kasım 2021

Yayımlandığı Sayı

Yıl 2022 Cilt: 25 Sayı: Ek Sayı 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.895616

IZ

https://izlik.org/JA67CD36BX

Kaynak Göster

RIS / Bibtex

APA

Koçyiğit, Ü. M., Eruygur, N., Ataş, M., Tekin, M., Taslimi, P., Gökalp, F., & Gülçin, İ. (2022). Evaluation of Anticholinergic, Antidiabetic and Antioxidant Activity of Astragalus dumanii, an Endemic Plant. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(Ek Sayı 1), 1-10. https://doi.org/10.18016/ksutarimdoga.vi.895616

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Evaluation of Anticholinergic, Antidiabetic and Antioxidant Activity of Astragalus dumanii, an Endemic Plant

Abstract

Keywords

Sivas da Yetişen Endemik Bir Bitki Olan Astragalus Dumanii'nin Antikolinerjik, Antidiyabetik ve Antioksidan Aktivitesinin Değerlendirilmesi

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

Screening of Antiglaucoma, Antidiabetic, Anti-Alzheimer, and Antioxidant Activities of Astragalus alopecurus Pall—Analysis of Phenolics Profiles by LC-MS/MS

Determination of metabolic profiling by LC-MS/MS, evaluation of antioxidant activities, and enzyme inhibition abilities of Helichrysum plicatum subsp. pseudopliacatum

Anti-Cholinergic Effects of the Phenolic Extract from the Astragalus crenatus Plant: A Computational and Network Pharmacology Study

Evaluation of the in vitro enzyme inhibition and antioxidant activity of Clinopodium betulifolium (Boiss. & Balansa) Kuntze

Unveiling the Phytochemical Diversity and Bioactivity of Astragalus melanophrurius: A First Report Integrating Experimental and In Silico Approaches

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e-ISSN: 2619-9149