UV-C’nin, Deinonoccus radiodurans ve Vitreoscilla Hemoglobin (vgb) Geni Aktarılmış Rekombinantlarında; SOD, KAT ve Karoten Miktarı Üzerine Etkisi

Elif Özbey; Dilek Asma

doi:10.18016/ksutarimdoga.vi.916575

EN TR

In UV-C, Deinonoccus radiodurans and Vitreoscilla Hemoglobin (vgb) Gene Transferred Recombinants; Effect on SOD, KAT and Carotene Amount

Abstract

UV radiation creates oxidative stress by causing the formation of reactive oxygen species in biological tissues. Reactive oxygen species induced by UV, their effects and cellular defense mechanisms against them and antioxidant systems responsible for cleaning reactive oxygen species are the subjects on which much research has been done today. In this study, Deinococcus radiodrans, a well-known extremophile with high levels of ionizing radiation and UV radiation, resistance to many agents and conditions such as drought and DNA damaging chemicals, and Vitreoscilla hemoglobin (vgb) gene cloned recombinant and vgb¯ recombinant strain as a control. used. The effect of UV-C on D. radiodurans' antioxidant defense systems (superoxide dismutase, catalase and carotene) was investigated, and in addition, the contribution of the vgb gene to the UV resistance of the bacteria by providing a more oxygenated environment was investigated. Accordingly, when we compared the UV-C applied samples of D. radiodurans (vgb¯) with the control groups, it was found that superoxide dismutase and catalase enzyme activity was lower than the recombinant carrying wild and vgb genes. Again, in wild-type bacteria with high carotene content, an increase in the amount of carotene was clearly observed due to UV-C application.

Keywords

UV-C’nin, Deinonoccus radiodurans ve Vitreoscilla Hemoglobin (vgb) Geni Aktarılmış Rekombinantlarında; SOD, KAT ve Karoten Miktarı Üzerine Etkisi

Öz

UV radyasyonu, biyolojik dokularda reaktif oksijen türlerinin meydana gelmesine neden olarak oksidatif stres oluşturmaktadır. UV’ nin indüklediği reaktif oksijen türleri, bunların etkileri ve bunlara karşı hücresel savunma mekanizmaları ve reaktif oksijen türlerinin temizlenmesinden sorumlu antioksidan sistemleri günümüzde üzerinde oldukça fazla araştırma yapılan konulardır. Bu çalışmada, yüksek seviyede iyonize radyasyon ve UV radyasyon, kuraklık ve DNA’ ya zarar veren kimyasallar gibi birçok ajan ve koşula olan direnciyle iyi bilinen bir ekstremofil olan Deinococcus radiodrans ile Vitreoscilla hemoglobin (vgb) geni klonlanmış rekombinantı ve kontrol olarak da vgb¯ rekombinant suşu kullanılmıştır. UV-C’ nin D. radiodurans' ın antioksidan savunma sistemleri (süperoksit dismutaz, katalaz ve karoten) üzerine etkisi araştırılıp, buna ek olarak organizmaya daha fazla oksijenli ortam sağlayarak daha fazla büyümesini sağlayan vgb geninin, bakterinin UV direncine yapacağı katkısı araştırılmıştır. Buna göre, D. radiodurans (vgb¯)' in UV-C uygulanan örneklerini kontrol gruplarıyla kıyasladığımızda süperoksit dismutaz ve katalaz enzim aktivitesinin yabanıl ve vgb genini taşıyan rekombinantına oranla daha düşük olduğu tespit edilmiştir. Yine yüksek karoten içeren yabanıl tipi bakterilerde, UV-C uygulamasına bağlı olarak karoten miktar artışı net bir şekilde gözlenmiştir.

Anahtar Kelimeler

Destekleyen Kurum

İnönü Üniversitesi

Proje Numarası

2012-192 nolu BAP projesi

Teşekkür

D. radiodurans’ ın Dr[pUC8] ve Dr[pUC8:15] rekombinantları İnönü Üniversitesi Moleküler Biyoloji Bölümünde Prof. Dr. Hikmet Geçkil' in laboratuvarından temin edilmiştir. Ayrıca bu çalışma 2012-192 no’lu proje ile İnönü Üniversitesi Bilimsel Araştırma Projeleri tarafından desteklenmiştir.

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Elif Özbey ^*
0000-0001-7215-1922
Türkiye

Dilek Asma
0000-0002-3866-3016
Türkiye

Yayımlanma Tarihi

30 Haziran 2022

Gönderilme Tarihi

15 Nisan 2021

Kabul Tarihi

28 Temmuz 2021

Yayımlandığı Sayı

Yıl 2022 Cilt: 25 Sayı: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.916575

IZ

https://izlik.org/JA33RK49KF

Kaynak Göster

RIS / Bibtex

APA

Özbey, E., & Asma, D. (2022). UV-C’nin, Deinonoccus radiodurans ve Vitreoscilla Hemoglobin (vgb) Geni Aktarılmış Rekombinantlarında; SOD, KAT ve Karoten Miktarı Üzerine Etkisi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(3), 476-484. https://doi.org/10.18016/ksutarimdoga.vi.916575

In UV-C, Deinonoccus radiodurans and Vitreoscilla Hemoglobin (vgb) Gene Transferred Recombinants; Effect on SOD, KAT and Carotene Amount

Abstract

Keywords

UV-C’nin, Deinonoccus radiodurans ve Vitreoscilla Hemoglobin (vgb) Geni Aktarılmış Rekombinantlarında; SOD, KAT ve Karoten Miktarı Üzerine Etkisi

Öz

Anahtar Kelimeler

Destekleyen Kurum

Proje Numarası

Teşekkür

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

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