Efficacy of Entomopathogenic Fungi, Beauveria bassiana, Isaria fumosorosea and Metarhizium anisopliae Against Green Shield bug, Palomena prasina L. (Heteroptera: Pentatomidae)

Şeyma Yiğit; İslam Saruhan

doi:10.18016/ksutarimdoga.vi.938399

TR EN

Entomopatojenik funguslar; Beauveria bassiana, Isaria fumosorosea ve Metarhizium anisopliae’nın Palomena prasina L. (Heteroptera: Pentatomidae)’ya karşı etkileri

Öz

Bu çalışmada Beauveria bassiana‘’nın altı farklı ırkı), Isaria (Paecilomyces) fumosorosea ve Metarhizium anisopliae izolatlarının birer ırkı Palomena prasina L. (Heteroptera: Pentatomidae)’nın nimf ve erginlerine karşı laboratuvar (25±1 0C, % 70 nem ve 16:8 h (ışıklı: karanlık) ve arazi koşullarında biyolojik etkinlik denemeleri yapılmıştır. Ayrıca bazı izolatların laboratuvar şartlarında P. prasina’nın yumurtaları üzerine etkinliği belirlenmiştir. Laboratuvar çalışmalarında izolatların 1x108 konidi/mL spor yoğunluğu, 5’er adet ergin ve nimf kullanılmış ve çalışma 4 tekerrürlü olarak yürütülmüştür. Çalışma 14 gün boyunca takip edilerek yüzde ölüm oranları, LT50 ve LT95 değerleri belirlenmiştir. Çalışma sonucunda P. prasina’nın nimflerine karşı laboratuvar şartlarında kullanılan izolatların LT50 ve LT95 değerlerine bakıldığında sırasıyla; 4.19 - 7.11 gün ile 6.68 - 11.30 gün arasında bulunmuştur. Erginlerde ise LT50 ve LT95 değerlerine bakıldığında sırasıyla; 4.98 - 7.18 gün ve 8.03- 12.22 gün arasında belirlenmiştir. Arazi şartlarında ise nimflere karşı yapılan çalışmada izolatların LT50 ve LT95 değerlerine bakıldığında sırasıyla; 6.69 - 10.70 gün ve 10.29 -17.91 gün olarak belirlenmiştir. Erginlerde ise LT50 ve LT95 değerleri 7.29 - 10.70 gün ve 11.23 - 17.91 gün arasında değişmektedir. Yapılan çalışmada kullanılan tüm izolatların P. prasina ergin ve nimflerine karşı arazi şartlarındaki etkinliği labaoratuvar şartlarına nazaran daha düşük olmuştur. Sonuç olarak çalışmada kullanılan tüm izolatların P. prasina’ın ergin ve nimflerine karşı biyolojik mücadele de kullanılma potansiyeli sahip olduğu ortaya konulmuştur.

Anahtar Kelimeler

Efficacy of Entomopathogenic Fungi, Beauveria bassiana, Isaria fumosorosea and Metarhizium anisopliae Against Green Shield bug, Palomena prasina L. (Heteroptera: Pentatomidae)

Abstract

In this study, the entomopathogenic fungi; Beauveria bassiana, Isaria fumosorosea and Metarhizium anisopliae were evaluated against fourth nymphs and adults of the green shield bug, P. prasina. The experiment was conducted both under field and laboratory conditions. Isolates included six Beauveria bassiana, one Metarhizium anisopliae and one Isaria fumosorosea. Isolates were bioassayed against nymphs and adults in both under field and laboratory conditions. Four replications of the bioassays were analyzed and evaluated daily for 14 days. LT50 and LT95 values for the experimented isolates ranged from 4.19 to 7.11 days and from 6.68 to 11.30 days, respectively in laboratory for nymphs, ranged from 4.98 to 7.18 days and from 8.03 to 12.22 days, respectively in laboratory for adults. LT50 and LT95 values for the experimented isolates ranged from 6.69 to 10.70 days and from 10.29 to 17.91 days, respectively in field for nymphs, ranged from 7.29 to 10.70 days and from 11.23 to 17.91 days, respectively in field for adults. It was obvious that BB1/21b (Beauveria bassiana) was the most virulent on adults of P. prasina. As a result, while the effect of the isolates used in the study was high due to controlled conditions in the laboratory, it was low in field conditions.

Keywords

Destekleyen Kurum

OMÜ BAP

Proje Numarası

PYO.ZRT.1901.18.016

Teşekkür

My sincere thanks go to Prof. Dr. Fikret DEMIRCI (Ankara University, Faculty of Agriculture, Department of Plant Protection) and Associate Prof. Dr. Ismail ERPER (Ondokuz Mayıs University, Faculty of Agriculture, Department of Plant Protection). My other heartfelt appreciation goes to Ondokuz Mayıs University’s Scientific Research Project (BAP) (PROJECT NO: PYO.ZRT.1901.18.016) for the financial support.

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Ziraat, Veterinerlik ve Gıda Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Şeyma Yiğit ^*
0000-0003-2268-5103
Türkiye

İslam Saruhan
0000-0003-0229-9627
Türkiye

Yayımlanma Tarihi

31 Ekim 2022

Gönderilme Tarihi

17 Mayıs 2021

Kabul Tarihi

29 Eylül 2021

Yayımlandığı Sayı

Yıl 2022 Cilt: 25 Sayı: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.938399

IZ

https://izlik.org/JA39HK97GL

Kaynak Göster

RIS / Bibtex

APA

Yiğit, Ş., & Saruhan, İ. (2022). Efficacy of Entomopathogenic Fungi, Beauveria bassiana, Isaria fumosorosea and Metarhizium anisopliae Against Green Shield bug, Palomena prasina L. (Heteroptera: Pentatomidae). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(5), 1051-1060. https://doi.org/10.18016/ksutarimdoga.vi.938399