Effects of Callus Cell Suspension Cultures and Elicitor Applications on Bioactive Components in Globe Artichoke [Cynara cardunculus var. scolymus (L.) Fiori]

Tugce Ozsan; Ahmet Onus

doi:10.18016/ksutarimdoga.vi.952138

TR EN

Kallus Hücre Süspansiyon Kültürleri ve Elisitör Uygulamalarının Enginarda [Cynara cardunculus var. scolymus (L.) Fiori] Biyoaktif Bileşenler Üzerine Etkisi

Öz

Enginar [Cynara cardunculus var. scolymus (L.) Fiori] doğada ham bitki materyalinde yüksek miktarlarda ve stabil olarak üretilmeyen değerli biyoaktif bileşenleri nedeniyle sağlık üzerine koruyucu birçok özelliğe sahiptir. Bu biyoaktif bileşenler, kallus ve hücre süspansiyon kültürleri gibi değerli in vitro tekniklerin ve elisitör gibi çeşitli yeni uygulamaların katkısıyla artırılabilmektedir. Bu çalışmada, çeşitli besi ortam kombinasyonlarında kallus hücre süspansiyon kültürleri kullanılarak ve 3 farklı konsantrasyonda (metil jasmonat 50 µM, 100 µM ve 200 µM; kitosan 200 mg L-1, 400 mg L-1 ve 800 mg L-1) 3 farklı uygulama süresiyle (24 saat, 48 saat ve 72 saat) uygulamaların biyoaktif içerikler üzerine olan etkisi araştırılmıştır. Çeşitlerin biyoaktif bileşik profili HPLC-DAD ile belirlenmiştir. Elde edilen sonuçlar, besi ortamı bileşiminde iyi dengelenmiş oksin: sitokinin (1:1 veya 10:1) konsantrasyonlarının kullanılmasının, enginarda kallus oluşum sürecini tetiklemek için bir zorunluluk olduğunu açıkça ortaya koymuştur. Sonuçlar ayrıca biriken biyoaktif bileşenlerin ve miktarlarının çeşitlere göre değiştiğini ortaya koymuştur. Araştırma sonuçları, istenen biyoaktif bileşen seviyelerini elde etmek için metil jasmonat ve kitosan dışında farklı tipte elisitörlerin veya aynı elisitörlerin farklı dozlarının ve/veya uygulama sürelerinin kullanılması/test edilmesi gerektiğini açık bir şekilde ortaya koymuştur. Mevcut çalışmanın bulgularının, tarım ve ilaç mühendisliği gibi birçok önemli alanda destekleyici ve tamamlayıcı bir etkisi olacağı düşünülmektedir.

Anahtar Kelimeler

Effects of Callus Cell Suspension Cultures and Elicitor Applications on Bioactive Components in Globe Artichoke [Cynara cardunculus var. scolymus (L.) Fiori]

Abstract

Globe artichoke [Cynara cardunculus var. scolymus (L.) Fiori] has many health-protecting properties due to its valuable bioactive components which are not stable and produced in high amounts in the raw plant material in nature. These bioactive components that gaining interest can be increased thanks to the contribution of valuable in vitro techniques, such as callus and cell suspension cultures, and various new applications such as elicitor treatments. Present study aimed to determined bioactive components in three globe artichoke cultivars by using callus cell suspension cultures in various media combinations and by applying two elicitor treatments, namely methyl jasmonate and chitosan, at 3 different concentrations (methyl jasmonate 50 µM, 100 µM, and 200 µM; chitosan 200 mg L-1, 400 mg L-1, and 800 mg L-1) with 3 different application durations (24h, 48h, and 72h). The bioactive compounds profile of cultivars was determined by HPLC-DAD. Obtained results clearly revealed that using well-balanced concentrations of auxin: cytokinin (1:1 or 10:1) in a media composition is a must for triggering the callus formation process for globe artichoke. Results also showed that accumulated bioactive components and their amounts varied based on cultivars. Experiment results revealed that different types of elicitors other than methyl jasmonate and chitosan, or different doses of elicitors and application durations should be used/tested to get desired levels of bioactive components. The findings of the present study may play a supportively and complementarily mission in several important fields such as agriculture, and pharmaceutical engineering.

Keywords

Destekleyen Kurum

Akdeniz University Scientific Research Projects Coordination Unit

Proje Numarası

FDK-2019-4611 and FBA-2019-4814

Teşekkür

Thanks to Dr. Mehmet Ali Saridas from Cukurova University for his contribution to statistical analysis.

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Ziraat, Veterinerlik ve Gıda Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Tugce Ozsan ^*
0000-0002-3265-6886
Türkiye

Ahmet Onus
0000-0001-8615-1480
Türkiye

Yayımlanma Tarihi

30 Haziran 2022

Gönderilme Tarihi

14 Haziran 2021

Kabul Tarihi

17 Ağustos 2021

Yayımlandığı Sayı

Yıl 2022 Cilt: 25 Sayı: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.952138

IZ

https://izlik.org/JA92KR79EG

Kaynak Göster

RIS / Bibtex

APA

Ozsan, T., & Onus, A. (2022). Effects of Callus Cell Suspension Cultures and Elicitor Applications on Bioactive Components in Globe Artichoke [Cynara cardunculus var. scolymus (L.) Fiori]. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(3), 485-494. https://doi.org/10.18016/ksutarimdoga.vi.952138

Kallus Hücre Süspansiyon Kültürleri ve Elisitör Uygulamalarının Enginarda [Cynara cardunculus var. scolymus (L.) Fiori] Biyoaktif Bileşenler Üzerine Etkisi

Öz

Anahtar Kelimeler

Effects of Callus Cell Suspension Cultures and Elicitor Applications on Bioactive Components in Globe Artichoke [Cynara cardunculus var. scolymus (L.) Fiori]

Abstract

Keywords

Destekleyen Kurum

Proje Numarası

Teşekkür

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

e-ISSN: 2619-9149