Clinopodium serpyllifolium subsp. serpyllifolium Bitkisinin Akciğer Kanseri ve Beyin Glioma Hücre Hatlarında Antikanser, Antiproliferatif ve Apoptotik Hücre Ölümü Üzerine Etkilerinin Araştırılması

Sevgi Gezici; Didem Kocum; Fatih Yayla; Nazım Şekeroğlu

doi:10.18016/ksutarimdoga.vi.959044

EN TR

Investigation of Anticancer, Antiproliferative and Apoptotic Cell Death Effects of Clinopodium serpyllifolium subsp. serpyllifolium in Lung and Brain Glioma Cell Lines

Abstract

In this study, it was aimed to investigate cytotoxic, antiproliferative and apoptotic activities of Clinopodium serpyllifolium subsp. serpyllifolium, which grows naturally in the flora of Turkey and has been widely used in traditional folk medicine thanks to its rich phytochemical content. For this purpose, water (dH2O) ve 70% ethanol (C2H5OH-EtOH) extracts were obtained from flower and stems of the plants using different solvents. The activities of different extracts on the anticancer, antiproliferation and DNA damage-based apoptosis activities against A549, H1299, C6 and HUVEC cells using MTT, trypan blue and immunological-based ELISA methods, respectively. It was determined that plant extracts decreased viability of all the cells and induced these cells to die. The extracts showed the highest activity against the A549 lung carcinoma cells, whilst they showed the lowest activity against the C6 brain glioma cells. In conclusion, it is considered that different parts of the C. serpyllifolium should be phytochemically investigated in details, pharmacological and clinical studies ought to be carried out in order to use this plant as anticancer drug source.

Keywords

Clinopodium serpyllifolium subsp. serpyllifolium Bitkisinin Akciğer Kanseri ve Beyin Glioma Hücre Hatlarında Antikanser, Antiproliferatif ve Apoptotik Hücre Ölümü Üzerine Etkilerinin Araştırılması

Öz

Bu çalışmada, Türkiye florasında doğal olarak yetişen ve zengin fitokimyasal içeriği sayesinde geleneksel halk tıbbında yaygın olarak kullanılan Clinopodium serpyllifolium subsp. serpyllifolium bitkisinin sitotoksik, antiproliferatif ve apoptotik aktivitelerinin araştırılması amaçlanmıştır. Bu amaçla, bitkinin çiçek ve gövde kısımlarından su (dH2O) ve %70’lik etanol (C2H5OH-EtOH) çözücüleri kullanılarak ekstraktlar elde edilmiştir. Farklı ekstraktların antikanser, antiproliferizasyon ve DNA hasarı gelişimi üzerine apoptotik aktiviteleri; A549, H1299, C6 ve HUVEC hücrelerine karşı sırasıyla MTT, tripan mavisi ve immünolojik temelli ELISA yöntemleri kullanılarak analiz edilmiştir. Bitki ekstrelerinin, çalışılan kanser hücre hatlarının her üçünde de hücrelerdeki canlılık oranlarını azaltarak, hücreleri ölüme teşvik ettiği saptanmıştır. Ekstreler, en yüksek aktiviteyi A549 akciğer karsinoma hücrelerine karşı; en düşük aktiviteyi ise C6 beyin glioma hücreleri üzerine göstermiştir. Sonuç olarak, C. serpyllifolium bitkisinin çiçek ve gövde kısımlarının fitokimyasal içeriğinin belirlenmesi ve bitkinin antikanser drog olarak kullanımına yönelik farmakolojik ve klinik çalışmaların yapılması gerekmektedir.

Anahtar Kelimeler

Destekleyen Kurum

: Sunulan çalışma Kilis 7 Aralık Üniversitesi, Bilimsel Araştırma Projeleri (BAP) Yönetim Birimi tarafından finansal olarak desteklenmiştir.

Proje Numarası

Proje kodu: 1928MAP1

Teşekkür

Teşekkürler: Sunulan çalışma Kilis 7 Aralık Üniversitesi, Bilimsel Araştırma Projeleri (BAP) Yönetim birimi tarafından finansal olarak desteklenmiştir (Proje kodu: 1928MAP1).

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Sevgi Gezici ^*
0000-0002-4856-0221
Türkiye

Didem Kocum
0000-0003-2519-0608
Türkiye

Fatih Yayla
0000-0002-6490-6288
Türkiye

Nazım Şekeroğlu
0000-0002-0630-0106
Türkiye

Yayımlanma Tarihi

31 Ekim 2022

Gönderilme Tarihi

29 Haziran 2021

Kabul Tarihi

2 Eylül 2021

Yayımlandığı Sayı

Yıl 2022 Cilt: 25 Sayı: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.959044

IZ

https://izlik.org/JA73HA44UW

Kaynak Göster

RIS / Bibtex

APA

Gezici, S., Kocum, D., Yayla, F., & Şekeroğlu, N. (2022). Clinopodium serpyllifolium subsp. serpyllifolium Bitkisinin Akciğer Kanseri ve Beyin Glioma Hücre Hatlarında Antikanser, Antiproliferatif ve Apoptotik Hücre Ölümü Üzerine Etkilerinin Araştırılması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(5), 974-985. https://doi.org/10.18016/ksutarimdoga.vi.959044

Investigation of Anticancer, Antiproliferative and Apoptotic Cell Death Effects of Clinopodium serpyllifolium subsp. serpyllifolium in Lung and Brain Glioma Cell Lines

Abstract

Keywords

Clinopodium serpyllifolium subsp. serpyllifolium Bitkisinin Akciğer Kanseri ve Beyin Glioma Hücre Hatlarında Antikanser, Antiproliferatif ve Apoptotik Hücre Ölümü Üzerine Etkilerinin Araştırılması

Öz

Anahtar Kelimeler

Destekleyen Kurum

Proje Numarası

Teşekkür

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

e-ISSN: 2619-9149