Spirogyra aequinoctialis’in Farklı Çözgenler Ile Hazırlanan Ekstraktlarının Antibakteriyal ve Antifungal Etkileri

Köksal Pabuçcu; Tuğba Demiriz Yücer

doi:10.18016/ksutarimdoga.vi.979361

TR EN

Spirogyra aequinoctialis’in Farklı Çözgenler Ile Hazırlanan Ekstraktlarının Antibakteriyal ve Antifungal Etkileri

Öz

Bu çalışmada, sucul ortamlardan alınıp laboratuvar ortamına getirilerek izole edilen ve teşhis yöntemleri kullanarak tür tanımlaması yapılmış olan alg türü Spirogyra aequinoctialis ekstraktların çeşitli mikroorganizma türleri üzerinde antibakteriyal ve antifungal etkileri araştırılmıştır. Spirogyra ekstraktları, 0.5 M Tris-HCL pH:8.00, N-butanol ve Etanol ile hazırlanmıştır. Antimikrobiyal aktivite testleri, disk difüzyon yöntemi ile Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, Listeria monocytogenes ATCC 7644, Escherichia coli O 157:H7, Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium CCM 5445, Candida albicans ATCC 10239 mikroorganizmaları üzerinde denenmiştir. Antibakteriyal aktivite testleri ile, S. aequinoctialis’in tampon ekstraktının, Escherichia coli O 157:H7 üzerinde oldukça etkili antibakteriyal özelliğe sahip olduğu tespit edilmiştir. Staphylococcus aureus ATCC 25923 suşunun en duyarlı mikroorganizma, Pseudomonas aeruginosa ATCC 27853 suşunun ise en dirençli mikroorganizma olduğu; en etkili çözücünün 0.5 M Tris-HCL pH:8.00 olduğu kaydedilmiştir. Antifungal aktivite testleri ile S. aequinoctialis’in etanol ekstraktının, Candida albicans ATCC 10239 üzerinde oldukça etkili antifungal özelliğe sahip olduğu görülmüştür.

Anahtar Kelimeler

Antibacterial and Antifungal Effects of Extracts of Spirogyra aequinoctialis Prepared with Different Solvents

Abstract

In this study, the antibacterial and antifungal effects of algae species Spirogyra aequinoctialis extracts, which were taken from aquatic environments and brought to the laboratory environment and isolated by using diagnostic methods, on various microorganism species were investigated. Spirogyra extracts were prepared with 0.5 M Tris-HCL pH:8.00, N-butanol and Ethanol. Antimicrobial activities of the extracts were tested on Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, Listeria monocytogenes ATCC 7644, Escherichia coli O 157:H7, Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium ATCC, Candida albicans ATCC 10239 by disc diffusion method. As a result, it was determined that the buffer extract of S. aequinoctialis had highly effective antibacterial properties on Escherichia coli O 157:H7. Staphylococcus aureus ATCC 25923 strain was identified as the most susceptible microorganism, while Pseudomonas aeruginosa ATCC 27853 strain was the most resistant microorganism. It was also noted that the most effective solvent was 0.5 M Tris-HCL pH: 8.00. Antifungal activity tests showed that the ethanol extract of S. aequinoctialis had highly effective antifungal properties on Candida albicans ATCC 10239.

Keywords

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Köksal Pabuçcu
0000-0002-4473-8002
Türkiye

Tuğba Demiriz Yücer ^*
0000-0002-2494-4511
Türkiye

Yayımlanma Tarihi

31 Ekim 2022

Gönderilme Tarihi

5 Ağustos 2021

Kabul Tarihi

7 Ekim 2021

Yayımlandığı Sayı

Yıl 2022 Cilt: 25 Sayı: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.979361

IZ

https://izlik.org/JA88XU35WU

Kaynak Göster

RIS / Bibtex

APA

Pabuçcu, K., & Demiriz Yücer, T. (2022). Spirogyra aequinoctialis’in Farklı Çözgenler Ile Hazırlanan Ekstraktlarının Antibakteriyal ve Antifungal Etkileri. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(5), 927-932. https://doi.org/10.18016/ksutarimdoga.vi.979361

Spirogyra aequinoctialis’in Farklı Çözgenler Ile Hazırlanan Ekstraktlarının Antibakteriyal ve Antifungal Etkileri

Öz

Anahtar Kelimeler

Antibacterial and Antifungal Effects of Extracts of Spirogyra aequinoctialis Prepared with Different Solvents

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

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