Investigating In Vitro Genotoxic Effects of Sweetener Xylitol

Ece Avuloğlu Yılmaz; Deniz Yüzbaşıoğlu; Fatma Ünal

doi:10.18016/ksutarimdoga.vi.993825

EN TR

Investigating In Vitro Genotoxic Effects of Sweetener Xylitol

Abstract

Xylitol (XYL) is a sweetener used as a food additive in the food industry. In the present study, four different genotoxicity assays (chromosomal aberration=CA, sister chromatid exchange=SCE, cytokinesis-block micronucleus cytome=CBMN-Cyt, and comet assays) were conducted to assess the potential genotoxicity of XYL in human lymphocytes. Four concentrations (125, 250, 500, and 1000 μg mL-1) of XYL were applied to lymphocytes obtained from three healthy young donors. The frequency of CA was not significantly affected by 24-h administration of XYL (except 1000 μg mL-1 for the number of CAs). 48 h treatment of XYL increased the frequency of CAs and abnormal cells. However, this increase was significant at only two highest concentrations. XYL significantly increased SCE/cell rate at the two highest concentrations at both treatment times (24 and 48h). Similarly, the frequency of MN, Nuclear buds (NBUDs), and Nucleoplasmic bridges (NPBs) significantly increased by XYL only at the high concentrations. It raised the comet parameters at the two highest concentrations. These observations showed that XYL, only at high concentrations, may have a genotoxic effect on human lymphocytes in vitro. For this reason, it can be concluded that its use at low concentrations may not cause DNA or chromosomal damage.

Keywords

Tatlandırıcı Olan Xylitol'ün In Vitro Genotoksik Etkilerinin İncelenmesi

Öz

Xylitol (XYL), gıda endüstrisinde gıda katkı maddesi olarak kullanılan bir tatlandırıcıdır. Bu çalışmada, insan lenfositlerinde XYL'nin potansiyel genotoksisitesini değerlendirmek için dört farklı genotoksisite testi (kromozomal anormallik=KA, kardeş kromatid değişimi=KKD, sitokinez-blok mikronükleus sitom=CBMN-Cyt ve comet testleri) gerçekleştirilmiştir. Genç ve sağlıklı donörlerden elde edilen lenfositlere dört farklı konsantrasyonda (125, 250, 500 ve 1000 μg mL-1) XYL uygulanmıştır. KA frekansı, 24 saatlik XYL uygulamasından önemli ölçüde etkilenmemiştir (anormal hücre sayısı için 1000 μg mL-1 hariç). 48 saatlik XYL muamelesi, kromozomal anormalliklerin ve anormal hücrelerin sıklığını arttırmıştır. Ancak bu artış en yüksek iki konsantrasyonda anlamlıdır. XYL, tüm uygulama sürelerinde (24 ve 48 saat) en yüksek iki konsantrasyonda KKD/hücre oranını önemli ölçüde yükseltmiştir. Benzer şekilde, MN, Nükleer tomurcuklar (NBUD'ler) ve Nükleoplazmik köprülerin (NPB'ler) sıklığı, XYL’ün yüksek konsantrasyonlarında anlamlı artış göstermiştir. XYL comet parametrelerini en yüksek iki konsantrasyonda yükseltmiştir. Bu gözlemler, XYL'nin in vitro insan lenfositlerinde yalnızca yüksek konsantrasyonlarda genotoksik bir etkiye sahip olabileceğini göstermiştir. Bu nedenle düşük konsantrasyonlarda kullanımının DNA veya kromozomal hasara neden olmayabileceği düşünülmektedir.

Anahtar Kelimeler

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Ece Avuloğlu Yılmaz
0000-0002-5164-3431
Türkiye

Deniz Yüzbaşıoğlu ^*
0000-0003-2756-7712
Türkiye

Fatma Ünal
0000-0002-7468-6186
Türkiye

Yayımlanma Tarihi

30 Aralık 2022

Gönderilme Tarihi

10 Eylül 2021

Kabul Tarihi

26 Kasım 2021

Yayımlandığı Sayı

Yıl 2022 Cilt: 25 Sayı: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.993825

IZ

https://izlik.org/JA95HL94UY

Kaynak Göster

RIS / Bibtex

APA

Avuloğlu Yılmaz, E., Yüzbaşıoğlu, D., & Ünal, F. (2022). Investigating In Vitro Genotoxic Effects of Sweetener Xylitol. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(6), 1315-1325. https://doi.org/10.18016/ksutarimdoga.vi.993825